{"id":1759,"date":"2015-04-29T08:33:30","date_gmt":"2015-04-29T08:33:30","guid":{"rendered":"http:\/\/www.neb-online.fr\/?page_id=1759"},"modified":"2022-11-14T12:47:47","modified_gmt":"2022-11-14T11:47:47","slug":"restriction-enzymes-for-epigenetics","status":"publish","type":"page","link":"https:\/\/www.neb-online.fr\/en\/epigenetics\/restriction-enzymes-for-epigenetics\/","title":{"rendered":"Restriction Enzymes for Epigenetics"},"content":{"rendered":"

[vc_row][vc_column width=”1\/1″][vc_column_text]Many restriction enzymes are sensitive to the DNA methylation states. Cleavage can be blocked or impaired when a particular base in the recognition site is modified.<\/p>\n

Scientists at NEB recently identified the MspJI<\/a> family of restriction enzymes, which are dependent on methylation and hydroxymethylation for cleavage to occur. These enzymes excise ~ 32 base pair fragments containing a centrally located 5-hmC or 5-mC modified residue that can be extracted and sequenced. Due to the known position of this epigenetic modification, bisulfite conversion<\/a> is not required prior to downstream analysis.[\/vc_column_text][vc_row_inner][vc_column_inner width=”1\/2″][vc_column_text]These EpiMark validated, methylation-dependent restriction enzymes expand the potential for mapping epigenetic modifications and simplify the study of DNA methylation. Additionally, they provide an opportunity to better understand the role of 5-hydroxymethylcytosine<\/a> in the genome.<\/p>\n

A variety of our existing restriction enzymes can also be used to study epigenetic modifications of DNA, such as, DpnI<\/a> and DpnII<\/a> that recognize the same sequence, but different methylation patterns. McrBC<\/a> also only cleaves DNA that is methylated on one or both strands.[\/vc_column_text][\/vc_column_inner][vc_column_inner width=”1\/2″][vc_column_text]Advantages:<\/strong><\/p>\n