{"id":6248,"date":"2015-09-14T11:51:18","date_gmt":"2015-09-14T09:51:18","guid":{"rendered":"http:\/\/www.neb-online.fr\/en\/neb\/pcr-and-dna-amplification\/reverse-transcription-cdna-synthesis\/onetaq-one-step\/"},"modified":"2015-12-14T17:24:41","modified_gmt":"2015-12-14T16:24:41","slug":"onetaq-one-step","status":"publish","type":"page","link":"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/reverse-transcription-cdna-synthesis\/onetaq-one-step\/","title":{"rendered":"OneTaq One-Step RT-PCR Kit"},"content":{"rendered":"

[vc_row][vc_column width=”1\/2″][vc_column_text]The OneTaq<\/em>\u00a0One-Step RT-PCR Kit<\/strong> offers sensitive and robust end-point detection of RNA templates. cDNA synthesis and PCR amplification steps are performed in a single reaction using gene-specific primers, resulting in a streamlined RT-PCR protocol.<\/p>\n

The kit combines three optimized mixes: OneTaq<\/em>\u00a0One-Step Enzyme Mix, OneTaq<\/em>\u00a0One-Step Reaction Mix and OneTaq<\/em>\u00a0One-Step Quick-Load Reaction Mix. The Enzyme Mix combines ProtoScript II Reverse Transcriptase, Murine RNase Inhibitor and OneTaq<\/em>\u00a0Hot Start DNA Polymerase. ProtoScript II Reverse Transcriptase is a mutant M-MuLV reverse transcriptase with reduced RNase H activity and increased thermostability. OneTaq<\/em>\u00a0Hot Start DNA Polymerase is mixture of a Hot Start Taq DNA Polymerase combined with a proof-reading DNA polymerase, resulting in high-yield amplification with minimal optimization. The OneTaq\u00a0<\/em>One-Step RT-PCR Kit is capable of amplifying long transcripts up to 9 kb in length.<\/p>\n

Two optimized reaction mixes are included, OneTaq<\/em>\u00a0One-Step Reaction Mix and Quick-Load OneTaq<\/em>One-Step Reaction Mix. The reaction mixes offer robust conditions for both cDNA synthesis and PCR amplification. The unique OneTaq<\/em>\u00a0Quick-Load One-Step Reaction Mix contains additional dyes, offering color indication for reaction setup as well as direct gel loading.<\/p>\n

Both total RNA and mRNA can be used as template. The kit can detect a GAPDH target as low as 0.1 pg per reaction. It can routinely detect RNA targets up to 9 kb. The OneTaq<\/em>\u00a0One-Step RT-PCR Kit is capable of multiplex detection of two or three targets.[\/vc_column_text][\/vc_column][vc_column width=”1\/2″]

\n\t\t\t\tAdvantages:<\/span>\n\t\t\t\t\t