{"id":5679,"date":"2015-10-21T19:46:00","date_gmt":"2015-10-21T17:46:00","guid":{"rendered":"http:\/\/www.neb-online.fr\/en\/?page_id=5679"},"modified":"2022-11-07T16:28:58","modified_gmt":"2022-11-07T15:28:58","slug":"nebnext-automation","status":"publish","type":"page","link":"https:\/\/www.neb-online.fr\/en\/next-generation-sequencing\/nebnext-automation\/","title":{"rendered":"NEBNext Automation"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row][vc_column][vc_column_text]As sequencing instrument capacity is increasing, and the ever-growing range of sequencing applications is demanding expanded sample preparation throughputs, many NGS users are turning to automation. Manual preparation and quantitation of large numbers of libraries can result in a sequencing workflow bottleneck, and automation of these protocols is an important, and increasingly popular, option.<\/p>\n<p><strong>Advantages of automation include:<\/strong><\/p>\n<ul>\n<li>Increased throughput<\/li>\n<li>Improved reproducibility<\/li>\n<li>Increased speed<\/li>\n<li>Reduced hands-on time<\/li>\n<li>Reduced Repetitive Strain Injury (RSI) incidence<\/li>\n<\/ul>\n<p><strong>NEBNext<sup>\u00ae<\/sup> products are developed with automation in mind:<\/strong><\/p>\n<ul>\n<li>Fewer components simplify automation setup<\/li>\n<li>Streamlined workflows minimize tip consumption and chances for pipetting errors<\/li>\n<li>Less clean-up bead consumption results in a lower cost-per-reaction<\/li>\n<li>Low input amount capability (ng for DNA\/RNA, pg for ChIP) improves sequencing results with less sample<\/li>\n<li>Robust reagents improve overall library yields<\/li>\n<li>Sufficient kit component fill volumes for automation protocols improves usability<\/li>\n<\/ul>\n<p>NEBNext kits have been automated by multiple automation platform providers, including Beckman Coulter<sup>\u00ae<\/sup>, Hamilton<sup>\u00ae<\/sup> and PerkinElmer<sup>\u00ae<\/sup>.[\/vc_column_text][vc_separator][\/vc_column][\/vc_row][vc_row css=&#8221;.vc_custom_1503413671261{margin-right: 0px !important;margin-left: 0px !important;border-top-width: 2px !important;border-bottom-width: 2px !important;background-color: #f4f1e7 !important;border-top-color: #eb7e2d !important;border-top-style: solid !important;border-bottom-color: #eb7e2d !important;border-bottom-style: solid !important;border-radius: 2px !important;}&#8221;][vc_column width=&#8221;1\/2&#8243;][vc_single_image image=&#8221;5682&#8243; img_size=&#8221;full&#8221; alignment=&#8221;center&#8221;][vc_column_text]Learn how Biomek automation of NEBNext RNA library preparation enables high-throughput, reproducible production of libraries with high yield and complexity. In this webinar Fiona Stewart from New England Biolabs and Zach Smith from Beckman Coulter describe strand-specific RNA library preparation using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina and discuss Biomek automation of this method.[\/vc_column_text]<a class=\"btn btn-standard form-btn\"  href=\"https:\/\/www.neb.com\/tools-and-resources\/video-library?device=modal&amp;videoid={3E3A23E4-881D-4B88-9F50-FDA007E788AA}\" style=\"\">View Video<\/a>[\/vc_column][vc_column width=&#8221;1\/2&#8243;][vc_single_image image=&#8221;5681&#8243; img_size=&#8221;full&#8221; alignment=&#8221;center&#8221;][vc_column_text]Learn how Biomek automation of NEBNext DNA library preparation for Ion Torrent enables high-throughput, reproducible production of libraries with high yield and complexity. In this webinar Joanna Hamilton from Dartmouth Genomics &amp; Molecular Biology Shared Resource, Lynne Apone from New England Biolabs and Zach Smith from Beckman Coulter describe the NEBNext Fast Fragmentation &amp; DNA Library Prep kit for Ion Torrent, automation of this method and implementation in multiple workflows in a sequencing core lab.[\/vc_column_text]<a class=\"btn btn-standard form-btn\"  href=\"https:\/\/www.neb.com\/tools-and-resources\/video-library?device=modal&amp;videoid={320B4B35-91B0-4E03-8C40-B9F8A623AFEF}\" style=\"\">View Video<\/a>[\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]<strong><em>E. coli<\/em> K12 gDNA control libraries constructed with the NEBNext Ultra DNA automated method on the Biomek<sup>\u00ae<\/sup> platform<\/strong>[\/vc_column_text][vc_single_image image=&#8221;5715&#8243; img_size=&#8221;medium&#8221; alignment=&#8221;center&#8221; img_link_large=&#8221;yes&#8221;][vc_column_text]<span class=\"bildunterschrift\">Traces from the Agilent<sup>\u00ae<\/sup> 2200 TapeStation, using D1000 ScreenTape, of six <em>E. coli<\/em> K12 control libraries show consistent performance. For more information see the Application Note <a class=\"ga-event\" href=\"https:\/\/www.neb.com\/%7E\/media\/NebUs\/Files\/Application%20Notes\/A%20Library%20Prep%20Kit%20for%20Illumina%20on%20the%20Beckman%20Coulter%20Biomek%20FXP%20Automated%20Liquid%20Handler.pdf\" data-ga-label=\"https:\/\/www.neb.com\/~\/media\/NebUs\/Files\/Application%20Notes\/A%20Library%20Prep%20Kit%20for%20Illumina%20on%20the%20Beckman%20Coulter%20Biomek%20FXP%20Automated%20Liquid%20Handler.pdf\" data-ga-action=\"Download\" data-ga-category=\"Download: PDF\">Automated DNA Library Construction Using the NEBNext Ultra DNA Library Prep Kit for Illumina<sup>\u00ae<\/sup> on the Beckman Coulter Biomek FXP Automated Liquid Handler<\/a><\/span>[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/2&#8243;][vc_column_text]<strong>Checker Board Test for Cross-Contamination with the Hamilton STARlet\u2122 platform<\/strong>[\/vc_column_text][vc_single_image image=&#8221;5716&#8243; img_size=&#8221;medium&#8221; alignment=&#8221;center&#8221; img_link_large=&#8221;yes&#8221;][vc_column_text]<span class=\"bildunterschrift\">Bioanalyzer DNA 1000 traces from wells A1-A6 of the checker board test using <em>E. coli<\/em> DNA samples and water samples. The representative data of a 48 sample run reveal no cross contamination between individual wells. For more information see the Application Note <a class=\"ga-event\" href=\"https:\/\/www.neb.com\/%7E\/media\/NebUs\/Files\/Application%20Notes\/NEBNext_ULTRA_DNA_Automation.pdf\" data-ga-label=\"https:\/\/www.neb.com\/~\/media\/NebUs\/Files\/Application%20Notes\/NEBNext_ULTRA_DNA_Automation.pdf\" data-ga-action=\"Download\" data-ga-category=\"Download: PDF\">Automated Library Preparation using NEBNext<sup>\u00ae<\/sup> Ultra\u2122 DNA Library Prep Kit for Illumina<sup>\u00ae<\/sup> (E7370) on the HAMILTON STAR Line<\/a><\/span>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<\/p>\n<div class=\"vc_row wpb_row vc_row-fluid\">\n<div class=\"wpb_column vc_column_container vc_col-sm-12\">\n<div class=\"vc_column-inner \">\n<div class=\"wpb_wrapper\">\n<div class=\"wpb_text_column wpb_content_element \">\n<div class=\"wpb_wrapper\">\n<h2>Application Notes on Automation for download:<\/h2>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"vc_row wpb_row vc_row-fluid\">\n<div class=\"wpb_column vc_column_container vc_col-sm-12\">\n<div class=\"vc_column-inner \">\n<div class=\"wpb_wrapper\">\n<div class=\"vc_row wpb_row vc_inner vc_row-fluid\">\n<div class=\"wpb_column vc_column_container vc_col-sm-8\">\n<div class=\"vc_column-inner \">\n<div class=\"wpb_wrapper\">\n<div class=\"wpb_text_column wpb_content_element \">\n<div class=\"wpb_wrapper\">\n<p><strong>Beckman Coulter:<\/strong> Automated DNA Library Construction Using the NEBNext Ultra DNA Library Prep Kit for Illumina\u00ae on the Beckman Coulter Biomek FXP Automated Liquid Handler<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"wpb_column vc_column_container vc_col-sm-4\">\n<div class=\"vc_column-inner \">\n<div class=\"wpb_wrapper\"><a class=\"btn btn-standard form-btn\" style=\"margin-left: auto; margin-right: auto;\" href=\"https:\/\/www.neb.com\/~\/media\/NebUs\/Files\/Application%20Notes\/A%20Library%20Prep%20Kit%20for%20Illumina%20on%20the%20Beckman%20Coulter%20Biomek%20FXP%20Automated%20Liquid%20Handler.pdf\" target=\"_blank\" rel=\"noopener\">PDF-Download<\/a><\/div>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"vc_row wpb_row vc_inner vc_row-fluid\">\n<div class=\"wpb_column vc_column_container vc_col-sm-8\">\n<div class=\"vc_column-inner \">\n<div class=\"wpb_wrapper\">\n<div class=\"wpb_text_column wpb_content_element \">\n<div class=\"wpb_wrapper\">\n<p><strong>Beckman Coulter:<\/strong> Automated Directional RNASeq Library Preparation using the Biomek FXP Liquid Handler<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"wpb_column vc_column_container vc_col-sm-4\">\n<div class=\"vc_column-inner \">\n<div class=\"wpb_wrapper\"><a class=\"btn btn-standard form-btn\" style=\"margin-left: auto; margin-right: auto;\" href=\"https:\/\/www.neb.com\/-\/media\/nebus\/files\/application-notes\/appnote_automated_directional_rnaseq_library_prep_using_biomek_fxp_liquid_handler.pdf?rev=acfc462695de4c59b64442ba65b54758\" target=\"_blank\" rel=\"noopener\">PDF-Download<\/a><\/div>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"vc_row wpb_row vc_inner vc_row-fluid\">\n<div class=\"wpb_column vc_column_container vc_col-sm-8\">\n<div class=\"vc_column-inner \">\n<div class=\"wpb_wrapper\">\n<div class=\"wpb_text_column wpb_content_element \">\n<div class=\"wpb_wrapper\">\n<p><strong>Hamilton:<\/strong> Automated Library Preparation using NEBNext\u00ae Ultra\u2122 DNA Library Prep Kit for Illumina\u00ae (E7370) on the HAMILTON\u00ae STAR\u2122 Line<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"wpb_column vc_column_container vc_col-sm-4\">\n<div class=\"vc_column-inner \">\n<div class=\"wpb_wrapper\"><a class=\"btn btn-standard form-btn\" style=\"margin-left: auto; margin-right: auto;\" href=\"https:\/\/www.neb.com\/~\/media\/NebUs\/Files\/Application%20Notes\/NEBNext_ULTRA_DNA_Automation.pdf\" target=\"_blank\" rel=\"noopener\">PDF-Download<\/a><\/div>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"vc_row wpb_row vc_inner vc_row-fluid\">\n<div class=\"wpb_column vc_column_container vc_col-sm-8\">\n<div class=\"vc_column-inner \">\n<div class=\"wpb_wrapper\">\n<div class=\"wpb_text_column wpb_content_element \">\n<div class=\"wpb_wrapper\">\n<p><strong>Sage Science:<\/strong> Automated size selection of NEBNext Small RNA libraries with the Sage Pippin Prep<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"wpb_column vc_column_container vc_col-sm-4\">\n<div class=\"vc_column-inner \">\n<div class=\"wpb_wrapper\"><a class=\"btn btn-standard form-btn\" style=\"margin-right: auto;\" href=\"https:\/\/www.neb.com\/-\/media\/nebus\/files\/application-notes\/appnote_nebnext_small_rna_size_selection.pdf?rev=f4a21d6cd91e46a6a4d8c76639d6b2b3\" target=\"_blank\" rel=\"noopener\">PDF Download<\/a><\/div>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"vc_row wpb_row vc_inner vc_row-fluid\">\n<div class=\"wpb_column vc_column_container vc_col-sm-8\">\n<div class=\"vc_column-inner \">\n<div class=\"wpb_wrapper\">\n<div class=\"wpb_text_column wpb_content_element \">\n<div class=\"wpb_wrapper\">\n<p><strong>Tecan:<\/strong> Automated NEBNext\u00ae Ultra\u2122 II DNA Library Preparation for Illumina\u00ae<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"wpb_column vc_column_container vc_col-sm-4\">\n<div class=\"vc_column-inner \">\n<div class=\"wpb_wrapper\">\n<p><a class=\"btn btn-standard form-btn\" style=\"margin-left: auto; margin-right: auto;\" href=\"https:\/\/international.neb.com\/-\/media\/nebus\/files\/application-notes\/appnote_automated_nebnext_ultra_ii_dna_library_preparation_for_illumina.pdf?rev=72f17244fecd499aae4fd83e7e5b3e80\" target=\"_blank\" rel=\"noopener\">PDF Download<\/a><\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<p>[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row][vc_column][vc_column_text]As sequencing instrument capacity is increasing, and the ever-growing range of sequencing applications is demanding expanded sample preparation throughputs, many NGS users are turning to automation. Manual preparation and quantitation of large numbers of libraries can result in a sequencing workflow bottleneck, and automation of these protocols is an important, and increasingly popular, option. Advantages&#8230;  <a class=\"excerpt-read-more\" href=\"https:\/\/www.neb-online.fr\/en\/next-generation-sequencing\/nebnext-automation\/\" title=\"Read NEBNext Automation\">Read more &raquo;<\/a><\/p>\n","protected":false},"author":15,"featured_media":9555,"parent":2170,"menu_order":0,"comment_status":"open","ping_status":"closed","template":"page-sidebar-next-generation-sequencing.php","meta":{"footnotes":""},"class_list":["post-5679","page","type-page","status-publish","has-post-thumbnail","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.7 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>NEBNext Automation - New England Biolabs France<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.neb-online.fr\/en\/next-generation-sequencing\/nebnext-automation\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"NEBNext Automation - New England Biolabs France\" \/>\n<meta property=\"og:description\" content=\"[vc_row][vc_column][vc_column_text]As sequencing instrument capacity is increasing, and the ever-growing range of sequencing applications is demanding expanded sample preparation throughputs, many NGS users are turning to automation. 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