{"id":10618,"date":"2018-04-05T11:54:57","date_gmt":"2018-04-05T09:54:57","guid":{"rendered":"https:\/\/www.neb-online.fr\/\/en\/?page_id=10618"},"modified":"2022-11-14T14:30:15","modified_gmt":"2022-11-14T13:30:15","slug":"lunascript-rt-supermix-kit","status":"publish","type":"page","link":"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/luna-universal-qpcr-rt-qpcr\/lunascript-rt-supermix-kit\/","title":{"rendered":"LunaScript\u2122 RT SuperMix Kit"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]Two-step RT-qPCR uncouples cDNA synthesis and subsequent qPCR, allowing greater freedom in selecting reverse transcriptases (RTs) and qPCR reagents separately. This flexibility can be useful for controlling sequence representation, efficiency, and when working with difficult RT-qPCR reactions or low RNA inputs.<\/p>\n<p>The latest addition to the Luna qPCR\/RT-qPCR portfolio, the LunaScript RT SuperMix Kit is optimized for first strand cDNA synthesis in the context of a two-step RT-qPCR workflow. It delivers best-in-class performance, user-friendly protocols, and includes a convenient blue dye to track your sample.<br \/>\nThe cDNA products generated by LunaScript have been extensively evaluated in qPCR using the Luna\u00ae qPCR Master Mixes. In combination, these products provide a two-step RT-qPCR workflow with excellent sensitivity and accurate, linear quantitations<\/p>\n<p>[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/2&#8243;]<div class=\"vbox\">\n\t\t\t\t<span class=\"vhead\">Advantages:<\/span>\n\t\t\t\t\t<ul class=\"vlist\">\n\t\t\t\t\t\t<li><span>Simplify reaction setup with convenient supermix format<br \/><\/span><\/li><li><span>Eliminate pipetting errors with non-interfering, visible tracking dye<br \/><\/span><\/li><li><span>Synthesize cDNA in less than 15 minutes<br \/><\/span><\/li><li><span>Experience best-in-class performance, as all Luna products have undergone rigorous testing to optimize specificity, sensitivity, accuracy and reproducibility<br \/><\/span><\/li><li><span>Enjoy consistent linearity, sensitivity, and capacity for reliable RNA quantification<\/span><\/li>\n\t\t\t\t\t<\/ul>\n\t\t\t<\/div>[\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text][\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][vc_column_text]Do you want to try Luna products? Click below to request a sample! (maximum 2 different references per team)[\/vc_column_text]<a class=\"btn btn-standard form-btn\"  href=\"http:\/\/www.neb-online.fr\/?p=8621\" style=\"margin-right:auto;\">Request a free sample<\/a>[\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][vc_column_text]<\/p>\n<div class=\"vc_row wpb_row vc_row-fluid vc_custom_1520931911420 vc_row-has-fill\">\n<div class=\"wpb_column vc_column_container vc_col-sm-12\">\n<div class=\"vc_column-inner \">\n<div class=\"wpb_wrapper\">\n<div class=\"vc_toggle vc_toggle_round vc_toggle_color_sandy_brown vc_toggle_size_lg vc_custom_1521038048601\">\n<div class=\"vc_toggle_content\">\n<div id=\"wpcf7-f10866-p14738-o1\" class=\"wpcf7\" dir=\"ltr\" lang=\"de-DE\" role=\"form\">\n<form class=\"wpcf7-form\" action=\"\/en\/pcr-and-dna-amplification\/luna-universal-qpcr-rt-qpcr\/lunascript-rt-supermix-kit\/#wpcf7-f10866-p14738-o1\" method=\"post\" novalidate=\"novalidate\">\n<div class=\"wpcf7-response-output wpcf7-display-none\"><\/div>\n<\/form>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"vc_row wpb_row vc_row-fluid\">\n<div class=\"wpb_column vc_column_container vc_col-sm-12\">\n<div class=\"vc_column-inner \">\n<div class=\"wpb_wrapper\">\n<div class=\"wpb_text_column wpb_content_element \">\n<div class=\"wpb_wrapper\">\n<p><strong>Combine LunaScript with Luna qPCR Master Mixes for robust RT-qPCR.<\/strong><\/p>\n<\/div>\n<\/div>\n<div class=\"wpb_single_image wpb_content_element vc_align_left\">\n<figure class=\"wpb_wrapper vc_figure\"><a class=\"vc_single_image-wrapper vc_box_border_grey prettyphoto\" href=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2018\/03\/LunaScript_TwoStep_RTqPCR_0118.jpg\" target=\"_self\" rel=\"noopener noreferrer\" data-rel=\"prettyphoto[rel-14738-275102030] noopener noreferrer\"><img fetchpriority=\"high\" decoding=\"async\" class=\"vc_single_image-img attachment-full\" src=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2018\/03\/LunaScript_TwoStep_RTqPCR_0118.jpg\" sizes=\"(max-width: 1000px) 100vw, 1000px\" srcset=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2018\/03\/LunaScript_TwoStep_RTqPCR_0118.jpg 1000w, https:\/\/www.neb-online.de\/wp-content\/uploads\/2018\/03\/LunaScript_TwoStep_RTqPCR_0118-300x129.jpg 300w, https:\/\/www.neb-online.de\/wp-content\/uploads\/2018\/03\/LunaScript_TwoStep_RTqPCR_0118-768x329.jpg 768w\" alt=\"\" width=\"1000\" height=\"429\" \/><\/a><\/figure>\n<\/div>\n<div class=\"vc_separator wpb_content_element vc_separator_align_center vc_sep_width_100 vc_sep_pos_align_center vc_separator_no_text vc_sep_color_grey\"><\/div>\n<div class=\"wpb_text_column wpb_content_element \">\n<div class=\"wpb_wrapper\">\n<p><strong>The LunaScript RT SuperMix Kit offers the shortest available first-strand cDNA synthesis protocol<\/strong><\/p>\n<\/div>\n<\/div>\n<div class=\"wpb_single_image wpb_content_element vc_align_left\">\n<figure class=\"wpb_wrapper vc_figure\"><a class=\"vc_single_image-wrapper vc_box_border_grey prettyphoto\" href=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2018\/03\/Fig5_LunaScript_CyclingConditions_0118.jpg\" target=\"_self\" rel=\"noopener noreferrer\" data-rel=\"prettyphoto[rel-14738-84428674] noopener noreferrer\"><img decoding=\"async\" class=\"vc_single_image-img attachment-full\" src=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2018\/03\/Fig5_LunaScript_CyclingConditions_0118.jpg\" sizes=\"(max-width: 999px) 100vw, 999px\" srcset=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2018\/03\/Fig5_LunaScript_CyclingConditions_0118.jpg 999w, https:\/\/www.neb-online.de\/wp-content\/uploads\/2018\/03\/Fig5_LunaScript_CyclingConditions_0118-300x117.jpg 300w, https:\/\/www.neb-online.de\/wp-content\/uploads\/2018\/03\/Fig5_LunaScript_CyclingConditions_0118-768x300.jpg 768w\" alt=\"\" width=\"999\" height=\"390\" \/><\/a><\/figure>\n<\/div>\n<div class=\"wpb_text_column wpb_content_element \">\n<div class=\"wpb_wrapper\">\n<p><span class=\"bildunterschrift\">Comparison of recommended protocols for cDNA synthesis. The LunaScript RT SuperMix Kit requires the shortest reaction time and tolerates elevated temperatures, reducing complications from RNA secondary structure.<\/span><\/p>\n<\/div>\n<\/div>\n<div class=\"vc_separator wpb_content_element vc_separator_align_center vc_sep_width_100 vc_sep_pos_align_center vc_separator_no_text vc_sep_color_grey\"><\/div>\n<div class=\"wpb_text_column wpb_content_element \">\n<div class=\"wpb_wrapper\">\n<p><strong>The LunaScript RT SuperMix Kit offers exceptional sensitivity, linearity, and reproducibility in two-step RT-qPCR workflows<\/strong><\/p>\n<\/div>\n<\/div>\n<div class=\"wpb_single_image wpb_content_element vc_align_left\">\n<figure class=\"wpb_wrapper vc_figure\"><img decoding=\"async\" class=\"alignnone  wp-image-24173\" src=\"https:\/\/www.neb-online.fr\/wp-content\/uploads\/Fig1_LunaScript_RTqPCR.jpg-300x93.webp\" alt=\"\" width=\"650\" height=\"201\" srcset=\"https:\/\/www.neb-online.fr\/wp-content\/uploads\/Fig1_LunaScript_RTqPCR.jpg-300x93.webp 300w, https:\/\/www.neb-online.fr\/wp-content\/uploads\/Fig1_LunaScript_RTqPCR.jpg-1024x319.webp 1024w, https:\/\/www.neb-online.fr\/wp-content\/uploads\/Fig1_LunaScript_RTqPCR.jpg-768x239.webp 768w, https:\/\/www.neb-online.fr\/wp-content\/uploads\/Fig1_LunaScript_RTqPCR.jpg-1536x478.webp 1536w, https:\/\/www.neb-online.fr\/wp-content\/uploads\/Fig1_LunaScript_RTqPCR.jpg-2048x638.webp 2048w\" sizes=\"(max-width: 650px) 100vw, 650px\" \/><a class=\"vc_single_image-wrapper vc_box_border_grey prettyphoto\" href=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2018\/03\/Fig1_LunaScript_RTqPCR_0118.jpg\" data-rel=\"prettyPhoto[rel-14738-2131761364]\"><img loading=\"lazy\" decoding=\"async\" class=\"vc_single_image-img attachment-full alignnone\" src=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2018\/03\/Fig1_LunaScript_RTqPCR_0118.jpg\" sizes=\"(max-width: 999px) 100vw, 999px\" srcset=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2018\/03\/Fig1_LunaScript_RTqPCR_0118.jpg 999w, https:\/\/www.neb-online.de\/wp-content\/uploads\/2018\/03\/Fig1_LunaScript_RTqPCR_0118-300x93.jpg 300w, https:\/\/www.neb-online.de\/wp-content\/uploads\/2018\/03\/Fig1_LunaScript_RTqPCR_0118-768x239.jpg 768w\" alt=\"\" width=\"999\" height=\"311\" \/><\/a><\/figure>\n<\/div>\n<div class=\"wpb_text_column wpb_content_element \">\n<div class=\"wpb_wrapper\">\n<p><span class=\"bildunterschrift\">RNA was converted to cDNA using the 1X LunaScript RT SuperMix in 20 \u03bcl reactions using standard reaction conditions (25\u00b0C\/2 min, 55\u00b0C\/10 min, 95\u00b0C\/1 min). cDNA was then quantitated by qPCR using the Luna Universal qPCR Master Mix (NEB #M3003) and 1 \u03bcl of cDNA product as template, with triplicate reactions at each input concentration. A. A serial dilution of Jurkat total RNA (1 \u03bcg \u2013 1 pg) was converted to cDNA and then quantitated by qPCR using a \u03b2-actin target. B. ERCC (External RNA Controls Consortium) mix1 RNA containing 5\u00d7109 to 50 copies of ERCC00130 (~10 ng \u2013 10 fg) was converted to cDNA and then quantitated by qPCR.<\/span><\/p>\n<\/div>\n<\/div>\n<div class=\"wpb_text_column wpb_content_element \">\n<div class=\"wpb_wrapper\">\n<p><strong>The LunaScript RT SuperMix Kit demonstrates superior linear detection of RNA targets<\/strong><\/p>\n<\/div>\n<\/div>\n<div class=\"wpb_single_image wpb_content_element vc_align_left\">\n<figure class=\"wpb_wrapper vc_figure\">\n<div class=\"vc_single_image-wrapper vc_box_border_grey\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-medium wp-image-24169\" src=\"https:\/\/www.neb-online.fr\/wp-content\/uploads\/Fig4_LunaScript_LinearDetection-768x588-1-300x230.webp\" alt=\"\" width=\"300\" height=\"230\" srcset=\"https:\/\/www.neb-online.fr\/wp-content\/uploads\/Fig4_LunaScript_LinearDetection-768x588-1-300x230.webp 300w, https:\/\/www.neb-online.fr\/wp-content\/uploads\/Fig4_LunaScript_LinearDetection-768x588-1.webp 768w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"vc_single_image-img attachment-full\" src=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2018\/03\/Fig4_LunaScript_LinearDetection_0118.png\" sizes=\"(max-width: 520px) 100vw, 520px\" srcset=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2018\/03\/Fig4_LunaScript_LinearDetection_0118.png 520w, https:\/\/www.neb-online.de\/wp-content\/uploads\/2018\/03\/Fig4_LunaScript_LinearDetection_0118-300x230.png 300w\" alt=\"\" width=\"520\" height=\"398\" \/><\/div>\n<\/figure>\n<\/div>\n<div class=\"wpb_text_column wpb_content_element \">\n<div class=\"wpb_wrapper\">\n<p><span class=\"bildunterschrift\">Commercially available cDNA supermixes were used according to manufacturer\u2019s recommendations to generate cDNA from 1 \u03bcg \u2013 100 pg human (Jurkat) total RNA. cDNA products were then evaluated by qPCR using eight targets varying in abundance, length and %GC. qPCR detection was performed using the Luna Universal qPCR Master Mix (NEB #M3003) or Luna Universal Probe qPCR Master Mix (NEB #M3004). Results were evaluated for efficiency and \u0394Cq, where \u0394Cq measures low input detection and lack of non-template control (NTC) amplification (\u0394Cq = average Cq of NTC \u2013 average Cq of lowest input). Green box indicates target performance criteria (Efficiency = 90-110%, \u0394Cq \u2265 3).<\/span><\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"vc_row wpb_row vc_row-fluid\">\n<div class=\"wpb_column vc_column_container vc_col-sm-12\">\n<div class=\"vc_column-inner \">\n<div class=\"wpb_wrapper\">\n<div class=\"vc_separator wpb_content_element vc_separator_align_center vc_sep_width_100 vc_sep_pos_align_center vc_separator_no_text vc_sep_color_grey\">\n<p>&nbsp;<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<\/p>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<\/p>\n<div class=\"wpb_text_column wpb_content_element \">\n<div class=\"wpb_wrapper\">\n<h2>Available products<\/h2>\n<table style=\"width: 683px; height: 1161px;\" border=\"0\" cellpadding=\"5\">\n<tbody>\n<tr style=\"background-color: #f3f3f3; font-size: 16px;\">\n<th style=\"border-bottom: solid #eb7e2d;\" align=\"left\" width=\"300\" height=\"40px\">PRODUCT<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"88\">REF.<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"100\">SIZE<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" colspan=\"1\">PRICE<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" colspan=\"1\">MORE INFO<\/th>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"1\"><span style=\"color: #f58025;\"><strong>NEW <\/strong><\/span>LunaScript\u00ae RT Master Mix Kit (Primer-free)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E3025S\/L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">25\/100 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/neb-online.fr\/en\/art\/E3025@\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-4375 size-full\" src=\"https:\/\/www.neb-online.fr\/\/\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"In den Shop wechseln\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/international.neb.com\/products\/e3025-lunascript-rt-master-mix-kit-primer-free#Product%20Information\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-4375 size-full\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Information Produit\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"1\"><span style=\"color: #f58025;\"><strong>NEW <\/strong><\/span>Luna SARS-CoV-2 RT-qPCR Multiplex Assay<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E3019S\/L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">96\/480 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/neb-online.fr\/en\/art\/E3019@\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-4375 size-full\" src=\"https:\/\/www.neb-online.fr\/\/\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"In den Shop wechseln\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/international.neb.com\/products\/e3019-luna-sars-cov-2-rt-qpcr-multiplex-assay-kit#Product%20Information\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-4375 size-full\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Information Produit\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"1\"><span style=\"color: #f58025;\"><strong>NEW <\/strong><\/span>Luna Probe One-Step RT-qPCR 4x Mix with UDG<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M3019S\/L\/X\/E<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">200\/500\/1000\/2000 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/neb-online.fr\/en\/art\/M3019@\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-4375 size-full\" src=\"https:\/\/www.neb-online.fr\/\/\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"In den Shop wechseln\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/international.neb.com\/products\/m3019-luna-probe-one-step-rt-qpcr-4x-mix-with-udg#Product%20Information\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-4375 size-full\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Information Produit\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"1\">Luna Cell Ready One-Step RT-qPCR Kit<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E3030S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/neb-online.fr\/en\/art\/E3030S\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-4375 size-full\" src=\"https:\/\/www.neb-online.fr\/\/\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"In den Shop wechseln\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/products\/e3030-luna-cell-ready-one-step-rt-qpcr-kit#Product%20Information\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-4375 size-full\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Information Produit\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"1\">Luna Cell Ready Probe One-Step RT-qPCR Kit<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E3031S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/neb-online.fr\/en\/art\/E3031\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-4375 size-full\" src=\"https:\/\/www.neb-online.fr\/\/\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"Boutique en ligne\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/products\/e3031-luna-cell-ready-probe-one-step-rt-qpcr-kit#Product%20Information\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-4375 size-full\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Information Produit\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"1\">Luna Cell Ready Lysis Module<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E3032S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/neb-online.fr\/en\/art\/E3032S\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-4375 size-full\" src=\"https:\/\/www.neb-online.fr\/\/\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"Boutique en ligne\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/products\/e3032-luna-cell-ready-lysis-module#Product%20Information\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-4375 size-full\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Information Produit\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"4\">Luna Universal qPCR Master Mix<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M3003S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">200 rxns (2x1ml)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/neb-online.fr\/en\/art\/M3003S\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4375 size-full aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"http:\/\/international.neb.com\/products\/m3003-luna-universal-qpcr-master-mix\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4375 size-full aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M3003L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">500 rxns (5x1ml)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/neb-online.fr\/en\/art\/M3003L\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4375 size-full aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"http:\/\/international.neb.com\/products\/m3003-luna-universal-qpcr-master-mix\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4375 size-full aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M3003X<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">1000 rxns (10x1ml)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/neb-online.fr\/en\/art\/M3003X\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4375 size-full aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"http:\/\/international.neb.com\/products\/m3003-luna-universal-qpcr-master-mix\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4375 size-full aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M3003E<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">2500 rxns (1x25ml)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/neb-online.fr\/en\/art\/M3003E\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4375 size-full aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"http:\/\/international.neb.com\/products\/m3003-luna-universal-qpcr-master-mix\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4375 size-full aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"4\">Luna Universal Probe qPCR Master Mix<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M3004S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">200 rxns (2x1ml)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/neb-online.fr\/en\/art\/M3004S\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4375 size-full aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"http:\/\/international.neb.com\/products\/m3004-luna-universal-probe-qpcr-master-mix\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4375 size-full aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M3004L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">500 rxns (5x1ml)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/neb-online.fr\/en\/art\/M3004L\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4375 size-full aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"http:\/\/international.neb.com\/products\/m3004-luna-universal-probe-qpcr-master-mix\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4375 size-full aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M3004X<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">1000 rxns (10x1ml)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/neb-online.fr\/en\/art\/M3004X\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4375 size-full aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"http:\/\/international.neb.com\/products\/m3004-luna-universal-probe-qpcr-master-mix\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4375 size-full aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M3004E<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">2500 rxns (1x25ml)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/neb-online.fr\/en\/art\/M3004E\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4375 size-full aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"http:\/\/international.neb.com\/products\/m3004-luna-universal-probe-qpcr-master-mix\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4375 size-full aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"1\">Luna Universal One-Step RT-qPCR Kit<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E3005S\/L\/X\/E<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">200\/500\/1000\/2500 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/neb-online.fr\/en\/art\/E3005S\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4375 size-full aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"http:\/\/international.neb.com\/products\/e3005-luna-universal-one-step-rt-qpcr-kit\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4375 size-full aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"1\">Luna Universal Probe One-Step RT-qPCR Kit<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E3006S\/L\/X\/E<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">200\/500\/1000\/2500 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/neb-online.fr\/en\/art\/E3006S\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4375 size-full aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"http:\/\/international.neb.com\/products\/e3006-luna-universal-probe-one-step-rt-qpcr-kit\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4375 size-full aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"1\">LunaScript RT SuperMix Kit<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E3010S\/L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">25\/100 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/neb-online.fr\/en\/art\/E3010S\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4375 size-full aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/international.neb.com\/products\/e3010-luna-script-rt-supermix-kit#Product%20Information\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4375 size-full aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<div class=\"wpb_column vc_column_container vc_col-sm-12\">\n<div class=\"vc_column-inner \">\n<div class=\"wpb_wrapper\">\n<div class=\"wpb_text_column wpb_content_element \">\n<div class=\"wpb_wrapper\">\n<div class=\"wpb_column vc_column_container vc_col-sm-12\">\n<div class=\"vc_column-inner \">\n<div class=\"wpb_wrapper\">\n<div class=\"wpb_text_column wpb_content_element \">\n<div class=\"wpb_wrapper\"><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<\/p>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<\/p>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<\/p>\n<p>[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]Two-step RT-qPCR uncouples cDNA synthesis and subsequent qPCR, allowing greater freedom in selecting reverse transcriptases (RTs) and qPCR reagents separately. This flexibility can be useful for controlling sequence representation, efficiency, and when working with difficult RT-qPCR reactions or low RNA inputs. The latest addition to the Luna qPCR\/RT-qPCR portfolio, the LunaScript RT SuperMix Kit&#8230;  <a class=\"excerpt-read-more\" href=\"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/luna-universal-qpcr-rt-qpcr\/lunascript-rt-supermix-kit\/\" title=\"Read LunaScript\u2122 RT SuperMix Kit\">Read more &raquo;<\/a><\/p>\n","protected":false},"author":15,"featured_media":8664,"parent":8731,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-sidebar-pcr-dna-amplification.php","meta":{"footnotes":""},"class_list":["post-10618","page","type-page","status-publish","has-post-thumbnail","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.0 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>LunaScript\u2122 RT SuperMix Kit - New England Biolabs France<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/luna-universal-qpcr-rt-qpcr\/lunascript-rt-supermix-kit\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"LunaScript\u2122 RT SuperMix Kit - New England Biolabs France\" \/>\n<meta property=\"og:description\" content=\"[vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]Two-step RT-qPCR uncouples cDNA synthesis and subsequent qPCR, allowing greater freedom in selecting reverse transcriptases (RTs) and qPCR reagents separately. This flexibility can be useful for controlling sequence representation, efficiency, and when working with difficult RT-qPCR reactions or low RNA inputs. The latest addition to the Luna qPCR\/RT-qPCR portfolio, the LunaScript RT SuperMix Kit... 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