{"id":12315,"date":"2019-07-09T15:58:48","date_gmt":"2019-07-09T13:58:48","guid":{"rendered":"https:\/\/www.neb-online.fr\/\/en\/neb-en\/pcr-and-dna-amplification\/isothermal-amplification-2\/"},"modified":"2022-11-14T14:19:07","modified_gmt":"2022-11-14T13:19:07","slug":"isothermal-amplification-2","status":"publish","type":"page","link":"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/isothermal-amplification-2\/","title":{"rendered":"Isothermal Amplification"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row css=&#8221;.vc_custom_1448619604073{margin-right: 5px !important;}&#8221;][vc_column][vc_row_inner][vc_column_inner][vc_column_text]Isothermal Amplification is becoming more and more popular as a simple and cost effective alternative to standard PCR.[\/vc_column_text][vc_column_text]<span style=\"font-size: 1.3em;\"><strong>Your general advantages:<\/strong><\/span><\/p>\n<ul>\n<li style=\"font-size: 1.2em;\">No PCR Cycler necessary<\/li>\n<li style=\"font-size: 1.2em;\">Fast protocols<\/li>\n<li style=\"font-size: 1.2em;\">High reliability &amp; specificity<\/li>\n<\/ul>\n<p>[\/vc_column_text][vc_column_text css=&#8221;.vc_custom_1448631693081{margin-top: 10px !important;}&#8221;]The most famous &#8220;isoAmp&#8221; is LAMP.[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_separator color=&#8221;black&#8221;][\/vc_column][\/vc_row][vc_row css=&#8221;.vc_custom_1448617936808{margin-top: 30px !important;margin-bottom: 5px !important;}&#8221;][vc_column css=&#8221;.vc_custom_1448614738941{border-top-width: 2px !important;border-right-width: 0px !important;border-bottom-width: 2px !important;border-left-width: 0px !important;}&#8221;][vc_column_text]<\/p>\n<h2>Videos, Schematic illustration<\/h2>\n<p>[\/vc_column_text][vc_column_text]<span style=\"font-size: 1.3em; color: #dd7500;\"><strong>So what is LAMP (Loop-mediated isothermal Amplification) exactly?<\/strong><\/span>[\/vc_column_text][vc_row_inner][vc_column_inner width=&#8221;2\/3&#8243;][vc_video link=&#8221;https:\/\/youtu.be\/L5zi2P4lggw&#8221;][\/vc_column_inner][vc_column_inner width=&#8221;1\/3&#8243;][vc_column_text]Did you know that this method of Isothermal Amplification can be performed in only 5-10 minutes without big technical equipment like a PCR Cycler or similar devices?<\/p>\n<p>Learn about this and more in our new video-tutorial <strong>&#8220;Overview of Loop-mediated isothermal Amplification&#8221;.<\/strong>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row css=&#8221;.vc_custom_1448617723904{margin-top: 50px !important;margin-bottom: 50px !important;}&#8221;][vc_column][vc_separator color=&#8221;black&#8221;][vc_column_text]<strong>Schematic illustration LAMP<\/strong>[\/vc_column_text][vc_row_inner][vc_column_inner width=&#8221;1\/2&#8243;][vc_column_text]<span class=\"bildunterschrift\"><strong>Loop-mediated isothermal amplification (LAMP)<\/strong> uses 4-6 primers recognizing 6-8 distinct regions of target DNA. A strand-displacing DNA polymerase initiates synthesis and 2 of the primers form loop structures to facilitate subsequent rounds of amplification.<\/span>[\/vc_column_text][\/vc_column_inner][vc_column_inner width=&#8221;1\/2&#8243;][vc_single_image image=&#8221;12304&#8243; img_size=&#8221;full&#8221; alignment=&#8221;center&#8221; style=&#8221;vc_box_shadow&#8221; onclick=&#8221;img_link_large&#8221;][\/vc_column_inner][\/vc_row_inner][vc_separator color=&#8221;black&#8221;][vc_row_inner equal_height=&#8221;yes&#8221;][vc_column_inner width=&#8221;1\/2&#8243;][vc_column_text][\/vc_column_text][vc_column_text]<a href=\"https:\/\/international.neb.com\/tools-and-resources\/video-library?device=modal&amp;videoid={C8073389-95AA-4ECC-8828-C784AE8B3AB2}\"><img fetchpriority=\"high\" decoding=\"async\" class=\"size-full wp-image-7161 aligncenter\" src=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/04\/video_thumb_4.jpg\" alt=\"Video thumb\" width=\"300\" height=\"192\" \/><\/a>[\/vc_column_text]<a class=\"btn btn-standard form-btn\"  href=\"https:\/\/international.neb.com\/tools-and-resources\/video-library?device=modal&amp;videoid={C8073389-95AA-4ECC-8828-C784AE8B3AB2}\" style=\"margin-left:auto;margin-right:auto;\">Watch video<\/a>[vc_column_text css=&#8221;.vc_custom_1448630362645{padding-right: 30px !important;padding-left: 30px !important;}&#8221;]NEB Scientists have recently published a well respected paper on highly-specific detection of pathogens of the tropical disease &#8220;river blindness&#8221; through LAMP and a simple color change.[\/vc_column_text][\/vc_column_inner][vc_column_inner width=&#8221;1\/2&#8243; css=&#8221;.vc_custom_1448616568252{background-color: Mittels eines einfachen Farbumschlags die erfolgreiche isothermale Amplifikation visualisieren! Wie geht das? !important;}&#8221;][vc_column_text]<a href=\"https:\/\/www.neb.com\/tools-and-resources\/video-library?device=modal&amp;videoid={E5D366A3-C28E-49FC-99D4-558A0A671BDA}\"><img decoding=\"async\" class=\"size-full wp-image-7160 aligncenter\" src=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/04\/video_thumb_3.jpg\" alt=\"Video thumb\" width=\"300\" height=\"192\" \/><\/a>[\/vc_column_text]<a class=\"btn btn-standard form-btn\"  href=\"https:\/\/www.neb.com\/tools-and-resources\/video-library?device=modal&amp;videoid={E5D366A3-C28E-49FC-99D4-558A0A671BDA}\" style=\"margin-left:auto;margin-right:auto;\">Watch video<\/a>[vc_column_text css=&#8221;.vc_custom_1448631822978{padding-right: 30px !important;padding-left: 30px !important;}&#8221;]Visualizing the successful Isothermal Amplification using a simple color change! How does it work?[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_separator color=&#8221;black&#8221;][vc_column_text css=&#8221;.vc_custom_1554393446914{margin-bottom: 30px !important;padding-top: 20px !important;padding-bottom: 20px !important;padding-left: 20px !important;background-color: #f4f1e7 !important;}&#8221;]<\/p>\n<p style=\"text-align: center;\"><strong><span style=\"font-size: 1.3em;\">For more information on LAMP, please visit also <a href=\"https:\/\/international.neb.com\/applications\/dna-amplification-pcr-and-qpcr\/isothermal-amplification#tabselect0\" target=\"_blank\" rel=\"noopener\">www.neb.com<\/a><\/span><\/strong><\/p>\n<p>[\/vc_column_text][vc_separator color=&#8221;black&#8221;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;2\/3&#8243;][vc_column_text]<\/p>\n<h2>WarmStart LAMP Kits<\/h2>\n<p>For simple and reliable LAMP experiments as an alternative to PCR we now offer <a href=\"https:\/\/neb-online.fr\/en\/art\/E1700@\">WarmStart LAMP Kit<\/a> (DNA &amp; RNA) and <a href=\"https:\/\/neb-online.fr\/en\/art\/M1800@\">WarmStart Colorimetric LAMP 2X Master Mix<\/a> (DNA &amp; RNA).<\/p>\n<p>These Master Mixes contain &#8211; by NEB&#8217;s scientists <em>in silico<\/em> created, unique Designer Versions of Bst DNA Polymerase and a Reverse Transcriptase \u2013 optimized fpr Loop-mediated Isothermal Amplification on DNA and RNA.<\/p>\n<p><strong>The new WarmStart LAMP 2X Master Mixes (DNA &amp; RNA) are best suited as a fast and reliable analyzing method alternative for PCR in any Molecular Biology lab as well as in the field or DNA tests outside the lab.<br \/>\n<\/strong>[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/3&#8243;]<div class=\"vbox\">\n\t\t\t\t<span class=\"vhead\">Advantages:<\/span>\n\t\t\t\t\t<ul class=\"vlist\">\n\t\t\t\t\t\t<li><span>Reliable amplification of DNA and RNA templates in only 5-50 minutes!<br \/><\/span><\/li><li><span>Simple, isothermal application without a PCR Cycler.<br \/><\/span><\/li><li><span>Detection of amplification through fluorescence (#E1700) or by eye through color change (#M1800).<\/span><\/li>\n\t\t\t\t\t<\/ul>\n\t\t\t<\/div>[\/vc_column][\/vc_row][vc_row][vc_column][vc_separator color=&#8221;black&#8221;][vc_column_text]<\/p>\n<h2>Product table<\/h2>\n<p>&nbsp;<\/p>\n<table style=\"width: 760px;\" border=\"0\" cellpadding=\"5\">\n<tbody>\n<tr style=\"background-color: #f3f3f3; font-size: 16px;\">\n<th style=\"border-bottom: solid #eb7e2d;\" align=\"left\" width=\"400\" height=\"40px\">PRODUCT<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"88\">Prod. #<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"100\">SIZE<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" colspan=\"2\">PRICE<\/th>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">WarmStart<sup>\u00ae<\/sup> LAMP Kit (DNA &amp; RNA)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E1700S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/neb-online.fr\/en\/art\/E1700@\" target=\"_blank\" rel=\"noopener\"><img decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"http:\/\/international.neb.com\/products\/e1700-warmstart-lamp-kit-dna-rna\" target=\"_blank\" rel=\"noopener\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">WarmStart<sup>\u00ae<\/sup> LAMP Kit (DNA &amp; RNA)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E1700L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">500 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/neb-online.fr\/en\/art\/E1700@\" target=\"_blank\" rel=\"noopener\"><img decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"http:\/\/international.neb.com\/products\/e1700-warmstart-lamp-kit-dna-rna\" target=\"_blank\" rel=\"noopener\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">WarmStart<sup>\u00ae<\/sup> Colorimetric LAMP 2X Master Mix (DNA &amp; RNA)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M1800S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/neb-online.fr\/en\/art\/M1800@\" target=\"_blank\" rel=\"noopener\"><img decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"http:\/\/international.neb.com\/M1800\" target=\"_blank\" rel=\"noopener\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">WarmStart<sup>\u00ae<\/sup> Colorimetric LAMP 2X Master Mix (DNA &amp; RNA)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M1800L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">500 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/neb-online.fr\/en\/art\/M1800@\" target=\"_blank\" rel=\"noopener\"><img decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"http:\/\/international.neb.com\/M1800\" target=\"_blank\" rel=\"noopener\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p style=\"text-align: right;\"><span class=\"bildunterschrift\">As of: 03.01.2019<\/span><\/p>\n<p>[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row css=&#8221;.vc_custom_1448619604073{margin-right: 5px !important;}&#8221;][vc_column][vc_row_inner][vc_column_inner][vc_column_text]Isothermal Amplification is becoming more and more popular as a simple and cost effective alternative to standard PCR.[\/vc_column_text][vc_column_text]Your general advantages: No PCR Cycler necessary Fast protocols High reliability &amp; specificity [\/vc_column_text][vc_column_text css=&#8221;.vc_custom_1448631693081{margin-top: 10px !important;}&#8221;]The most famous &#8220;isoAmp&#8221; is LAMP.[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_separator color=&#8221;black&#8221;][\/vc_column][\/vc_row][vc_row css=&#8221;.vc_custom_1448617936808{margin-top: 30px !important;margin-bottom: 5px !important;}&#8221;][vc_column css=&#8221;.vc_custom_1448614738941{border-top-width: 2px !important;border-right-width: 0px !important;border-bottom-width: 2px !important;border-left-width:&#8230;  <a class=\"excerpt-read-more\" href=\"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/isothermal-amplification-2\/\" title=\"Read Isothermal Amplification\">Read more &raquo;<\/a><\/p>\n","protected":false},"author":15,"featured_media":12307,"parent":2092,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-sidebar-pcr-dna-amplification.php","meta":{"footnotes":""},"class_list":["post-12315","page","type-page","status-publish","has-post-thumbnail","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.5 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Isothermal Amplification - New England Biolabs France<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/isothermal-amplification-2\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Isothermal Amplification - New England Biolabs France\" \/>\n<meta property=\"og:description\" content=\"[vc_row css=&#8221;.vc_custom_1448619604073{margin-right: 5px !important;}&#8221;][vc_column][vc_row_inner][vc_column_inner][vc_column_text]Isothermal Amplification is becoming more and more popular as a simple and cost effective alternative to standard PCR.[\/vc_column_text][vc_column_text]Your general advantages: No PCR Cycler necessary Fast protocols High reliability &amp; specificity [\/vc_column_text][vc_column_text css=&#8221;.vc_custom_1448631693081{margin-top: 10px !important;}&#8221;]The most famous &#8220;isoAmp&#8221; is LAMP.[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_separator color=&#8221;black&#8221;][\/vc_column][\/vc_row][vc_row css=&#8221;.vc_custom_1448617936808{margin-top: 30px !important;margin-bottom: 5px !important;}&#8221;][vc_column css=&#8221;.vc_custom_1448614738941{border-top-width: 2px !important;border-right-width: 0px !important;border-bottom-width: 2px !important;border-left-width:... 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