{"id":2236,"date":"2015-04-29T07:47:04","date_gmt":"2015-04-29T07:47:04","guid":{"rendered":"http:\/\/www.neb-online.fr\/?page_id=2236"},"modified":"2022-07-29T09:13:20","modified_gmt":"2022-07-29T07:13:20","slug":"glycosidases","status":"publish","type":"page","link":"https:\/\/www.neb-online.fr\/en\/glycobiology\/glycosidases\/","title":{"rendered":"Glycosidases"},"content":{"rendered":"

[vc_row][vc_column width=”2\/3″][vc_column_text]New England Biolabs (NEB) offers a selection of endoglycosidases, exoglycosidases, and heparinases for glycobiology research. Many of these reagents are recombinant, and all undergo several quality control assays, enabling us to provide products with lower unit cost, high purity and reduced lot-to-lot variation. All of our glycosidases are tested for contaminants. Since p-nitrophenyl-glycosides are not hydrolyzed by some exoglycosidases, we use only fluorescently-labeled oligosaccharides to screen for contaminating glycosidases.<\/p>\n

And, to simplify workflows and digestions with two or more enzymes, the number of glycosidase reaction buffers<\/a> was recently decreased from 6 to 3.[\/vc_column_text][\/vc_column][vc_column width=”1\/3″][vc_column_text]<\/p>\n

Glycobiology Brochure<\/strong><\/p>\n

<\/del>\"Cover<\/a><\/p>\n

[\/vc_column_text]Order Brochure<\/a>[\/vc_column][\/vc_row][vc_row][vc_column][vc_separator color=”black”][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<\/p>\n

Remove-iT\u2122 Endoglycosidases<\/h2>\n

[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column width=”2\/3″][vc_column_text]Remove-iT PNGase F<\/a><\/strong> is an amidase which cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides from\u00a0N<\/em>-linked glycoproteins. Remove-iT PNGase F is tagged with a chitin binding domain (CBD) for easy removal from a reaction<\/a> and is supplied glycerol free for optimal performance in HPLC and MS intensive methods.<\/p>\n

\"specificity_pngasef\"<\/a>[\/vc_column_text][\/vc_column][vc_column width=”1\/3″][vc_column_text]<\/p>\n

Application Note<\/strong><\/p>\n

Remove-iT\u00ae PNGase F: Effective Release and Recovery of Neutral and Sialylated N<\/em>-glycans<\/p>\n

\"cover_removeit_appnote\"<\/a>[\/vc_column_text]Download PDF<\/a>[\/vc_column][\/vc_row][vc_row][vc_column width=”1\/2″ css=”.vc_custom_1429143713369{margin-bottom: 30px !important;}”][vc_column_text]Remove-iT Endo S<\/a><\/strong> is an endoglycosidase with a uniquely high specificity for removing\u00a0N<\/em>-linked glycans from the chitobiose core of the heavy chain of native IgG. Remove-iT Endo S is tagged with a chitin binding domain (CBD) for easy removal from a reaction<\/a> and is supplied glycerol free for optimal performance in HPLC and MS intensive methods.\"specificity_endos\"<\/a>[\/vc_column_text][\/vc_column][vc_column width=”1\/2″ css=”.vc_custom_1429143716867{margin-bottom: 30px !important;}”][vc_column_text]Remove-iT Endo D<\/a><\/strong> , also known as Endoglycosidase D, is a recombinant glycosidase, which cleaves within the chitobiose core of paucimannose N-linked glycans, with or without extensions in the antennae. Remove-iT Endo D is tagged with a chitin binding domain (CBD) for easy removal from a reaction<\/a>, and is supplied glycerol-free for optimal performance in HPLC and MS- intensive methods.<\/p>\n

\"specificity_endod\"<\/a>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator color=”custom” accent_color=”#919191″][\/vc_column][\/vc_row][vc_row][vc_column width=”1\/2″][vc_column_text]<\/p>\n

Protein Deglycosylation Mix<\/h2>\n

The\u00a0Protein Deglycosylation Mix<\/a>\u00a0kit contains all of the enzymes, reagents, and controls needed to remove almost all\u00a0N<\/em>-linked and simple\u00a0O<\/em>-linked glycans as well as some complex\u00a0O<\/em>-linked glycans. This kit contains enzyme sufficient for 20 reactions or the cleavage of as much as 2 mg of glycoprotein.[\/vc_column_text][\/vc_column][vc_column width=”1\/2″ css=”.vc_custom_1429143732706{margin-bottom: 30px !important;}”][vc_column_text]N<\/em>– and\u00a0O<\/em>-Glycosylation<\/strong>\"NEB_GLYCO_image1\" \"NEB_GLYCO_image1_legende\"[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]Visit our shop<\/a>\u00a0to view our full range of Glycobiology products.[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"

[vc_row][vc_column width=”2\/3″][vc_column_text]New England Biolabs (NEB) offers a selection of endoglycosidases, exoglycosidases, and heparinases for glycobiology research. Many of these reagents are recombinant, and all undergo several quality control assays, enabling us to provide products with lower unit cost, high purity and reduced lot-to-lot variation. All of our glycosidases are tested for contaminants. Since p-nitrophenyl-glycosides are… Read more »<\/a><\/p>\n","protected":false},"author":15,"featured_media":1680,"parent":2233,"menu_order":0,"comment_status":"open","ping_status":"open","template":"page-sidebar-glycobiology.php","meta":{"footnotes":""},"class_list":["post-2236","page","type-page","status-publish","has-post-thumbnail","hentry"],"yoast_head":"\nGlycosidases - New England Biolabs France<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.neb-online.fr\/en\/glycobiology\/glycosidases\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Glycosidases - New England Biolabs France\" \/>\n<meta property=\"og:description\" content=\"[vc_row][vc_column width=”2\/3″][vc_column_text]New England Biolabs (NEB) offers a selection of endoglycosidases, exoglycosidases, and heparinases for glycobiology research. 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