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Luciferase Reporter Systems

NEB offers several products that utilize secreted Gaussia Luciferase (GLuc) and Cypridina Luciferase (CLuc) in reporter assays. The sensitivity and secretory properties of these luciferases make them ideal for a number of applications. Gaussia and Cypridina are compatible and can be used alone or together as a dual system.

Gaussia Luciferase (GLuc) is a 20 kDa luciferase from the marine copepod Gaussia princeps.

This luciferase, which does not require ATP, catalyzes the oxidation of coelenterazine in a reaction that produces light (480 nm). GLuc has the highest reported activity for any characterized luciferase, is secreted from cells and offers a much higher bioluminescent signal than firefly (FLuc) and Renilla luciferases (RLuc).

Cypridina Luciferase (CLuc) is isolated from the marine ostracod Cypridina noctiluca. CLuc utilizes a vargulin luciferin and does not react with coelenterazine, allowing the detection of both GLuc and CLuc expressed from the same cells.

Advantages:

  • Naturally Secreted – Amenable to live cell assays
  • Sensitivity – Brightest luciferases available; enables single cell applications
  • Stability – Samples can be stored for several days with no loss of activity
  • Easy-to-use – Cell lysis not necessary
  • Non-destructive – Living cells can be used in downstream assays
  • Flexible – Activity can also be measured in cell lysates

Luziferase Assay Kits

Assay Kit NEB# Kit includes Size Price
BioLux® Gaussia Luciferase Assay Kit NEB Shop_icon E3300 S/L Assay buffer and substrate for Gaussia (GLuc) Assays 100/1000 rxn 89/445 €
BioLux® Cypridina Luciferase Assay Kit NEB Shop_icon E3309 S/L Assay buffer and substrate for Cypridina (CLuc) Assays 100/1000 rxn 103/610 €

Luziferase Vektoren

Plasmid/ Vector NEB# Promoter Properties BioLux-System Size Price
pGLuc-Basic 2 NEB Shop_icon N8082 S Promoterless vectors for promoter evaluation and screening Gaussia 20 µg 287 €
pGLuc-Mini-TK 2 NEB Shop_icon N8086 S Minimal Minimal promoter for promoter or enhancer screening Gaussia 20 µg 287 €
pTK-GLuc NEB Shop_icon N8084 S Thymidin Kinase (TK) Constitutive promoter, medium expression vector Gaussia 20 µg 287 €
pCMV-GLuc 2 NEB Shop_icon N8081 S CMV Constitutive promoter, high expression vector Gaussia 20 µg 291 €
pCLuc-Basic 2 NEB Shop_icon N0317 S Promoterless vectors for promoter evaluation and screening Cypridina 20 µg 281 €
pCLuc Mini-TK 2 NEB Shop_icon N0324 S Minimal Promoterless vectors for promoter evaluation and screening Cypridina 20 µg 282 €
pTK-CLuc Vektor NEB Shop_icon N0322 S Thymidin Kinase (TK) Constitutive promoter, medium expression vectorv Cypridina 20 µg 276 €
pSV40-CLuc 2 NEB Shop_icon N0318 S SV40 Constitutive promoter, high expression vector Cypridina 20 µg 281 €

Plus d’informations disponibles dans la section Ressources Techniques ou sur neb.com