{"id":2298,"date":"2015-04-29T11:24:31","date_gmt":"2015-04-29T09:24:31","guid":{"rendered":"http:\/\/www.neb-online.fr\/?page_id=2298"},"modified":"2020-04-27T18:57:07","modified_gmt":"2020-04-27T16:57:07","slug":"cellular-analysis-snap-tag","status":"publish","type":"page","link":"https:\/\/www.neb-online.fr\/en\/cellular-analysis-snap-tag\/","title":{"rendered":"Cellular Analysis (SNAP-Tag)"},"content":{"rendered":"

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SNAP-tag Technology<\/h2>\n
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SNAP- and CLIP-tag protein labeling systems enable the specific, covalent attachment of virtually any molecule to a protein of interest. There are two steps to using this system: cloning and expression of the protein of interest as a SNAP-tag\u00ae fusion, and labeling of the fusion with the SNAP-tag substrate of choice. The SNAP-tag is a small protein based on human O6<\/sup>-alkylguanine-DNA-alkyltransferase (hAGT), a DNA repair protein.<\/p>\n

SNAP-tag substrates are dyes, fluorophores, biotin, or beads conjugated to guanine or chloropyrimidine leaving groups via a benzyl linker. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag. CLIP-tag\u2122 is a modified version of SNAP-tag, engineered to react with benzylcytosine rather than benzylguanine derivatives. When used in conjunction with SNAP-tag, CLIP-tag enables the orthogonal and complementary labeling of two proteins simultaneously in the same cells.<\/p>\n<\/div>\n<\/article>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n

[\/vc_column_text][\/vc_column][vc_column width=”1\/2″][vc_single_image image=”1338″ img_size=”full” img_link_large=”yes”][vc_column_text css=”.vc_custom_1431961803887{margin-bottom: 5px !important;}”]The SNAP- or CLIP-tag is fused to the protein of interest. Labeling occurs through covalent attachment to the tag, releasing either a guanine or a cytosine moiety.<\/em><\/span>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text][\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator color=”custom” accent_color=”#919191″][\/vc_column][\/vc_row][vc_row][vc_column width=”1\/2″][vc_column_text css=”.vc_custom_1429518974615{margin-bottom: 5px !important;}”]<\/p>\n

Workflow<\/h3>\n

[\/vc_column_text][vc_single_image image=”1340″ img_size=”full” img_link_large=”yes” css=”.vc_custom_1433319432503{margin-bottom: 5px !important;}”][vc_column_text]Clone and express your protein of interest fused to the SNAP-tag once, then use with a variety of\u00a0substrates for subsequent analysis.<\/span>[\/vc_column_text][\/vc_column][vc_column width=”1\/2″ css=”.vc_custom_1429285339891{margin-top: 40px !important;}”][vc_single_image image=”1342″ img_size=”full” onclick=”custom_link” img_link_target=”_blank” link=”https:\/\/www.neb.com\/tools-and-resources\/video-library?device=modal&videoid={9FDA0B32-2522-4E53-A98D-67184F5A5652}” css=”.vc_custom_1434012978814{margin-bottom: 5px !important;}”][vc_column_text]Fluorescent Labeling of COS-7 Expressing SNAP-tag\u00ae<\/sup>\u00a0Fusion Proteins for Live Cell Imaging<\/strong>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator color=”custom” accent_color=”#919191″][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=”.vc_custom_1430308058322{margin-bottom: 30px !important;}”]<\/p>\n

Applications<\/h3>\n

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