Competent Cells

Jusqu’au 31 janvier 2018, pour toute commande de bactéries compétentes NEB, recevez gratuitement l’ouvre-tube NEB. Limité à un par commande et jusqu’à épuisement des stocks.

Cloning Strains

For cloning experiments choose from several high efficiency competent cell strains. These E. coli strains are T1 phage resistant and are endA deficient for high quality plasmid preparations. Additionally, all competent cells from NEB are free of animal products.

Yeast

NEB offers chemically competent Kluyveromyces lactis cells and variants of this strain that have been tailored for specific protein expression needs. These cells are suitable for transformation with any of our linearized pKLAC series expression vectors.

E. coli for Protein Expression

NEB offers several strains with varying levels of expression control, each with T1 phage resistance and extremely high transformation efficiencies.

Competent E. coli – Competitor Cross-Reference Tool

Use this tool to select another company’s competent cell product and find out which NEB strain is compatible. Choose either the product name or catalog number from the available selection, and this tool will identify the recommended NEB product and its advantages. A link to the product page where you can also order the product is provided.
Competitor Cross-Reference Tool

Properties Table

Strain
Properties
NEB# Transform
Efficiency
(cfu/µg)(1)
Strain T1
Phage
Resistant
Library
Construc
-tion(2)
Cloning/
Sub-
cloning
Blue/
White
Screening
laclq lysY Endo-
nucleaseI
Deficient(3)
Protease(4)
Deficient
RecA¯ T7 RNA
Polyme
-rase
Cytoplasmic
disulfide bond
formation(5)
Drug
Resistance(6)
NEB Turbo C2984 1-3×109 K12 nit
NEB 5-alpha C2987 1-3×109 K12 none
NEB 5-alpha
F’Iq
C2992 1-3×109 K12 tet
NEB 10-beta C3019 1-3×109 K12 str
dam-/dcm- C2925 1-3×106 K12 cam, str, nit
NEB Stable C3040 1-3×109 K12 tet, str
Protein Expression Strain Properties
NEB Express C2523 0.6-1×109 B nit
NEB Express Iq C3037 0.6-1×109 B cam, nit
T7 Express C2566 0.6-1×109 B nit
T7 Express
lysY
C3010 0.6-1×109 B cam, nit
T7 Express
lysY/Iq
C3013 0.6-1×109 B cam, nit
T7 Express
Crystal
C3022 0.6-1×109 B nit
SHuffle T7 C3026 1 x 106 K12 str, spec
SHuffle
Express
C3028 1 x 107 B spec
SHuffle T7
Express
C3029 1 x 107 B spec(7)
SHuffle T7
Express lysY
C3030 1 x 107 B cam,
spec(7)
BL21 C2530 1-5×107 B
BL21(DE3) C2527 1-5×107 B
NiCo21(DE3) C2529 1-5×107 B
Lemo21(DE3) C2528 1-3×107 B cam

Plus d’informations disponibles dans la section Ressources Techniques ou sur neb.com