{"id":12389,"date":"2019-07-18T13:56:09","date_gmt":"2019-07-18T11:56:09","guid":{"rendered":"https:\/\/www.neb-online.fr\/\/en\/neb-en\/pcr-and-dna-amplification\/exo-cip-rapid-pcr-cleanup-kit\/"},"modified":"2022-11-14T14:22:07","modified_gmt":"2022-11-14T13:22:07","slug":"exo-cip-rapid-pcr-cleanup-kit","status":"publish","type":"page","link":"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/exo-cip-rapid-pcr-cleanup-kit\/","title":{"rendered":"Exo-CIP Rapid PCR Cleanup Kit"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row][vc_column][vc_column_text]<\/p>\n<h2>Fast and convenient PCR Cleanup<\/h2>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]The Exo-CIP Rapid PCR Cleanup Kit is the ideal solution for convenient PCR purification.<\/p>\n<p>The Exo-CIP PCR purification kit contains optimized formulations of two enzymes. The thermolabile Exonuclease I digests excess primers while the thermolabile CIP (Calf Intestinal Phosphatase) dephosphorylates excess dNTPs. Both enzymes can be heat-inactivated in a minute, no further purification of the PCR products is required.[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/2&#8243;]<div class=\"vbox\">\n\t\t\t\t<span class=\"vhead\">Advantages:<\/span>\n\t\t\t\t\t<ul class=\"vlist\">\n\t\t\t\t\t\t<li><span>Rapidly degrade residual PCR primers and dephosphorylate excess dNTPs after amplification<br \/><\/span><\/li><li><span>Reaction complete in 4 minutes<br \/><\/span><\/li><li><span>Thermolabile formulation can be heat inactivated in 1 minute at 80\u00b0C<br \/><\/span><\/li><li><span>PCR product can be used directly in downstream applications<br \/><\/span><\/li><li><span>Compatible with commonly-used reaction buffers<\/span><\/li>\n\t\t\t\t\t<\/ul>\n\t\t\t<\/div>[\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text][\/vc_column_text][vc_separator color=&#8221;custom&#8221; accent_color=&#8221;#dd9933&#8243;][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<\/p>\n<h2 style=\"text-align: center;\">Want to try it?<\/h2>\n<p>[\/vc_column_text]<a class=\"btn btn-standard form-btn\" target=\"_blank\" href=\"https:\/\/international.neb.com\/forms\/exo-cip-rapid-pcr-cleanup-kit-sample-request\" style=\"margin-left:auto;margin-right:auto;\">Request a sample!<\/a>[\/vc_column][\/vc_row][vc_row][vc_column][vc_separator color=&#8221;custom&#8221; accent_color=&#8221;#dd9933&#8243;][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<\/p>\n<h2>Quick and easy PCR Purification with the Exo-CIP Rapid PCR Cleanup Kit<\/h2>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]The enzymes are added to the PCR reaction and incubated at 37\u00b0C for 4 minutes.<\/p>\n<p>The temperature is then raised to 80\u00b0C and the enzymes are completely inactivated within one minute.<\/p>\n<p>The purified PCR product is available for the following applications.<\/p>\n<p>The Exo-CIP Rapid PCR Cleanup Kit is compatible with all common PCR buffers.[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/2&#8243;][vc_single_image image=&#8221;12374&#8243; img_size=&#8221;medium&#8221; alignment=&#8221;center&#8221;][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator color=&#8221;custom&#8221; accent_color=&#8221;#dd9933&#8243;][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<\/p>\n<h2>Exo-CIP Rapid PCR Cleanup Kit enables consistent read lengths for amplicons of varying length<\/h2>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/2&#8243;][vc_single_image image=&#8221;12375&#8243; img_size=&#8221;full&#8221; alignment=&#8221;center&#8221;][\/vc_column][vc_column width=&#8221;1\/2&#8243;][vc_column_text]<span class=\"bildunterschrift\">PCR products of varying lengths were treated with Exo-CIP Rapid PCR Cleanup Kit or ExoSAP-IT PCR Cleanup Reagent following manufacturer-recommended protocols (5 min vs. 30 min). 3 \u00b5l of treated samples was used as input for Sanger sequencing on an ABI 3730 DNA Analyzer using BigDye Terminator chemistry (v3.1). Samples treated with Exo-CIP Rapid PCR Cleanup Kit yielded read lengths over 650 bases, matching samples treated with ExoSAP-IT PCR Cleanup Reagent.<\/span>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator color=&#8221;custom&#8221; accent_color=&#8221;#dd9933&#8243;][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<\/p>\n<h2>Comparison of sequencing results with Exo-CIP Rapid PCR Cleanup Kit<\/h2>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_single_image image=&#8221;12376&#8243; img_size=&#8221;full&#8221; alignment=&#8221;center&#8221;][vc_column_text]<span class=\"bildunterschrift\">A 1.5 kb amplicon treated with Exo-CIP Rapid PCR Cleanup Kit prior to sequencing eliminates miscalls and lowers background interference compared to untreated samples. The sequencing results were examined at different locations from the primer binding site. Results seen with Exo-CIP Rapid PCR Cleanup Kit are comparable to ExoSAP-IT PCR Cleanup Reagent.<\/span>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator color=&#8221;custom&#8221; accent_color=&#8221;#dd9933&#8243;][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<\/p>\n<h2>Available products:<\/h2>\n<table style=\"width: 760px;\" border=\"0\" cellpadding=\"5\">\n<tbody>\n<tr style=\"background-color: #f3f3f3; font-size: 16px;\">\n<th style=\"border-bottom: solid #eb7e2d;\" align=\"left\" width=\"400\" height=\"40\">PRODUCT<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"88\">Prod. #<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"100\">SIZE<\/th>\n<th style=\"border-bottom: solid #eb7e2d; background-color: #eb7e2d; color: white;\" colspan=\"3\">PRICE<\/th>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Exo-CIP Rapid PCR Cleanup Kit<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E1050S\/L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 \/ 400 reactions<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/neb-online.fr\/en\/art\/E1050@\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"alignnone\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"http:\/\/international.neb.com\/E1050\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row][vc_column][vc_column_text] Fast and convenient PCR Cleanup [\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]The Exo-CIP Rapid PCR Cleanup Kit is the ideal solution for convenient PCR purification. The Exo-CIP PCR purification kit contains optimized formulations of two enzymes. The thermolabile Exonuclease I digests excess primers while the thermolabile CIP (Calf Intestinal Phosphatase) dephosphorylates excess dNTPs. Both enzymes can be heat-inactivated in&#8230;  <a class=\"excerpt-read-more\" href=\"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/exo-cip-rapid-pcr-cleanup-kit\/\" title=\"Read Exo-CIP Rapid PCR Cleanup Kit\">Read more &raquo;<\/a><\/p>\n","protected":false},"author":15,"featured_media":12373,"parent":2092,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-sidebar-pcr-dna-amplification.php","meta":{"footnotes":""},"class_list":["post-12389","page","type-page","status-publish","has-post-thumbnail","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.0 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Exo-CIP Rapid PCR Cleanup Kit - New England Biolabs France<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/exo-cip-rapid-pcr-cleanup-kit\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Exo-CIP Rapid PCR Cleanup Kit - New England Biolabs France\" \/>\n<meta property=\"og:description\" content=\"[vc_row][vc_column][vc_column_text] Fast and convenient PCR Cleanup [\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]The Exo-CIP Rapid PCR Cleanup Kit is the ideal solution for convenient PCR purification. The Exo-CIP PCR purification kit contains optimized formulations of two enzymes. The thermolabile Exonuclease I digests excess primers while the thermolabile CIP (Calf Intestinal Phosphatase) dephosphorylates excess dNTPs. Both enzymes can be heat-inactivated in... 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