NEB offers several products that utilize secreted Gaussia Luciferase (GLuc) and Cypridina Luciferase (CLuc) in reporter assays. The sensitivity and secretory properties of these luciferases make them ideal for a number of applications. Gaussia and Cypridina are compatible and can be used alone or together as a dual system.
Gaussia Luciferase (GLuc) is a 20 kDa luciferase from the marine copepod Gaussia princeps.
This luciferase, which does not require ATP, catalyzes the oxidation of coelenterazine in a reaction that produces light (480 nm). GLuc has the highest reported activity for any characterized luciferase, is secreted from cells and offers a much higher bioluminescent signal than firefly (FLuc) and Renilla luciferases (RLuc).
Cypridina Luciferase (CLuc) is isolated from the marine ostracod Cypridina noctiluca. CLuc utilizes a vargulin luciferin and does not react with coelenterazine, allowing the detection of both GLuc and CLuc expressed from the same cells.
- Naturally Secreted – Amenable to live cell assays
- Sensitivity – Brightest luciferases available; enables single cell applications
- Stability – Samples can be stored for several days with no loss of activity
- Easy-to-use – Cell lysis not necessary
- Non-destructive – Living cells can be used in downstream assays
- Flexible – Activity can also be measured in cell lysates
BioLux® Luciferase Starter Kits
Luziferase Assay Kits
|pGLuc-Basic 2||N8082 S||–||Promoterless vectors for promoter evaluation and screening||Gaussia||20 µg||287 €|
|pGLuc-Mini-TK 2||N8086 S||Minimal||Minimal promoter for promoter or enhancer screening||Gaussia||20 µg||287 €|
|pTK-GLuc||N8084 S||Thymidin Kinase (TK)||Constitutive promoter, medium expression vector||Gaussia||20 µg||287 €|
|pCMV-GLuc 2||N8081 S||CMV||Constitutive promoter, high expression vector||Gaussia||20 µg||291 €|
|pCLuc-Basic 2||N0317 S||–||Promoterless vectors for promoter evaluation and screening||Cypridina||20 µg||281 €|
|pCLuc Mini-TK 2||N0324 S||Minimal||Promoterless vectors for promoter evaluation and screening||Cypridina||20 µg||282 €|
|pTK-CLuc Vektor||N0322 S||Thymidin Kinase (TK)||Constitutive promoter, medium expression vectorv||Cypridina||20 µg||276 €|
|pSV40-CLuc 2||N0318 S||SV40||Constitutive promoter, high expression vector||Cypridina||20 µg||281 €|