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Luciferase Reporter Systems

NEB offers several products that utilize secreted Gaussia Luciferase (GLuc) and Cypridina Luciferase (CLuc) in reporter assays. The sensitivity and secretory properties of these luciferases make them ideal for a number of applications. Gaussia and Cypridina are compatible and can be used alone or together as a dual system.

Gaussia Luciferase (GLuc) is a 20 kDa luciferase from the marine copepod Gaussia princeps.

This luciferase, which does not require ATP, catalyzes the oxidation of coelenterazine in a reaction that produces light (480 nm). GLuc has the highest reported activity for any characterized luciferase, is secreted from cells and offers a much higher bioluminescent signal than firefly (FLuc) and Renilla luciferases (RLuc).

Cypridina Luciferase (CLuc) is isolated from the marine ostracod Cypridina noctiluca. CLuc utilizes a vargulin luciferin and does not react with coelenterazine, allowing the detection of both GLuc and CLuc expressed from the same cells.

Advantages:

  • Naturally Secreted – Amenable to live cell assays
  • Sensitivity – Brightest luciferases available; enables single cell applications
  • Stability – Samples can be stored for several days with no loss of activity
  • Easy-to-use – Cell lysis not necessary
  • Non-destructive – Living cells can be used in downstream assays
  • Flexible – Activity can also be measured in cell lysates

Luciferase Assay Kits

PRODUCT Prod.Nr.: SIZE PRICE
BioLux® Gaussia Luciferase Assay Kit E3300S/L 100 Assays / 1000 Assays 93 € / 465 € NEB Shop_icon
BioLux® Cypridina Luciferase Assay Kit E3309S/L 100 Assays / 1000 Assays 108 € / 640 € NEB Shop_icon

Luciferase Vectors

Plasmid/ Vector Prod.Nr. Promoter Properties BioLux-System SIZE PRICE
pGLuc-Basic 2 N8082S Promoterless vectors for promoter evaluation and screening Gaussia 20 µg 304 € NEB Shop_icon
pGLuc-Mini-TK 2 N8086S Minimal Minimal promoter for promoter or enhancer screening Gaussia 20 µg 298 € NEB Shop_icon
pTK-GLuc N8084S Thymidin Kinase (TK) Constitutive promoter, medium expression vector Gaussia 20 µg 298 € NEB Shop_icon
pCMV-GLuc 2 N8081S CMV Constitutive promoter, high expression vector Gaussia 20 µg 308 € NEB Shop_icon
pCLuc-Basic 2 N0317S Promoterless vectors for promoter evaluation and screening Cypridina 20 µg 295 € NEB Shop_icon
pCLuc Mini-TK 2 N0324S Minimal Promoterless vectors for promoter evaluation and screening Cypridina 20 µg 295 € NEB Shop_icon
pTK-CLuc Vektor N0322S Thymidin Kinase (TK) Constitutive promoter, medium expression vectorv Cypridina 20 µg 290 € NEB Shop_icon
pSV40-CLuc 2 N0318S SV40 Constitutive promoter, high expression vector Cypridina 20 µg 295 € NEB Shop_icon

Plus d’informations disponibles dans la section Ressources Techniques ou sur neb.com