{"id":2120,"date":"2015-04-27T13:45:31","date_gmt":"2015-04-27T13:45:31","guid":{"rendered":"http:\/\/www.neb-online.fr\/?page_id=2120"},"modified":"2020-04-16T15:58:26","modified_gmt":"2020-04-16T13:58:26","slug":"phusion-dna-polymerase","status":"publish","type":"page","link":"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/high-fidelity-pcr\/phusion-dna-polymerase\/","title":{"rendered":"Phusion DNA Polymerase"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row][vc_column][vc_column_text][\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;3\/4&#8243; css=&#8221;.vc_custom_1429044102012{margin-bottom: 40px !important;}&#8221;][vc_column_text]<\/p>\n<h2>Phusion\u00ae\u00a0Technology<\/h2>\n<p><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/M0530@\" target=\"_blank\" rel=\"noopener noreferrer\">Phusion DNA Polymerase<\/a> brings together a novel Pyrococcus-like enzyme with a processivity-enhancing domain and generates PCR products with accuracy and speed previously unattainable with a single enzyme, even on your most difficult templates. Additionally, Phusion DNA Polymerase is capable of amplifying long templates. In the quality control tests of Phusion DNA Polymerase, 7.5 kb genomic DNA and 20 kb \u03bb DNA are routinely amplified. The unique structure and characteristics of Phusion DNA Polymerase make it a superior choice for cloning. The Phusion High-Fidelity DNA Polymerase sets a new standard for PCR performance.[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/4&#8243; css=&#8221;.vc_custom_1429045798588{margin-bottom: 40px !important;}&#8221;][vc_column_text]<\/p>\n<p style=\"text-align: center;\"><strong>Try<\/strong><\/p>\n<p style=\"text-align: center;\"><strong><a title=\"Q5 High Fidelity DNA Polymerase\" href=\"http:\/\/www.neb-online.fr\/en\/neb\/pcr-and-dna-amplification\/high-fidelity-pcr\/q5-high-fidelity-dna-polymerase\/\">Q5\u00ae DNA Polymerase<\/a><\/strong><\/p>\n<p style=\"text-align: center;\"><strong>Higher fidelity and\u00a0faster extension times at the same price!<\/strong><\/p>\n<p>[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/2&#8243;][vc_column_text]<strong>Advantages:<\/strong><\/p>\n<ul>\n<li>Extreme Fidelity (50X greater than Taq)<\/li>\n<li>High Speed &#8211; Extension times are dramatically reduced (10X faster than Pfu)<\/li>\n<li>Robust Reactions &#8211; Maximal success with minimal optimization<\/li>\n<li>High Yield &#8211; Increased product yield with minimal enzyme amount<\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/2&#8243;][vc_column_text]<strong><a href=\"http:\/\/tmcalculator.neb.com\" target=\"_blank\" rel=\"noopener noreferrer\">NEB Tm Calculator:<\/a>\u00a0Determine your primers&#8217; optimal annealing temperature.<\/strong>[\/vc_column_text][vc_single_image image=&#8221;1088&#8243; img_size=&#8221;full&#8221; onclick=&#8221;custom_link&#8221; img_link_target=&#8221;_blank&#8221; link=&#8221;http:\/\/tmcalculator.neb.com&#8221;][\/vc_column][vc_column css=&#8221;.vc_custom_1429044170013{margin-top: 30px !important;}&#8221;][vc_column_text]<img fetchpriority=\"high\" decoding=\"async\" class=\" size-full wp-image-1085 alignleft\" src=\"http:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/04\/NEB_PHUSION_3.jpg\" alt=\"NEB_PHUSION_3\" width=\"470\" height=\"266\" srcset=\"https:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/04\/NEB_PHUSION_3.jpg 470w, https:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/04\/NEB_PHUSION_3-300x170.jpg 300w\" sizes=\"(max-width: 470px) 100vw, 470px\" \/><\/p>\n<div><\/div>\n<p><span class=\"bildunterschrift\"><em>3.8 kb fragment was amplified from 50 ng of Jurkat gDNA using different polymerases. Reactions were carried out according to the manufacter&#8217;s recommended conditions (cycling conditions are shown below). Extension times are indicated (in minutes)..\u00a0<a href=\"https:\/\/shop.neb-online.fr\/en\/art\/N3232@\" target=\"_blank\" rel=\"noopener noreferrer\">Ladder L is a 1 kb DNA Ladder #N3232.<\/a><\/em><\/span>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text][\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator color=&#8221;custom&#8221; accent_color=&#8221;#919191&#8243;][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<\/p>\n<h2>Available Products<\/h2>\n<p>[\/vc_column_text][vc_column_text css=&#8221;.vc_custom_1560949323945{margin-top: -20px !important;margin-bottom: 12px !important;}&#8221;]<\/p>\n<table style=\"width: 760px;\" border=\"0\" cellpadding=\"5\">\n<tbody>\n<tr style=\"background-color: #f3b281;\">\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"328\" height=\"40px\">PRODUCT<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"82\">REF.<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"131\">SIZE<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" colspan=\"1\">PRICE<\/th>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/M0530@\">Phusion\u00ae High-Fidelity DNA Polymerase<\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0530S\/L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100\/500 units<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"28\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/M0530@\"><img decoding=\"async\" class=\"aligncenter wp-image-4375 size-full\" src=\"https:\/\/www.neb-online.fr\/\/\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"NEB Shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/M0531@\">Phusion\u00ae High-Fidelity HF PCR Master Mix<\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0531S\/L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100\/500 rxn (\u00e0 50 \u00b5l volume)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/M0531@\"><img decoding=\"async\" class=\"aligncenter wp-image-4375 size-full\" src=\"http:\/\/www.neb-online.fr\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/M0532@\">Phusion\u00ae High-Fidelity GC PCR Master Mix<\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0532S\/L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100\/500 rxn (\u00e0 50 \u00b5l volume)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/M0532@\"><img decoding=\"async\" class=\"aligncenter wp-image-4375 size-full\" src=\"http:\/\/www.neb-online.fr\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/E0553@\">Phusion\u00ae High-Fidelity PCR Kit<\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E0553S\/L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">50\/200 units<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/E0553@\"><img decoding=\"async\" class=\"aligncenter wp-image-4375 size-full\" src=\"http:\/\/www.neb-online.fr\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; padding-top: 30px;\" colspan=\"5\"><strong>Phusion Hot Start Flex Polymerases :<\/strong><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/M0535@\">Phusion\u00ae Hot Start Flex DNA Polymerase<\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0535S\/L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100\/500 units<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/M0535@\"><img decoding=\"async\" class=\"aligncenter wp-image-4375 size-full\" src=\"http:\/\/www.neb-online.fr\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/M0536@\">Phusion\u00ae Hot Start Flex 2X Master Mix<\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0536S\/L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100\/500 units<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/M0536@\"><img decoding=\"async\" class=\"aligncenter wp-image-4375 size-full\" src=\"http:\/\/www.neb-online.fr\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; padding-top: 30px;\" colspan=\"5\"><strong>Companion products :<\/strong><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/N0447@\">Deoxynucleotide Solutions, Mix<\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">N0447S\/L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">8\u00a0\u00b5mol each\/ 40\u00a0\u00b5mol each<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/N0447@\"><img decoding=\"async\" class=\"aligncenter wp-image-4375 size-full\" src=\"http:\/\/www.neb-online.fr\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>[\/vc_column_text][vc_separator][vc_column_text]<\/p>\n<p class=\"p1\"><span class=\"s1\"><b>Legal notice<\/b><\/span><\/p>\n<p class=\"p1\"><span class=\"s2\">Licenses\/Patents\/Disclaimers:<\/span><\/p>\n<p class=\"p1\"><span class=\"s1\">Phusion\u00ae DNA Polymerase was developed by Finnzymes Oy, now a part of Thermo Fisher Scientific. This product is manufactured by New England Biolabs, Inc. under agreement with, and under the performance specifications of Thermo Fisher Scientific. Phusion\u00ae is a registered trademark and property of Thermo Fisher Scientific.<\/span><\/p>\n<p class=\"p1\"><span class=\"s2\">Notice to purchaser:\u00a0Limited license.<\/span><\/p>\n<p class=\"p1\"><span class=\"s1\">The purchase price of this product includes a limited, non-transferable license under U.S. and foreign patents owned by BIO-RAD Laboratories, Inc., to use this product. No other license under these patents is conveyed expressly or by implication to the purchaser by the purchase of this product.<\/span><\/p>\n<p class=\"p1\"><span class=\"s1\">The purchase price of this product includes a limited, non-transferable license under a patent owned by New England Biolabs, Inc., to use this product. No other license under this patent is conveyed expressly or by implication to the purchaser by the purchase of this product.<\/span><\/p>\n<p class=\"p1\"><span class=\"s1\">Nucleic acid-based aptamers for use with thermophilic DNA polymerases are licensed exclusively by New England Biolabs, Inc. from SomaLogic, Inc. (See Patent Nos. 5,475,096; 5,670,637; 5,696,249; 5,874,557; and 5,693,502). New England Biolabs, Inc. gives the Buyer\/User a non-exclusive license to use the aptamer-based Phusion Hot Start Flex DNA Polymerase for RESEARCH PURPOSES ONLY. Commercial use of the aptamer-based Phusion Hot Start Flex DNA Polymerase requires a license from New England Biolabs, Inc. Please contact\u00a0 busdev@neb.com\u00a0for more information.<\/span><\/p>\n<p>[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row][vc_column][vc_column_text][\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;3\/4&#8243; css=&#8221;.vc_custom_1429044102012{margin-bottom: 40px !important;}&#8221;][vc_column_text] Phusion\u00ae\u00a0Technology Phusion DNA Polymerase brings together a novel Pyrococcus-like enzyme with a processivity-enhancing domain and generates PCR products with accuracy and speed previously unattainable with a single enzyme, even on your most difficult templates. Additionally, Phusion DNA Polymerase is capable of amplifying long templates. In the quality control tests of&#8230;  <a class=\"excerpt-read-more\" href=\"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/high-fidelity-pcr\/phusion-dna-polymerase\/\" title=\"Read Phusion DNA Polymerase\">Read more &raquo;<\/a><\/p>\n","protected":false},"author":15,"featured_media":1406,"parent":6417,"menu_order":0,"comment_status":"open","ping_status":"closed","template":"page-sidebar-pcr-dna-amplification.php","meta":{"footnotes":""},"class_list":["post-2120","page","type-page","status-publish","has-post-thumbnail","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.0 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Phusion DNA Polymerase - New England Biolabs France<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/high-fidelity-pcr\/phusion-dna-polymerase\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Phusion DNA Polymerase - New England Biolabs France\" \/>\n<meta property=\"og:description\" content=\"[vc_row][vc_column][vc_column_text][\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;3\/4&#8243; css=&#8221;.vc_custom_1429044102012{margin-bottom: 40px !important;}&#8221;][vc_column_text] Phusion\u00ae\u00a0Technology Phusion DNA Polymerase brings together a novel Pyrococcus-like enzyme with a processivity-enhancing domain and generates PCR products with accuracy and speed previously unattainable with a single enzyme, even on your most difficult templates. Additionally, Phusion DNA Polymerase is capable of amplifying long templates. In the quality control tests of... Read more &raquo;\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/high-fidelity-pcr\/phusion-dna-polymerase\/\" \/>\n<meta property=\"og:site_name\" content=\"New England Biolabs France\" \/>\n<meta property=\"article:modified_time\" content=\"2020-04-16T13:58:26+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/04\/NEB_PCR_Header_dnaamplification_760px.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"760\" \/>\n\t<meta property=\"og:image:height\" content=\"200\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"3 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/high-fidelity-pcr\/phusion-dna-polymerase\/\",\"url\":\"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/high-fidelity-pcr\/phusion-dna-polymerase\/\",\"name\":\"Phusion DNA Polymerase - New England Biolabs France\",\"isPartOf\":{\"@id\":\"https:\/\/www.neb-online.fr\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/high-fidelity-pcr\/phusion-dna-polymerase\/#primaryimage\"},\"image\":{\"@id\":\"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/high-fidelity-pcr\/phusion-dna-polymerase\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/04\/NEB_PCR_Header_dnaamplification_760px.jpg\",\"datePublished\":\"2015-04-27T13:45:31+00:00\",\"dateModified\":\"2020-04-16T13:58:26+00:00\",\"breadcrumb\":{\"@id\":\"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/high-fidelity-pcr\/phusion-dna-polymerase\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/high-fidelity-pcr\/phusion-dna-polymerase\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/high-fidelity-pcr\/phusion-dna-polymerase\/#primaryimage\",\"url\":\"https:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/04\/NEB_PCR_Header_dnaamplification_760px.jpg\",\"contentUrl\":\"https:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/04\/NEB_PCR_Header_dnaamplification_760px.jpg\",\"width\":760,\"height\":200,\"caption\":\"NEB_PCR_Header\"},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/high-fidelity-pcr\/phusion-dna-polymerase\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"PCR and DNA Amplification\",\"item\":\"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"High Fidelity PCR\",\"item\":\"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/high-fidelity-pcr\/\"},{\"@type\":\"ListItem\",\"position\":3,\"name\":\"Phusion DNA Polymerase\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/www.neb-online.fr\/#website\",\"url\":\"https:\/\/www.neb-online.fr\/\",\"name\":\"New England Biolabs France\",\"description\":\"be INSPIRED, drive DISCOVERY, stay GENUINE\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/www.neb-online.fr\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Phusion DNA Polymerase - New England Biolabs France","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/high-fidelity-pcr\/phusion-dna-polymerase\/","og_locale":"en_US","og_type":"article","og_title":"Phusion DNA Polymerase - New England Biolabs France","og_description":"[vc_row][vc_column][vc_column_text][\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;3\/4&#8243; css=&#8221;.vc_custom_1429044102012{margin-bottom: 40px !important;}&#8221;][vc_column_text] Phusion\u00ae\u00a0Technology Phusion DNA Polymerase brings together a novel Pyrococcus-like enzyme with a processivity-enhancing domain and generates PCR products with accuracy and speed previously unattainable with a single enzyme, even on your most difficult templates. Additionally, Phusion DNA Polymerase is capable of amplifying long templates. In the quality control tests of... 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