{"id":1885,"date":"2015-04-24T11:05:36","date_gmt":"2015-04-24T11:05:36","guid":{"rendered":"http:\/\/www.neb-online.fr\/?page_id=1885"},"modified":"2022-11-14T14:38:01","modified_gmt":"2022-11-14T13:38:01","slug":"ligase-master-mixes","status":"publish","type":"page","link":"https:\/\/www.neb-online.fr\/en\/ligase-master-mixes\/","title":{"rendered":"Ligase Master Mixes"},"content":{"rendered":"

[vc_row][vc_column width=”2\/3″ css=”.vc_custom_1429211094416{margin-bottom: 35px !important;}”][vc_column_text]<\/p>\n

Blunt\/TA Ligase Master Mix<\/h3>\n

The Blunt\/TA Ligase Master Mix (NEB #M0367<\/a>) is specifically formulated to improve ligation and transformation of both blunt-end and single-base overhang substrates. This master mix format simplifies reaction setup, ensuring robust, rapid ligation with an optimized ratio of enzyme and buffer components. Reactions can be used to directly transform many strains of chemically competent E. coli<\/em> <\/a>without dilution.[\/vc_column_text][vc_column_text]Blunt\/TA Ligase Master Mix Outperforms the Competition:<\/strong><\/p>\n\"duplicate_ligation_reactions2\"\n

Duplicate ligation reactions of blunt or T\/A vector\/insert pairs were set upaccording to the master mix vendors’ suggestions. Equal amounts of ligated DNA were used to transform NEB 10-beta Competent E. coli (NEB #C3019<\/a>) and triplicate plating was performed. Transformation results were averaged and graphed as a percentage of the highest performing reaction, T\/A ligation using the Blunt\/TA Ligase Master Mix<\/a>.<\/em><\/span>[\/vc_column_text][vc_column_text]<\/p>\n

Instant Sticky-end Ligase Master Mix<\/h3>\n

Complete with T4 DNA Ligase (NEB #M0202<\/a>), proprietary ligation enhancer and optimized reaction buffer, the Instant Sticky-end Ligase Master Mix (NEB #M0370<\/a>) enables instant, incubation- free ligation. Specifically formulated to rapidly ligate sticky-end (2\u20134 bp) substrates and improve transformation, this mix simplifies reaction set-up and reduces the time needed for routine ligations. Reactions can be used to directly transform many strains of chemically competent E. coli without dilution.[\/vc_column_text][vc_column_text]Instant Sticky-end Ligase Master Mix Offers Robust Ligation with No Incubation Step<\/strong><\/p>\n\"\"\n

Reactions containing 20 ng of pUC19 digested with SacI\/SphI, and a 3-foldmolar excess of a 1.6 kb fragment from a different plasmid with compatible ends were set up using the Instant Sticky-end Ligase Master Mix. Without any additional incubation time, 2 \u00b5l were immediately withdrawn and used to transform a 50 \u00b5l aliquot of NEB 10-beta Competent E. coli (NEB #C3019<\/a>). A 50 \u00b5l aliquot of the 1 ml outgrowth was plated onto a selective plate and incubated overnight at 37\u00b0C. Results show that several hundred colonies wereproduced.<\/em><\/span>[\/vc_column_text][\/vc_column][vc_column width=”1\/3″][vc_column_text css=”.vc_custom_1429210895655{margin-bottom: 5px !important;}”]Why use PEG in a Ligation?<\/strong><\/a>[\/vc_column_text][vc_video link=”http:\/\/youtu.be\/S0b0c7wGs68″][vc_column_text css=”.vc_custom_1429210908788{margin-bottom: 5px !important;}”]What is a difficult Ligation?<\/strong><\/a>[\/vc_column_text][vc_video link=”http:\/\/youtu.be\/tJP8NMZX8FE”][vc_column_text css=”.vc_custom_1429210915757{margin-bottom: 5px !important;}”]What are the best reaction times and temperatures?<\/strong><\/a>[\/vc_column_text][vc_video link=”http:\/\/youtu.be\/pe4CLtlj-TU”][vc_column_text css=”.vc_custom_1429210921959{margin-bottom: 5px !important;}”]What are the best ratios and reactants?<\/strong><\/a>[\/vc_column_text][vc_video link=”http:\/\/youtu.be\/z5P1hoWic-8″][vc_column_text css=”.vc_custom_1429210930814{margin-bottom: 5px !important;}”]Which Ligase should I choose?<\/strong><\/a>[\/vc_column_text][vc_video link=”http:\/\/youtu.be\/DD8by7fWhUQ”][vc_separator color=”custom” accent_color=”#919191″][vc_column_text]Datacards:
\n<\/strong>Blunt\/TA Ligase Master Mix
\n<\/strong><\/a>Instant Sticky-End Master Mix<\/strong><\/a>[\/vc_column_text][vc_separator color=”custom” accent_color=”#919191″][vc_column_text]<\/p>\n

Technical Reference Quick Links:
\n<\/strong>Tips for maximizing Ligation Efficiences
\n<\/strong><\/a>Ligation troubleshooting Tips
\n<\/strong><\/a>Enhancing transformation Efficiencies
\n<\/strong><\/a>Competent E. coli<\/em> strains Overview<\/strong><\/a><\/p>\n

[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_text_separator title=”P R O D U C T T A B L E”][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
PRODUCT<\/th>\nProd.Nr.:<\/th>\nSIZE<\/th>\nPRICE<\/th>\n<\/tr>\n
Blunt\/TA Ligase Master Mix<\/td>\nM0367 S<\/td>\n50 reactions<\/td>\n\"shop_icon\"<\/a><\/td>\n<\/tr>\n
M0367 L<\/td>\n250 reactions<\/td>\n\"shop_icon\"<\/a><\/td>\n<\/tr>\n
Instant Sticky-end Master Mix<\/td>\nM0370 S<\/td>\n50 reactions<\/td>\n\"shop_icon\"<\/a><\/td>\n<\/tr>\n
M0370 L<\/td>\n250 reactions<\/td>\n\"shop_icon\"<\/a><\/td>\n<\/tr>\n
ElectroLigase\u2122<\/td>\nM0369 S<\/td>\n50 reactions<\/td>\n\"shop_icon\"<\/a><\/td>\n<\/tr>\n
T4 DNA Ligase<\/td>\nM0202 S<\/td>\n20.000 units<\/td>\n\"shop_icon\"<\/a><\/td>\n<\/tr>\n
M0202 L<\/td>\n100.000 units<\/td>\n\"shop_icon\"<\/a><\/td>\n<\/tr>\n
Quick Ligation Kit<\/td>\nM2200 S<\/td>\n30 reactions<\/td>\n\"shop_icon\"<\/a><\/td>\n<\/tr>\n
M2200 L<\/td>\n150 reactions<\/td>\n\"shop_icon\"<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n
PRODUCT<\/th>\nProd.Nr.:<\/th>\nSIZE<\/th>\nPRICE<\/th>\n<\/tr>\n
T3 DNA Ligase<\/td>\nM0317 S<\/td>\n100.000 units<\/td>\n\"shop_icon\"<\/a><\/td>\n<\/tr>\n
M0317 L<\/td>\n750.000 units<\/td>\n\"shop_icon\"<\/a><\/td>\n<\/tr>\n
T7 DNA Ligase<\/td>\nM0318 S<\/td>\n100.000 units<\/td>\n\"shop_icon\"<\/a><\/td>\n<\/tr>\n
M0318 L<\/td>\n750.000 units<\/td>\n\"shop_icon\"<\/a><\/td>\n<\/tr>\n
E. coli DNA Ligase<\/td>\nM0205 S<\/td>\n200 units<\/td>\n\"shop_icon\"<\/a><\/td>\n<\/tr>\n
M0205 L<\/td>\n1.000 units<\/td>\n\"shop_icon\"<\/a><\/td>\n<\/tr>\n
Taq DNA Ligase<\/td>\nM0208 S<\/td>\n2.000 units<\/td>\n\"shop_icon\"<\/a><\/td>\n<\/tr>\n
M0208 L<\/td>\n10.000 units<\/td>\n\"shop_icon\"<\/a><\/td>\n<\/tr>\n
9\u00b0N\u2122 DNA Ligase<\/td>\nM0238 S<\/td>\n2.500 units<\/td>\n\"shop_icon\"<\/a><\/td>\n<\/tr>\n
M0238 L<\/td>\n12.500 units<\/td>\n\"shop_icon\"<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

The Ligation Module for Library Preparation in Next-Gen-Sequencing<\/strong><\/p>\n\n\n\n\n\n
PRODUCT<\/th>\nProd.Nr.:<\/th>\nSIZE<\/th>\nPRICE<\/th>\n<\/tr>\n
Quick Ligation Module<\/td>\nE6056 S<\/td>\n20 reactions<\/td>\n\"shop_icon\"<\/a><\/td>\n<\/tr>\n
E6056 L<\/td>\n100 reactions<\/td>\n\"shop_icon\"<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"

[vc_row][vc_column width=”2\/3″ css=”.vc_custom_1429211094416{margin-bottom: 35px !important;}”][vc_column_text] Blunt\/TA Ligase Master Mix The Blunt\/TA Ligase Master Mix (NEB #M0367) is specifically formulated to improve ligation and transformation of both blunt-end and single-base overhang substrates. This master mix format simplifies reaction setup, ensuring robust, rapid ligation with an optimized ratio of enzyme and buffer components. Reactions can be used… Read more »<\/a><\/p>\n","protected":false},"author":15,"featured_media":1574,"parent":0,"menu_order":0,"comment_status":"open","ping_status":"open","template":"page-sidebar-cloning-synthetic-biology.php","meta":{"footnotes":""},"class_list":["post-1885","page","type-page","status-publish","has-post-thumbnail","hentry"],"yoast_head":"\nLigase Master Mixes - New England Biolabs France<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.neb-online.fr\/en\/ligase-master-mixes\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Ligase Master Mixes - New England Biolabs France\" \/>\n<meta property=\"og:description\" content=\"[vc_row][vc_column width=”2\/3″ css=”.vc_custom_1429211094416{margin-bottom: 35px !important;}”][vc_column_text] Blunt\/TA Ligase Master Mix The Blunt\/TA Ligase Master Mix (NEB #M0367) is specifically formulated to improve ligation and transformation of both blunt-end and single-base overhang substrates. This master mix format simplifies reaction setup, ensuring robust, rapid ligation with an optimized ratio of enzyme and buffer components. Reactions can be used... Read more »\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.neb-online.fr\/en\/ligase-master-mixes\/\" \/>\n<meta property=\"og:site_name\" content=\"New England Biolabs France\" \/>\n<meta property=\"article:modified_time\" content=\"2022-11-14T13:38:01+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/04\/NEB_Klonierung_ligasesx760px.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"760\" \/>\n\t<meta property=\"og:image:height\" content=\"200\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"8 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.neb-online.fr\/en\/ligase-master-mixes\/\",\"url\":\"https:\/\/www.neb-online.fr\/en\/ligase-master-mixes\/\",\"name\":\"Ligase Master Mixes - New England Biolabs France\",\"isPartOf\":{\"@id\":\"https:\/\/www.neb-online.fr\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\/\/www.neb-online.fr\/en\/ligase-master-mixes\/#primaryimage\"},\"image\":{\"@id\":\"https:\/\/www.neb-online.fr\/en\/ligase-master-mixes\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/04\/NEB_Klonierung_ligasesx760px.jpg\",\"datePublished\":\"2015-04-24T11:05:36+00:00\",\"dateModified\":\"2022-11-14T13:38:01+00:00\",\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/www.neb-online.fr\/en\/ligase-master-mixes\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/www.neb-online.fr\/en\/ligase-master-mixes\/#primaryimage\",\"url\":\"https:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/04\/NEB_Klonierung_ligasesx760px.jpg\",\"contentUrl\":\"https:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/04\/NEB_Klonierung_ligasesx760px.jpg\",\"width\":760,\"height\":200,\"caption\":\"NEB_Ligase_Header\"},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/www.neb-online.fr\/#website\",\"url\":\"https:\/\/www.neb-online.fr\/\",\"name\":\"New England Biolabs France\",\"description\":\"be INSPIRED, drive DISCOVERY, stay GENUINE\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/www.neb-online.fr\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Ligase Master Mixes - New England Biolabs France","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.neb-online.fr\/en\/ligase-master-mixes\/","og_locale":"en_US","og_type":"article","og_title":"Ligase Master Mixes - New England Biolabs France","og_description":"[vc_row][vc_column width=”2\/3″ css=”.vc_custom_1429211094416{margin-bottom: 35px !important;}”][vc_column_text] Blunt\/TA Ligase Master Mix The Blunt\/TA Ligase Master Mix (NEB #M0367) is specifically formulated to improve ligation and transformation of both blunt-end and single-base overhang substrates. 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