{"id":1524,"date":"2015-04-22T11:32:26","date_gmt":"2015-04-22T11:32:26","guid":{"rendered":"http:\/\/www.neb-online.fr\/?page_id=1524"},"modified":"2022-05-17T08:59:10","modified_gmt":"2022-05-17T06:59:10","slug":"t4-dna-ligase","status":"publish","type":"page","link":"https:\/\/www.neb-online.fr\/en\/t4-dna-ligase\/","title":{"rendered":"NEB DNA Ligase"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row][vc_column][vc_row_inner][vc_column_inner width=&#8221;1\/2&#8243;][vc_column_text]Ligation of DNA is a critical step in many modern molecular biology workflows. The sealing of nicks between adjacent residues of a single-strand break on a double-strand substrate and the joining of double-strand breaks are enzymatically catalyzed by DNA ligases.[\/vc_column_text][\/vc_column_inner][vc_column_inner width=&#8221;1\/2&#8243;][vc_column_text]For the full list of DNA Ligases and Ligase Master Mixes offered by NEB, as well as suggested applications, please use our <a href=\"https:\/\/international.neb.com\/tools-and-resources\/selection-charts\/dna-ligase-selection-chart\" target=\"_blank\" rel=\"noopener noreferrer\">DNA Ligase Selection Chart<\/a>.[\/vc_column_text][vc_single_image image=&#8221;11123&#8243; alignment=&#8221;center&#8221; onclick=&#8221;img_link_large&#8221;][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][vc_row_inner][vc_column_inner width=&#8221;1\/2&#8243;]<div class=\"vbox\">\n\t\t\t\t<span class=\"vhead\">Advantages:<\/span>\n\t\t\t\t\t<ul class=\"vlist\">\n\t\t\t\t\t\t<li><span>Fast ligations<br \/><\/span><\/li><li><span>Robust ligation efficiency<br \/><\/span><\/li><li><span>Industry standard for purity<br \/><\/span><\/li><li><span>Most extensive selection commercially available<br \/><\/span><\/li><li><span>New formulations optimized for your substrates<br \/><\/span><\/li><li><span>Convenient master mix formats<\/span><\/li>\n\t\t\t\t\t<\/ul>\n\t\t\t<\/div>[\/vc_column_inner][vc_column_inner width=&#8221;1\/2&#8243;][vc_single_image image=&#8221;1515&#8243; img_size=&#8221;full&#8221;][vc_column_text]<strong><em>Extreme purity with NEB\u2019s\u00a0<\/em><\/strong><strong><em><u><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/M0202@\">T4 DNA Ligase<\/a><\/u><\/em><\/strong><strong><em><br \/>\n<\/em><\/strong><em>Equivalent amounts of protein were loaded and silver stained using SilverXpress\u2122. Marker M is NEB\u2019s\u00a0<\/em><em><u>Broad Range\u00a0Protein Marker (NEB #P7702)<\/u><\/em><em>.<\/em>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_separator][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<\/p>\n<table class=\"tableborder\" border=\"0\" cellspacing=\"0\" cellpadding=\"2\">\n<tbody>\n<tr id=\"\" dir=\"\" lang=\"\" align=\"\" valign=\"\" bgcolor=\"\">\n<td class=\"tdborder bodycopy\" colspan=\"3\" valign=\"middle\" bgcolor=\"#58595b\">\u00a0<span style=\"color: #ffffff;\">T4 DNA Ligase Quality Control<\/span><\/td>\n<\/tr>\n<tr>\n<td class=\"tdborder bodycopy\" valign=\"middle\">Physical Purity<\/td>\n<td class=\"tdborder bodycopy\" valign=\"middle\">Determined by SDS-PAGE analysis (silver\u00a0stained gel) (Figure 1).<\/td>\n<\/tr>\n<tr>\n<td class=\"tdborder bodycopy\" valign=\"middle\" bgcolor=\"#dcddde\">Exonuclease Activity<\/td>\n<td class=\"tdborder bodycopy\" valign=\"middle\" bgcolor=\"#dcddde\">Contaminating exonucleases are assayed by\u00a0monitoring release of radioactively labeled DNA\u00a0substrate. Very low levels of specific types of\u00a0exonuclease contamination are also assayed for\u00a0using capillary electrophoresis with dye labeled\u00a0substrate (Figure 2).<\/td>\n<\/tr>\n<tr>\n<td class=\"tdborder bodycopy\" valign=\"middle\">Nuclease Activity<\/td>\n<td class=\"tdborder bodycopy\" valign=\"middle\">Contaminating nucleases are assayed\u00a0by incubation with HindIII fragments of\u00a0lambda DNA.<\/td>\n<\/tr>\n<tr>\n<td class=\"tdborder bodycopy\" valign=\"middle\" bgcolor=\"#dcddde\">Endonuclease Activity<\/td>\n<td class=\"tdborder bodycopy\" valign=\"middle\" bgcolor=\"#dcddde\">Incubation with supercoiled plasmid DNA for\u00a04 hours is used to detect any nicking or\u00a0nonspecific nuclease degradation.<\/td>\n<\/tr>\n<tr>\n<td class=\"tdborder bodycopy\" valign=\"middle\">Functional Assay<\/td>\n<td class=\"tdborder bodycopy\" valign=\"middle\">Room temperature ligation is performed.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<\/p>\n<h2>Available Products<\/h2>\n<table style=\"width: 753px; height: 333px;\" border=\"0\" cellpadding=\"5\">\n<tbody>\n<tr style=\"background-color: #f3b281;\">\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"396\" height=\"40\">PRODUCT<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"80\">REF.<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"132\">SIZE<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" colspan=\"1\" width=\"180\">PRICE<\/th>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"2\">Salt-T4 DNA Ligase<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0467 S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">20 000 units<\/td>\n<td style=\"text-align: center; border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/m0467S\"><img decoding=\"async\" class=\"wp-image-4375 size-full aligncenter\" src=\"http:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0467 L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 000 units<\/td>\n<td style=\"text-align: center; border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/m0467L\"><img decoding=\"async\" class=\"wp-image-4375 size-full aligncenter\" src=\"http:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"2\">Hi-T4 DNA Ligase<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M2622 S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">20 000 units<\/td>\n<td style=\"text-align: center; border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/m2622S\"><img decoding=\"async\" class=\"wp-image-4375 size-full aligncenter\" src=\"http:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M2622 L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 000 units<\/td>\n<td style=\"text-align: center; border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/m2622L\"><img decoding=\"async\" class=\"wp-image-4375 size-full aligncenter\" src=\"http:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"2\">T4 DNA Ligase<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0202 S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">20 000 units<\/td>\n<td style=\"text-align: center; border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/m0202S\"><img decoding=\"async\" class=\"wp-image-4375 size-full aligncenter\" src=\"http:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0202 L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 000 units<\/td>\n<td style=\"text-align: center; border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/m0202L\"><img decoding=\"async\" class=\"wp-image-4375 size-full aligncenter\" src=\"http:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"2\">T4 DNA Ligase, conc.<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0202 T<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">20 000 units<\/td>\n<td style=\"text-align: center; border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/m0202T\"><img decoding=\"async\" class=\"wp-image-4375 size-full aligncenter\" src=\"http:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0202 M<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 000 units<\/td>\n<td style=\"text-align: center; border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/m0202M\"><img decoding=\"async\" class=\"wp-image-4375 size-full aligncenter\" src=\"http:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"2\">Quick Ligation\u2122 Kit<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M2200 S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">30 reactions<\/td>\n<td style=\"text-align: center; border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/m2200S\"><img decoding=\"async\" class=\"wp-image-4375 size-full aligncenter\" src=\"http:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M2200 L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">150 reactions<\/td>\n<td style=\"text-align: center; border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/m2200L\"><img decoding=\"async\" class=\"wp-image-4375 size-full aligncenter\" src=\"http:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row][vc_column][vc_row_inner][vc_column_inner width=&#8221;1\/2&#8243;][vc_column_text]Ligation of DNA is a critical step in many modern molecular biology workflows. The sealing of nicks between adjacent residues of a single-strand break on a double-strand substrate and the joining of double-strand breaks are enzymatically catalyzed by DNA ligases.[\/vc_column_text][\/vc_column_inner][vc_column_inner width=&#8221;1\/2&#8243;][vc_column_text]For the full list of DNA Ligases and Ligase Master Mixes offered by NEB,&#8230;  <a class=\"excerpt-read-more\" href=\"https:\/\/www.neb-online.fr\/en\/t4-dna-ligase\/\" title=\"Read NEB DNA Ligase\">Read more &raquo;<\/a><\/p>\n","protected":false},"author":15,"featured_media":1574,"parent":0,"menu_order":0,"comment_status":"open","ping_status":"open","template":"page-sidebar-cloning-synthetic-biology.php","meta":{"footnotes":""},"class_list":["post-1524","page","type-page","status-publish","has-post-thumbnail","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.0 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>NEB DNA Ligase - New England Biolabs France<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.neb-online.fr\/en\/t4-dna-ligase\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"NEB DNA Ligase - New England Biolabs France\" \/>\n<meta property=\"og:description\" content=\"[vc_row][vc_column][vc_row_inner][vc_column_inner width=&#8221;1\/2&#8243;][vc_column_text]Ligation of DNA is a critical step in many modern molecular biology workflows. The sealing of nicks between adjacent residues of a single-strand break on a double-strand substrate and the joining of double-strand breaks are enzymatically catalyzed by DNA ligases.[\/vc_column_text][\/vc_column_inner][vc_column_inner width=&#8221;1\/2&#8243;][vc_column_text]For the full list of DNA Ligases and Ligase Master Mixes offered by NEB,... 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