{"id":6403,"date":"2015-10-21T18:43:32","date_gmt":"2015-10-21T16:43:32","guid":{"rendered":"http:\/\/www.neb-online.fr\/en\/?page_id=6403"},"modified":"2020-05-27T15:54:45","modified_gmt":"2020-05-27T13:54:45","slug":"neb-tools","status":"publish","type":"page","link":"https:\/\/www.neb-online.fr\/en\/technical-resources-neb\/neb-tools\/","title":{"rendered":"NEB Web Tools"},"content":{"rendered":"

[vc_row][vc_column el_class=”tools-slider”][vc_images_carousel images=”7250,2007,2008,2015,2017,2018,2006,2013,2014,2016,2009,2053,2011,2012,14375,14377,14379,14381,14383,14385″ img_size=”full” onclick=”custom_link” custom_links_target=”_blank” slides_per_view=”8″ custom_links=”#E-8_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” el_class=”tools-slider-8items”][vc_images_carousel images=”7250,2007,2008,2015,2017,2018,2006,2013,2014,2016,2009,2053,2011,2012,14375,14377,14379,14381,14383,14385″ img_size=”full” onclick=”custom_link” custom_links_target=”_blank” slides_per_view=”4″ custom_links=”#E-8_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” el_class=”tools-slider-4items”][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][\/vc_column][\/vc_row][vc_row][vc_column width=”1\/4″][vc_column_text]\"NEBNext_WebTool_62x64\"<\/a>[\/vc_column_text][\/vc_column][vc_column width=”3\/4″][vc_column_text]NEBNext Selector<\/a><\/p>\n

NEBNext Selector is a guide for selecting appropriate products for NextGen sequencing workflows.[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][\/vc_column][\/vc_row][vc_row][vc_column width=”1\/4″][vc_column_text]\"NEB_Cutter150\"<\/a>[\/vc_column_text][\/vc_column][vc_column width=”3\/4″][vc_column_text]NEBcutter V2.0<\/a><\/p>\n

Use this tool to identify the restriction sites within your DNA sequence. Choose between Type II and commercially available Type III restriction enzymes<\/a> to digest your DNA. NEBcutter\u00ae V2.0 will indicate cut frequency and methylation state sensitivity.[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][\/vc_column][\/vc_row][vc_row][vc_column width=”1\/4″][vc_column_text]\"NEB_DoubleDigestFinder150\"<\/a>[\/vc_column_text][\/vc_column][vc_column width=”3\/4″][vc_column_text]Double Digest Finder<\/a><\/p>\n

Use this tool to guide your reaction buffer selection when setting up double-digests, a common timesaving procedure. Choosing the right buffers will help you to avoid star activity and loss of product.[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][\/vc_column][\/vc_row][vc_row][vc_column width=”1\/4″][vc_column_text]\"NEB_TmCalculator150\"<\/a>[\/vc_column_text][\/vc_column][vc_column width=”3\/4″][vc_column_text]Tm Calculator<\/a><\/p>\n

Use this tool when designing PCR reaction protocols to help determine the optimal annealing temperature for your amplicon. Simply input your DNA polymerase, primer concentration and your primer sequence and the Tm Calculator will guide you to successful reaction conditions.[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][\/vc_column][\/vc_row][vc_row][vc_column width=”1\/4″][vc_column_text]\"NEBioCalculator150\"<\/a>[\/vc_column_text][\/vc_column][vc_column width=”3\/4″][vc_column_text]NEBioCalculator<\/a><\/p>\n

Use this tool for your scientific calculations and conversions for DNA and RNA. Options include conversion of mass to moles, ligation amounts, conversion of OD to concentration, dilution and molarity.[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][\/vc_column][\/vc_row][vc_row][vc_column width=”1\/4″][vc_column_text]\"NEBuilder\"<\/a>[\/vc_column_text][\/vc_column][vc_column width=”3\/4″][vc_column_text]NEBuilder\u00a0Assembly Tool<\/a><\/p>\n

NEBuilder\u00ae Assembly Tool can be used to design primers for your NEBuilder or Gibson Assembly <\/a>reaction, based on the entered fragment sequences and the polymerase being used for amplification.[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][\/vc_column][\/vc_row][vc_row][vc_column width=”1\/4″][vc_column_text]\"icon_nebcloner_tool_150px\"<\/a>[\/vc_column_text][\/vc_column][vc_column width=”3\/4″][vc_column_text]NEBCloner<\/a><\/p>\n

Use this tool to find the right products and protocols for each step (digestion, end modification, ligation and transformation) of your next traditional cloning experiment. Also, find other relevant tools and resources to enable protocol optimization.[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][\/vc_column][\/vc_row][vc_row][vc_column width=”1\/4″][vc_column_text]\"NEB_Enzymfinder150\"<\/a>[\/vc_column_text][\/vc_column][vc_column width=”3\/4″][vc_column_text]Enzyme Finder<\/a><\/p>\n

Use this tool to select restriction enzymes<\/a> by name, sequence, overhang or type. Enter your sequence using single letter code nomenclature, and Enzyme Finder will identify the right enzyme for the job.[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][\/vc_column][\/vc_row][vc_row][vc_column width=”1\/4″][vc_column_text]\"NEB_SequenceMap150\"<\/a>[\/vc_column_text][\/vc_column][vc_column width=”3\/4″][vc_column_text]DNA Sequences & Maps Tools<\/a><\/p>\n

Use this tool to find the nucleotide sequence files for commonly used molecular biology tools, including plasmid, viral and bacteriophage vectors.[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][\/vc_column][\/vc_row][vc_row][vc_column width=”1\/4″][vc_column_text]\"NEB_PCR_Selector150\"<\/a>[\/vc_column_text][\/vc_column][vc_column width=”3\/4″][vc_column_text]PCR Selection Tool<\/a><\/p>\n

Use this tool to help select the right DNA polymerase for your PCR setup. Whether your amplicon is long, complex, GC-rich or present in a single copy, the PCR selection tool will identify the perfect DNA polymerase for your reaction.[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][\/vc_column][\/vc_row][vc_row][vc_column width=”1\/4″][vc_column_text]\"Golden<\/a>[\/vc_column_text][\/vc_column][vc_column width=”3\/4″][vc_column_text]NEB Golden Gate Assembly Tool<\/a><\/p>\n

Use this tool to assist with in silico DNA construct design for Golden Gate DNA assembly<\/a>. It enables the accurate design of primers with appropriate type IIS restriction sites and overlaps, quick import of sequences in many formats and export of the final assembly, primers and settings.[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][\/vc_column][\/vc_row][vc_row][vc_column width=”1\/4″][vc_column_text]\"NEBaseChanger150\"<\/a>[\/vc_column_text][\/vc_column][vc_column width=”3\/4″][vc_column_text]NEBaseChanger<\/a><\/p>\n

NEBaseChanger can be used to design primers specific to the mutagenesis experiment you are performing using the Q5\u00ae Site-Directed Mutagenesis Kit<\/a>. This tool will also calculate a recommended custom annealing temperature based on the sequence of the primers by taking into account any mismatches.[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][\/vc_column][\/vc_row][vc_row][vc_column width=”1\/4″][vc_column_text]\"NEB_ReBase150\"<\/a>[\/vc_column_text][\/vc_column][vc_column width=”3\/4″][vc_column_text]REBASE<\/a><\/p>\n

Use this tool as a guide to the ever-changing landscape of restriction enzymes. REBASE, the Restriction Enzyme DataBASE, is a dynamic, curated database of restriction enzymes and related proteins.[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][\/vc_column][\/vc_row][vc_row][vc_column width=”1\/4″][vc_column_text]\"Icon<\/a>[\/vc_column_text][\/vc_column][vc_column width=”3\/4″][vc_column_text]PolBase<\/a><\/p>\n

Polbase is a repository of biochemical, genetic, and structural information about DNA Polymerases.[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][\/vc_column][\/vc_row][vc_row][vc_column width=”1\/4″][vc_column_text]\"\"<\/a>[\/vc_column_text][\/vc_column][vc_column width=”3\/4″][vc_column_text]Competitor Cross-Reference Tools<\/a><\/p>\n

Use this tool to select another company’s product and find out which NEB product is compatible. Choose either the competitor\u2019s product name or catalog number from the available selections, and this tool will identify the recommended NEB product.[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][\/vc_column][\/vc_row][vc_row][vc_column width=”1\/4″][vc_column_text]\"\"<\/a>[\/vc_column_text][\/vc_column][vc_column width=”3\/4″][vc_column_text]Exo Selector<\/a><\/p>\n

Use this tool to simplify the process of selecting the appropriate exonucleases for use in your nucleic acid digestion workflows. The tool guides you to product recommendations based on your answers to a few simple questions.<\/div>\n

[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][\/vc_column][\/vc_row][vc_row][vc_column width=”1\/4″][vc_column_text]\"\"<\/a>[\/vc_column_text][\/vc_column][vc_column width=”3\/4″][vc_column_text]Glycan Analyzer<\/a><\/p>\n

Use this tool to interpret Ultra or High Pressure liquid chromatography (UPLC\/HPLC) N-glycan profiles following exoglycosidase digestions.<\/div>\n

[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][\/vc_column][\/vc_row][vc_row][vc_column width=”1\/4″][vc_column_text]\"\"<\/a>[\/vc_column_text][\/vc_column][vc_column width=”3\/4″][vc_column_text]PCR Fidelity Estimator<\/a><\/p>\n

Estimate the percentage of correct DNA copies (those without base substitution errors) per cycle of PCR for selected DNA polymerases.<\/div>\n

[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][\/vc_column][\/vc_row][vc_row][vc_column width=”1\/4″][vc_column_text]\"\"<\/a>[\/vc_column_text][\/vc_column][vc_column width=”3\/4″][vc_column_text]Read Coverage Calculator<\/a><\/p>\n

This tool allows for easy calculation of values associated with read coverage in NGS protocols.<\/div>\n

[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][\/vc_column][\/vc_row][vc_row][vc_column width=”1\/4″][vc_column_text]\"\"<\/a>[\/vc_column_text][\/vc_column][vc_column width=”3\/4″][vc_column_text]Thermostable Ligase Reaction Temperature Calculator<\/a><\/p>\n

This tool will estimate an optimal reaction temperature to minimize mismatch for thermostable ligation of two adjacent ssDNA probes annealed to a template.<\/div>\n

[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]In case of any question regarding the use of the NEB tools or NEB products please contact the NEB Technical Support<\/a>.[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"

[vc_row][vc_column el_class=”tools-slider”][vc_images_carousel images=”7250,2007,2008,2015,2017,2018,2006,2013,2014,2016,2009,2053,2011,2012,14375,14377,14379,14381,14383,14385″ img_size=”full” onclick=”custom_link” custom_links_target=”_blank” slides_per_view=”8″ custom_links=”#E-8_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” el_class=”tools-slider-8items”][vc_images_carousel images=”7250,2007,2008,2015,2017,2018,2006,2013,2014,2016,2009,2053,2011,2012,14375,14377,14379,14381,14383,14385″ img_size=”full” onclick=”custom_link” custom_links_target=”_blank” slides_per_view=”4″ custom_links=”#E-8_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” el_class=”tools-slider-4items”][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][\/vc_column][\/vc_row][vc_row][vc_column width=”1\/4″][vc_column_text][\/vc_column_text][\/vc_column][vc_column width=”3\/4″][vc_column_text]NEBNext Selector NEBNext Selector is a guide for selecting appropriate products for NextGen sequencing workflows.[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][\/vc_column][\/vc_row][vc_row][vc_column width=”1\/4″][vc_column_text][\/vc_column_text][\/vc_column][vc_column width=”3\/4″][vc_column_text]NEBcutter V2.0 Use this tool to identify the restriction sites within your DNA sequence. 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Choose between Type II and commercially available Type... 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