{"id":2267,"date":"2015-04-29T08:25:33","date_gmt":"2015-04-29T06:25:33","guid":{"rendered":"http:\/\/www.neb-online.fr\/?page_id=2267"},"modified":"2022-11-14T12:43:51","modified_gmt":"2022-11-14T11:43:51","slug":"epimark-kits","status":"publish","type":"page","link":"https:\/\/www.neb-online.fr\/en\/epigenetics\/epimark-kits\/","title":{"rendered":"EpiMark Kits"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row][vc_column width=&#8221;1\/1&#8243; css=&#8221;.vc_custom_1429652502561{margin-bottom: 25px !important;}&#8221;][vc_column_text]<\/p>\n<h2>EpiMark Methylated DNA Enrichment Kit<\/h2>\n<p>[\/vc_column_text][vc_column_text]The\u00a0<a href=\"https:\/\/shop.neb-online.fr\/en\/art\/E2600S\" target=\"_blank\" rel=\"noopener noreferrer\">EpiMark\u00ae Methylated DNA Enrichment Kit<\/a>\u00a0enables the enrichment of double-stranded CpG methylated DNA based on CpG methylation density. It utilizes the methyl-CpG binding domain of human MBD2a protein as a capture agent. The protein is fused to the Fc tail of human IgG1 (MBD2a-Fc), which is coupled to Protein A Magnetic Beads (MBD2a-Fc\/Protein A Bead). This stable complex will selectively bind double-stranded methylated CpG containing DNA. The high binding affinity of the beads coupled with optimized reagents increases sensitivity and accuracy. This kit contains all the individual components necessary to achieve enrichment in less than two hours using a four step process.[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]<strong>Workflow:<\/strong>[\/vc_column_text][vc_single_image image=&#8221;1729&#8243; border_color=&#8221;grey&#8221; img_link_large=&#8221;yes&#8221; img_link_target=&#8221;_blank&#8221; img_size=&#8221;full&#8221;][\/vc_column][vc_column width=&#8221;1\/2&#8243;][vc_column_text]<strong>Advantages:<\/strong><\/p>\n<ul>\n<li>Increased sensitivity<\/li>\n<li>Easy-to-use protocol yields enriched methylated DNA in less than 2 hours<\/li>\n<li>Amenable to downstream applications, including next generation sequencing<\/li>\n<li>Suitable for low levels of input DNA<\/li>\n<\/ul>\n<p>[\/vc_column_text][vc_column_text]<strong>Use directly in downstream analysis:<\/strong><\/p>\n<ul>\n<li><a title=\"PCR and DNA Amplification\" href=\"https:\/\/www.neb-online.fr\/pcr-and-dna-amplification\/\">Endpoint PCR<\/a><\/li>\n<li>Real-time PCR<\/li>\n<li>Bisulfite conversion<\/li>\n<li><a title=\"Cloning \/ Synthetic Biology\" href=\"http:\/\/www.neb-online.fr\/en\/neb-encloning-synthetic-biology\/\">Cloning<\/a>\u00a0and sequencing<\/li>\n<li>Direct sequencing<\/li>\n<li><a title=\"Next Generation Sequencing\" href=\"http:\/\/www.neb-online.fr\/en\/neb-ennext-generation-sequencing\/\">Library preparation for high throughput sequencing<\/a><\/li>\n<li>Labelling for DNA microarray analysis<\/li>\n<li><a title=\"Restriction Enzymes for Epigenetics\" href=\"http:\/\/www.neb-online.fr\/en\/neb-enepigenetics\/restriction-enzymes-for-epigenetics\/\">Methylation-sensitive restriction enzyme-based assays<\/a><\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/1&#8243;][vc_separator color=&#8221;grey&#8221; align=&#8221;align_center&#8221;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/2&#8243;][vc_single_image image=&#8221;1730&#8243; border_color=&#8221;grey&#8221; img_link_large=&#8221;yes&#8221; img_link_target=&#8221;_blank&#8221; img_size=&#8221;full&#8221;][vc_column_text]<span class=\"bildunterschrift\"><em>Comparison of the EpiMark Methylated DNA Enrichment Kit to other commercially available DNA enrichment kits shows enhanced sensitivity<\/em><strong><em><br \/>\n<\/em><\/strong><\/span>[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/2&#8243;][vc_single_image image=&#8221;1731&#8243; border_color=&#8221;grey&#8221; img_link_large=&#8221;yes&#8221; img_link_target=&#8221;_blank&#8221; img_size=&#8221;full&#8221;][vc_column_text]<span class=\"bildunterschrift\"><em>The Epimark Methylated DNA Enrichment Kit shows excellent signal-to-noise, even at very low levels of input DNA<\/em><\/span>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/1&#8243;][vc_separator color=&#8221;grey&#8221; align=&#8221;align_center&#8221;][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<\/p>\n<h2>Available product<\/h2>\n<table style=\"width: 760px;\" border=\"0\" cellpadding=\"5\">\n<tbody>\n<tr style=\"background-color: #f3f3f3; font-size: 16px;\">\n<th style=\"border-bottom: solid #eb7e2d;\" align=\"left\" width=\"400\" height=\"40px\">PRODUCT<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"88\">REF.<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"100\">SIZE<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" colspan=\"1\">PRICE<\/th>\n<\/tr>\n<tr style=\"background-color: #ffffff;\">\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"1\">EpiMark\u00ae Methylated DNA Enrichment Kit<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E2600 S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">25 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/neb-online.fr\/en\/art\/E2600S\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator color=&#8221;black&#8221;][vc_column_text]<\/p>\n<h2>EpiMark 5-mC and 5-hmC Analysis Kit<\/h2>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]<strong>Breakthrough in 5-hmC Quantitation for Epigenetics<\/strong><\/p>\n<p>New England Biolabs is pleased to introduce the\u00a0<a href=\"https:\/\/neb-online.fr\/en\/art\/E2600S\" target=\"_blank\" rel=\"noopener noreferrer\">EpiMark\u00ae 5-hmC and 5-mC Analysis Kit<\/a>, a simple and robust method for the identification and quantitation of 5-hydroxymethylcytosine (5-hmC) and 5-methylcytosine (5-mC) within a specific DNA locus. This enzymatic approach utilizes the differential methylation sensitivity of the isoschizomers\u00a0<a href=\"https:\/\/neb-online.fr\/en\/art\/R0106@\" target=\"_blank\" rel=\"noopener noreferrer\">MspI<\/a>\u00a0and\u00a0<a href=\"https:\/\/neb-online.fr\/en\/art\/R0171@\" target=\"_blank\" rel=\"noopener noreferrer\">HpaII<\/a>\u00a0in a simple 3-step protocol (described below). The EpiMark Kit is the first commercially available PCR-based assay to reproducibly identify and quantitate the presence of 5-hmC and 5-mC, and expands the potential for new biomarker discovery.[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/2&#8243;][vc_column_text]<strong>Advantages:<\/strong><\/p>\n<ul>\n<li>Reproducible quantitation of 5-hmC and 5-mC<\/li>\n<li>Easy-to-use protocols<\/li>\n<li>Compatible with existing techniques (PCR)<\/li>\n<li>Amenable to high throughpu<\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/1&#8243;][vc_separator color=&#8221;grey&#8221; align=&#8221;align_center&#8221;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/1&#8243;][vc_column_text]<em><strong>Overview of 5-hmC and 5-mC identification using the EpiMark 5-hmC and 5-mC Analysis Kit<\/strong><\/em><strong>\u00a0<\/strong>Click to enlarge[\/vc_column_text][vc_single_image image=&#8221;1240&#8243; border_color=&#8221;grey&#8221; img_link_large=&#8221;yes&#8221; img_link_target=&#8221;_blank&#8221; img_size=&#8221;full&#8221;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/1&#8243;][vc_separator color=&#8221;grey&#8221; align=&#8221;align_center&#8221;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/1&#8243;][vc_single_image image=&#8221;1239&#8243; border_color=&#8221;grey&#8221; img_link_large=&#8221;&#8221; img_link_target=&#8221;_self&#8221; img_size=&#8221;full&#8221; css=&#8221;.vc_custom_1429217790551{margin-bottom: 6px !important;}&#8221;][vc_column_text]<span class=\"bildunterschrift\"><em><strong>Analysis of the different methylation states in Balb\/C mouse tissue samples using the EpiMark 5-hmC and 5-mC Analysis Kit.<\/strong><\/em><\/span><\/p>\n<p><span class=\"bildunterschrift\"><em>A) Endpoint PCR of the 6 different reactions needed for methylation analysis. The boxed lanes indicate the presence of 5-hmC.<br \/>\nB) Real time PCR data was used to determine amounts of 5-hmC and 5-mC present. The results demonstrate a variation in 5-hmC levels in the tissue sources indicated.<\/em><\/span>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/1&#8243;][vc_separator color=&#8221;grey&#8221; align=&#8221;align_center&#8221;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/1&#8243;][vc_column_text]<\/p>\n<h2>Available products<\/h2>\n<table style=\"width: 760px;\" border=\"0\" cellpadding=\"5\">\n<tbody>\n<tr style=\"background-color: #f3f3f3; font-size: 16px;\">\n<th style=\"border-bottom: solid #eb7e2d;\" align=\"left\" width=\"400\" height=\"40px\">PRODUCT<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"88\">REF.<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"100\">SIZE<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" colspan=\"1\">PRICE<\/th>\n<\/tr>\n<tr style=\"background-color: #ffffff;\">\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"1\">EpiMark\u00ae 5-hmC and 5-mC Analysis Kit<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E3317 S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">20 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/neb-online.fr\/en\/art\/E3317S\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr style=\"background-color: #ffffff;\">\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"2\">T4 Phage \u00df-glucosyltransferase<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0357 S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">500 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/neb-online.fr\/en\/art\/M0357@\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr style=\"background-color: #ffffff;\">\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\" rowspan=\"2\">M0357 L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">2 500 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\"><a href=\"https:\/\/neb-online.fr\/en\/art\/M0357@\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator color=&#8221;black&#8221; align=&#8221;align_center&#8221;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;2\/3&#8243;][vc_column_text]<\/p>\n<h2>EpiMark Bisulfite Conversion Kit<\/h2>\n<p>Sodium bisulfite conversion is the current &#8220;gold standard&#8221; in methylation analysis. This technique can reveal the methylation status of every cytosine residue, and it is amenable to\u00a0<a title=\"Next Generation Sequencing\" href=\"http:\/\/www.neb-online.fr\/en\/neb-ennext-generation-sequencing\/\">massively parallel sequencing methods<\/a>.<\/p>\n<p>Bisulfite conversion, involves the conversion of unmodified cytosines to uracil, leaving the modified bases (5-mC and 5-hmC). The\u00a0<a href=\"https:\/\/neb-online.fr\/en\/art\/E3318S\" target=\"_blank\" rel=\"noopener noreferrer\">EpiMark\u00ae Bisulfite Conversion Kit<\/a>\u00a0is designed for the detection of methylated cytosine, using a series of alternating cycles of thermal denaturation, followed by incubation with sodium bisulfite. This kit includes all the reagents necessary for complete bisulfite conversion, including spin columns. Amplification of bisulfite-treated samples can then be performed using\u00a0<a href=\"https:\/\/neb-online.fr\/en\/art\/M0490@\" target=\"_blank\" rel=\"noopener noreferrer\">EpiMark\u00ae Hot Start Taq DNA Polymerase<\/a>.[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/3&#8243; offset=&#8221;vc_col-lg-4 vc_col-md-4 vc_col-xs-6&#8243;][vc_single_image image=&#8221;1458&#8243; border_color=&#8221;grey&#8221; img_link_large=&#8221;&#8221; img_link_target=&#8221;_self&#8221; img_size=&#8221;full&#8221; css=&#8221;.vc_custom_1429653215352{margin-bottom: 5px !important;}&#8221;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/1&#8243;][vc_column_text]<strong>Advantages<\/strong><\/p>\n<ul>\n<li>Complete conversion of unmodified cytosines to uracil<\/li>\n<li>Easy-to-use protocol ensures reliable and consistent results<\/li>\n<li>All reagents, including purification columns, are provided<\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/1&#8243;][vc_column_text]<b>Overview of bisulfite conversion<\/b>[\/vc_column_text][vc_single_image image=&#8221;1743&#8243; border_color=&#8221;grey&#8221; img_link_large=&#8221;&#8221; img_link_target=&#8221;_self&#8221; img_size=&#8221;full&#8221;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/1&#8243;][vc_separator color=&#8221;grey&#8221; align=&#8221;align_center&#8221;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/2&#8243;][vc_single_image image=&#8221;1459&#8243; border_color=&#8221;grey&#8221; img_link_large=&#8221;&#8221; img_link_target=&#8221;_self&#8221; img_size=&#8221;full&#8221; css=&#8221;.vc_custom_1429652960346{margin-bottom: 5px !important;}&#8221;][\/vc_column][vc_column width=&#8221;1\/2&#8243;][vc_column_text]<span class=\"bildunterschrift\"><strong><em>EpiMark Kit enables complete DNA conversion<\/em><\/strong><\/span><\/p>\n<p><span class=\"bildunterschrift\"><em>1 \u00b5g of genomic DNA was bisulfite-treated using the Epimark Bisulfite Conversion Kit, and 2 \u00b5l of eluted DNA from 40 \u00b5l of elutant was analyzed by end-point PCR using EpiMark Hot Start Taq. Amplification with primer pairs for bisulfite converted DNA (lanes 1, 3, and 5), or with primer pairs for unconverted DNA (lanes 2, 4, and 6) were performed; lanes 2, 4, and 6 show no amplification product, indicating complete conversion.<\/em><\/span>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/1&#8243;][vc_separator color=&#8221;black&#8221; align=&#8221;align_center&#8221;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/1&#8243;][vc_column_text]<\/p>\n<h2>EpiMark Hot Start DNA Polymerase<\/h2>\n<p><strong>EpiMark\u00ae Hot Start Taq DNA \u2028Polymerase &#8211; an excellent choice for use on bisulfite-treated DNA<\/strong><br \/>\n<a href=\"https:\/\/neb-online.fr\/en\/art\/M0490@\" target=\"_blank\" rel=\"noopener noreferrer\">EpiMark Hot Start Taq DNA Polymerase<\/a>\u00a0is a mixture of Taq DNA Polymerase and a temperature sensitive, aptamer-based inhibitor. This inhibitor binds reversibly to the enzyme, inhibiting polymerase activity below 45\u00b0C, but releases the enzyme during normal PCR cycling conditions. This permits PCR reactions to be assembled at room temperature and eliminates an activation step. This aptamer-based hot start activity combined with the supplied reaction buffer, that has been optimized for amplification of converted DNA, makes EpiMark Hot Start Taq an excellent choice for use on bisulfite-treated DNA.[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/1&#8243;][vc_column_text]<\/p>\n<h2>Available products<\/h2>\n<table style=\"width: 760px;\" border=\"0\" cellpadding=\"5\">\n<tbody>\n<tr style=\"background-color: #f3f3f3; font-size: 16px;\">\n<th style=\"border-bottom: solid #eb7e2d;\" align=\"left\" width=\"400\" height=\"40px\">PRODUCT<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"88\">REF.<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"100\">SIZE<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" colspan=\"1\">PRICE<\/th>\n<\/tr>\n<tr style=\"background-color: #ffffff;\">\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"1\">EpiMark\u00ae Bisulfite Conversion Kit<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E3318 S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">48 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/neb-online.fr\/en\/art\/E3318S\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr style=\"background-color: #ffffff;\">\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"2\">EpiMark\u00ae Hot Start Taq DNA Polymerase<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0490 S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/neb-online.fr\/en\/art\/M0490@\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr style=\"background-color: #ffffff;\">\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\" rowspan=\"2\">M0490 L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">500 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\"><a href=\"https:\/\/neb-online.fr\/en\/art\/M0490@\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/1&#8243;][vc_separator color=&#8221;black&#8221; align=&#8221;align_center&#8221;][vc_column_text]<\/p>\n<h2>EpiMark Nucleosome Assembly Kit<\/h2>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]In eukaryotes, chromatin is organized into nucleosome core particles (NCPs) that consist of approximately 147 bp of DNA and an octamer complex made up of two molecules of each histone (H2A, H2B, H3 and H4). The linker histone, H1, can further condense chromatin by binding to linker DNA between the nucleosome core particles. For histone modification studies, New England Biolabs offers a selection of unmodified, recombinant human histones that function as substrates and can assemble into octamers. Octamer formation can be further simplified using the\u00a0<a href=\"https:\/\/neb-online.fr\/en\/art\/E5350S\" target=\"_blank\" rel=\"noopener noreferrer\">EpiMark Nucleosome Assembly Kit<\/a>\u00a0which contains preformed Histone H2A\/H2B Dimer and Histone H3.1\/H4 Tetramer.[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/2&#8243;][vc_column_text]<strong>Applications:<\/strong><\/p>\n<ul>\n<li>Purification and characterization of enzymes that modify histone proteins<\/li>\n<li>Octamer modification studies<\/li>\n<li>Carrier chromatin immunoprecipitation (CChIP)<\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/1&#8243;][vc_column_text]The EpiMark Nucleosome Assembly Kit contains all the components necessary for recombinant human nucleosome formation with user-supplied or the provided control DNA. These recombinant nucleosomes may serve as better substrates for enzymes (e.g. acetylases, methylases, sumoylases) or other chromatin interacting proteins that are unable to bind and\/or modify individual histones or DNA alone. Additionally, the\u00a0<a href=\"https:\/\/neb-online.fr\/en\/art\/M2508S\" target=\"_blank\" rel=\"noopener noreferrer\">Histone H2A\/H2B Dimer<\/a>\u00a0and the\u00a0<a href=\"https:\/\/neb-online.fr\/en\/art\/M2509S\" target=\"_blank\" rel=\"noopener noreferrer\">Histone H3.1\/H4 Tetramer<\/a>\u00a0may themselves contain the required fold or structure to serve as more favorable substrates than the individual histones.[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/2&#8243;][vc_single_image image=&#8221;1749&#8243; border_color=&#8221;grey&#8221; img_link_large=&#8221;&#8221; img_link_target=&#8221;_self&#8221; img_size=&#8221;full&#8221; alignment=&#8221;center&#8221;][\/vc_column][vc_column width=&#8221;1\/2&#8243;][vc_column_text]<span class=\"bildunterschrift\"><em><strong>Gel shift assay to visualize nucleosome assembly<\/strong><br \/>\nSamples from nucleosome assembly reactions were run on 6% polyacrylamide gel in 0.5X TBE.<br \/>\nLane 1: Low Molecular Weight DNA Ladder (NEB #N3233)<br \/>\nLane 2: Nucleosome Control DNA<br \/>\nLane 3: 0.5:1 ratio of Octamer* to DNA<br \/>\nLane 4: 1:1 ratio of Octamer* to DNA<br \/>\n*Octamer = 2:1 mix of Histone H2A\/H2B Dimer and Histone H3.1\/H4 Tetramer.<\/em><\/span>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/1&#8243;][vc_column_text css=&#8221;.vc_custom_1430297342253{margin-bottom: 5px !important;}&#8221;]<\/p>\n<h2>Available products<\/h2>\n<table style=\"width: 760px;\" border=\"0\" cellpadding=\"5\">\n<tbody>\n<tr style=\"background-color: #f3f3f3; font-size: 16px;\">\n<th style=\"border-bottom: solid #eb7e2d;\" align=\"left\" width=\"400\" height=\"40px\">PRODUCT<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"88\">REF.<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"100\">SIZE<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" colspan=\"1\">PRICE<\/th>\n<\/tr>\n<tr style=\"background-color: #ffffff;\">\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"1\">EpiMark\u00ae Nucleosome Assembly Kit<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E5350 S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">20 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/neb-online.fr\/en\/art\/E5350S\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr style=\"background-color: #ffffff;\">\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"1\">H1<span style=\"font-size: 10pt;\"><sup>0<\/sup><\/span> Human, Recombinant<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M2501 S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 \u00b5g<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/neb-online.fr\/en\/art\/M2501S\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr style=\"background-color: #ffffff;\">\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"1\">H2A Human, Recombinant<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M2502 S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 \u00b5g<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/neb-online.fr\/en\/art\/M2502S\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr style=\"background-color: #ffffff;\">\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"1\">H2B Human, Recombinant<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M2505 S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 \u00b5g<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/neb-online.fr\/en\/art\/M2505S\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr style=\"background-color: #ffffff;\">\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"1\">H3.1 Human, Recombinant<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M2503 S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 \u00b5g<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/neb-online.fr\/en\/art\/M2503S\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr style=\"background-color: #ffffff;\">\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"1\">H3.2 Human, Recombinant<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M2506 S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 \u00b5g<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/neb-online.fr\/en\/art\/M2506S\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr style=\"background-color: #ffffff;\">\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"1\">H3.3 Human, Recombinant<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M2507 S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 \u00b5g<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/neb-online.fr\/en\/art\/M2507S\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr style=\"background-color: #ffffff;\">\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"1\">H4 Human, Recombinant<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M2504 S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 \u00b5g<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/neb-online.fr\/en\/art\/M2504S\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr style=\"background-color: #ffffff;\">\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"1\">Histone H3.1 \/ H4 Tetramer Human, Recombinant<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M2509 S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">1 nmol<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/neb-online.fr\/en\/art\/M2509S\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr style=\"background-color: #ffffff;\">\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"1\">Histone H2A\/H2B Dinner Human, Recombinant<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M2508 S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">2 nmol<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/neb-online.fr\/en\/art\/M2508S\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr style=\"background-color: #ffffff;\">\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"1\">Nucleosome control DNA<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">N1202 S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">0,2 nmol<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/neb-online.fr\/en\/art\/N1202S\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row][vc_column width=&#8221;1\/1&#8243; css=&#8221;.vc_custom_1429652502561{margin-bottom: 25px !important;}&#8221;][vc_column_text] EpiMark Methylated DNA Enrichment Kit [\/vc_column_text][vc_column_text]The\u00a0EpiMark\u00ae Methylated DNA Enrichment Kit\u00a0enables the enrichment of double-stranded CpG methylated DNA based on CpG methylation density. It utilizes the methyl-CpG binding domain of human MBD2a protein as a capture agent. The protein is fused to the Fc tail of human IgG1 (MBD2a-Fc), which is&#8230;  <a class=\"excerpt-read-more\" href=\"https:\/\/www.neb-online.fr\/en\/epigenetics\/epimark-kits\/\" title=\"Read EpiMark Kits\">Read more &raquo;<\/a><\/p>\n","protected":false},"author":15,"featured_media":1726,"parent":2264,"menu_order":0,"comment_status":"open","ping_status":"closed","template":"page-sidebar-epigenetics.php","meta":{"footnotes":""},"class_list":["post-2267","page","type-page","status-publish","has-post-thumbnail","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.0 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>EpiMark Kits - New England Biolabs France<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.neb-online.fr\/en\/epigenetics\/epimark-kits\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"EpiMark Kits - New England Biolabs France\" \/>\n<meta property=\"og:description\" content=\"[vc_row][vc_column width=&#8221;1\/1&#8243; css=&#8221;.vc_custom_1429652502561{margin-bottom: 25px !important;}&#8221;][vc_column_text] EpiMark Methylated DNA Enrichment Kit [\/vc_column_text][vc_column_text]The\u00a0EpiMark\u00ae Methylated DNA Enrichment Kit\u00a0enables the enrichment of double-stranded CpG methylated DNA based on CpG methylation density. It utilizes the methyl-CpG binding domain of human MBD2a protein as a capture agent. The protein is fused to the Fc tail of human IgG1 (MBD2a-Fc), which is... 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