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NEW LunaScript™ RT SuperMix Kit

Two-step RT-qPCR uncouples cDNA synthesis and subsequent qPCR, allowing greater freedom in selecting reverse transcriptases (RTs) and qPCR reagents separately. This flexibility can be useful for controlling sequence representation, efficiency, and when working with difficult RT-qPCR reactions or low RNA inputs.

The latest addition to the Luna qPCR/RT-qPCR portfolio, the LunaScript RT SuperMix Kit is optimized for first strand cDNA synthesis in the context of a two-step RT-qPCR workflow. It delivers best-in-class performance, user-friendly protocols, and includes a convenient blue dye to track your sample.
The cDNA products generated by LunaScript have been extensively evaluated in qPCR using the Luna® qPCR Master Mixes. In combination, these products provide a two-step RT-qPCR workflow with excellent sensitivity and accurate, linear quantitations

Advantages:
  • Simplify reaction setup with convenient supermix format
  • Eliminate pipetting errors with non-interfering, visible tracking dye
  • Synthesize cDNA in less than 15 minutes
  • Experience best-in-class performance, as all Luna products have undergone rigorous testing to optimize specificity, sensitivity, accuracy and reproducibility
  • Enjoy consistent linearity, sensitivity, and capacity for reliable RNA quantification

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Combine LunaScript with Luna qPCR Master Mixes for robust RT-qPCR.

The LunaScript RT SuperMix Kit offers the shortest available first-strand cDNA synthesis protocol

Comparison of recommended protocols for cDNA synthesis. The LunaScript RT SuperMix Kit requires the shortest reaction time and tolerates elevated temperatures, reducing complications from RNA secondary structure.

The LunaScript RT SuperMix Kit offers exceptional sensitivity, linearity, and reproducibility in two-step RT-qPCR workflows

RNA was converted to cDNA using the 1X LunaScript RT SuperMix in 20 μl reactions using standard reaction conditions (25°C/2 min, 55°C/10 min, 95°C/1 min). cDNA was then quantitated by qPCR using the Luna Universal qPCR Master Mix (NEB #M3003) and 1 μl of cDNA product as template, with triplicate reactions at each input concentration. A. A serial dilution of Jurkat total RNA (1 μg – 1 pg) was converted to cDNA and then quantitated by qPCR using a β-actin target. B. ERCC (External RNA Controls Consortium) mix1 RNA containing 5×109 to 50 copies of ERCC00130 (~10 ng – 10 fg) was converted to cDNA and then quantitated by qPCR.

The LunaScript RT SuperMix Kit demonstrates superior linear detection of RNA targets

Commercially available cDNA supermixes were used according to manufacturer’s recommendations to generate cDNA from 1 μg – 100 pg human (Jurkat) total RNA. cDNA products were then evaluated by qPCR using eight targets varying in abundance, length and %GC. qPCR detection was performed using the Luna Universal qPCR Master Mix (NEB #M3003) or Luna Universal Probe qPCR Master Mix (NEB #M3004). Results were evaluated for efficiency and ΔCq, where ΔCq measures low input detection and lack of non-template control (NTC) amplification (ΔCq = average Cq of NTC – average Cq of lowest input). Green box indicates target performance criteria (Efficiency = 90-110%, ΔCq ≥ 3).

 

Available products

PRODUCT REF. SIZE PRICE MORE INFOS
Luna® Universal qPCR Master Mix M3003S 200 rxns (2x1ml) In den Shop wechseln Produktinformationen
M3003L 500 rxns (5x1ml) In den Shop wechseln Produktinformationen
M3003X 1000 rxns (10x1ml) In den Shop wechseln Produktinformationen
M3003E 2500 rxns (1x25ml) In den Shop wechseln Produktinformationen
Luna® Universal Probe qPCR Master Mix M3004S 200 rxns (2x1ml) In den Shop wechseln Produktinformationen
M3004L 500 rxns (5x1ml) In den Shop wechseln Produktinformationen
M3004X 1000 rxns (10x1ml) In den Shop wechseln Produktinformationen
M3004E 2500 rxns (1x25ml) In den Shop wechseln Produktinformationen
Luna® Universal One-Step RT-qPCR Kit E3005S 200 rxns In den Shop wechseln Produktinformationen
E3005L 500 rxns In den Shop wechseln Produktinformationen
E3005X 1000 rxns In den Shop wechseln Produktinformationen
E3005E 2500 rxns In den Shop wechseln Produktinformationen
Luna® Universal Probe One-Step RT-qPCR Kit E3006S 200 rxns In den Shop wechseln Produktinformationen
E3006L 500 rxns In den Shop wechseln Produktinformationen
E3006X 1000 rxns In den Shop wechseln Produktinformationen
E3006E 2500 rxns In den Shop wechseln Produktinformationen
NEW LunaScript RT SuperMix Kit E3010S 25 rxns In den Shop wechseln Produktinformationen
E3010L 100 rxns In den Shop wechseln Produktinformationen

Further information can be found in our Technical Resources section or at neb.com