{"id":1715,"date":"2015-04-28T07:59:23","date_gmt":"2015-04-28T07:59:23","guid":{"rendered":"http:\/\/www.neb-online.fr\/?page_id=1715"},"modified":"2020-04-16T23:09:56","modified_gmt":"2020-04-16T21:09:56","slug":"nebnext-microbiome-dna-enrichment-kit","status":"publish","type":"page","link":"https:\/\/www.neb-online.fr\/en\/next-generation-sequencing\/nebnext-microbiome-dna-enrichment-kit\/","title":{"rendered":"NEBNext Microbiome DNA Enrichment Kit"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]Microbiome DNA analysis can be challenging due to the high percentage of host DNA present in many samples. The <a href=\"https:\/\/shop.neb-online.fr\/en\/art\/E2612@\" target=\"_blank\" rel=\"noopener noreferrer\">NEBNext Microbiome DNA Enrichment Kit<\/a> facilitates enrichment of microbial genomic DNA from samples contain- ing methylated host DNA (including human), by selective binding and removal of the CpG-methylated host DNA. Importantly, microbial diversity remains intact after enrichment.[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/2&#8243;]<div class=\"vbox\">\n\t\t\t\t<span class=\"vhead\">Advantages:<\/span>\n\t\t\t\t\t<ul class=\"vlist\">\n\t\t\t\t\t\t<li><span>Effective enrichment of microbial genomic DNA from samples contaminating host DNA<br \/><\/span><\/li><li><span>Fast, simple protocol<br \/><\/span><\/li><li><span>Enables microbiome whole genome sequencing, even for samples with high levels of host DNA<br \/><\/span><\/li><li><span>Compatible with downstream applications including next generation sequencing on all platforms, qPCR and end point PCR<br \/><\/span><\/li><li><span>Suitable for a wide range of sample types<br \/><\/span><\/li><li><span>No requirement for live cells<\/span><\/li>\n\t\t\t\t\t<\/ul>\n\t\t\t<\/div>[\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]<strong>Workflow:<\/strong><\/p>\n<ul>\n<li>Prepare Genomic DNA DNA should be free of proteins, proteinase A, SDS and organic solvents; size should be \u2265 15 kb for\u00a0optimal performance.<\/li>\n<li>Combine MBD2-Fc and Magnetic Beads in 1X Bind\/wash Buffer.\u00a0Incubate the reaction for 10 minutes at room temperature.\u00a0Wash beads two times in Bind\/wash Reaction Buffer.<\/li>\n<li>Add DNA to MBD2-Fc Magnetic Beads. Incubate the reaction\u00a0for 15 minutes at room temperature with gentle mixing.<\/li>\n<li>Collect Supernatant Fraction containing enriched microbial\u00a0DNA and Bead Fraction-Containing Host DNA.<\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/2&#8243;][vc_single_image image=&#8221;1113&#8243; img_size=&#8221;full&#8221;][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator color=&#8221;custom&#8221; accent_color=&#8221;#919191&#8243;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]<\/p>\n<h2><strong>Salivary Microbiome DNA Enrichment<\/strong><\/h2>\n<p>DNA was purified from pooled human saliva DNA (Innovative Research) and enriched using the NEBNext Microbiome DNA Enrichment Kit. Libraries were prepared from unenriched and enriched samples and sequenced on the SOLiD 4 platform. The graph shows percentages of 500M-537M SOLiD4 50bp reads that mapped to either the Human reference sequence (hg19) or to a microbe listed in Human Oral Microbiome Database (HOMD)[1]. (Because the HOMD collection is not comprehensive, ~80% of reads in the enriched samples do not map to either database.) Reads were mapped using Bowtie 0.12.7[2] with typical settings (2 mismatches in a 28 bp seed region, etc.).[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/2&#8243;][vc_single_image image=&#8221;1115&#8243; img_size=&#8221;full&#8221;][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator color=&#8221;custom&#8221; accent_color=&#8221;#919191&#8243;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]<\/p>\n<h2><strong>Microbiome Diversity<\/strong><\/h2>\n<p>Microbiome Diversity is Retained after Enrichment with the NEBNext Microbiome DNA Enrichment Kit DNA was purified from pooled human saliva DNA (Innovative Research) and enriched using the NEBNext Microbiome DNA Enrichment Kit. Libraries were prepared from unenriched and enriched samples, followed by sequencing on the SOLiD 4 platform. The graph shows a comparison between relative abundance of each bacterial species listed in HOMD[1] before and after enrichment with the NEBNext Microbiome DNA Enrichment Kit. Abundance is inferred from the number of reads mapping to each species as a percentage of all reads mapping to HOMD. High concordance continues even to very low abundance species (inset). We compared 501M 50bp SOliD4 reads in the enriched dataset to 537M 50bp SOLiD4 reads in the unenriched dataset. Reads were mapped using Bowtie 0.12.7[2] with typical settings (2 mismatches in a 28bp seed region, etc). * Niesseria flavescens \u2013 This organism may have unusual methylation density, allowing it to bind the enriching beads at a low level. Other Niesseria species (N. mucosa, N. sicca and N. elognata) are represented, but do not exhibit this anomalous enrichment.[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/2&#8243;][vc_single_image image=&#8221;1112&#8243; img_size=&#8221;medium&#8221; img_link_large=&#8221;yes&#8221;][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<\/p>\n<h2>Available product<\/h2>\n<table style=\"width: 760px;\" border=\"0\" cellpadding=\"5\">\n<tbody>\n<tr style=\"background-color: #f3b281;\">\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"396\" height=\"40px\">PRODUCT<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"80\">REF.<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"132\">SIZE<\/th>\n<th class=\"\" style=\"border-bottom: solid #eb7e2d;\" colspan=\"1\" width=\"180\">PRICE<\/th>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">NEBNext<sup>\u00ae<\/sup> Microbiome DNA Enrichment Kit<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E2612 S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">6 rxns<\/td>\n<td class=\"\" style=\"text-align: center; border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/e2612s\"><img decoding=\"async\" class=\"wp-image-4375 size-full aligncenter\" src=\"https:\/\/www.neb-online.fr\/\/\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]Microbiome DNA analysis can be challenging due to the high percentage of host DNA present in many samples. The NEBNext Microbiome DNA Enrichment Kit facilitates enrichment of microbial genomic DNA from samples contain- ing methylated host DNA (including human), by selective binding and removal of the CpG-methylated host DNA. Importantly, microbial diversity remains intact&#8230;  <a class=\"excerpt-read-more\" href=\"https:\/\/www.neb-online.fr\/en\/next-generation-sequencing\/nebnext-microbiome-dna-enrichment-kit\/\" title=\"Read NEBNext Microbiome DNA Enrichment Kit\">Read more &raquo;<\/a><\/p>\n","protected":false},"author":15,"featured_media":5295,"parent":2170,"menu_order":0,"comment_status":"open","ping_status":"open","template":"page-sidebar-next-generation-sequencing.php","meta":{"footnotes":""},"class_list":["post-1715","page","type-page","status-publish","has-post-thumbnail","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.0 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>NEBNext Microbiome DNA Enrichment Kit - New England Biolabs France<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.neb-online.fr\/en\/next-generation-sequencing\/nebnext-microbiome-dna-enrichment-kit\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"NEBNext Microbiome DNA Enrichment Kit - New England Biolabs France\" \/>\n<meta property=\"og:description\" content=\"[vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]Microbiome DNA analysis can be challenging due to the high percentage of host DNA present in many samples. The NEBNext Microbiome DNA Enrichment Kit facilitates enrichment of microbial genomic DNA from samples contain- ing methylated host DNA (including human), by selective binding and removal of the CpG-methylated host DNA. Importantly, microbial diversity remains intact... Read more &raquo;\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.neb-online.fr\/en\/next-generation-sequencing\/nebnext-microbiome-dna-enrichment-kit\/\" \/>\n<meta property=\"og:site_name\" content=\"New England Biolabs France\" \/>\n<meta property=\"article:modified_time\" content=\"2020-04-16T21:09:56+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/04\/NEBNext_Banner_Microbiome_760x200px.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"760\" \/>\n\t<meta property=\"og:image:height\" content=\"214\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"3 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.neb-online.fr\/en\/next-generation-sequencing\/nebnext-microbiome-dna-enrichment-kit\/\",\"url\":\"https:\/\/www.neb-online.fr\/en\/next-generation-sequencing\/nebnext-microbiome-dna-enrichment-kit\/\",\"name\":\"NEBNext Microbiome DNA Enrichment Kit - New England Biolabs France\",\"isPartOf\":{\"@id\":\"https:\/\/www.neb-online.fr\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\/\/www.neb-online.fr\/en\/next-generation-sequencing\/nebnext-microbiome-dna-enrichment-kit\/#primaryimage\"},\"image\":{\"@id\":\"https:\/\/www.neb-online.fr\/en\/next-generation-sequencing\/nebnext-microbiome-dna-enrichment-kit\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/04\/NEBNext_Banner_Microbiome_760x200px.jpg\",\"datePublished\":\"2015-04-28T07:59:23+00:00\",\"dateModified\":\"2020-04-16T21:09:56+00:00\",\"breadcrumb\":{\"@id\":\"https:\/\/www.neb-online.fr\/en\/next-generation-sequencing\/nebnext-microbiome-dna-enrichment-kit\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/www.neb-online.fr\/en\/next-generation-sequencing\/nebnext-microbiome-dna-enrichment-kit\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/www.neb-online.fr\/en\/next-generation-sequencing\/nebnext-microbiome-dna-enrichment-kit\/#primaryimage\",\"url\":\"https:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/04\/NEBNext_Banner_Microbiome_760x200px.jpg\",\"contentUrl\":\"https:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/04\/NEBNext_Banner_Microbiome_760x200px.jpg\",\"width\":760,\"height\":214,\"caption\":\"NEBNext Microbiome\"},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/www.neb-online.fr\/en\/next-generation-sequencing\/nebnext-microbiome-dna-enrichment-kit\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Next Generation Sequencing\",\"item\":\"https:\/\/www.neb-online.fr\/en\/next-generation-sequencing\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"NEBNext Microbiome DNA Enrichment Kit\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/www.neb-online.fr\/#website\",\"url\":\"https:\/\/www.neb-online.fr\/\",\"name\":\"New England Biolabs France\",\"description\":\"be INSPIRED, drive DISCOVERY, stay GENUINE\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/www.neb-online.fr\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"NEBNext Microbiome DNA Enrichment Kit - New England Biolabs France","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.neb-online.fr\/en\/next-generation-sequencing\/nebnext-microbiome-dna-enrichment-kit\/","og_locale":"en_US","og_type":"article","og_title":"NEBNext Microbiome DNA Enrichment Kit - New England Biolabs France","og_description":"[vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]Microbiome DNA analysis can be challenging due to the high percentage of host DNA present in many samples. The NEBNext Microbiome DNA Enrichment Kit facilitates enrichment of microbial genomic DNA from samples contain- ing methylated host DNA (including human), by selective binding and removal of the CpG-methylated host DNA. Importantly, microbial diversity remains intact... 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