{"id":15970,"date":"2020-12-18T15:07:03","date_gmt":"2020-12-18T14:07:03","guid":{"rendered":"https:\/\/www.neb-online.fr\/?page_id=15970"},"modified":"2022-07-29T09:18:40","modified_gmt":"2022-07-29T07:18:40","slug":"monarch-hmw-dna-extraction-kits","status":"publish","type":"page","link":"https:\/\/www.neb-online.fr\/en\/monarch-nucleic-acid-purification-kits\/monarch-hmw-dna-extraction-kits\/","title":{"rendered":"Monarch HMW DNA Extraction Kits"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]The <strong><span style=\"color: #f58025;\">Monarch HMW DNA Extraction Kits<\/span><\/strong> provide a rapid and reliable process for extracting high molecular weight DNA (HMW DNA) from biological samples. These kits utilize an optimized process that combines gentle cell lysis with a tunable fragment length generation, followed by precipitation of the extracted DNA onto the surface of large glass beads. DNA size ranges from 50-250 kb for the standard protocol and into the Mb range for certain sample types when the lowest agitation speeds are used. Purified DNA is recovered in high yield with excellent purity, including nearly complete removal of RNA, and is ready for use in downstream applications including long-read sequencing.<\/p>\n<p>[\/vc_column_text][\/vc_column][vc_column width=&#8221;2\/5&#8243;]<div class=\"vbox\">\n\t\t\t\t<span class=\"vhead\">Advantages:<\/span>\n\t\t\t\t\t<ul class=\"vlist\">\n\t\t\t\t\t\t<li><span>Extremely fast, user-friendly protocols utilizing a novel glass-bead-based approach<br \/><\/span><\/li><li><span>Reproducibly purify high molecular weight genomic DNA (HMW DNA) from various sample types<br \/><\/span><\/li><li><span>Tune fragment length by varying agitation speeds during lysis; achieve DNA in the Megabase size range with low speeds<br \/><\/span><\/li><li><span>Achieve outstanding results when compared to other commercially available solutions<br \/><\/span><\/li><li><span>Excellent performance in long read sequencing<\/span><\/li>\n\t\t\t\t\t<\/ul>\n\t\t\t<\/div>[\/vc_column][\/vc_row][vc_row][vc_column][vc_separator color=&#8221;custom&#8221; accent_color=&#8221;#eb7e2d&#8221;][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<\/p>\n<h2><strong>Comparison of HMW DNA Extraction Methods <\/strong><\/h2>\n<img fetchpriority=\"high\" decoding=\"async\" class=\"alignleft size-full wp-image-15947\" src=\"https:\/\/www.neb-online.fr\/wp-content\/uploads\/Monarch_HMW_compare.png\" alt=\"\" width=\"1676\" height=\"944\" srcset=\"https:\/\/www.neb-online.fr\/wp-content\/uploads\/Monarch_HMW_compare.png 1676w, https:\/\/www.neb-online.fr\/wp-content\/uploads\/Monarch_HMW_compare-300x169.png 300w, https:\/\/www.neb-online.fr\/wp-content\/uploads\/Monarch_HMW_compare-1024x577.png 1024w, https:\/\/www.neb-online.fr\/wp-content\/uploads\/Monarch_HMW_compare-768x433.png 768w, https:\/\/www.neb-online.fr\/wp-content\/uploads\/Monarch_HMW_compare-1536x865.png 1536w\" sizes=\"(max-width: 1676px) 100vw, 1676px\" \/>\n<h2>Extremely fast, user-friendly protocols utilizing a novel glass-bead-based approach:<\/h2>\n<p>&nbsp;<\/p>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;2\/3&#8243;][vc_row_inner][vc_column_inner]<img decoding=\"async\" class=\"alignleft size-full wp-image-15953\" src=\"https:\/\/www.neb-online.fr\/wp-content\/uploads\/Monarch_HMW_gDNA_CellProtocol_1219.png\" alt=\"\" width=\"800\" height=\"429\" srcset=\"https:\/\/www.neb-online.fr\/wp-content\/uploads\/Monarch_HMW_gDNA_CellProtocol_1219.png 800w, https:\/\/www.neb-online.fr\/wp-content\/uploads\/Monarch_HMW_gDNA_CellProtocol_1219-300x161.png 300w, https:\/\/www.neb-online.fr\/wp-content\/uploads\/Monarch_HMW_gDNA_CellProtocol_1219-768x412.png 768w\" sizes=\"(max-width: 800px) 100vw, 800px\" \/>[\/vc_column_inner][\/vc_row_inner][\/vc_column][vc_column width=&#8221;1\/3&#8243;]<a href=\"https:\/\/international.neb.com\/tools-and-resources\/video-library?device=modal&amp;videoid={247ab043-164c-412f-9986-1dcbdc6404e6}\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"alignleft wp-image-15955 size-full\" src=\"https:\/\/www.neb-online.fr\/wp-content\/uploads\/Monarch_HMW_Video2020.png\" alt=\"\" width=\"564\" height=\"322\" srcset=\"https:\/\/www.neb-online.fr\/wp-content\/uploads\/Monarch_HMW_Video2020.png 564w, https:\/\/www.neb-online.fr\/wp-content\/uploads\/Monarch_HMW_Video2020-300x171.png 300w\" sizes=\"(max-width: 564px) 100vw, 564px\" \/><\/a>[vc_column_text]<span class=\"bildunterschrift\">Overview of High Molecular Weight DNA Extraction using the Monarch HMW DNA Extraction Kit for Cells &amp; Blood. Visualize how HMW DNA is extracted from cells and blood with the Monarch HMW DNA Extraction Kit for Cells &amp; Blood (NEB #T3050).<br \/>\n<\/span>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator color=&#8221;custom&#8221; accent_color=&#8221;#eb7e2d&#8221;][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<strong>Reproducible Extraction of HMW DNA from Cells and Blood with the Monarch HMW DNA Extraction Kit: <\/strong>[\/vc_column_text]<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-15950\" src=\"https:\/\/www.neb-online.fr\/wp-content\/uploads\/Monarch_HMW_GelExtraction_0220.png\" alt=\"\" width=\"765\" height=\"622\" srcset=\"https:\/\/www.neb-online.fr\/wp-content\/uploads\/Monarch_HMW_GelExtraction_0220.png 765w, https:\/\/www.neb-online.fr\/wp-content\/uploads\/Monarch_HMW_GelExtraction_0220-300x244.png 300w\" sizes=\"(max-width: 765px) 100vw, 765px\" \/>[vc_column_text]<span class=\"bildunterschrift\"><em>DNA extracted with Monarch HMW DNA Extraction Kit for Cells &amp; Blood. 1 x 10<sup>6<\/sup> fresh HEK293 cells and 500 \u03bcl fresh human blood were used as inputs and for preps performed according to the kit instructions using the agitation speed indicated above the gel lanes. 500 ng of DNA from the replicates was resolved by PFGE (1% agarose gel, 6 V\/cm, 13\u00b0C for 20 hours, switch times ramped from 0.5\u201394 seconds on a BioRad<sup>\u00ae<\/sup> CHEF-DR<sup>\u00ae<\/sup> III System). Yield and purity ratios of the individual preps are shown in the accompanying tables. Lambda PFG Ladder (<a href=\"https:\/\/international.neb.com\/n0341\" target=\"_blank\" rel=\"noopener\">NEB #N0341<\/a>) was used as molecular weight standard. <\/em><\/span>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator color=&#8221;custom&#8221; accent_color=&#8221;#eb7e2d&#8221;][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<\/p>\n<h3><strong>The Monarch HMW DNA Extraction Kit for Tissue efficiently purifies high-quality, HMW DNA from a variety of sample types <\/strong>:<\/h3>\n<p>[\/vc_column_text]<img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-15960\" src=\"https:\/\/www.neb-online.fr\/wp-content\/uploads\/Monarch_HMW_SampleTypesGel_0420.png\" alt=\"\" width=\"400\" height=\"771\" srcset=\"https:\/\/www.neb-online.fr\/wp-content\/uploads\/Monarch_HMW_SampleTypesGel_0420.png 400w, https:\/\/www.neb-online.fr\/wp-content\/uploads\/Monarch_HMW_SampleTypesGel_0420-156x300.png 156w\" sizes=\"(max-width: 400px) 100vw, 400px\" \/>[vc_column_text]<span class=\"bildunterschrift\"><em>HMW genomic DNA extracted with the Monarch HMW DNA Extraction Kit for Tissue. 10 mg frozen rat kidney, 10 mg fresh mouse liver, 20 mg frozen mouse brain, 20 mg fresh mouse muscle, 2 x 10<sup>9<\/sup> frozen E. coli cells, 2 x 10<sup>8<\/sup> frozen B. cereus cells, 4 mg fresh X. laevis, 3.8 x 10<sup>8<\/sup> fresh S. cerevisiae cells and 15 mg frozen A. aegypti were used as inputs for preps. Preps were performed according to the kit instructions with sample agitation at 2000 rpm. A modified workflow was used to process S. cerevisiae preps. 500 ng of DNA from each sample prep was resolved by PFGE (1% agarose gel, 6 V\/cm, 13\u00b0C for 20 hours, switch times ramped from 0.5-94 seconds on a BioRad CHEF-DR III System). Yield and purity ratios of the individual preps are shown in the accompanying tables. Lambda PFG Ladder (<a href=\"https:\/\/international.neb.com\/n0341\" target=\"_blank\" rel=\"noopener\">NEB #N0341<\/a>) was used as a molecular weight standard. <\/em><\/span>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator color=&#8221;custom&#8221; accent_color=&#8221;#eb7e2d&#8221;][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<strong> Monarch HMW DNA Extraction Kit produces high molecular weight DNA with excellent yields, purity, and fragment length as compared to other commercially available kits:<\/strong>[\/vc_column_text]<img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-15963\" src=\"https:\/\/www.neb-online.fr\/wp-content\/uploads\/Monarch_HMW_CompetitorGel_0420.png\" alt=\"\" width=\"800\" height=\"786\" srcset=\"https:\/\/www.neb-online.fr\/wp-content\/uploads\/Monarch_HMW_CompetitorGel_0420.png 800w, https:\/\/www.neb-online.fr\/wp-content\/uploads\/Monarch_HMW_CompetitorGel_0420-300x295.png 300w, https:\/\/www.neb-online.fr\/wp-content\/uploads\/Monarch_HMW_CompetitorGel_0420-768x755.png 768w\" sizes=\"(max-width: 800px) 100vw, 800px\" \/>[\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<span class=\"bildunterschrift\">HMW was isolated from 1&#215;10<sup>6<\/sup> HEK293 cells and fresh human blood with the kits indicated in the figure legend. Blood input volumes were used as specified in manufacturers\u2019 protocols (N: 500 \u03bcl, C: 200 \u03bcl, R: 500 \u03bcl, Q: 200 \u03bcl, P: 300 \u03bcl, Z: 200 \u03bcl).<\/span><\/p>\n<p><span class=\"bildunterschrift\">Monarch samples (lanes 1-2) were purified at maximum agitation speed during lysis (2000 rpm). Variation in fragment length of cellular DNA using the standard protocols for Monarch and Circulomics (lanes 1-2 and 3-4, respectively) results from agitation speeds during lysis (Monarch: 2000 rpm, Circulomics: 900 rpm). All other data presented are duplicate samples from each different kit and the standard protocols dedicated to blood or cells were followed. Qiagen does not provide a protocol for cultured cells; a modified version of the blood protocol was followed. Samples were eluted in 100 \u03bcl, except for Zymo which was eluted in 50 \u03bcl according to their recommendations.<\/span><\/p>\n<p><span class=\"bildunterschrift\">Yield and purity of the standard samples were analyzed on Trinean Dropsense 16 spectrophotometer (now Unchained Labs Lunatic. Reported blood sample yields were normalized per 100 \u03bcl. RNA content was determined by HPLC analysis of nucleoside content after digestion of 1 \u03bcg of eluted DNA with the Nucleoside Digestion Mix (<a href=\"https:\/\/international.neb.com\/products\/m0649-nucleoside-digestion-mix\">NEB #M0649<\/a>) . The optional RNase treatment was performed with the Zymo prep. <\/span>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column]<img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-15965\" src=\"https:\/\/www.neb-online.fr\/wp-content\/uploads\/MonarchHMWsingleRunSeqLongRead.png\" alt=\"\" width=\"1646\" height=\"908\" srcset=\"https:\/\/www.neb-online.fr\/wp-content\/uploads\/MonarchHMWsingleRunSeqLongRead.png 1646w, https:\/\/www.neb-online.fr\/wp-content\/uploads\/MonarchHMWsingleRunSeqLongRead-300x165.png 300w, https:\/\/www.neb-online.fr\/wp-content\/uploads\/MonarchHMWsingleRunSeqLongRead-1024x565.png 1024w, https:\/\/www.neb-online.fr\/wp-content\/uploads\/MonarchHMWsingleRunSeqLongRead-768x424.png 768w, https:\/\/www.neb-online.fr\/wp-content\/uploads\/MonarchHMWsingleRunSeqLongRead-1536x847.png 1536w\" sizes=\"(max-width: 1646px) 100vw, 1646px\" \/>[\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<span class=\"bildunterschrift\"><em>DNA used for the sequencing libraries was extracted by following the standard protocols for cultured cells and fresh mammalian blood samples, without further size selection. Mouse kidney DNA samples were extracted from fresh samples using a rotor-stator homogenizer for sample homogenization. Libraries were generated using the LSK109 ligation sequencing kit and loaded on a FLO-MIN106D flow cell. Sequencing was performed on a GridION<sup>\u00ae<\/sup> Mk1 for up to 48 hours, or shorter if no more data was generated by the flow cell. No additional treatment of the flow cell (e.g., flushing) was employed. In the samples that resulted in &gt; 10 Gb of data, 800\u20131,000 ng of DNA library was loaded onto the flow cell for optimal sequencing performance and effective pore usage. Read lengths are indicated in bases unless otherwise noted.<\/em><\/span>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator color=&#8221;custom&#8221; accent_color=&#8221;#eb7e2d&#8221;][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<\/p>\n<h2>Available Monarch HMW DNA Extraction Kits and companion products:<\/h2>\n<table style=\"width: 760px;\" border=\"0\" cellpadding=\"5\">\n<tbody>\n<tr style=\"background-color: #f3f3f3;\">\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"400\" height=\"40px\">PRODUCT<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"88\">Art.No.:<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"100\">SIZE<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" colspan=\"3\">PRICE<\/th>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Monarch HMW DNA Extraction Kit for Cells &amp; Blood<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">T3050S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">5 preps<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/3050@\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/international.neb.com\/T3050\" target=\"blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"NEB Info_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Monarch HMW DNA Extraction Kit for Cells &amp; Blood<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">T3050L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">50 preps<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/3050@\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/international.neb.com\/T3050\" target=\"blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"NEB Info_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Monarch HMW DNA Extraction Kit for Tissue<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">T3060S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">5 preps<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/3060@\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/international.neb.com\/T3060\" target=\"blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"NEB Info_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Monarch HMW DNA Extraction Kit for Tissue<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">T3060L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">50 preps<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/3060@\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/international.neb.com\/T3060\" target=\"blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"NEB Info_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Monarch Pestle Set<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">T3000L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 pieces<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/3000@\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/international.neb.com\/T3000\" target=\"blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"NEB Info_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Monarch 2 ml Tubes<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">T3003L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 pieces<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/3003@\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/international.neb.com\/T3003\" target=\"blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"NEB Info_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Monarch DNA Capture Beads<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">T3005L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">200 pieces<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/3005@\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/international.neb.com\/T3005\" target=\"blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"NEB Info_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Monarch Bead Retainers<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">T3004L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 pieces<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/3004@\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/international.neb.com\/T3004\" target=\"blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"NEB Info_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Monarch gDNA Nuclei Prep &amp; Lysis Buffer Pack<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">T3054L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">1 pack<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/3054@\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/international.neb.com\/T3054\" target=\"blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"NEB Info_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Monarch RBC Lysis Buffer<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">T3051L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">160 ml<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/3051@\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/international.neb.com\/T3051\" target=\"blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"NEB Info_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Monarch gDNA Elution Buffer II<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">T3056L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">24 ml<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/3056@\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/international.neb.com\/T3056\" target=\"blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"NEB Info_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Monarch HMW gDNA Tissue Lysis Buffer<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">T3061L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">62 ml<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/3061@\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/international.neb.com\/T3061\" target=\"blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"NEB Info_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Monarch Protein Separation Solution<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">T3062L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">36 ml<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/3062@\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/international.neb.com\/T3062\" target=\"blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"NEB Info_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Monarch Precipitation Enhancer<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">T3055L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">10 ml<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/3055@\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/international.neb.com\/T3055\" target=\"blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"NEB Info_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]The Monarch HMW DNA Extraction Kits provide a rapid and reliable process for extracting high molecular weight DNA (HMW DNA) from biological samples. These kits utilize an optimized process that combines gentle cell lysis with a tunable fragment length generation, followed by precipitation of the extracted DNA onto the surface of large glass beads&#8230;.  <a class=\"excerpt-read-more\" href=\"https:\/\/www.neb-online.fr\/en\/monarch-nucleic-acid-purification-kits\/monarch-hmw-dna-extraction-kits\/\" title=\"Read Monarch HMW DNA Extraction Kits\">Read more &raquo;<\/a><\/p>\n","protected":false},"author":15,"featured_media":15944,"parent":7186,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-sidebar-nucleic-acid-purification.php","meta":{"footnotes":""},"class_list":["post-15970","page","type-page","status-publish","has-post-thumbnail","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.0 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Monarch HMW DNA Extraction Kits - New England Biolabs France<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.neb-online.fr\/en\/monarch-nucleic-acid-purification-kits\/monarch-hmw-dna-extraction-kits\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Monarch HMW DNA Extraction Kits - New England Biolabs France\" \/>\n<meta property=\"og:description\" content=\"[vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]The Monarch HMW DNA Extraction Kits provide a rapid and reliable process for extracting high molecular weight DNA (HMW DNA) from biological samples. These kits utilize an optimized process that combines gentle cell lysis with a tunable fragment length generation, followed by precipitation of the extracted DNA onto the surface of large glass beads.... Read more &raquo;\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.neb-online.fr\/en\/monarch-nucleic-acid-purification-kits\/monarch-hmw-dna-extraction-kits\/\" \/>\n<meta property=\"og:site_name\" content=\"New England Biolabs France\" \/>\n<meta property=\"article:modified_time\" content=\"2022-07-29T07:18:40+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.neb-online.fr\/wp-content\/uploads\/Homebanner_Monarch_HMW_EN3.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1080\" \/>\n\t<meta property=\"og:image:height\" content=\"284\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"13 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.neb-online.fr\/en\/monarch-nucleic-acid-purification-kits\/monarch-hmw-dna-extraction-kits\/\",\"url\":\"https:\/\/www.neb-online.fr\/en\/monarch-nucleic-acid-purification-kits\/monarch-hmw-dna-extraction-kits\/\",\"name\":\"Monarch HMW DNA Extraction Kits - New England Biolabs France\",\"isPartOf\":{\"@id\":\"https:\/\/www.neb-online.fr\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\/\/www.neb-online.fr\/en\/monarch-nucleic-acid-purification-kits\/monarch-hmw-dna-extraction-kits\/#primaryimage\"},\"image\":{\"@id\":\"https:\/\/www.neb-online.fr\/en\/monarch-nucleic-acid-purification-kits\/monarch-hmw-dna-extraction-kits\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/www.neb-online.fr\/wp-content\/uploads\/Homebanner_Monarch_HMW_EN3.jpg\",\"datePublished\":\"2020-12-18T14:07:03+00:00\",\"dateModified\":\"2022-07-29T07:18:40+00:00\",\"breadcrumb\":{\"@id\":\"https:\/\/www.neb-online.fr\/en\/monarch-nucleic-acid-purification-kits\/monarch-hmw-dna-extraction-kits\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/www.neb-online.fr\/en\/monarch-nucleic-acid-purification-kits\/monarch-hmw-dna-extraction-kits\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/www.neb-online.fr\/en\/monarch-nucleic-acid-purification-kits\/monarch-hmw-dna-extraction-kits\/#primaryimage\",\"url\":\"https:\/\/www.neb-online.fr\/wp-content\/uploads\/Homebanner_Monarch_HMW_EN3.jpg\",\"contentUrl\":\"https:\/\/www.neb-online.fr\/wp-content\/uploads\/Homebanner_Monarch_HMW_EN3.jpg\",\"width\":1080,\"height\":284},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/www.neb-online.fr\/en\/monarch-nucleic-acid-purification-kits\/monarch-hmw-dna-extraction-kits\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Monarch Kits &#8211; Nucleic Acid Purification\",\"item\":\"https:\/\/www.neb-online.fr\/en\/monarch-nucleic-acid-purification-kits\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Monarch HMW DNA Extraction Kits\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/www.neb-online.fr\/#website\",\"url\":\"https:\/\/www.neb-online.fr\/\",\"name\":\"New England Biolabs France\",\"description\":\"be INSPIRED, drive DISCOVERY, stay GENUINE\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/www.neb-online.fr\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Monarch HMW DNA Extraction Kits - New England Biolabs France","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.neb-online.fr\/en\/monarch-nucleic-acid-purification-kits\/monarch-hmw-dna-extraction-kits\/","og_locale":"en_US","og_type":"article","og_title":"Monarch HMW DNA Extraction Kits - New England Biolabs France","og_description":"[vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]The Monarch HMW DNA Extraction Kits provide a rapid and reliable process for extracting high molecular weight DNA (HMW DNA) from biological samples. These kits utilize an optimized process that combines gentle cell lysis with a tunable fragment length generation, followed by precipitation of the extracted DNA onto the surface of large glass beads.... Read more &raquo;","og_url":"https:\/\/www.neb-online.fr\/en\/monarch-nucleic-acid-purification-kits\/monarch-hmw-dna-extraction-kits\/","og_site_name":"New England Biolabs France","article_modified_time":"2022-07-29T07:18:40+00:00","og_image":[{"width":1080,"height":284,"url":"https:\/\/www.neb-online.fr\/wp-content\/uploads\/Homebanner_Monarch_HMW_EN3.jpg","type":"image\/jpeg"}],"twitter_misc":{"Est. reading time":"13 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/www.neb-online.fr\/en\/monarch-nucleic-acid-purification-kits\/monarch-hmw-dna-extraction-kits\/","url":"https:\/\/www.neb-online.fr\/en\/monarch-nucleic-acid-purification-kits\/monarch-hmw-dna-extraction-kits\/","name":"Monarch HMW DNA Extraction Kits - New England Biolabs France","isPartOf":{"@id":"https:\/\/www.neb-online.fr\/#website"},"primaryImageOfPage":{"@id":"https:\/\/www.neb-online.fr\/en\/monarch-nucleic-acid-purification-kits\/monarch-hmw-dna-extraction-kits\/#primaryimage"},"image":{"@id":"https:\/\/www.neb-online.fr\/en\/monarch-nucleic-acid-purification-kits\/monarch-hmw-dna-extraction-kits\/#primaryimage"},"thumbnailUrl":"https:\/\/www.neb-online.fr\/wp-content\/uploads\/Homebanner_Monarch_HMW_EN3.jpg","datePublished":"2020-12-18T14:07:03+00:00","dateModified":"2022-07-29T07:18:40+00:00","breadcrumb":{"@id":"https:\/\/www.neb-online.fr\/en\/monarch-nucleic-acid-purification-kits\/monarch-hmw-dna-extraction-kits\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/www.neb-online.fr\/en\/monarch-nucleic-acid-purification-kits\/monarch-hmw-dna-extraction-kits\/"]}]},{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/www.neb-online.fr\/en\/monarch-nucleic-acid-purification-kits\/monarch-hmw-dna-extraction-kits\/#primaryimage","url":"https:\/\/www.neb-online.fr\/wp-content\/uploads\/Homebanner_Monarch_HMW_EN3.jpg","contentUrl":"https:\/\/www.neb-online.fr\/wp-content\/uploads\/Homebanner_Monarch_HMW_EN3.jpg","width":1080,"height":284},{"@type":"BreadcrumbList","@id":"https:\/\/www.neb-online.fr\/en\/monarch-nucleic-acid-purification-kits\/monarch-hmw-dna-extraction-kits\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Monarch Kits &#8211; Nucleic Acid Purification","item":"https:\/\/www.neb-online.fr\/en\/monarch-nucleic-acid-purification-kits\/"},{"@type":"ListItem","position":2,"name":"Monarch HMW DNA Extraction Kits"}]},{"@type":"WebSite","@id":"https:\/\/www.neb-online.fr\/#website","url":"https:\/\/www.neb-online.fr\/","name":"New England Biolabs France","description":"be INSPIRED, drive DISCOVERY, stay GENUINE","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/www.neb-online.fr\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"}]}},"_links":{"self":[{"href":"https:\/\/www.neb-online.fr\/en\/wp-json\/wp\/v2\/pages\/15970","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.neb-online.fr\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.neb-online.fr\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.neb-online.fr\/en\/wp-json\/wp\/v2\/users\/15"}],"replies":[{"embeddable":true,"href":"https:\/\/www.neb-online.fr\/en\/wp-json\/wp\/v2\/comments?post=15970"}],"version-history":[{"count":5,"href":"https:\/\/www.neb-online.fr\/en\/wp-json\/wp\/v2\/pages\/15970\/revisions"}],"predecessor-version":[{"id":24009,"href":"https:\/\/www.neb-online.fr\/en\/wp-json\/wp\/v2\/pages\/15970\/revisions\/24009"}],"up":[{"embeddable":true,"href":"https:\/\/www.neb-online.fr\/en\/wp-json\/wp\/v2\/pages\/7186"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.neb-online.fr\/en\/wp-json\/wp\/v2\/media\/15944"}],"wp:attachment":[{"href":"https:\/\/www.neb-online.fr\/en\/wp-json\/wp\/v2\/media?parent=15970"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}