{"id":5399,"date":"2015-07-22T11:59:38","date_gmt":"2015-07-22T11:59:38","guid":{"rendered":"http:\/\/www.neb-online.fr\/en\/?page_id=5399"},"modified":"2020-04-16T23:13:06","modified_gmt":"2020-04-16T21:13:06","slug":"nebnext-ffpe-dna-repair-mix","status":"publish","type":"page","link":"https:\/\/www.neb-online.fr\/en\/next-generation-sequencing\/nebnext-ffpe-dna-repair-mix\/","title":{"rendered":"NEBNext FFPE DNA Repair Mix"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p><span data-mce-type=\"bookmark\" style=\"display: inline-block; width: 0px; overflow: hidden; line-height: 0;\" class=\"mce_SELRES_start\">\ufeff<\/span>[vc_row][vc_column width=&#8221;2\/3&#8243;][vc_column_text]Archiving of clinical materials as Formalin-Fixed, Paraffin-Embedded (FFPE) samples is a common practice. However, the methods used for fixation and storage significantly damage and compromise the quality of nucleic acids from these samples. As a result, it can be challenging to obtain useful information, including high-quality sequence data, especially when sample amounts are limited. The NEBNext FFPE DNA Repair Mix is a cocktail of enzymes formulated to repair DNA, and specifically optimized and validated for repair of FFPE DNA samples.[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/3&#8243;]<div class=\"vbox\">\n\t\t\t\t<span class=\"vhead\">Advantages:<\/span>\n\t\t\t\t\t<ul class=\"vlist\">\n\t\t\t\t\t\t<li><span>Reliable and easy protocol<br \/><\/span><\/li><li><span>Improves library quality through repair of damages from FFPE fixation<br \/><\/span><\/li><li><span>No change\/bias compared to untreated sequence<br \/><\/span><\/li><li><span>Application directly prior to the Library Prep step, therefore compatible with all platforms and workflows<\/span><\/li>\n\t\t\t\t\t<\/ul>\n\t\t\t<\/div>[\/vc_column][\/vc_row]<span data-mce-type=\"bookmark\" style=\"display: inline-block; width: 0px; overflow: hidden; line-height: 0;\" class=\"mce_SELRES_end\">\ufeff<\/span>[vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]<span class=\"bildunterschrift\">Figure: The effect of DNA repair on the percentage of mappable reads. Reads were mapped to the human genome (hg19) using BWA (1).<\/span>[\/vc_column_text][vc_single_image image=&#8221;5392&#8243; img_size=&#8221;medium&#8221; img_link_large=&#8221;yes&#8221;][\/vc_column][vc_column width=&#8221;1\/2&#8243;][vc_column_text]<span class=\"bildunterschrift\">Figure: An example of Agilent Bioanalyzer\u00ae traces of libraries prepared from stomach tumor FFPE DNA that was treated with the FFPE DNA Repair Mix, or was untreated, before library construction.\u00a0Yield improvements of 10% to 458% have been observed.\u00a0<\/span>[\/vc_column_text][vc_single_image image=&#8221;5394&#8243; img_size=&#8221;medium&#8221; img_link_large=&#8221;yes&#8221;][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][vc_column_text]<\/p>\n<table style=\"width: 760px;\" border=\"0\" cellpadding=\"5\">\n<tbody>\n<tr style=\"background-color: #f3b281;\">\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"396\" height=\"40px\">PRODUCT<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"80\">REF.<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"132\">SIZE<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" colspan=\"1\" width=\"152\">PRICE<\/th>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">NEBNext FFPE DNA Repair Mix<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M6630 S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">24 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/m6630s\"><img decoding=\"async\" class=\"aligncenter\" src=\"http:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"NEB Shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">NEBNext FFPE DNA Repair Mix<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M6630 L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">96 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/m6630l\"><img decoding=\"async\" class=\"aligncenter\" src=\"http:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"NEB Shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>\ufeff[vc_row][vc_column width=&#8221;2\/3&#8243;][vc_column_text]Archiving of clinical materials as Formalin-Fixed, Paraffin-Embedded (FFPE) samples is a common practice. However, the methods used for fixation and storage significantly damage and compromise the quality of nucleic acids from these samples. As a result, it can be challenging to obtain useful information, including high-quality sequence data, especially when sample amounts are limited&#8230;.  <a class=\"excerpt-read-more\" href=\"https:\/\/www.neb-online.fr\/en\/next-generation-sequencing\/nebnext-ffpe-dna-repair-mix\/\" title=\"Read NEBNext FFPE DNA Repair Mix\">Read more &raquo;<\/a><\/p>\n","protected":false},"author":15,"featured_media":1788,"parent":2170,"menu_order":0,"comment_status":"open","ping_status":"closed","template":"page-sidebar-next-generation-sequencing.php","meta":{"footnotes":""},"class_list":["post-5399","page","type-page","status-publish","has-post-thumbnail","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.6 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>NEBNext FFPE DNA Repair Mix - New England Biolabs France<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.neb-online.fr\/en\/next-generation-sequencing\/nebnext-ffpe-dna-repair-mix\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"NEBNext FFPE DNA Repair Mix - New England Biolabs France\" \/>\n<meta property=\"og:description\" content=\"\ufeff[vc_row][vc_column width=&#8221;2\/3&#8243;][vc_column_text]Archiving of clinical materials as Formalin-Fixed, Paraffin-Embedded (FFPE) samples is a common practice. However, the methods used for fixation and storage significantly damage and compromise the quality of nucleic acids from these samples. As a result, it can be challenging to obtain useful information, including high-quality sequence data, especially when sample amounts are limited.... Read more &raquo;\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.neb-online.fr\/en\/next-generation-sequencing\/nebnext-ffpe-dna-repair-mix\/\" \/>\n<meta property=\"og:site_name\" content=\"New England Biolabs France\" \/>\n<meta property=\"article:modified_time\" content=\"2020-04-16T21:13:06+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.neb-online.fr\/wp-content\/uploads\/2015\/04\/NEB_nextgenseq_nebnextultrax760px.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"760\" \/>\n\t<meta property=\"og:image:height\" content=\"200\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/www.neb-online.fr\\\/en\\\/next-generation-sequencing\\\/nebnext-ffpe-dna-repair-mix\\\/\",\"url\":\"https:\\\/\\\/www.neb-online.fr\\\/en\\\/next-generation-sequencing\\\/nebnext-ffpe-dna-repair-mix\\\/\",\"name\":\"NEBNext FFPE DNA Repair Mix - New England Biolabs France\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/www.neb-online.fr\\\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\\\/\\\/www.neb-online.fr\\\/en\\\/next-generation-sequencing\\\/nebnext-ffpe-dna-repair-mix\\\/#primaryimage\"},\"image\":{\"@id\":\"https:\\\/\\\/www.neb-online.fr\\\/en\\\/next-generation-sequencing\\\/nebnext-ffpe-dna-repair-mix\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/www.neb-online.fr\\\/wp-content\\\/uploads\\\/2015\\\/04\\\/NEB_nextgenseq_nebnextultrax760px.jpg\",\"datePublished\":\"2015-07-22T11:59:38+00:00\",\"dateModified\":\"2020-04-16T21:13:06+00:00\",\"breadcrumb\":{\"@id\":\"https:\\\/\\\/www.neb-online.fr\\\/en\\\/next-generation-sequencing\\\/nebnext-ffpe-dna-repair-mix\\\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/www.neb-online.fr\\\/en\\\/next-generation-sequencing\\\/nebnext-ffpe-dna-repair-mix\\\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\\\/\\\/www.neb-online.fr\\\/en\\\/next-generation-sequencing\\\/nebnext-ffpe-dna-repair-mix\\\/#primaryimage\",\"url\":\"https:\\\/\\\/www.neb-online.fr\\\/wp-content\\\/uploads\\\/2015\\\/04\\\/NEB_nextgenseq_nebnextultrax760px.jpg\",\"contentUrl\":\"https:\\\/\\\/www.neb-online.fr\\\/wp-content\\\/uploads\\\/2015\\\/04\\\/NEB_nextgenseq_nebnextultrax760px.jpg\",\"width\":760,\"height\":200,\"caption\":\"NEBNext Ultra Banner\"},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/www.neb-online.fr\\\/en\\\/next-generation-sequencing\\\/nebnext-ffpe-dna-repair-mix\\\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Next Generation Sequencing\",\"item\":\"https:\\\/\\\/www.neb-online.fr\\\/en\\\/next-generation-sequencing\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"NEBNext FFPE DNA Repair Mix\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/www.neb-online.fr\\\/#website\",\"url\":\"https:\\\/\\\/www.neb-online.fr\\\/\",\"name\":\"New England Biolabs France\",\"description\":\"be INSPIRED, drive DISCOVERY, stay GENUINE\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/www.neb-online.fr\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"NEBNext FFPE DNA Repair Mix - New England Biolabs France","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.neb-online.fr\/en\/next-generation-sequencing\/nebnext-ffpe-dna-repair-mix\/","og_locale":"en_US","og_type":"article","og_title":"NEBNext FFPE DNA Repair Mix - New England Biolabs France","og_description":"\ufeff[vc_row][vc_column width=&#8221;2\/3&#8243;][vc_column_text]Archiving of clinical materials as Formalin-Fixed, Paraffin-Embedded (FFPE) samples is a common practice. 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