{"id":4960,"date":"2015-06-15T08:39:14","date_gmt":"2015-06-15T08:39:14","guid":{"rendered":"http:\/\/www.neb-online.fr\/?page_id=4960"},"modified":"2020-04-16T22:38:54","modified_gmt":"2020-04-16T20:38:54","slug":"nebnext-library-quant-kit-for-illumina","status":"publish","type":"page","link":"https:\/\/www.neb-online.fr\/en\/next-generation-sequencing\/nebnext-library-quant-kit-for-illumina\/","title":{"rendered":"NEBNext Library Quant Kit for Illumina"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]Accurate quantitation of next-generation sequencing libraries is essential for maximizing data output and quality from each sequencing run.<\/p>\n<p>Furthermore, for Illumina sequencing, accurate quantitation of libraries is critical to achieve optimal cluster densities, a requirement for optimal sequence output.<\/p>\n<p>qPCR is considered to be the most accurate and effective method of library quantitation, providing considerably higher consistency and reproducibility of quantitation.<\/p>\n<p>Amplification-based methods quantitate only those molecules that contain both adaptor sequences, thereby providing a more accurate estimate of the concentration of the library molecules that can be sequenced.<\/p>\n<p>The NEBNext Library Quant Kit delivers significant improvements to qPCR-based library quantitation for next gen sequencing.[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/2&#8243;]<div class=\"vbox\">\n\t\t\t\t<span class=\"vhead\">Advantages:<\/span>\n\t\t\t\t\t<ul class=\"vlist\">\n\t\t\t\t\t\t<li><span>Be confident in your quant values, as our kit provides more accurate and reproducible results than other methods and kits.<br \/><\/span><\/li><li><span>Get up and running quickly with our easy-to-use kit, containing Library Dilution Buffer, optimized master mix, 4 standards and ROX dye.<br \/><\/span><\/li><li><span>Simplify your reaction setup with fewer pipetting steps and a single extension time for all libraries.<br \/><\/span><\/li><li><span>Quantitate more libraries per kit, as only 4 standards are required.<br \/><\/span><\/li><li><span>Use with all your libraries, regardless of insert size, GC content and preparation method.<br \/><\/span><\/li><li><span>Save money with our value pricing.<\/span><\/li>\n\t\t\t\t\t<\/ul>\n\t\t\t<\/div>[vc_row_inner][vc_column_inner][vc_column_text][\/vc_column_text][vc_column_text]Want a special offer?<br \/>\nPlease contact us.[\/vc_column_text]<a class=\"btn btn-standard form-btn\" target=\"_blank\" href=\"http:\/\/www.neb-online.fr\/en\/contact\/\" style=\"margin-right:auto;\">Click Here<\/a>[\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][\/vc_column][\/vc_row][vc_row][vc_column][vc_single_image image=&#8221;9188&#8243; img_size=&#8221;full&#8221; alignment=&#8221;center&#8221;][vc_column_text]<\/p>\n<p style=\"text-align: center;\">Feature Article:<\/p>\n<p style=\"text-align: center;\">\u201eThe Quantitation Question: How does accurate<br \/>\nlibrary quantitation influence sequencing?\u201c<\/p>\n<p>[\/vc_column_text]<a class=\"btn btn-standard form-btn\"  href=\"http:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/10\/FEATURE_ARTICLE_NEBNext_QuantKit.pdf\" style=\"margin-left:auto;margin-right:auto;\">Download PDF<\/a>[\/vc_column][\/vc_row][vc_row][vc_column][vc_separator color=&#8221;black&#8221;][vc_column_text]<br \/>\nWith NEBNext, optimal cluster density is achieved from quantitated libraries with a broad range of library size and GC content.<\/p>\n<p>[\/vc_column_text][vc_single_image image=&#8221;4967&#8243; img_size=&#8221;full&#8221; alignment=&#8221;center&#8221; img_link_large=&#8221;yes&#8221;][vc_column_text]Libraries of 310\u2013963 bp from the indicated sources were quantitated using the NEBNext Library Quant Kit, then diluted to 8 pM and loaded onto a MiSeq\u00ae (v2 chemistry; MCS v2.4.1.3). Library concentrations ranged from 7\u2013120 nM, and resulting raw cluster density for all libraries was 965\u20131300 k\/mm2 (ave. =1199). Optimal cluster density was achieved using concentrations determined by the NEBNext Library Quant Kit for all library sizes.[\/vc_column_text][vc_separator][vc_single_image image=&#8221;4978&#8243; img_size=&#8221;full&#8221; alignment=&#8221;center&#8221; img_link_large=&#8221;yes&#8221;][vc_column_text]Greater reproducibility of library quantitation with the NEBNext Library Quant Kit<\/p>\n<p>Three 340\u2013400 bp libraries were quantitated by 4 different users 2\u20134 times using either the NEBNext or KapaTM Library Quantification Kit (Universal). A notable improvement in quantitation consistency was observed for concentrations determined by the NEBNext Kit (orange) versus those from the Kapa kit (gray). [\/vc_column_text][vc_separator][vc_single_image image=&#8221;4979&#8243; img_size=&#8221;full&#8221; alignment=&#8221;center&#8221; img_link_large=&#8221;yes&#8221;][vc_column_text]The NEBNext Library Quant Kit values enable optimal cluster densities<\/p>\n<p>Seven different libraries were quantitated using either the NEBNext Library Quant Kit (orange) or the Kapa Library Quantification Kit (Universal) (gray). Undiluted library concentrations ranged from 2\u2013200 nM. Libraries were diluted to 8 pM and loaded onto a MiSeq\u00ae instrument (v2 chemistry; MCS v2.4.1.3). Libraries quantitated with the NEBNext kit resulted in a raw cluster density average of 1160 k\/mm2, directly in the optimal range of 900\u20131300 k\/mm2. In contrast, libraries loaded based on the Kapa quantitation averaged only 660 k\/mm2. [\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][vc_column_text]<\/p>\n<h2>Available products<\/h2>\n<table style=\"width: 760px;\" border=\"0\" cellpadding=\"5\">\n<tbody>\n<tr style=\"background-color: #f3b281;\">\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"396\" height=\"40px\">PRODUCT<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"80\">Prod.Nr.:<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"132\">SIZE<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" colspan=\"1\" width=\"152\">PRICE<\/th>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">NEBNext Library Quant Kit for Illumina<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E7630 S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/e7630s\"><img decoding=\"async\" class=\"size-full wp-image-4375 aligncenter\" src=\"http:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">NEBNext Library Quant Kit for Illumina<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E7630 L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">500 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/=e7630l\"><img decoding=\"async\" class=\"size-full wp-image-4375 aligncenter\" src=\"http:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]Accurate quantitation of next-generation sequencing libraries is essential for maximizing data output and quality from each sequencing run. Furthermore, for Illumina sequencing, accurate quantitation of libraries is critical to achieve optimal cluster densities, a requirement for optimal sequence output. qPCR is considered to be the most accurate and effective method of library quantitation, providing&#8230;  <a class=\"excerpt-read-more\" href=\"https:\/\/www.neb-online.fr\/en\/next-generation-sequencing\/nebnext-library-quant-kit-for-illumina\/\" title=\"Read NEBNext Library Quant Kit for Illumina\">Read more &raquo;<\/a><\/p>\n","protected":false},"author":15,"featured_media":5021,"parent":2170,"menu_order":0,"comment_status":"open","ping_status":"open","template":"page-sidebar-next-generation-sequencing.php","meta":{"footnotes":""},"class_list":["post-4960","page","type-page","status-publish","has-post-thumbnail","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.0 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>NEBNext Library Quant Kit for Illumina - New England Biolabs France<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.neb-online.fr\/en\/next-generation-sequencing\/nebnext-library-quant-kit-for-illumina\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"NEBNext Library Quant Kit for Illumina - New England Biolabs France\" \/>\n<meta property=\"og:description\" content=\"[vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]Accurate quantitation of next-generation sequencing libraries is essential for maximizing data output and quality from each sequencing run. Furthermore, for Illumina sequencing, accurate quantitation of libraries is critical to achieve optimal cluster densities, a requirement for optimal sequence output. qPCR is considered to be the most accurate and effective method of library quantitation, providing... Read more &raquo;\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.neb-online.fr\/en\/next-generation-sequencing\/nebnext-library-quant-kit-for-illumina\/\" \/>\n<meta property=\"og:site_name\" content=\"New England Biolabs France\" \/>\n<meta property=\"article:modified_time\" content=\"2020-04-16T20:38:54+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/06\/nebnext_quantkit_760px.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"760\" \/>\n\t<meta property=\"og:image:height\" content=\"200\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"3 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.neb-online.fr\/en\/next-generation-sequencing\/nebnext-library-quant-kit-for-illumina\/\",\"url\":\"https:\/\/www.neb-online.fr\/en\/next-generation-sequencing\/nebnext-library-quant-kit-for-illumina\/\",\"name\":\"NEBNext Library Quant Kit for Illumina - New England Biolabs France\",\"isPartOf\":{\"@id\":\"https:\/\/www.neb-online.fr\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\/\/www.neb-online.fr\/en\/next-generation-sequencing\/nebnext-library-quant-kit-for-illumina\/#primaryimage\"},\"image\":{\"@id\":\"https:\/\/www.neb-online.fr\/en\/next-generation-sequencing\/nebnext-library-quant-kit-for-illumina\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/06\/nebnext_quantkit_760px.jpg\",\"datePublished\":\"2015-06-15T08:39:14+00:00\",\"dateModified\":\"2020-04-16T20:38:54+00:00\",\"breadcrumb\":{\"@id\":\"https:\/\/www.neb-online.fr\/en\/next-generation-sequencing\/nebnext-library-quant-kit-for-illumina\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/www.neb-online.fr\/en\/next-generation-sequencing\/nebnext-library-quant-kit-for-illumina\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/www.neb-online.fr\/en\/next-generation-sequencing\/nebnext-library-quant-kit-for-illumina\/#primaryimage\",\"url\":\"https:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/06\/nebnext_quantkit_760px.jpg\",\"contentUrl\":\"https:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/06\/nebnext_quantkit_760px.jpg\",\"width\":760,\"height\":200,\"caption\":\"NEBNext Quant Kit\"},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/www.neb-online.fr\/en\/next-generation-sequencing\/nebnext-library-quant-kit-for-illumina\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Next Generation Sequencing\",\"item\":\"https:\/\/www.neb-online.fr\/en\/next-generation-sequencing\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"NEBNext Library Quant Kit for Illumina\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/www.neb-online.fr\/#website\",\"url\":\"https:\/\/www.neb-online.fr\/\",\"name\":\"New England Biolabs France\",\"description\":\"be INSPIRED, drive DISCOVERY, stay GENUINE\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/www.neb-online.fr\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"NEBNext Library Quant Kit for Illumina - New England Biolabs France","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.neb-online.fr\/en\/next-generation-sequencing\/nebnext-library-quant-kit-for-illumina\/","og_locale":"en_US","og_type":"article","og_title":"NEBNext Library Quant Kit for Illumina - New England Biolabs France","og_description":"[vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]Accurate quantitation of next-generation sequencing libraries is essential for maximizing data output and quality from each sequencing run. 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