{"id":2109,"date":"2015-04-27T13:08:55","date_gmt":"2015-04-27T13:08:55","guid":{"rendered":"http:\/\/www.neb-online.fr\/?page_id=2109"},"modified":"2021-03-26T17:56:33","modified_gmt":"2021-03-26T16:56:33","slug":"q5-high-fidelity-dna-polymerase","status":"publish","type":"page","link":"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/high-fidelity-pcr\/q5-high-fidelity-dna-polymerase\/","title":{"rendered":"Q5 High Fidelity DNA Polymerase"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row][vc_column][vc_column_text][\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<\/p>\n<h2>Fidelity at its finest<\/h2>\n<p>[\/vc_column_text][\/vc_column][vc_column width=&#8221;2\/3&#8243; css=&#8221;.vc_custom_1429281526171{margin-bottom: 12px !important;}&#8221;][vc_column_text]<a href=\"https:\/\/shop.neb-online.fr\/en\/art\/M0491@\" target=\"_blank\" rel=\"noopener noreferrer\">Q5\u00ae High-Fidelity DNA Polymerase<\/a> sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available\u00a0(&gt;100 times higher than\u00a0<a title=\"Taq DNA Polymerases\" href=\"http:\/\/www.neb-online.fr\/neb\/pcr-and-dna-amplification\/routine-pcr-en\/taq-dna-polymerases\/\"><em>Taq<\/em><\/a>), Q5 DNA Polymerase results in ultra-low\u00a0error rates.\u00a0Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.<\/p>\n<p><strong>Advantages<\/strong><\/p>\n<ul>\n<li>Highest fidelity amplification (~280x higher than\u00a0<em>Taq<\/em>)<\/li>\n<li>Ultra-low error rates<\/li>\n<li>Superior performance for a broad range of amplicons (from high AT to high GC)<\/li>\n<li><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/M0493@\" target=\"_blank\" rel=\"noopener noreferrer\">Hot start<\/a> and master mix formats available<\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/3&#8243; offset=&#8221;vc_hidden-xs&#8221;][vc_single_image image=&#8221;1322&#8243; img_size=&#8221;full&#8221; onclick=&#8221;custom_link&#8221; img_link_target=&#8221;_blank&#8221; link=&#8221;\/wp-content\/uploads\/2015\/04\/NEB_Polymerase_Fidelity_Article_2012.pdf&#8221;][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text][\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/2&#8243; css=&#8221;.vc_custom_1429281526171{margin-bottom: 12px !important;}&#8221;][vc_column_text]The Q5 buffer system is designed to provide superior performance with minimal optimization across a broad range of amplicons, regardless of GC content. For routine or complex amplicons up to ~65% GC content, Q5 Reaction Buffer\u00a0provides reliable and robust amplification. For amplicons with high GC content (&gt;65% GC), addition of the Q5 High GC Enhancer ensures continued maximum performance. Q5 and Q5 Hot Start DNA Polymerases are available as standalone enzymes, or in a master mix format for added convenience. Master mix formulations include dNTPs, Mg<sup>++<\/sup>\u00a0and all necessary buffer components.[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/2&#8243;][vc_column_text css=&#8221;.vc_custom_1502115612035{margin-bottom: 4px !important;}&#8221;]<\/p>\n<h4>Comparison of High-Fidelity Polymerases<strong>:<\/strong> <img fetchpriority=\"high\" decoding=\"async\" class=\"alignnone size-medium wp-image-9435\" src=\"http:\/\/www.neb-online.fr\/wp-content\/uploads\/Q5_Fidelity_Graph_0517-300x285.gif\" alt=\"\" width=\"300\" height=\"285\" \/><\/h4>\n<p>[\/vc_column_text][vc_column_text]<span class=\"bildunterschrift\"><em><sup>1<\/sup> We continue to investigate improved assays to characterize Q5\u2019s very low error rate to ensure that<br \/>\nwe present the most accurate fidelity data possible (Potapov, V. and Ong, J.L. (2017) PLoS<br \/>\nONE. 12(1): e0169774.).<\/em><br \/>\n<em><sup>2<\/sup> PCR-based mutation screening in lacZ (NEB), lacI (Agilent) or rpsL (Life)<\/em><br \/>\n<em><sup>3<\/sup> Takagi et al (1997) Appl. Env. Microbiol. 63, 4504-4510.<\/em><\/span>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][\/vc_column][\/vc_row][vc_row][vc_column css=&#8221;.vc_custom_1429281526171{margin-bottom: 12px !important;}&#8221;][vc_column_text]<\/p>\n<h2>Q5 Hot Start High-Fidelity DNA Polymerase<\/h2>\n<p>In contrast to chemically modified or antibody-based hot start polymerases, NEB&#8217;s <a href=\"https:\/\/shop.neb-online.fr\/en\/art\/M0493@\" target=\"_blank\" rel=\"noopener noreferrer\">Q5 Hot Start<\/a>\u00a0utilizes a unique synthetic aptamer. This molecule binds to the polymerase through non-covalent interactions, blocking activity during the reaction setup. The polymerase is activated during normal cycling conditions, allowing reactions to be set up at room temperature. Q5 Hot Start does not require a separate high temperature activation step, shortening reaction times and increasing ease-of-use. Q5 Hot Start Polymerase is an ideal choice for high specificity amplification and provides robust amplification of a wide variety of amplicons, regardless of GC content.[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator color=&#8221;custom&#8221; accent_color=&#8221;#919191&#8243;][\/vc_column][\/vc_row][vc_row][vc_column css=&#8221;.vc_custom_1439903887292{background-color: #f4f1e7 !important;}&#8221;][vc_row_inner][vc_column_inner width=&#8221;2\/3&#8243;][vc_column_text css=&#8221;.vc_custom_1439904640043{margin-top: 40px !important;margin-left: 30px !important;}&#8221;]<\/p>\n<h5>Free sample<\/h5>\n<p>You want a free sample? Contact us and request your personal free sample.[\/vc_column_text]<a class=\"btn btn-standard form-btn\"  href=\"https:\/\/www.neb.com\/unique-url\/q5-sample-request\" style=\"margin-right:auto;\">Click here<\/a>[\/vc_column_inner][vc_column_inner width=&#8221;1\/3&#8243;][vc_single_image image=&#8221;5047&#8243; img_size=&#8221;full&#8221; alignment=&#8221;center&#8221; css=&#8221;.vc_custom_1439904664806{margin-top: 20px !important;}&#8221;][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator color=&#8221;custom&#8221; accent_color=&#8221;#919191&#8243;][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<\/p>\n<h2>Details<\/h2>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;.vc_custom_1432307281545{margin-bottom: 5px !important;}&#8221;]<b>Figure 1: \u00a0<\/b><b>Robust amplification with <a href=\"https:\/\/shop.neb-online.fr\/en\/art\/M0491@\" target=\"_blank\" rel=\"noopener noreferrer\">Q5<\/a> (A) and <a href=\"https:\/\/shop.neb-online.fr\/en\/art\/M0493@\" target=\"_blank\" rel=\"noopener noreferrer\">Q5 Hot Start<\/a> (B) High-Fidelity DNA Polymerases, regardless of GC content<\/b>[\/vc_column_text][vc_single_image image=&#8221;1324&#8243; img_size=&#8221;full&#8221; css=&#8221;.vc_custom_1429280446845{margin-bottom: 5px !important;}&#8221;][vc_column_text]<span class=\"bildunterschrift\"><em>Amplification of a variety of human genomic amplicons from low to high GC content using either Q5 or Q5 Hot Start High-Fidelity DNA Polymerase. Reactions using Q5 Hot Start were set up at room temperature. All reactions were conducted using 30 cycles of amplification and visualized by microfluidic LabChip\u00ae analysis.<\/em><\/span>[\/vc_column_text][vc_column_text css=&#8221;.vc_custom_1429280524252{margin-bottom: 5px !important;}&#8221;]<strong>Figure 2: \u00a0<\/strong><strong>Amplification Performance Across a Wide Range of Genomic Targets<\/strong>[\/vc_column_text][vc_single_image image=&#8221;1327&#8243; img_size=&#8221;full&#8221; css=&#8221;.vc_custom_1429280529483{margin-bottom: 5px !important;}&#8221;][vc_column_text]<span class=\"bildunterschrift\"><em>PCR was performed with a variety of amplicons, with GC content ranging from high AT to high GC, with Q5 and several other commercially available polymerases. All polymerases were cycled according to manufacturer&#8217;s recommendations, including use of GC Buffers and enhancers when recommended. Yield and purity of reaction products were quantitated and represented, as shown in the figure key, by dot color and size. A large dark green dot represents the most successful performance. Q5 provides superior performance across the range of GC content.<\/em><\/span>[\/vc_column_text][vc_column_text css=&#8221;.vc_custom_1429280893150{margin-bottom: 5px !important;}&#8221;]<strong>Figure 3: Q5 DNA Polymerase offers superior amplification for a wide range of templates, regardless of GC content<\/strong>[\/vc_column_text][vc_single_image image=&#8221;1325&#8243; img_size=&#8221;full&#8221; css=&#8221;.vc_custom_1429280918110{margin-bottom: 5px !important;}&#8221;][vc_column_text]<span class=\"bildunterschrift\"><em>Amplification of a variety of human genomic amplicons from low to high GC content demonstrates the broad performance of <a href=\"https:\/\/shop.neb-online.fr\/en\/art\/M0491@\" target=\"_blank\" rel=\"noopener noreferrer\">Q5 High-Fidelity DNA Polymerase<\/a>. All reactions were conducted using 20 ng of input template and included 30 cycles of amplification. Results were visualized by microfluidic LabChip\u00ae analysis. Competitor polymerases were cycled according to manufacturer\u2019s recommendations. For the final three amplicons, GC Buffers or enhancers were used when supplied with the polymerase.<\/em><\/span>[\/vc_column_text][vc_column_text css=&#8221;.vc_custom_1429280979973{margin-bottom: 5px !important;}&#8221;]<strong>Figure 4:\u00a0Q5 Hot Start DNA Polymerase offers superior amplification for a wide range of templates, regardless of GC content Amplification<\/strong>[\/vc_column_text][vc_single_image image=&#8221;1326&#8243; img_size=&#8221;full&#8221; css=&#8221;.vc_custom_1429280973738{margin-bottom: 5px !important;}&#8221;][vc_column_text]<span class=\"bildunterschrift\"><em>Amplification of a variety of human genomic amplicons from low to high GC content demonstrates the broad performance of <a href=\"https:\/\/shop.neb-online.fr\/en\/art\/M0493@\" target=\"_blank\" rel=\"noopener noreferrer\">Q5 Hot Start High-Fidelity DNA Polymerase<\/a>. All reactions were set up at room temperature using 20 ng of input template and included 30 amplification cycles. Results were visualized by microfluidic LabChip\u00ae analysis. Competitor polymerases were cycled according to manufacturer\u2019s recommendations. For the final three amplicons, GC Buffers or enhancers were used when provided with the polymerase.<\/em><\/span>[\/vc_column_text][vc_column_text css=&#8221;.vc_custom_1502117368793{margin-bottom: 5px !important;}&#8221;]<strong>Table: Comparison of\u00a0HighFidelity DNA Polymerases<\/strong>[\/vc_column_text][vc_single_image image=&#8221;1329&#8243; img_size=&#8221;full&#8221; css=&#8221;.vc_custom_1429280874218{margin-bottom: 5px !important;}&#8221;][vc_column_text]<span class=\"bildunterschrift\"><em><sup>1<\/sup> PCR-based mutation screening in lacZ (NEB), lacI (Agilent) or rpsL (Life)<br \/>\n<\/em><em><sup>2<\/sup> Takagi et al (1997) Appl. Env. Microbiol. 63, 4504-4510.<br \/>\n<\/em><em><sup>3<\/sup> Template not specified.<br \/>\n<\/em><em><sup>4<\/sup> Simple templates include plasmid, viral and E. coli genomic DNA. Complex templates include plant, human and other mammalian genomic DNA.<br \/>\n<\/em><em><sup>5<\/sup> Values provided by individual manufacturers.<\/em><\/span>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator color=&#8221;custom&#8221; accent_color=&#8221;#919191&#8243;][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<\/p>\n<h2>Available Products<\/h2>\n<p>[\/vc_column_text][vc_column_text]<\/p>\n<table style=\"width: 760px;\" border=\"0\" cellpadding=\"5\">\n<tbody>\n<tr style=\"background-color: #f3b281;\">\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"328\" height=\"40px\">PRODUCT<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"82\">REF.<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"131\">SIZE<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" colspan=\"1\">PRICE<\/th>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Q5 High Fidelity DNA Polymerase<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0491S\/L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100\/500 units<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"28\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/m0491@\"><img decoding=\"async\" class=\"size-full wp-image-4375 aligncenter\" src=\"http:\/\/www.neb-online.fr\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Q5 High Fidelity 2X Master Mix<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0492S\/L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100\/500 units<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/m0492@\"><img decoding=\"async\" class=\"size-full wp-image-4375 aligncenter\" src=\"http:\/\/www.neb-online.fr\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Q5 Hot Start High Fidelity DNA Polymerase<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0493S\/L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100\/500 units<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/m0493@\"><img decoding=\"async\" class=\"size-full wp-image-4375 aligncenter\" src=\"http:\/\/www.neb-online.fr\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Q5 Hot Start High Fidelity 2X Master Mix<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0494S\/L\/X<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100\/500\/500 rxn<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/m0494@\"><img decoding=\"async\" class=\"size-full wp-image-4375 aligncenter\" src=\"http:\/\/www.neb-online.fr\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; padding-top: 30px;\" colspan=\"5\"><strong>Also available as Q5 High-Fidelity PCR Kit:<\/strong><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Q5 High-Fidelity PCR Kit<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E0555S\/L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">50\/200 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/e0555@\"><img decoding=\"async\" class=\"size-full wp-image-4375 aligncenter\" src=\"http:\/\/www.neb-online.fr\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; padding-top: 30px;\" colspan=\"5\"><strong>Also avalable as Next-Gen-Seq optimized formula:<\/strong><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">NEBNext High Fidelity 2X PCR Master Mix<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0541S\/L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">50\/250 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/m0541@\"><img decoding=\"async\" class=\"size-full wp-image-4375 aligncenter\" src=\"http:\/\/www.neb-online.fr\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><strong>Also available:<\/strong><\/p>\n<table style=\"width: 760px;\" border=\"0\" cellpadding=\"5\">\n<tbody>\n<tr style=\"background-color: #f3b281;\">\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"396\" height=\"40px\">PRODUCT<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"80\">REF.<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"132\">SIZE<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" colspan=\"1\" width=\"152\">PRICE<\/th>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Deoxynucleotide Solutions, Mix<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">N0447S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">8 \u00b5mol each<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/N0447s\"><img decoding=\"async\" class=\"size-full wp-image-4375 aligncenter\" src=\"http:\/\/www.neb-online.fr\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Deoxynucleotide Solutions, Mix<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">N0447L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">40 \u00b5mol each<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/N0447l\"><img decoding=\"async\" class=\"size-full wp-image-4375 aligncenter\" src=\"http:\/\/www.neb-online.fr\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator color=&#8221;custom&#8221; accent_color=&#8221;#919191&#8243;][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<em>Phusion\u00ae is a registered trademark and property of Thermo Fisher Scientific.\u00a0<\/em> <em>Phusion\u00ae DNA Polymerase was developed by Finnzymes Oy, now a part of Thermo Fisher Scientific.<\/em> <em>AccuPrime\u2122 is a trademark of Life Technologies, Inc.<\/em> <em>Platinum\u00ae is a registered trademark of Life Technologies, Inc.<\/em> <em>PfuUltra\u2122 is a trademark of Agilent Technologies, Inc.<\/em> <em>Q5\u00ae, Deep Vent\u2122 and 9\u00b0N\u2122 are trademarks of New England Biolabs, Inc.<\/em> <em>Vent\u2122 is a registered trademark of New England Biolabs, Inc.<\/em> <em>LabChip\u00ae is a registered trademark of Caliper Life Sciences, part of Perkin Elmer, Inc.<\/em>[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row][vc_column][vc_column_text][\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text] Fidelity at its finest [\/vc_column_text][\/vc_column][vc_column width=&#8221;2\/3&#8243; css=&#8221;.vc_custom_1429281526171{margin-bottom: 12px !important;}&#8221;][vc_column_text]Q5\u00ae High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available\u00a0(&gt;100 times higher than\u00a0Taq), Q5 DNA Polymerase results in ultra-low\u00a0error rates.\u00a0Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA&#8230;  <a class=\"excerpt-read-more\" href=\"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/high-fidelity-pcr\/q5-high-fidelity-dna-polymerase\/\" title=\"Read Q5 High Fidelity DNA Polymerase\">Read more &raquo;<\/a><\/p>\n","protected":false},"author":15,"featured_media":1648,"parent":6417,"menu_order":0,"comment_status":"open","ping_status":"open","template":"page-sidebar-pcr-dna-amplification.php","meta":{"footnotes":""},"class_list":["post-2109","page","type-page","status-publish","has-post-thumbnail","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.0 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Q5 High Fidelity DNA Polymerase - New England Biolabs France<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/high-fidelity-pcr\/q5-high-fidelity-dna-polymerase\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Q5 High Fidelity DNA Polymerase - New England Biolabs France\" \/>\n<meta property=\"og:description\" content=\"[vc_row][vc_column][vc_column_text][\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text] Fidelity at its finest [\/vc_column_text][\/vc_column][vc_column width=&#8221;2\/3&#8243; css=&#8221;.vc_custom_1429281526171{margin-bottom: 12px !important;}&#8221;][vc_column_text]Q5\u00ae High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available\u00a0(&gt;100 times higher than\u00a0Taq), Q5 DNA Polymerase results in ultra-low\u00a0error rates.\u00a0Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA... 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