{"id":2095,"date":"2015-04-27T12:33:35","date_gmt":"2015-04-27T12:33:35","guid":{"rendered":"http:\/\/www.neb-online.fr\/?page_id=2095"},"modified":"2022-07-28T15:44:54","modified_gmt":"2022-07-28T13:44:54","slug":"onetaq-dna-polymerase","status":"publish","type":"page","link":"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/routine-pcr-en\/onetaq-dna-polymerase\/","title":{"rendered":"OneTaq DNA Polymerase"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row][vc_column][vc_basic_grid post_type=&#8221;post&#8221; max_items=&#8221;10&#8243; element_width=&#8221;12&#8243; gap=&#8221;15&#8243; item=&#8221;4123&#8243; taxonomies=&#8221;74&#8243; grid_id=&#8221;vc_gid:1659014994409-8b82fd8a348ad64fa5743b514bcaea23-10&#8243;][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text][\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;2\/3&#8243; css=&#8221;.vc_custom_1429224286633{margin-bottom: 25px !important;}&#8221;][vc_column_text]<\/p>\n<p class=\"\"><a class=\"\" href=\"https:\/\/www.neb-online.fr\/en\/art\/M0480@\" target=\"_blank\" rel=\"noopener noreferrer\">One<em class=\"\">Taq<\/em> DNA Polymerase<\/a> is an optimized blend of <a class=\"\" href=\"https:\/\/www.neb-online.fr\/en\/arts\/M0480@;M0267@;M0273@\" target=\"_blank\" rel=\"noopener noreferrer\"><em class=\"\">Taq<\/em><\/a> and <a class=\"\" href=\"https:\/\/www.neb-online.fr\/en\/art\/M0258@\" target=\"_blank\" rel=\"noopener noreferrer\">Deep Vent<\/a> DNA polymerases for use with routine and difficult PCR experiments. The 3\u00b4\u2192 5\u00b4 exonuclease activity of <a class=\"\" href=\"https:\/\/www.neb-online.fr\/en\/art\/M0258@\" target=\"_blank\" rel=\"noopener noreferrer\">Deep Vent DNA Polymerase<\/a> increases the fidelity and robust amplification of <a class=\"\" href=\"https:\/\/www.neb-online.fr\/en\/arts\/M0267;M0273@\" target=\"_blank\" rel=\"noopener noreferrer\"><em class=\"\">Taq<\/em> DNA Polymerase<\/a>. The One<em class=\"\">Taq <\/em>Reaction Buffers and High GC Enhancer have been formulated for robust yields with minimal optimization, regardless of a template\u2019s GC content.<\/p>\n<p class=\"\"><a class=\"\" href=\"https:\/\/www.neb-online.fr\/en\/art\/M0480@\" target=\"_blank\" rel=\"noopener noreferrer\">One<em class=\"\">Taq<\/em> DNA Polymerase<\/a> is supplied with two 5X buffers (Standard and GC), as well as a High GC Enhancer solution. For most routine and\/or AT-rich amplicons (Lambda, etc.) or complex amplicons with up to ~65% GC content, One<em class=\"\">Taq<\/em> Standard Reaction Buffer provides robust amplification. For GC-rich amplicons, the One<em class=\"\">Taq<\/em> GC Reaction Buffer can improve both performance and yield. For particularly high GC or difficult amplicons, the One<em class=\"\">Taq <\/em>High GC Enhancer can be added at a final concentration of 10\u201320% to reactions containing One<em class=\"\">Taq<\/em> GC Reaction Buffer.[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/3&#8243; css=&#8221;.vc_custom_1455611318959{border-top-width: 2px !important;border-bottom-width: 2px !important;padding-top: 20px !important;background-color: #f4f1e7 !important;border-top-color: #eb7e2d !important;border-top-style: solid !important;border-bottom-color: #eb7e2d !important;border-bottom-style: solid !important;}&#8221;][vc_single_image image=&#8221;5047&#8243; img_size=&#8221;full&#8221; alignment=&#8221;center&#8221;][vc_column_text]<\/p>\n<p class=\"\" style=\"text-align: center;\"><strong class=\"\">Do you want a free sample of One<em>Taq<\/em> Hot Start DNA Polymerase?<\/strong><\/p>\n<p>[\/vc_column_text]<a class=\"btn btn-standard form-btn\" target=\"_blank\" href=\"https:\/\/international.neb.com\/forms\/onetaq-sample-request\" style=\"margin-right:auto;\">Click here!<\/a>[\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;.vc_custom_1455792275840{border-top-width: 3px !important;border-right-width: 3px !important;border-bottom-width: 3px !important;border-left-width: 3px !important;padding-top: 20px !important;padding-right: 20px !important;padding-bottom: 20px !important;padding-left: 20px !important;border-left-color: #eb7e2d !important;border-left-style: solid !important;border-right-color: #eb7e2d !important;border-right-style: solid !important;border-top-color: #eb7e2d !important;border-top-style: solid !important;border-bottom-color: #eb7e2d !important;border-bottom-style: solid !important;}&#8221;]<span class=\"\" style=\"font-size: 1.3em;\"><strong class=\"\">New:<\/strong><\/span><br class=\"\" \/><span class=\"\" style=\"line-height: 200%; font-size: 1.3em;\"><strong class=\"\"><a class=\"\" href=\"https:\/\/www.neb-online.fr\/en\/art\/M0509@\" target=\"_blank\" rel=\"noopener noreferrer\">One<em class=\"\">Taq<\/em> Quick-Load DNA Polymerase<\/a> \u2013 After PCR directly onto the gel!<\/strong><\/span><br class=\"\" \/>Besides the conventional reaction buffer this new variant contains a colored Quick-Load reaction buffer for direct gel loading on agarose gels after PCR. One<em class=\"\">Taq<\/em> Quick-Load DNA Polymerase is therefore particularly well suited for routine PCR like genotyping, colony PCR etc. (up to 6 kb and ca. 55% GC content).<br class=\"\" \/>Quick \u2013 reliable \u2013 favorable!<\/p>\n<p class=\"\"><strong class=\"\">Whichever option you choose &#8211; the One<em class=\"\">Taq<\/em> DNA Polymerase is the unmatched standard for your everyday routine PCR.<br class=\"\" \/><\/strong><\/p>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_row_inner][vc_column_inner width=&#8221;1\/2&#8243; css=&#8221;.vc_custom_1455614174862{border-top-width: 2px !important;border-bottom-width: 2px !important;padding-top: 20px !important;padding-bottom: 20px !important;background-color: #f4f1e7 !important;border-top-color: #eb7e2d !important;border-top-style: solid !important;border-bottom-color: #eb7e2d !important;border-bottom-style: solid !important;}&#8221;][vc_column_text]<span class=\"\" style=\"font-size: 1.3em; color: #dd7500;\">Advantages:<\/span><\/p>\n<ul class=\"\">\n<li class=\"\">Ideal for routine, AT- and GC-rich PCR Applications<\/li>\n<\/ul>\n<p class=\"\"><span class=\"\" style=\"font-size: 1.3em; color: #dd7500;\">Applications:<\/span><\/p>\n<ul class=\"\">\n<li class=\"\">High Sensitivity PCR<\/li>\n<li class=\"\">High Throughput PCR<\/li>\n<li class=\"\">Routine PCR<\/li>\n<li class=\"\">GC-rich PCR<\/li>\n<li class=\"\">AT-rich PCR<\/li>\n<li class=\"\">Colony PCR<\/li>\n<li class=\"\">Long PCR (up to ~6 kb genomic)<\/li>\n<\/ul>\n<p class=\"\">[\/vc_column_text][\/vc_column_inner][vc_column_inner width=&#8221;1\/2&#8243;][vc_single_image image=&#8221;7490&#8243; img_size=&#8221;100&#215;130&#8243; alignment=&#8221;center&#8221; style=&#8221;vc_box_outline&#8221;][vc_column_text]<\/p>\n<p class=\"\" style=\"text-align: center;\"><strong class=\"\">Application Note:<\/strong><\/p>\n<p class=\"\" style=\"text-align: center;\"><strong class=\"\">&#8220;Robust Colony PCR from Multiple E. coli Strains using OneTaq<sup class=\"\">\u00ae<\/sup> Quick-Load<sup class=\"\">\u00ae<\/sup> Master Mixes&#8221;<\/strong><\/p>\n<p class=\"\">[\/vc_column_text]<a class=\"btn btn-standard form-btn\"  href=\"http:\/\/www.neb-online.fr\/wp-content\/uploads\/NEB_ColonyPCR_ApplicationNote.pdf\" style=\"margin-left:auto;margin-right:auto;\">Download PDF<\/a>[\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row css=&#8221;.vc_custom_1455613702583{background-color: #f4f1e7 !important;}&#8221;][vc_column][vc_separator color=&#8221;custom&#8221; accent_color=&#8221;#919191&#8243;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;2\/3&#8243; css=&#8221;.vc_custom_1429224315139{margin-bottom: 35px !important;}&#8221;][vc_column_text]<span class=\"bildunterschrift\"><em class=\"\">Amplification of a selection of sequences with varying GC content from human and C. elegansgenomic DNA using <a class=\"\" href=\"https:\/\/www.neb-online.fr\/en\/art\/M0480@\" target=\"_blank\" rel=\"noopener noreferrer\">OneTaq DNA Polymerase<\/a>. GC content is indicated above gel. Marker M is the 1<a class=\"\" href=\"https:\/\/www.neb-online.fr\/en\/art\/N3232@\" target=\"_blank\" rel=\"noopener noreferrer\"> kb DNA Ladder<\/a> .<\/em><\/span>[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/3&#8243;][vc_single_image image=&#8221;1258&#8243; img_size=&#8221;full&#8221; img_link_large=&#8221;yes&#8221; css=&#8221;.vc_custom_1455613577564{margin-bottom: 6px !important;}&#8221;][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator color=&#8221;custom&#8221; accent_color=&#8221;#919191&#8243;][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<\/p>\n<h2 class=\"\">One<em class=\"\">Taq<\/em> Hot-Start DNA Polymerase<\/h2>\n<p class=\"\"><a class=\"\" href=\"https:\/\/www.neb-online.fr\/en\/art\/M0481@\" target=\"_blank\" rel=\"noopener noreferrer\">One<em class=\"\">Taq<\/em>\u00ae Hot Start DNA Polymerase<\/a> is an optimized blend of <em class=\"\">Taq<\/em> and Deep VentR\u2122 DNA polymerases combined with an aptamer-based inhibitor.<br class=\"\" \/>The inhibitor binds reversibly, blocking polymerase activity at temperatures below 45\u00b0C. The polymerase is activated during normal cycling conditions, allowing reactions to be set up at room temperature. <a class=\"\" href=\"https:\/\/www.neb-online.fr\/en\/art\/M0481@\" target=\"_blank\" rel=\"noopener noreferrer\">One<em class=\"\">Taq<\/em> Hot Start DNA Polymerase<\/a> does not require a separate high temperature incubation step to activate the enzyme and can be used in typical <em class=\"\">Taq<\/em>-based cycling protocols.[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/6&#8243;][\/vc_column][vc_column width=&#8221;2\/3&#8243;][vc_column_text css=&#8221;.vc_custom_1432630150636{margin-bottom: 6px !important;}&#8221;]<b class=\"\">Prolonged incubation times at room temperature have no influence on the performance of OneTaq\u00ae Hot Start DNA Polymerase<\/b>[\/vc_column_text][vc_single_image image=&#8221;1263&#8243; img_size=&#8221;full&#8221; alignment=&#8221;center&#8221; css=&#8221;.vc_custom_1429221615612{margin-bottom: 6px !important;}&#8221;][vc_column_text]<span class=\"bildunterschrift\"><em class=\"\">Amplification of a selection of sequences with varying GC content from human and C. elegans genomic DNA using <a class=\"\" href=\"https:\/\/shop.neb-online.fr\/en\/art\/M0481@\" target=\"_blank\" rel=\"noopener noreferrer\">OneTaq Hot Start DNA Polymerase<\/a>. The presence or absence of an extended room temperature incubation does not affect performance. GC content is indicated below gel. Marker M is the <a class=\"\" href=\"https:\/\/www.neb-online.fr\/en\/art\/N3232@\" target=\"_blank\" rel=\"noopener noreferrer\">1 kb DNA Ladder<\/a> .<\/em><\/span>[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/6&#8243;][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;.vc_custom_1430137966778{margin-bottom: 6px !important;}&#8221;]<strong class=\"\">Comparison of recommended activation steps of commercially available Hot Start DNA Polymerases<\/strong>[\/vc_column_text][vc_column_text]<\/p>\n<table class=\"tableborder\" border=\"0\" cellspacing=\"0\" cellpadding=\"2\">\n<tbody class=\"\">\n<tr class=\"\" style=\"height: 25px;\">\n<td class=\"bodycopy tdborder\" style=\"font-weight: bold; color: #ffffff;\" align=\"left\" valign=\"middle\" bgcolor=\"#58585a\" width=\"187\">MANUFACTURER<\/td>\n<td class=\"bodycopy tdborder\" style=\"font-weight: bold; color: #ffffff;\" align=\"left\" valign=\"middle\" bgcolor=\"#58585a\" width=\"230\">ENZYME<\/td>\n<td class=\"bodycopy tdborder\" style=\"font-weight: bold; color: #ffffff;\" align=\"left\" valign=\"middle\" bgcolor=\"#58585a\" width=\"219\">ACTIVATION STEP*<\/td>\n<td class=\"bodycopy tdborder\" style=\"font-weight: bold; color: #ffffff;\" align=\"left\" valign=\"middle\" bgcolor=\"#58585a\" width=\"246\">HOT START FORM<\/td>\n<\/tr>\n<tr class=\"\" style=\"height: 25px;\">\n<td class=\"bodycopy tdborder\" valign=\"middle\">Applied BioSystems<\/td>\n<td class=\"bodycopy tdborder\" valign=\"middle\">Ampli<em class=\"\">Taq<\/em> Gold<sup class=\"\">\u00ae<\/sup> 360<\/td>\n<td class=\"bodycopy tdborder\" valign=\"middle\">10&#8242;, 95\u00b0C<\/td>\n<td class=\"bodycopy tdborder\" valign=\"middle\">Modified<\/td>\n<\/tr>\n<tr class=\"\" style=\"height: 25px;\">\n<td class=\"bodycopy tdborder\" valign=\"middle\" bgcolor=\"#dcddde\">Invitrogen<\/td>\n<td class=\"bodycopy tdborder\" valign=\"middle\" bgcolor=\"#dcddde\">Platinum<sup class=\"\">\u00ae<\/sup> <em class=\"\">Taq<\/em><\/td>\n<td class=\"bodycopy tdborder\" valign=\"middle\" bgcolor=\"#dcddde\">30&#8243;\u20132&#8242;, 94\u00b0C<\/td>\n<td class=\"bodycopy tdborder\" valign=\"middle\" bgcolor=\"#dcddde\">Ab<\/td>\n<\/tr>\n<tr class=\"\" style=\"height: 25px;\">\n<td class=\"bodycopy tdborder\" valign=\"middle\">Promega<\/td>\n<td class=\"bodycopy tdborder\" valign=\"middle\">Go<em class=\"\">Taq<\/em><sup class=\"\">\u00ae<\/sup> Hot Start<\/td>\n<td class=\"bodycopy tdborder\" valign=\"middle\">2&#8242;, 94\u201395\u00b0C<\/td>\n<td class=\"bodycopy tdborder\" valign=\"middle\">Ab<\/td>\n<\/tr>\n<tr class=\"\" style=\"height: 25px;\">\n<td class=\"bodycopy tdborder\" valign=\"middle\" bgcolor=\"#dcddde\">Qiagen<\/td>\n<td class=\"bodycopy tdborder\" valign=\"middle\" bgcolor=\"#dcddde\">HotStar<em class=\"\">Taq<\/em><\/td>\n<td class=\"bodycopy tdborder\" valign=\"middle\" bgcolor=\"#dcddde\">15&#8242;, 95\u00b0C<\/td>\n<td class=\"bodycopy tdborder\" valign=\"middle\" bgcolor=\"#dcddde\">Modified<\/td>\n<\/tr>\n<tr class=\"\" style=\"height: 25px;\">\n<td class=\"bodycopy tdborder\" valign=\"middle\">Roche<\/td>\n<td class=\"bodycopy tdborder\" valign=\"middle\">FastStart <em class=\"\">Taq<\/em><\/td>\n<td class=\"bodycopy tdborder\" valign=\"middle\">4&#8242;, 95\u00b0C<\/td>\n<td class=\"bodycopy tdborder\" valign=\"middle\">Modified<\/td>\n<\/tr>\n<tr class=\"\" style=\"height: 25px;\">\n<td class=\"bodycopy tdborder\" valign=\"middle\" bgcolor=\"#dcddde\">Sigma<\/td>\n<td class=\"bodycopy tdborder\" valign=\"middle\" bgcolor=\"#dcddde\">JumpStart\u2122 <em class=\"\">Taq<\/em><\/td>\n<td class=\"bodycopy tdborder\" valign=\"middle\" bgcolor=\"#dcddde\">1&#8242;, 94\u00b0C<\/td>\n<td class=\"bodycopy tdborder\" valign=\"middle\" bgcolor=\"#dcddde\">Ab<\/td>\n<\/tr>\n<tr class=\"\" style=\"height: 25px;\">\n<td class=\"bodycopy tdborder\" valign=\"middle\">Thermo Fisher<\/td>\n<td class=\"bodycopy tdborder\" valign=\"middle\">Thermo-Start <em class=\"\">Taq<\/em><\/td>\n<td class=\"bodycopy tdborder\" valign=\"middle\">15&#8242;, 95\u00b0C<\/td>\n<td class=\"bodycopy tdborder\" valign=\"middle\">Modified<\/td>\n<\/tr>\n<tr class=\"\" style=\"height: 25px;\">\n<td class=\"bodycopy tdborder\" valign=\"middle\" bgcolor=\"#f0e3d0\"><strong class=\"\"> NEB<\/strong><\/td>\n<td class=\"bodycopy tdborder\" valign=\"middle\" bgcolor=\"#f0e3d0\"><strong class=\"\"> One<em class=\"\">Taq<\/em><\/strong><\/td>\n<td class=\"bodycopy tdborder\" valign=\"middle\" bgcolor=\"#f0e3d0\"><strong class=\"\"> None<\/strong><\/td>\n<td class=\"bodycopy tdborder\" valign=\"middle\" bgcolor=\"#f0e3d0\"><strong class=\"\"> Aptamer<\/strong><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p class=\"\">[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<\/p>\n<h2 class=\"\">Product table<\/h2>\n<table style=\"width: 760px;\" border=\"0\" cellpadding=\"4\">\n<tbody class=\"\">\n<tr class=\"\" style=\"background-color: #f3b281;\">\n<th class=\"\" style=\"border-bottom: solid #eb7e2d;\" width=\"396\" height=\"40\">PRODUCT<\/th>\n<th class=\"\" style=\"border-bottom: solid #eb7e2d;\" width=\"80\">Prod.Nr.<\/th>\n<th class=\"\" style=\"border-bottom: solid #eb7e2d;\" width=\"132\">SIZE<\/th>\n<th class=\"\" style=\"border-bottom: solid #eb7e2d;\" colspan=\"1\" width=\"80\">PRICE<\/th>\n<\/tr>\n<tr class=\"\">\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"3\">One<em class=\"\">Taq<\/em><sup class=\"\">\u00ae<\/sup> DNA Polymerase<\/td>\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0480 S<\/td>\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">200 units<\/td>\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/m0480s\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter wp-image-4375 size-full\" src=\"https:\/\/www.neb-online.fr\/\/\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr class=\"\">\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0480 L<\/td>\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">1000 units<\/td>\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/m0480l\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter wp-image-4375 size-full\" src=\"http:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr class=\"\">\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0480 X<\/td>\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">5000 units<\/td>\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/m0480x\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter wp-image-4375 size-full\" src=\"http:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr class=\"\">\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"3\">One<em class=\"\">Taq<\/em><sup class=\"\">\u00ae<\/sup> Hot Start DNA Polymerase<\/td>\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0481 S<\/td>\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">200 units<\/td>\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/m0481s\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter wp-image-4375 size-full\" src=\"http:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr class=\"\">\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0481 L<\/td>\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">1000 units<\/td>\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/m0481l\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"wp-image-4375 size-full aligncenter\" src=\"http:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr class=\"\">\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0481 X<\/td>\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">5000 units<\/td>\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/m0481x\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter wp-image-4375 size-full\" src=\"http:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr class=\"\">\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"2\">One<em class=\"\">Taq<\/em><sup class=\"\">\u00ae<\/sup> 2X Master Mix with Standard Buffer<\/td>\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0482 S<\/td>\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 rxns<\/td>\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/m0482s\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter wp-image-4375 size-full\" src=\"http:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr class=\"\">\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0482 L<\/td>\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">500 rxns<\/td>\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/m0482l\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter wp-image-4375 size-full\" src=\"http:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr class=\"\">\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"2\">One<em class=\"\">Taq<\/em><sup class=\"\">\u00ae<\/sup> 2X Master Mix with GC Buffer<\/td>\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0483 S<\/td>\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 rxns<\/td>\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/m0483s\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter wp-image-4375 size-full\" src=\"http:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr class=\"\">\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0483 L<\/td>\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">500 rxns<\/td>\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/m0483l\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter wp-image-4375 size-full\" src=\"http:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr class=\"\">\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"2\">One<em class=\"\">Taq<\/em><sup class=\"\">\u00ae<\/sup> Hot Start 2X Master Mix with Standard Buffer<\/td>\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0484 S<\/td>\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 rxns<\/td>\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/m0484s\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter wp-image-4375 size-full\" src=\"http:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr class=\"\">\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0484 L<\/td>\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">500 rxns<\/td>\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/m0484l\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter wp-image-4375 size-full\" src=\"http:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr class=\"\">\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"2\">One<em class=\"\">Taq<\/em><sup class=\"\">\u00ae<\/sup> Hot Start 2X Master Mix with GC Buffer<\/td>\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0485 S<\/td>\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 rxns<\/td>\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/m0485s\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter wp-image-4375 size-full\" src=\"http:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr class=\"\">\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0485 L<\/td>\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">500 rxns<\/td>\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/m0485l\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter wp-image-4375 size-full\" src=\"http:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr class=\"\">\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"2\">One<em class=\"\">Taq<\/em><sup class=\"\">\u00ae<\/sup> Quick-Load 2X Master Mix with Standard Buffer<\/td>\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0486 S<\/td>\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 rxns<\/td>\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/m0486s\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter wp-image-4375 size-full\" src=\"http:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr class=\"\">\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0486 L<\/td>\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">500 rxns<\/td>\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/m0486l\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter wp-image-4375 size-full\" src=\"http:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr class=\"\">\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"2\">One<em class=\"\">Taq<\/em><sup class=\"\">\u00ae<\/sup> Hot Start Quick-Load 2X Master Mix, Standard Buffer<\/td>\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0488 S<\/td>\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 rxns<\/td>\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/m0488s\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter wp-image-4375 size-full\" src=\"http:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr class=\"\">\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0488 L<\/td>\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">500 rxns<\/td>\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/m0488l\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter wp-image-4375 size-full\" src=\"http:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr class=\"\">\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"2\">One<em class=\"\">Taq<\/em><sup class=\"\">\u00ae<\/sup> Hot Start Quick-Load 2X Master Mix with GC Buffer<\/td>\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0489 S<\/td>\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 rxns<\/td>\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/m0489s\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter wp-image-4375 size-full\" src=\"http:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr class=\"\">\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0489 L<\/td>\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">500 rxns<\/td>\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/m0489l\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter wp-image-4375 size-full\" src=\"http:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr class=\"\">\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"3\">One<em class=\"\">Taq<\/em><sup class=\"\">\u00ae<\/sup> Quick-Load<sup class=\"\">\u00ae<\/sup> DNA Polymerase<\/td>\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d;\">M0509 S<\/td>\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 units<\/td>\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/M0509s\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter wp-image-4375 size-full\" src=\"http:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr class=\"\">\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0509 L<\/td>\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">500 units<\/td>\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/M0509l\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter wp-image-4375 size-full\" src=\"http:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr class=\"\">\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0509 X<\/td>\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">2500 units<\/td>\n<td class=\"\" style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/M0509x\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter wp-image-4375 size-full\" src=\"http:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text][\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row][vc_column][vc_basic_grid post_type=&#8221;post&#8221; max_items=&#8221;10&#8243; element_width=&#8221;12&#8243; gap=&#8221;15&#8243; item=&#8221;4123&#8243; taxonomies=&#8221;74&#8243; grid_id=&#8221;vc_gid:1659014994409-8b82fd8a348ad64fa5743b514bcaea23-10&#8243;][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text][\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;2\/3&#8243; css=&#8221;.vc_custom_1429224286633{margin-bottom: 25px !important;}&#8221;][vc_column_text] OneTaq DNA Polymerase is an optimized blend of Taq and Deep Vent DNA polymerases for use with routine and difficult PCR experiments. The 3\u00b4\u2192 5\u00b4 exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase. The&#8230;  <a class=\"excerpt-read-more\" href=\"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/routine-pcr-en\/onetaq-dna-polymerase\/\" title=\"Read OneTaq DNA Polymerase\">Read more &raquo;<\/a><\/p>\n","protected":false},"author":15,"featured_media":1646,"parent":2107,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-sidebar-pcr-dna-amplification.php","meta":{"footnotes":""},"class_list":["post-2095","page","type-page","status-publish","has-post-thumbnail","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.0 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>OneTaq DNA Polymerase - New England Biolabs France<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/routine-pcr-en\/onetaq-dna-polymerase\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"OneTaq DNA Polymerase - New England Biolabs France\" \/>\n<meta property=\"og:description\" content=\"[vc_row][vc_column][vc_basic_grid post_type=&#8221;post&#8221; max_items=&#8221;10&#8243; element_width=&#8221;12&#8243; gap=&#8221;15&#8243; item=&#8221;4123&#8243; taxonomies=&#8221;74&#8243; grid_id=&#8221;vc_gid:1659014994409-8b82fd8a348ad64fa5743b514bcaea23-10&#8243;][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text][\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;2\/3&#8243; css=&#8221;.vc_custom_1429224286633{margin-bottom: 25px !important;}&#8221;][vc_column_text] OneTaq DNA Polymerase is an optimized blend of Taq and Deep Vent DNA polymerases for use with routine and difficult PCR experiments. The 3\u00b4\u2192 5\u00b4 exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase. The... Read more &raquo;\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/routine-pcr-en\/onetaq-dna-polymerase\/\" \/>\n<meta property=\"og:site_name\" content=\"New England Biolabs France\" \/>\n<meta property=\"article:modified_time\" content=\"2022-07-28T13:44:54+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/04\/NEB_PCR_onetaqx760px.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"760\" \/>\n\t<meta property=\"og:image:height\" content=\"200\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"10 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/routine-pcr-en\/onetaq-dna-polymerase\/\",\"url\":\"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/routine-pcr-en\/onetaq-dna-polymerase\/\",\"name\":\"OneTaq DNA Polymerase - New England Biolabs France\",\"isPartOf\":{\"@id\":\"https:\/\/www.neb-online.fr\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/routine-pcr-en\/onetaq-dna-polymerase\/#primaryimage\"},\"image\":{\"@id\":\"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/routine-pcr-en\/onetaq-dna-polymerase\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/04\/NEB_PCR_onetaqx760px.jpg\",\"datePublished\":\"2015-04-27T12:33:35+00:00\",\"dateModified\":\"2022-07-28T13:44:54+00:00\",\"breadcrumb\":{\"@id\":\"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/routine-pcr-en\/onetaq-dna-polymerase\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/routine-pcr-en\/onetaq-dna-polymerase\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/routine-pcr-en\/onetaq-dna-polymerase\/#primaryimage\",\"url\":\"https:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/04\/NEB_PCR_onetaqx760px.jpg\",\"contentUrl\":\"https:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/04\/NEB_PCR_onetaqx760px.jpg\",\"width\":760,\"height\":200,\"caption\":\"OneTaq Banner\"},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/routine-pcr-en\/onetaq-dna-polymerase\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"PCR and DNA Amplification\",\"item\":\"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Routine PCR\",\"item\":\"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/routine-pcr-en\/\"},{\"@type\":\"ListItem\",\"position\":3,\"name\":\"OneTaq DNA Polymerase\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/www.neb-online.fr\/#website\",\"url\":\"https:\/\/www.neb-online.fr\/\",\"name\":\"New England Biolabs France\",\"description\":\"be INSPIRED, drive DISCOVERY, stay GENUINE\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/www.neb-online.fr\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"OneTaq DNA Polymerase - New England Biolabs France","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/routine-pcr-en\/onetaq-dna-polymerase\/","og_locale":"en_US","og_type":"article","og_title":"OneTaq DNA Polymerase - New England Biolabs France","og_description":"[vc_row][vc_column][vc_basic_grid post_type=&#8221;post&#8221; max_items=&#8221;10&#8243; element_width=&#8221;12&#8243; gap=&#8221;15&#8243; item=&#8221;4123&#8243; taxonomies=&#8221;74&#8243; grid_id=&#8221;vc_gid:1659014994409-8b82fd8a348ad64fa5743b514bcaea23-10&#8243;][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text][\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;2\/3&#8243; css=&#8221;.vc_custom_1429224286633{margin-bottom: 25px !important;}&#8221;][vc_column_text] OneTaq DNA Polymerase is an optimized blend of Taq and Deep Vent DNA polymerases for use with routine and difficult PCR experiments. The 3\u00b4\u2192 5\u00b4 exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase. The... Read more &raquo;","og_url":"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/routine-pcr-en\/onetaq-dna-polymerase\/","og_site_name":"New England Biolabs France","article_modified_time":"2022-07-28T13:44:54+00:00","og_image":[{"width":760,"height":200,"url":"https:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/04\/NEB_PCR_onetaqx760px.jpg","type":"image\/jpeg"}],"twitter_misc":{"Est. reading time":"10 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/routine-pcr-en\/onetaq-dna-polymerase\/","url":"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/routine-pcr-en\/onetaq-dna-polymerase\/","name":"OneTaq DNA Polymerase - New England Biolabs France","isPartOf":{"@id":"https:\/\/www.neb-online.fr\/#website"},"primaryImageOfPage":{"@id":"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/routine-pcr-en\/onetaq-dna-polymerase\/#primaryimage"},"image":{"@id":"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/routine-pcr-en\/onetaq-dna-polymerase\/#primaryimage"},"thumbnailUrl":"https:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/04\/NEB_PCR_onetaqx760px.jpg","datePublished":"2015-04-27T12:33:35+00:00","dateModified":"2022-07-28T13:44:54+00:00","breadcrumb":{"@id":"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/routine-pcr-en\/onetaq-dna-polymerase\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/routine-pcr-en\/onetaq-dna-polymerase\/"]}]},{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/routine-pcr-en\/onetaq-dna-polymerase\/#primaryimage","url":"https:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/04\/NEB_PCR_onetaqx760px.jpg","contentUrl":"https:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/04\/NEB_PCR_onetaqx760px.jpg","width":760,"height":200,"caption":"OneTaq Banner"},{"@type":"BreadcrumbList","@id":"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/routine-pcr-en\/onetaq-dna-polymerase\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"PCR and DNA Amplification","item":"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/"},{"@type":"ListItem","position":2,"name":"Routine PCR","item":"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/routine-pcr-en\/"},{"@type":"ListItem","position":3,"name":"OneTaq DNA Polymerase"}]},{"@type":"WebSite","@id":"https:\/\/www.neb-online.fr\/#website","url":"https:\/\/www.neb-online.fr\/","name":"New England Biolabs France","description":"be INSPIRED, drive DISCOVERY, stay GENUINE","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/www.neb-online.fr\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"}]}},"_links":{"self":[{"href":"https:\/\/www.neb-online.fr\/en\/wp-json\/wp\/v2\/pages\/2095","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.neb-online.fr\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.neb-online.fr\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.neb-online.fr\/en\/wp-json\/wp\/v2\/users\/15"}],"replies":[{"embeddable":true,"href":"https:\/\/www.neb-online.fr\/en\/wp-json\/wp\/v2\/comments?post=2095"}],"version-history":[{"count":5,"href":"https:\/\/www.neb-online.fr\/en\/wp-json\/wp\/v2\/pages\/2095\/revisions"}],"predecessor-version":[{"id":23984,"href":"https:\/\/www.neb-online.fr\/en\/wp-json\/wp\/v2\/pages\/2095\/revisions\/23984"}],"up":[{"embeddable":true,"href":"https:\/\/www.neb-online.fr\/en\/wp-json\/wp\/v2\/pages\/2107"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.neb-online.fr\/en\/wp-json\/wp\/v2\/media\/1646"}],"wp:attachment":[{"href":"https:\/\/www.neb-online.fr\/en\/wp-json\/wp\/v2\/media?parent=2095"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}