NEB offers several products that utilize secreted Gaussia Luciferase (GLuc) and Cypridina Luciferase (CLuc) in reporter assays. The sensitivity and secretory properties of these luciferases make them ideal for a number of applications. Gaussia and Cypridina are compatible and can be used alone or together as a dual system.
Gaussia Luciferase (GLuc) is a 20 kDa luciferase from the marine copepod Gaussia princeps.
This luciferase, which does not require ATP, catalyzes the oxidation of coelenterazine in a reaction that produces light (480 nm). GLuc has the highest reported activity for any characterized luciferase, is secreted from cells and offers a much higher bioluminescent signal than firefly (FLuc) and Renilla luciferases (RLuc).
Cypridina Luciferase (CLuc) is isolated from the marine ostracod Cypridina noctiluca. CLuc utilizes a vargulin luciferin and does not react with coelenterazine, allowing the detection of both GLuc and CLuc expressed from the same cells.
- Naturally Secreted – Amenable to live cell assays
- Sensitivity – Brightest luciferases available; enables single cell applications
- Stability – Samples can be stored for several days with no loss of activity
- Easy-to-use – Cell lysis not necessary
- Non-destructive – Living cells can be used in downstream assays
- Flexible – Activity can also be measured in cell lysates
Luciferase Assay Kits