{"id":2100,"date":"2015-04-27T12:47:16","date_gmt":"2015-04-27T12:47:16","guid":{"rendered":"http:\/\/www.neb-online.fr\/?page_id=2100"},"modified":"2022-11-07T11:52:53","modified_gmt":"2022-11-07T10:52:53","slug":"taq-dna-polymerases","status":"publish","type":"page","link":"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/routine-pcr-en\/taq-dna-polymerases\/","title":{"rendered":"Taq DNA Polymerases"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]<\/p>\n<h2><em>Taq<\/em> DNA Polymerase<\/h2>\n<p><a href=\"https:\/\/shop.neb-online.fr\/en\/arts\/M0267@;M0273@;M0321@;E5000@;M0320@;M0270@;M0285@\" target=\"_blank\" rel=\"noopener noreferrer\"><em>Taq<\/em>\u00a0DNA Polymerase <\/a>is commonly used for routine PCR applications in many molecular biology labs.<\/p>\n<p>For your &#8220;daily&#8221; PCR we recommend NEBs reliable <a href=\"https:\/\/www.neb-online.fr\/en\/arts\/M0267@;M0273@;M0321@;E5000@;M0320@;M0270@;M0285@\" target=\"_blank\" rel=\"noopener noreferrer\">Taq DNA Polymerase<\/a>, which is available in various Buffer- and Enzyme-formulations.[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/2&#8243;]<div class=\"vbox\">\n\t\t\t\t<span class=\"vhead\">Advantages:<\/span>\n\t\t\t\t\t<ul class=\"vlist\">\n\t\t\t\t\t\t<li><span>Isolated from a recombinant source<br \/><\/span><\/li><li><span>Supplied with 10X Reaction Buffer<br \/><\/span><\/li><li><span>Robust and reliable reactions<br \/><\/span><\/li><li><span>Tolerates a wide range of templates<br \/><\/span><\/li><li><span>Incorporates dUTP, dITP and fluorescently-labeled nucleotides<br \/><\/span><\/li><li><span>Exceptional value in terms of cost per unit<\/span><\/li>\n\t\t\t\t\t<\/ul>\n\t\t\t<\/div>[\/vc_column][\/vc_row][vc_row][vc_column][vc_separator color=&#8221;black&#8221;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/3&#8243;][vc_column_text]<\/p>\n<h2>Hot Start <em>Taq<\/em> DNA Polymerase<\/h2>\n<p><a href=\"https:\/\/www.neb-online.fr\/en\/arts\/M0495@;M0496@\" target=\"_blank\" rel=\"noopener noreferrer\">Hot Start\u00a0<em>Taq\u00a0<\/em>DNA Polymerase<\/a> is a mixture of\u00a0<em>Taq<\/em>\u00a0DNA Polymerase and an aptamer-based inhibitor. The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 45\u00b0C, but releases the enzyme during normal cycling conditions, allowing reactions to be set up at room temperature. This aptamer-based hot start does not require a separate high temperature incubation step to activate the enzyme.[\/vc_column_text][\/vc_column][vc_column width=&#8221;2\/3&#8243;][vc_column_text]<strong>NEB&#8217;s Hot Start Taq shows excellent yields while being highly specific<\/strong>[\/vc_column_text][vc_column_text]<\/p>\n<table style=\"width: 760px; border: 1px solid #e6e6e6;\" border=\"1\" cellspacing=\"0\" cellpadding=\"2\">\n<tbody>\n<tr>\n<td rowspan=\"2\"><span style=\"font-size: 10pt;\"><b>Manufacturer<\/b><\/span><\/td>\n<td rowspan=\"2\"><strong><span style=\"font-size: 10pt;\">DNA Polymerase<\/span><\/strong><\/td>\n<td rowspan=\"2\"><strong><span style=\"font-size: 10pt;\">Hot Start Inhibitor<\/span><\/strong><\/td>\n<td style=\"text-align: center;\" rowspan=\"2\"><strong><span style=\"font-size: 10pt;\">Activation time**<\/span><\/strong><\/td>\n<td style=\"text-align: center;\" colspan=\"2\"><strong><span style=\"font-size: 10pt;\">Performance*<\/span><\/strong><\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\"><strong><span style=\"font-size: 10pt;\">Specificity<\/span><\/strong><\/td>\n<td style=\"text-align: center;\"><strong><span style=\"font-size: 10pt;\">Yield<\/span><\/strong><\/td>\n<\/tr>\n<tr>\n<td><span style=\"font-size: 10pt;\">Applied Biosystens<\/span><\/td>\n<td><span style=\"font-size: 10pt;\">AmpliTaq Gold 360<\/span><\/td>\n<td><span style=\"font-size: 10pt;\">Chem<\/span><\/td>\n<td style=\"text-align: center;\"><span style=\"font-size: 10pt;\">10 min<\/span><\/td>\n<td style=\"text-align: center;\"><img decoding=\"async\" src=\"\/wp-content\/uploads\/2015\/04\/NEB_DNA_HSTaq_3.jpg\" alt=\"NEB HSTaq_3\" width=\"10\" height=\"10\" \/><\/td>\n<td style=\"text-align: center;\"><img decoding=\"async\" style=\"border: 0px initial initial;\" src=\"\/wp-content\/uploads\/2015\/04\/NEB_DNA_HSTaq_3.jpg\" alt=\"NEB HSTaq_3\" width=\"10\" height=\"10\" \/><\/td>\n<\/tr>\n<tr>\n<td><span style=\"font-size: 10pt;\">Invitrogen<\/span><\/td>\n<td><span style=\"font-size: 10pt;\">Platinum\u00a0<em>Taq\u00a0<\/em><\/span><\/td>\n<td><span style=\"font-size: 10pt;\">Ab<\/span><\/td>\n<td style=\"text-align: center;\"><span style=\"font-size: 10pt;\">30 sec<\/span><\/td>\n<td style=\"text-align: center;\"><img decoding=\"async\" src=\"\/wp-content\/uploads\/2015\/04\/NEB_DNA_HSTaq_1.jpg\" alt=\"NEB HSTaq_1\" width=\"10\" height=\"10\" \/><\/td>\n<td style=\"text-align: center;\"><img loading=\"lazy\" decoding=\"async\" src=\"\/wp-content\/uploads\/2015\/04\/NEB_DNA_HSTaq_2.jpg\" alt=\"NEB HSTaq_2\" width=\"10\" height=\"10\" \/><\/td>\n<\/tr>\n<tr>\n<td><span style=\"color: #7f3f1f;\"><strong><span style=\"font-size: 10pt;\">NEB<\/span><\/strong><\/span><\/td>\n<td><span style=\"color: #7f3f1f;\"><strong><span style=\"font-size: 10pt;\">Hot Start\u00a0<em>Taq<\/em><\/span><\/strong><\/span><\/td>\n<td><span style=\"color: #7f3f1f;\"><strong><span style=\"font-size: 10pt;\">Aptamer<\/span><\/strong><\/span><\/td>\n<td style=\"text-align: center;\"><span style=\"color: #7f3f1f;\"><strong><span style=\"font-size: 10pt;\">None<\/span><\/strong><\/span><\/td>\n<td style=\"text-align: center;\"><img loading=\"lazy\" decoding=\"async\" src=\"\/wp-content\/uploads\/2015\/04\/NEB_DNA_HSTaq_4.jpg\" alt=\"NEB HSTaq_4\" width=\"10\" height=\"10\" \/><\/td>\n<td style=\"text-align: center;\"><img decoding=\"async\" style=\"border: 0px initial initial;\" src=\"\/wp-content\/uploads\/2015\/04\/NEB_DNA_HSTaq_3.jpg\" alt=\"NEB HSTaq_3\" width=\"10\" height=\"10\" \/><\/td>\n<\/tr>\n<tr>\n<td><span style=\"font-size: 10pt;\">Promega<\/span><\/td>\n<td><span style=\"font-size: 10pt;\">GoTaq Hot<\/span><\/td>\n<td><span style=\"font-size: 10pt;\">Ab<\/span><\/td>\n<td style=\"text-align: center;\"><span style=\"font-size: 10pt;\">2 min<\/span><\/td>\n<td style=\"text-align: center;\"><img loading=\"lazy\" decoding=\"async\" style=\"border: 0px initial initial;\" src=\"\/wp-content\/uploads\/2015\/04\/NEB_DNA_HSTaq_1.jpg\" alt=\"NEB HSTaq_1\" width=\"10\" height=\"10\" \/><\/td>\n<td style=\"text-align: center;\"><img loading=\"lazy\" decoding=\"async\" style=\"border: 0px initial initial;\" src=\"\/wp-content\/uploads\/2015\/04\/NEB_DNA_HSTaq_1.jpg\" alt=\"NEB HSTaq_1\" width=\"10\" height=\"10\" \/><\/td>\n<\/tr>\n<tr>\n<td><span style=\"font-size: 10pt;\">Qiagen<\/span><\/td>\n<td><span style=\"font-size: 10pt;\">Hot Star\u00a0<em>Taq<\/em><\/span><\/td>\n<td><span style=\"font-size: 10pt;\">Chem<\/span><\/td>\n<td style=\"text-align: center;\"><span style=\"font-size: 10pt;\">15 min<\/span><\/td>\n<td style=\"text-align: center;\"><img loading=\"lazy\" decoding=\"async\" style=\"border: 0px initial initial;\" src=\"\/wp-content\/uploads\/2015\/04\/NEB_DNA_HSTaq_1.jpg\" alt=\"NEB HSTaq_1\" width=\"10\" height=\"10\" \/><\/td>\n<td style=\"text-align: center;\"><img decoding=\"async\" style=\"border: 0px initial initial;\" src=\"\/wp-content\/uploads\/2015\/04\/NEB_DNA_HSTaq_3.jpg\" alt=\"NEB HSTaq_3\" width=\"10\" height=\"10\" \/><\/td>\n<\/tr>\n<tr>\n<td><span style=\"font-size: 10pt;\">Roche<\/span><\/td>\n<td><span style=\"font-size: 10pt;\">FastStart\u00a0<em>Taq<\/em><\/span><\/td>\n<td><span style=\"font-size: 10pt;\">Chem<\/span><\/td>\n<td style=\"text-align: center;\"><span style=\"font-size: 10pt;\">4 min<\/span><\/td>\n<td style=\"text-align: center;\"><img decoding=\"async\" style=\"border: 0px initial initial;\" src=\"\/wp-content\/uploads\/2015\/04\/NEB_DNA_HSTaq_3.jpg\" alt=\"NEB HSTaq_3\" width=\"10\" height=\"10\" \/><\/td>\n<td style=\"text-align: center;\"><img loading=\"lazy\" decoding=\"async\" style=\"border: 0px initial initial;\" src=\"\/wp-content\/uploads\/2015\/04\/NEB_DNA_HSTaq_4.jpg\" alt=\"NEB HSTaq_4\" width=\"10\" height=\"10\" \/><\/td>\n<\/tr>\n<tr>\n<td><span style=\"font-size: 10pt;\">Sigma<\/span><\/td>\n<td><span style=\"font-size: 10pt;\">JumpStart\u00a0<em>Taq<\/em><\/span><\/td>\n<td><span style=\"font-size: 10pt;\">Ab<\/span><\/td>\n<td style=\"text-align: center;\"><span style=\"font-size: 10pt;\">1 min<\/span><\/td>\n<td style=\"text-align: center;\"><img decoding=\"async\" style=\"border: 0px initial initial;\" src=\"\/wp-content\/uploads\/2015\/04\/NEB_DNA_HSTaq_3.jpg\" alt=\"NEB HSTaq_3\" width=\"10\" height=\"10\" \/><\/td>\n<td style=\"text-align: center;\"><img loading=\"lazy\" decoding=\"async\" style=\"border: 0px initial initial;\" src=\"\/wp-content\/uploads\/2015\/04\/NEB_DNA_HSTaq_4.jpg\" alt=\"NEB HSTaq_4\" width=\"10\" height=\"10\" \/><\/td>\n<\/tr>\n<tr>\n<td><span style=\"font-size: 10pt;\">Thermo Scientific<\/span><\/td>\n<td><span style=\"font-size: 10pt;\">Maxima Hot Start\u00a0<em>Taq<\/em><\/span><\/td>\n<td><span style=\"font-size: 10pt;\">Chem<\/span><\/td>\n<td style=\"text-align: center;\"><span style=\"font-size: 10pt;\">4 min<\/span><\/td>\n<td style=\"text-align: center;\"><img loading=\"lazy\" decoding=\"async\" style=\"border: 0px initial initial;\" src=\"\/wp-content\/uploads\/2015\/04\/NEB_DNA_HSTaq_2.jpg\" alt=\"NEB HSTaq_2\" width=\"10\" height=\"10\" \/><\/td>\n<td style=\"text-align: center;\"><img loading=\"lazy\" decoding=\"async\" style=\"border: 0px initial initial;\" src=\"\/wp-content\/uploads\/2015\/04\/NEB_DNA_HSTaq_2.jpg\" alt=\"NEB HSTaq_2\" width=\"10\" height=\"10\" \/><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/3&#8243;][\/vc_column][vc_column width=&#8221;1\/3&#8243;][vc_single_image image=&#8221;1590&#8243; img_size=&#8221;full&#8221;][\/vc_column][vc_column width=&#8221;1\/3&#8243;][vc_column_text]<span style=\"font-size: 8pt;\">*Performance compared to all enzymes tested. Results are from triplicate reactions on a series of human genomic amplicons.\u00a0Performance was scored for both yield and desired product. <\/span><span style=\"font-size: 8pt;\">**Time required to activate hot start enzymes may include initiation denaturation time, if not specified.<\/span>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator color=&#8221;black&#8221;][\/vc_column][\/vc_row][vc_row][vc_column css=&#8221;.vc_custom_1438326271449{background-color: #f4f1e7 !important;}&#8221;][vc_row_inner][vc_column_inner width=&#8221;2\/3&#8243; css=&#8221;.vc_custom_1438326279056{padding-top: 30px !important;}&#8221;][vc_column_text]<\/p>\n<p style=\"text-align: center;\"><strong>Do you want a free sample?<\/strong><\/p>\n<p>[\/vc_column_text]<a class=\"btn btn-standard form-btn\"  href=\"https:\/\/international.neb.com\/forms\/onetaq-sample-request\" style=\"margin-left:auto;margin-right:auto;\">Click here!<\/a>[\/vc_column_inner][vc_column_inner width=&#8221;1\/3&#8243;][vc_single_image image=&#8221;5047&#8243; img_size=&#8221;full&#8221; alignment=&#8221;center&#8221;][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text] Taq DNA Polymerase Taq\u00a0DNA Polymerase is commonly used for routine PCR applications in many molecular biology labs. For your &#8220;daily&#8221; PCR we recommend NEBs reliable Taq DNA Polymerase, which is available in various Buffer- and Enzyme-formulations.[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/2&#8243;][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator color=&#8221;black&#8221;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/3&#8243;][vc_column_text] Hot Start Taq DNA Polymerase Hot Start\u00a0Taq\u00a0DNA Polymerase is a mixture of\u00a0Taq\u00a0DNA Polymerase and&#8230;  <a class=\"excerpt-read-more\" href=\"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/routine-pcr-en\/taq-dna-polymerases\/\" title=\"Read Taq DNA Polymerases\">Read more &raquo;<\/a><\/p>\n","protected":false},"author":15,"featured_media":1406,"parent":2107,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-sidebar-pcr-dna-amplification.php","meta":{"footnotes":""},"class_list":["post-2100","page","type-page","status-publish","has-post-thumbnail","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.0 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Taq DNA Polymerases - New England Biolabs France<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/routine-pcr-en\/taq-dna-polymerases\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Taq DNA Polymerases - New England Biolabs France\" \/>\n<meta property=\"og:description\" content=\"[vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text] Taq DNA Polymerase Taq\u00a0DNA Polymerase is commonly used for routine PCR applications in many molecular biology labs. For your &#8220;daily&#8221; PCR we recommend NEBs reliable Taq DNA Polymerase, which is available in various Buffer- and Enzyme-formulations.[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/2&#8243;][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator color=&#8221;black&#8221;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/3&#8243;][vc_column_text] Hot Start Taq DNA Polymerase Hot Start\u00a0Taq\u00a0DNA Polymerase is a mixture of\u00a0Taq\u00a0DNA Polymerase and... 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For your &#8220;daily&#8221; PCR we recommend NEBs reliable Taq DNA Polymerase, which is available in various Buffer- and Enzyme-formulations.[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/2&#8243;][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator color=&#8221;black&#8221;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/3&#8243;][vc_column_text] Hot Start Taq DNA Polymerase Hot Start\u00a0Taq\u00a0DNA Polymerase is a mixture of\u00a0Taq\u00a0DNA Polymerase and... 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