{"id":2242,"date":"2015-04-29T07:51:23","date_gmt":"2015-04-29T07:51:23","guid":{"rendered":"http:\/\/www.neb-online.fr\/?page_id=2242"},"modified":"2020-04-11T20:32:54","modified_gmt":"2020-04-11T18:32:54","slug":"rapid-pngase-f","status":"publish","type":"page","link":"https:\/\/www.neb-online.fr\/en\/glycobiology\/rapid-pngase-f\/","title":{"rendered":"Rapid PNGase F"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row][vc_column][vc_column_text]<\/p>\n<div class=\"page\" title=\"Page 1\">\n<div class=\"section\">\n<div class=\"layoutArea\">\n<div class=\"column\">\n<p>PNGase F is the most effective enzymatic method for removing almost all N-linked oligosaccharides from glycoproteins. PNGase F digestion deaminates the asparagine residue to aspartic acid, and leaves the oligosaccharide intact, keeping it suitable for further analysis.<\/p>\n<p><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/p0710S\" target=\"_blank\" rel=\"noopener noreferrer\">Rapid PNGase F<\/a> is an improved reagent that allows the complete and rapid deglycosylation of antibodies and immunoglobulin fusion proteins, as well as other glycoproteins, in minutes. All N-glycans are released rapidly and without bias, and are ready to\u00a0be prepared for downstream chromatography or mass spectrometry analysis. Rapid PNGase F creates an optimized workflow, reducing processing time without compromising sensitivity or reproducibility.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]<strong>Advantages:<\/strong><\/p>\n<ul>\n<li>Complete deglycosylation of antibodies and\u00a0immunoglobulin fusion proteins in minutes<\/li>\n<li>Release of all N-glycans rapidly and without bias, ready for downstream chromatography or mass spectrometry analysis<\/li>\n<li>Recombinant source<\/li>\n<li>Optimal activity is ensured for 12 months<\/li>\n<li>Purified to &gt;99% homogeneity, as determined by SDS-PAGE<\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/2&#8243;][vc_column_text]<span class=\"bildunterschrift\"><img fetchpriority=\"high\" decoding=\"async\" class=\"alignnone size-full wp-image-1229\" src=\"http:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/04\/graph_rapidpngasef.jpg\" alt=\"graph_rapidpngasef\" width=\"389\" height=\"356\" srcset=\"https:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/04\/graph_rapidpngasef.jpg 389w, https:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/04\/graph_rapidpngasef-300x275.jpg 300w\" sizes=\"(max-width: 389px) 100vw, 389px\" \/>\u00a0<\/span>[\/vc_column_text][vc_column_text]<span class=\"bildunterschrift\">ESI-TOF analysis of an antibody before and after treatment with Rapid PNGase F.<\/span>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<\/p>\n<h2>Available product<\/h2>\n<table style=\"width: 760px;\" border=\"0\" cellpadding=\"5\">\n<tbody>\n<tr style=\"background-color: #f3f3f3; font-size: 16px;\">\n<th style=\"border-bottom: solid #eb7e2d;\" align=\"left\" width=\"400\" height=\"40px\">PRODUCT<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"88\">REF.<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"100\">SIZE<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" colspan=\"1\">PRICE<\/th>\n<\/tr>\n<tr style=\"background-color: #ffffff;\">\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"1\">Rapid PNGase F<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">P0710 S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">50 rxn<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/P0710S\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p style=\"text-align: right;\"><span class=\"bildunterschrift\">Au 02.01.2018<\/span><\/p>\n<p>[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row][vc_column][vc_column_text] PNGase F is the most effective enzymatic method for removing almost all N-linked oligosaccharides from glycoproteins. PNGase F digestion deaminates the asparagine residue to aspartic acid, and leaves the oligosaccharide intact, keeping it suitable for further analysis. Rapid PNGase F is an improved reagent that allows the complete and rapid deglycosylation of antibodies and&#8230;  <a class=\"excerpt-read-more\" href=\"https:\/\/www.neb-online.fr\/en\/glycobiology\/rapid-pngase-f\/\" title=\"Read Rapid PNGase F\">Read more &raquo;<\/a><\/p>\n","protected":false},"author":15,"featured_media":1680,"parent":2233,"menu_order":0,"comment_status":"open","ping_status":"open","template":"page-sidebar-glycobiology.php","meta":{"footnotes":""},"class_list":["post-2242","page","type-page","status-publish","has-post-thumbnail","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.0 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Rapid PNGase F - New England Biolabs France<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.neb-online.fr\/en\/glycobiology\/rapid-pngase-f\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Rapid PNGase F - New England Biolabs France\" \/>\n<meta property=\"og:description\" content=\"[vc_row][vc_column][vc_column_text] PNGase F is the most effective enzymatic method for removing almost all N-linked oligosaccharides from glycoproteins. PNGase F digestion deaminates the asparagine residue to aspartic acid, and leaves the oligosaccharide intact, keeping it suitable for further analysis. Rapid PNGase F is an improved reagent that allows the complete and rapid deglycosylation of antibodies and... 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PNGase F digestion deaminates the asparagine residue to aspartic acid, and leaves the oligosaccharide intact, keeping it suitable for further analysis. Rapid PNGase F is an improved reagent that allows the complete and rapid deglycosylation of antibodies and... 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