{"id":2206,"date":"2015-04-28T08:37:50","date_gmt":"2015-04-28T08:37:50","guid":{"rendered":"http:\/\/www.neb-online.fr\/?page_id=2206"},"modified":"2022-11-07T17:44:00","modified_gmt":"2022-11-07T16:44:00","slug":"competent-cells","status":"publish","type":"page","link":"https:\/\/www.neb-online.fr\/en\/competent-cells\/","title":{"rendered":"Competent Cells"},"content":{"rendered":"

[vc_row][vc_column][vc_column_text]Cloning Strains<\/strong><\/p>\n

For cloning experiments choose from several high efficiency competent cell strains<\/a>. These\u00a0E. coli<\/em>\u00a0strains are T1 phage resistant and are\u00a0endA\u00a0<\/em>deficient for high quality plasmid preparations. Additionally, all competent cells from NEB are free of animal products.<\/p>\n

Yeast<\/strong><\/p>\n

NEB offers chemically competent\u00a0Kluyveromyces\u00a0<\/em>lactis<\/em><\/a>\u00a0cells and variants of this strain that have been tailored for specific protein expression needs. These cells are suitable for transformation with any of our linearized pKLAC series expression vectors.<\/p>\n

E. coli<\/em>\u00a0for Protein Expression<\/strong><\/p>\n

NEB offers several strains<\/a> with varying levels of expression control, each with T1 phage resistance and extremely high transformation efficiencies.[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator color=”custom” accent_color=”#919191″][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<\/p>\n

Competent\u00a0E. coli<\/em> –\u00a0Competitor Cross-Reference Tool<\/h2>\n
<\/div>\n

[\/vc_column_text][vc_column_text]Use this tool to select another company’s competent cell product and find out which NEB strain is compatible. Choose either the product name or catalog number from the available selection, and this tool will identify the recommended NEB product and its advantages. A link to the product page where you can also order the product is provided.
\nCompetitor Cross-Reference Tool<\/strong><\/a><\/p>\n

<\/div>\n

[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator color=”custom” accent_color=”#919191″][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=”.vc_custom_1439991969375{padding-top: 0px !important;}”]<\/p>\n

Properties Table<\/h2>\n

[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Strain
\nProperties<\/td>\n		NEB#<\/td>\nTransform
\nEfficiency
\n(cfu\/\u00b5g)(1)<\/sup><\/td>\n		Strain<\/td>\nT1
\nPhage
\nResistant<\/td>\n		Library
\nConstruc
\n-tion(2)<\/sup><\/b><\/td>\n		Cloning\/
\nSub-
\ncloning<\/b><\/td>\n		Blue\/
\nWhite
\nScreening<\/td>\n		laclq<\/sup><\/span><\/td>\nlysY<\/span><\/td>\nEndo-
\nnucleaseI
\nDeficient(3)<\/sup><\/td>\n		Protease(4)<\/sup>
\nDeficient<\/td>\n		F\u00b4<\/td>\nRecA\u00af<\/td>\nT7 RNA
\nPolyme
\n-rase<\/td>\n		Cytoplasmic
\ndisulfide bond
\nformation(5)<\/sup><\/td>\n		Drug
\nResistance(6)<\/sup><\/td>\n<\/tr>\n
NEB Turbo<\/a><\/td>\nC2984<\/td>\n1-3×109<\/sup><\/td>\nK12<\/td>\n\u221a<\/td>\n\u221a<\/td>\n\u221a<\/td>\n\u221a<\/td>\n\u221a<\/td>\n\u2013<\/td>\n\u221a<\/td>\n\u2013<\/td>\n\u221a<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u2013<\/td>\nnit<\/td>\n<\/tr>\n
NEB 5-alpha<\/a><\/td>\nC2987<\/td>\n1-3×109<\/sup><\/td>\nK12<\/td>\n\u221a<\/td>\n\u221a<\/td>\n\u221a<\/td>\n\u221a<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u221a<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u221a<\/td>\n\u2013<\/td>\n\u2013<\/td>\nnone<\/td>\n<\/tr>\n
NEB 5-alpha
\nF’Iq<\/sup><\/span><\/a><\/td>\n		C2992<\/td>\n1-3×109<\/sup><\/td>\nK12<\/td>\n\u221a<\/td>\n\u221a<\/td>\n\u221a<\/td>\n\u221a<\/td>\n\u221a<\/td>\n\u2013<\/td>\n\u221a<\/td>\n\u2013<\/td>\n\u221a<\/td>\n\u221a<\/td>\n\u2013<\/td>\n\u2013<\/td>\ntet<\/td>\n<\/tr>\n
NEB 10-beta<\/a><\/td>\nC3019<\/td>\n1-3×109<\/sup><\/td>\nK12<\/td>\n\u221a<\/td>\n\u221a<\/td>\n\u221a<\/td>\n\u221a<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u221a<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u221a<\/td>\n\u2013<\/td>\n\u2013<\/td>\nstr<\/td>\n<\/tr>\n
dam-\/dcm-<\/a><\/td>\nC2925<\/td>\n1-3×106<\/sup><\/td>\nK12<\/td>\n\u221a<\/td>\n\u221a<\/td>\n\u221a<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u221a<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u2013<\/td>\ncam, str, nit<\/td>\n<\/tr>\n
NEB Stable<\/a><\/td>\nC3040<\/td>\n1-3×109<\/sup><\/td>\nK12<\/td>\n\u221a<\/td>\n\u221a<\/td>\n\u221a<\/td>\n\u221a<\/td>\n\u221a<\/td>\n\u2013<\/td>\n\u221a<\/td>\n\u2013<\/td>\n\u221a<\/td>\n\u221a<\/td>\n\u2013<\/td>\n\u2013<\/td>\ntet, str<\/td>\n<\/tr>\n
<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
Protein Expression Strain Properties<\/span><\/strong><\/td>\n<\/tr>\n
NEB Express<\/a><\/td>\nC2523<\/td>\n0.6-1×109<\/sup><\/td>\nB<\/td>\n\u221a<\/td>\n\u221a<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u221a<\/td>\n\u221a<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u2013<\/td>\nnit<\/td>\n<\/tr>\n
NEB Express Iq<\/sup><\/span><\/a><\/td>\nC3037<\/td>\n0.6-1×109<\/sup><\/td>\nB<\/td>\n\u221a<\/td>\n\u221a<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u221a<\/td>\n\u2013<\/td>\n\u221a<\/td>\n\u221a<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u2013<\/td>\ncam, nit<\/td>\n<\/tr>\n
T7 Express<\/a><\/td>\nC2566<\/td>\n0.6-1×109<\/sup><\/td>\nB<\/td>\n\u221a<\/td>\n\u221a<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u221a<\/td>\n\u221a<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u221a<\/td>\n\u2013<\/td>\nnit<\/td>\n<\/tr>\n
T7 Express
\nlysY<\/span><\/a><\/td>\n		C3010<\/td>\n0.6-1×109<\/sup><\/td>\nB<\/td>\n\u221a<\/td>\n\u221a<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u221a<\/td>\n\u221a<\/td>\n\u221a<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u221a<\/td>\n\u2013<\/td>\ncam, nit<\/td>\n<\/tr>\n
T7 Express
\nlysY\/Iq<\/sup><\/span><\/a><\/td>\n		C3013<\/td>\n0.6-1×109<\/sup><\/td>\nB<\/td>\n\u221a<\/td>\n\u221a<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u221a<\/td>\n\u221a<\/td>\n\u221a<\/td>\n\u221a<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u221a<\/td>\n\u2013<\/td>\ncam, nit<\/td>\n<\/tr>\n
T7 Express
\nCrystal<\/a><\/td>\n		C3022<\/td>\n0.6-1×109<\/sup><\/td>\nB<\/td>\n\u221a<\/td>\n\u221a<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u221a<\/td>\n\u221a<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u221a<\/td>\n\u2013<\/td>\nnit<\/td>\n<\/tr>\n
SHuffle T7<\/a><\/td>\nC3026<\/td>\n1 x 106<\/sup><\/td>\nK12<\/td>\n\u221a<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u221a<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u221a<\/td>\n\u2013<\/td>\n\u221a<\/td>\n\u221a<\/td>\nstr, spec<\/td>\n<\/tr>\n
SHuffle
\nExpress<\/a><\/td>\n		C3028<\/td>\n1 x 107<\/sup><\/td>\nB<\/td>\n\u221a<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u221a<\/td>\n\u2013<\/td>\n\u221a<\/td>\n\u221a<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u221a<\/td>\nspec<\/td>\n<\/tr>\n
SHuffle T7
\nExpress<\/a><\/td>\n		C3029<\/td>\n1 x 107<\/sup><\/td>\nB<\/td>\n\u221a<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u221a<\/td>\n\u2013<\/td>\n\u221a<\/td>\n\u221a<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u221a<\/td>\n\u221a<\/td>\nspec(7)<\/sup><\/td>\n<\/tr>\n
SHuffle T7
\nExpress\u00a0lysY<\/span><\/a><\/td>\n		C3030<\/td>\n1 x 107<\/sup><\/td>\nB<\/td>\n\u221a<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u221a<\/td>\n\u221a<\/td>\n\u221a<\/td>\n\u221a<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u221a<\/td>\n\u221a<\/td>\ncam,
\nspec(7)<\/sup><\/td>\n<\/tr>\n
BL21<\/a><\/td>\nC2530<\/td>\n1-5×107<\/sup><\/td>\nB<\/td>\n\u221a<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u221a<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u2013<\/td>\n<\/tr>\n
BL21(DE3)<\/a><\/td>\nC2527<\/td>\n1-5×107<\/sup><\/td>\nB<\/td>\n\u221a<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u221a<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u221a<\/td>\n\u2013<\/td>\n\u2013<\/td>\n<\/tr>\n
NiCo21(DE3)<\/a><\/td>\nC2529<\/td>\n1-5×107<\/sup><\/td>\nB<\/td>\n\u221a<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u221a<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u221a<\/td>\n\u2013<\/td>\n\u2013<\/td>\n<\/tr>\n
Lemo21(DE3)<\/a><\/td>\nC2528<\/td>\n1-3×107<\/sup><\/td>\nB<\/td>\n\u221a<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u221a<\/td>\n\u2013<\/td>\n\u221a<\/td>\n\u2013<\/td>\n\u2013<\/td>\n\u221a<\/td>\n\u2013<\/td>\ncam<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"

[vc_row][vc_column][vc_column_text]Cloning Strains For cloning experiments choose from several high efficiency competent cell strains. These\u00a0E. coli\u00a0strains are T1 phage resistant and are\u00a0endA\u00a0deficient for high quality plasmid preparations. Additionally, all competent cells from NEB are free of animal products. Yeast NEB offers chemically competent\u00a0Kluyveromyces\u00a0lactis\u00a0cells and variants of this strain that have been tailored for specific protein expression… Read more »<\/a><\/p>\n","protected":false},"author":15,"featured_media":9893,"parent":0,"menu_order":0,"comment_status":"open","ping_status":"open","template":"page-sidebar-competent-cells.php","meta":{"footnotes":""},"class_list":["post-2206","page","type-page","status-publish","has-post-thumbnail","hentry"],"yoast_head":"\nCompetent Cells - New England Biolabs France<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.neb-online.fr\/en\/competent-cells\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Competent Cells - New England Biolabs France\" \/>\n<meta property=\"og:description\" content=\"[vc_row][vc_column][vc_column_text]Cloning Strains For cloning experiments choose from several high efficiency competent cell strains. These\u00a0E. coli\u00a0strains are T1 phage resistant and are\u00a0endA\u00a0deficient for high quality plasmid preparations. Additionally, all competent cells from NEB are free of animal products. Yeast NEB offers chemically competent\u00a0Kluyveromyces\u00a0lactis\u00a0cells and variants of this strain that have been tailored for specific protein expression... Read more »\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.neb-online.fr\/en\/competent-cells\/\" \/>\n<meta property=\"og:site_name\" content=\"New England Biolabs France\" \/>\n<meta property=\"article:modified_time\" content=\"2022-11-07T16:44:00+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.neb-online.fr\/wp-content\/uploads\/FR_Article_Banner_CompCellBee.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"760\" \/>\n\t<meta property=\"og:image:height\" content=\"200\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"3 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.neb-online.fr\/en\/competent-cells\/\",\"url\":\"https:\/\/www.neb-online.fr\/en\/competent-cells\/\",\"name\":\"Competent Cells - New England Biolabs France\",\"isPartOf\":{\"@id\":\"https:\/\/www.neb-online.fr\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\/\/www.neb-online.fr\/en\/competent-cells\/#primaryimage\"},\"image\":{\"@id\":\"https:\/\/www.neb-online.fr\/en\/competent-cells\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/www.neb-online.fr\/wp-content\/uploads\/FR_Article_Banner_CompCellBee.jpg\",\"datePublished\":\"2015-04-28T08:37:50+00:00\",\"dateModified\":\"2022-11-07T16:44:00+00:00\",\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/www.neb-online.fr\/en\/competent-cells\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/www.neb-online.fr\/en\/competent-cells\/#primaryimage\",\"url\":\"https:\/\/www.neb-online.fr\/wp-content\/uploads\/FR_Article_Banner_CompCellBee.jpg\",\"contentUrl\":\"https:\/\/www.neb-online.fr\/wp-content\/uploads\/FR_Article_Banner_CompCellBee.jpg\",\"width\":760,\"height\":200},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/www.neb-online.fr\/#website\",\"url\":\"https:\/\/www.neb-online.fr\/\",\"name\":\"New England Biolabs France\",\"description\":\"be INSPIRED, drive DISCOVERY, stay GENUINE\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/www.neb-online.fr\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Competent Cells - New England Biolabs France","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.neb-online.fr\/en\/competent-cells\/","og_locale":"en_US","og_type":"article","og_title":"Competent Cells - New England Biolabs France","og_description":"[vc_row][vc_column][vc_column_text]Cloning Strains For cloning experiments choose from several high efficiency competent cell strains. These\u00a0E. coli\u00a0strains are T1 phage resistant and are\u00a0endA\u00a0deficient for high quality plasmid preparations. Additionally, all competent cells from NEB are free of animal products. Yeast NEB offers chemically competent\u00a0Kluyveromyces\u00a0lactis\u00a0cells and variants of this strain that have been tailored for specific protein expression... Read more »","og_url":"https:\/\/www.neb-online.fr\/en\/competent-cells\/","og_site_name":"New England Biolabs France","article_modified_time":"2022-11-07T16:44:00+00:00","og_image":[{"width":760,"height":200,"url":"https:\/\/www.neb-online.fr\/wp-content\/uploads\/FR_Article_Banner_CompCellBee.jpg","type":"image\/jpeg"}],"twitter_misc":{"Est. reading time":"3 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/www.neb-online.fr\/en\/competent-cells\/","url":"https:\/\/www.neb-online.fr\/en\/competent-cells\/","name":"Competent Cells - New England Biolabs France","isPartOf":{"@id":"https:\/\/www.neb-online.fr\/#website"},"primaryImageOfPage":{"@id":"https:\/\/www.neb-online.fr\/en\/competent-cells\/#primaryimage"},"image":{"@id":"https:\/\/www.neb-online.fr\/en\/competent-cells\/#primaryimage"},"thumbnailUrl":"https:\/\/www.neb-online.fr\/wp-content\/uploads\/FR_Article_Banner_CompCellBee.jpg","datePublished":"2015-04-28T08:37:50+00:00","dateModified":"2022-11-07T16:44:00+00:00","inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/www.neb-online.fr\/en\/competent-cells\/"]}]},{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/www.neb-online.fr\/en\/competent-cells\/#primaryimage","url":"https:\/\/www.neb-online.fr\/wp-content\/uploads\/FR_Article_Banner_CompCellBee.jpg","contentUrl":"https:\/\/www.neb-online.fr\/wp-content\/uploads\/FR_Article_Banner_CompCellBee.jpg","width":760,"height":200},{"@type":"WebSite","@id":"https:\/\/www.neb-online.fr\/#website","url":"https:\/\/www.neb-online.fr\/","name":"New England Biolabs France","description":"be INSPIRED, drive DISCOVERY, stay GENUINE","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/www.neb-online.fr\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"}]}},"_links":{"self":[{"href":"https:\/\/www.neb-online.fr\/en\/wp-json\/wp\/v2\/pages\/2206","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.neb-online.fr\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.neb-online.fr\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.neb-online.fr\/en\/wp-json\/wp\/v2\/users\/15"}],"replies":[{"embeddable":true,"href":"https:\/\/www.neb-online.fr\/en\/wp-json\/wp\/v2\/comments?post=2206"}],"version-history":[{"count":1,"href":"https:\/\/www.neb-online.fr\/en\/wp-json\/wp\/v2\/pages\/2206\/revisions"}],"predecessor-version":[{"id":24797,"href":"https:\/\/www.neb-online.fr\/en\/wp-json\/wp\/v2\/pages\/2206\/revisions\/24797"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.neb-online.fr\/en\/wp-json\/wp\/v2\/media\/9893"}],"wp:attachment":[{"href":"https:\/\/www.neb-online.fr\/en\/wp-json\/wp\/v2\/media?parent=2206"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}