1.0New England Biolabs Francehttps://www.neb-online.fr/en/rich600338<blockquote class="wp-embedded-content" data-secret="sELUOYkiMP"><a href="https://www.neb-online.fr/en/cloning-synthetic-biology/dna-modifying-enzymes/">DNA Modifying Enzymes</a></blockquote><iframe sandbox="allow-scripts" security="restricted" src="https://www.neb-online.fr/en/cloning-synthetic-biology/dna-modifying-enzymes/embed/#?secret=sELUOYkiMP" width="600" height="338" title="“DNA Modifying Enzymes” — New England Biolabs France" data-secret="sELUOYkiMP" frameborder="0" marginwidth="0" marginheight="0" scrolling="no" class="wp-embedded-content"></iframe><script type="text/javascript"> /* <![CDATA[ */ /*! This file is auto-generated */ !function(d,l){"use strict";l.querySelector&&d.addEventListener&&"undefined"!=typeof URL&&(d.wp=d.wp||{},d.wp.receiveEmbedMessage||(d.wp.receiveEmbedMessage=function(e){var t=e.data;if((t||t.secret||t.message||t.value)&&!/[^a-zA-Z0-9]/.test(t.secret)){for(var s,r,n,a=l.querySelectorAll('iframe[data-secret="'+t.secret+'"]'),o=l.querySelectorAll('blockquote[data-secret="'+t.secret+'"]'),c=new RegExp("^https?:$","i"),i=0;i<o.length;i++)o[i].style.display="none";for(i=0;i<a.length;i++)s=a[i],e.source===s.contentWindow&&(s.removeAttribute("style"),"height"===t.message?(1e3<(r=parseInt(t.value,10))?r=1e3:~~r<200&&(r=200),s.height=r):"link"===t.message&&(r=new URL(s.getAttribute("src")),n=new URL(t.value),c.test(n.protocol))&&n.host===r.host&&l.activeElement===s&&(d.top.location.href=t.value))}},d.addEventListener("message",d.wp.receiveEmbedMessage,!1),l.addEventListener("DOMContentLoaded",function(){for(var e,t,s=l.querySelectorAll("iframe.wp-embedded-content"),r=0;r<s.length;r++)(t=(e=s[r]).getAttribute("data-secret"))||(t=Math.random().toString(36).substring(2,12),e.src+="#?secret="+t,e.setAttribute("data-secret",t)),e.contentWindow.postMessage({message:"ready",secret:t},"*")},!1)))}(window,document); //# sourceURL=https://www.neb-online.fr/wp-includes/js/wp-embed.min.js /* ]]> */ </script> https://www.neb-online.fr/wp-content/uploads/2015/04/NEB_Klonierung_Header_klonierung_760px.jpg760200[vc_row][vc_column][vc_column_text] DNA Modification Producing DNA samples by shearing, nebulization, restriction enzyme digestion or PCR amplification frequently leaves DNA molecules with ends incompatible for downstream experiments. Selective enzymatic treatment is used to prepare DNA for ligation. Ligation, the subsequent step to DNA end modification in the cloning process, is the formation of covalent phosphodiester bonds between the 3′ hydroxyl and... Read more »