{"id":2213,"date":"2015-04-28T08:58:40","date_gmt":"2015-04-28T08:58:40","guid":{"rendered":"http:\/\/www.neb-online.fr\/?page_id=2213"},"modified":"2022-08-02T15:22:17","modified_gmt":"2022-08-02T13:22:17","slug":"cloning-strains","status":"publish","type":"page","link":"https:\/\/www.neb-online.fr\/en\/competent-cells\/cloning-strains\/","title":{"rendered":"Cloning Strains"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row][vc_column width=&#8221;2\/3&#8243;][vc_column_text]Ensure successful transformations with NEB competent\u00a0<em>E. coli<\/em>\u00a0for cloning. Choose from several high efficiency competent cell strains in a variety of formats that deliver performance, convenience and value.<\/p>\n<p>NEB Competent\u00a0<em>E. coli<\/em>\u00a0\u2013 building a strong foundation for your cloning experiments.[\/vc_column_text]<div class=\"vbox\">\n\t\t\t\t<span class=\"vhead\">Advantages:<\/span>\n\t\t\t\t\t<ul class=\"vlist\">\n\t\t\t\t\t\t<li><span>High transformation efficiencies<br \/><\/span><\/li><li><span>EndA deficient for high quality plasmid preparation<br \/><\/span><\/li><li><span>T1 phage resistant<br \/><\/span><\/li><li><span>RecA deficient for reduced recombination of cloned DNA<br \/><\/span><\/li><li><span>Free of animal products<br \/><\/span><\/li><li><span>Suitable for blue\/white screening<br \/><\/span><\/li><li><span>Available as single-use tubes or multiple use aliquots<br \/><\/span><\/li><li><span>Outgrowth media and control plasmid included<br \/><\/span><\/li><li><span>Bulk sales capabilities with custom packaging formats<\/span><\/li>\n\t\t\t\t\t<\/ul>\n\t\t\t<\/div>[\/vc_column][vc_column width=&#8221;1\/3&#8243;][vc_single_image image=&#8221;19627&#8243; img_size=&#8221;full&#8221; alignment=&#8221;center&#8221; onclick=&#8221;custom_link&#8221; link=&#8221;https:\/\/www.neb-online.fr\/wp-content\/uploads\/NEB_Competent_Cells.pdf&#8221;][vc_column_text]<\/p>\n<p style=\"text-align: center;\"><strong>Competent Cells<\/strong><\/p>\n<p>[\/vc_column_text]<a class=\"btn btn-standard form-btn\"  href=\"https:\/\/www.neb-online.fr\/en\/doc-request\/\" style=\"margin-right:auto;\">Order Brochure<\/a>[\/vc_column][\/vc_row][vc_row][vc_column css=&#8221;.vc_custom_1429216682214{margin-bottom: 22px !important;}&#8221;][vc_separator color=&#8221;custom&#8221; accent_color=&#8221;#919191&#8243;][vc_column_text css=&#8221;.vc_custom_1438182120778{margin-bottom: 6px !important;}&#8221;]<\/p>\n<h3>NEB 5-alpha<\/h3>\n<p><a href=\"https:\/\/shop.neb-online.fr\/en\/arts\/C2987H;C2987P;C2987I;C2988J;C2989K\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"alignright size-full wp-image-1225\" src=\"http:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/04\/NEB_Shop_icon1.png\" alt=\"NEB_Shop_icon\" width=\"50\" height=\"50\" \/><\/a>[\/vc_column_text][vc_column_text]A versatile\u00a0<em>E. coli<\/em>\u00a0strain, NEB 5-alpha is a derivative of DH5\u03b1\u2122, and has the same genetic features of this popular cloning strain. NEB 5-alpha offers high transformation efficiencies, convenient formats and value pricing. Whether you are doing routine cloning, subcloning or looking for a high efficiency (electrocompetent) format, NEB 5-alpha is the ideal strain for you.[\/vc_column_text][vc_separator color=&#8221;custom&#8221; accent_color=&#8221;#919191&#8243;][vc_column_text]<\/p>\n<h3>NEB 10-Beta<\/h3>\n<p><a href=\"https:\/\/shop.neb-online.fr\/en\/arts\/C3019H;C3019I;C3020K\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"alignright size-full wp-image-1225\" src=\"http:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/04\/NEB_Shop_icon1.png\" alt=\"NEB_Shop_icon\" width=\"50\" height=\"50\" \/><\/a>[\/vc_column_text][vc_column_text]NEB 10-beta is a DH10B\u2122 derivative that can be used for routine cloning, but is ideally suited for the cloning of large plasmids and BACs. NEB 10-beta also offers high transformation efficiencies, convenient formats and value pricing.[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column css=&#8221;.vc_custom_1429216706894{margin-bottom: 22px !important;}&#8221;][vc_separator color=&#8221;custom&#8221; accent_color=&#8221;#919191&#8243;][vc_column_text]<\/p>\n<h3>dam-\/dcm- Competent\u00a0<em>E. coli<\/em><\/h3>\n<p><strong><a href=\"https:\/\/shop.neb-online.fr\/en\/arts\/C2925H;C2925I\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"alignright size-full wp-image-1225\" src=\"http:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/04\/NEB_Shop_icon1.png\" alt=\"NEB_Shop_icon\" width=\"50\" height=\"50\" \/><\/a><\/strong>[\/vc_column_text][vc_column_text]Dam\/Dcm methyltransferase free plasmid growth[\/vc_column_text][vc_separator color=&#8221;custom&#8221; accent_color=&#8221;#919191&#8243;][vc_column_text]<\/p>\n<h3>NEB Turbo<\/h3>\n<p><strong><a href=\"https:\/\/shop.neb-online.fr\/en\/arts\/C2984H;C2984I;C2986K\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"alignright size-full wp-image-1225\" src=\"http:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/04\/NEB_Shop_icon1.png\" alt=\"NEB_Shop_icon\" width=\"50\" height=\"50\" \/><\/a><\/strong>[\/vc_column_text][vc_column_text]With colonies visible in 6.5 hours, NEB Turbo couples fast colony growth and tight control of expression. Available as high efficiency chemical competent or electrocompetent cells. NEB Turbo can used for the cloning\/expression of toxic genes.[\/vc_column_text][vc_separator color=&#8221;custom&#8221; accent_color=&#8221;#919191&#8243;][vc_column_text]<\/p>\n<h3>NEB Stable Competent E. coli<\/h3>\n<p><strong><a href=\"https:\/\/shop.neb-online.fr\/en\/arts\/C3040H;C3040I\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"alignright size-full wp-image-1225\" src=\"http:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/04\/NEB_Shop_icon1.png\" alt=\"NEB_Shop_icon\" width=\"50\" height=\"50\" \/><\/a><\/strong>[\/vc_column_text][vc_column_text]<\/p>\n<ul>\n<li>Transformation efficiency: 1-3 x 10<sup>9<\/sup> cfu\/\u00b5g pUC19 DNA<\/li>\n<li>Recommended host strain for cloning genes into retroviral\/ lentiviral vectors<\/li>\n<li>Recommended for cloning of direct repeats and inverted repeat sequences<\/li>\n<li>Reduced recombination of cloned DNA (recA1)<\/li>\n<li>Efficient transformation of methylated DNA derived from eukaryotic sources or unmethylated DNA derived from PCR, cDNA and many other sources [mcrA\u0394(mrr-hsdRMS-mcrBC)]<\/li>\n<li>Activity of nonspecific endonuclease I (endA1) eliminated for highest quality plasmid preparations<\/li>\n<li>Resistance to phage T1 (fhuA)<\/li>\n<li>Suitable for blue\/white screening with vectors capable of alpha-complementation<\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][vc_column_text]<\/p>\n<h2>Available products<\/h2>\n<table style=\"width: 760px;\" border=\"0\" cellpadding=\"5\">\n<tbody>\n<tr style=\"background-color: #f3b281;\">\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"350\" height=\"40px\">PRODUCT<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"80\">REF.<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"200\">SIZE<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" colspan=\"1\" width=\"130\">PRICE<\/th>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"5\">NEB 5-alpha Competent <em>E. coli<\/em><\/td>\n<td style=\"text-align: center; border-bottom: 1px solid #eb7e2d;\">C2987H<\/td>\n<td style=\"text-align: center; border-bottom: 1px solid #eb7e2d;\">20 x 0,05 ml (20 rxn)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/c2987H\"><img decoding=\"async\" class=\"size-full wp-image-4375 aligncenter\" src=\"http:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center; border-bottom: 1px solid #eb7e2d;\">C2987I<\/td>\n<td style=\"text-align: center; border-bottom: 1px solid #eb7e2d;\">6 x 0,2 ml (24 rxn)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/c2987I\"><img decoding=\"async\" class=\"size-full wp-image-4375 aligncenter\" src=\"http:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center; border-bottom: 1px solid #eb7e2d;\">C2987P<\/td>\n<td style=\"text-align: center; border-bottom: 1px solid #eb7e2d;\">96-well (20\u00b5l each)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/c2987P\"><img decoding=\"async\" class=\"size-full wp-image-4375 aligncenter\" src=\"http:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center; border-bottom: 1px solid #eb7e2d;\">C2987U<\/td>\n<td style=\"text-align: center; border-bottom: 1px solid #eb7e2d;\">96 x 50\u00b5l<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/c2987U\"><img decoding=\"async\" class=\"size-full wp-image-4375 aligncenter\" src=\"http:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">C2987R<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">1\u00d7384 wells (10\u00b5l each)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/c2987R\"><img decoding=\"async\" class=\"size-full wp-image-4375 aligncenter\" src=\"http:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">NEB 5-alpha Electrocompetent <em>E. coli<\/em><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">C2989K<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">6 x 0,1 ml (24 rxn)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/c2989K\"><img decoding=\"async\" class=\"size-full wp-image-4375 aligncenter\" src=\"http:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">NEB 5-alpha Competent <em>E. coli<\/em> (Subcloning efficiency)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">C2988J<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">6 x 0,4 ml (48 rxn)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/c2988J\"><img decoding=\"async\" class=\"size-full wp-image-4375 aligncenter\" src=\"http:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"2\">NEB 5-alpha F\u00b4 I q Competent <em>E. coli<\/em><\/td>\n<td style=\"text-align: center; border-bottom: 1px solid #eb7e2d;\">C2992H<\/td>\n<td style=\"text-align: center; border-bottom: 1px solid #eb7e2d;\">20 x 0,05 ml (20 rxn)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/c2992H\"><img decoding=\"async\" class=\"size-full wp-image-4375 aligncenter\" src=\"http:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">C2992I<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">6 x 0,2 ml (24 rxn)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/c2992I\"><img decoding=\"async\" class=\"size-full wp-image-4375 aligncenter\" src=\"http:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"2\">NEB 10-beta Competent <em>E. coli<\/em><\/td>\n<td style=\"text-align: center; border-bottom: 1px solid #eb7e2d;\">C3019H<\/td>\n<td style=\"text-align: center; border-bottom: 1px solid #eb7e2d;\">20 x 0,05 ml (20 rxn)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/c3019H\"><img decoding=\"async\" class=\"size-full wp-image-4375 aligncenter\" src=\"http:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">C3019I<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">6 x 0,2 ml (24 rxn)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/c3019I\"><img decoding=\"async\" class=\"size-full wp-image-4375 aligncenter\" src=\"http:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">NEB 10-beta Electrocompetent <em>E. coli<\/em><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">C3020K<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">6 x 0,1 ml (24 rxn)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/c3020K\"><img decoding=\"async\" class=\"size-full wp-image-4375 aligncenter\" src=\"http:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"2\">NEB Turbo Competent <em>E. coli<\/em><\/td>\n<td style=\"text-align: center; border-bottom: 1px solid #eb7e2d;\">C2984H<\/td>\n<td style=\"text-align: center; border-bottom: 1px solid #eb7e2d;\">20 x 0,05 ml (20 rxn)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/c2984H\"><img decoding=\"async\" class=\"size-full wp-image-4375 aligncenter\" src=\"http:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">C2984I<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">6 x 0,2 ml (24 rxn)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/c2984I\"><img decoding=\"async\" class=\"size-full wp-image-4375 aligncenter\" src=\"http:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">NEB Turbo Electrocompetent <em>E. coli<\/em><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">C2986K<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">6 x 0,1 ml (24 rxn)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/c2986K\"><img decoding=\"async\" class=\"size-full wp-image-4375 aligncenter\" src=\"http:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"2\">NEB Stable Competent <em>E. coli<\/em><\/td>\n<td style=\"text-align: center; border-bottom: 1px solid #eb7e2d;\">C3040H<\/td>\n<td style=\"text-align: center; border-bottom: 1px solid #eb7e2d;\">20 x 0,05 ml (20 rxn)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/c3040H\"><img decoding=\"async\" class=\"size-full wp-image-4375 aligncenter\" src=\"http:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">C3040I<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">6 x 0,2 ml (24 rxn)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/c3040I\"><img decoding=\"async\" class=\"size-full wp-image-4375 aligncenter\" src=\"http:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row][vc_column width=&#8221;2\/3&#8243;][vc_column_text]Ensure successful transformations with NEB competent\u00a0E. coli\u00a0for cloning. Choose from several high efficiency competent cell strains in a variety of formats that deliver performance, convenience and value. NEB Competent\u00a0E. coli\u00a0\u2013 building a strong foundation for your cloning experiments.[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/3&#8243;][vc_single_image image=&#8221;19627&#8243; img_size=&#8221;full&#8221; alignment=&#8221;center&#8221; onclick=&#8221;custom_link&#8221; link=&#8221;https:\/\/www.neb-online.fr\/wp-content\/uploads\/NEB_Competent_Cells.pdf&#8221;][vc_column_text] Competent Cells [\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column css=&#8221;.vc_custom_1429216682214{margin-bottom: 22px !important;}&#8221;][vc_separator color=&#8221;custom&#8221; accent_color=&#8221;#919191&#8243;][vc_column_text css=&#8221;.vc_custom_1438182120778{margin-bottom: 6px&#8230;  <a class=\"excerpt-read-more\" href=\"https:\/\/www.neb-online.fr\/en\/competent-cells\/cloning-strains\/\" title=\"Read Cloning Strains\">Read more &raquo;<\/a><\/p>\n","protected":false},"author":15,"featured_media":9893,"parent":2206,"menu_order":0,"comment_status":"open","ping_status":"open","template":"page-sidebar-competent-cells.php","meta":{"footnotes":""},"class_list":["post-2213","page","type-page","status-publish","has-post-thumbnail","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.0 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Cloning Strains - New England Biolabs France<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.neb-online.fr\/en\/competent-cells\/cloning-strains\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Cloning Strains - New England Biolabs France\" \/>\n<meta property=\"og:description\" content=\"[vc_row][vc_column width=&#8221;2\/3&#8243;][vc_column_text]Ensure successful transformations with NEB competent\u00a0E. coli\u00a0for cloning. Choose from several high efficiency competent cell strains in a variety of formats that deliver performance, convenience and value. NEB Competent\u00a0E. coli\u00a0\u2013 building a strong foundation for your cloning experiments.[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/3&#8243;][vc_single_image image=&#8221;19627&#8243; img_size=&#8221;full&#8221; alignment=&#8221;center&#8221; onclick=&#8221;custom_link&#8221; link=&#8221;https:\/\/www.neb-online.fr\/wp-content\/uploads\/NEB_Competent_Cells.pdf&#8221;][vc_column_text] Competent Cells [\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column css=&#8221;.vc_custom_1429216682214{margin-bottom: 22px !important;}&#8221;][vc_separator color=&#8221;custom&#8221; accent_color=&#8221;#919191&#8243;][vc_column_text css=&#8221;.vc_custom_1438182120778{margin-bottom: 6px... 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