{"id":8750,"date":"2017-01-12T12:52:29","date_gmt":"2017-01-12T11:52:29","guid":{"rendered":"http:\/\/www.neb-online.fr\/en\/?page_id=8750"},"modified":"2022-11-14T14:25:30","modified_gmt":"2022-11-14T13:25:30","slug":"luna-universal-qpcr-rt-qpcr-optimize-rt-qpcr-with-unique-warmstart-technology","status":"publish","type":"page","link":"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/luna-universal-qpcr-rt-qpcr\/luna-universal-qpcr-rt-qpcr-optimize-rt-qpcr-with-unique-warmstart-technology\/","title":{"rendered":"Luna\u00ae \u2013 Optimize RT-qPCR with unique WarmStart\u00ae technology"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row][vc_column][vc_column_text]The Luna RT-qPCR kits contain a novel, in <em>silico<\/em>-designed reverse transcriptase (RT) engineered for improved performance. Both the Luna WarmStart Reverse Transcriptase and Hot Start <em>Taq<\/em> DNA Polymerase, included in these kits, utilize a temperature-sensitive, reversible aptamer, which inhibits activity below 45\u00b0C. This enables room temperature reaction setup and prevents undesired non-specific activity. Furthermore, the WarmStart RT has increased thermostability, improving performance at higher reaction temperatures.[\/vc_column_text][vc_separator][vc_column_text]<\/p>\n<h2>Improve performance with our novel, thermostable reverse transcriptase<\/h2>\n<ul>\n<li>Robust RT delivers improved specificity, sensitivity and efficiency<\/li>\n<li>Increased thermostability adds flexibility to reaction setup and improved specificity<\/li>\n<\/ul>\n<p>[\/vc_column_text][vc_column_text]<strong>Luna WarmStart Reverse Transcriptase exhibits exceptional sensitivity, reproducibility and RT-qPCR performance<\/strong>[\/vc_column_text][vc_single_image image=&#8221;8689&#8243; img_size=&#8221;full&#8221; onclick=&#8221;img_link_large&#8221;][vc_column_text]<span class=\"bildunterschrift\">RT-qPCR targeting human GAPDH was performed using the Luna Universal One-Step RT-qPCR Kit, featuring Luna WarmStart Reverse Transcriptase, over an 8-log range of input template concentrations (0.1 pg \u2013 1 \u03bcg Jurkat total RNA) with 8 replicates at each concentration. NTC = non-template control. <\/span>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text][\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][vc_column_text]<\/p>\n<h2>Achieve better control and better results: WarmStart Luna RT paired with Hot Start <i>Taq<\/i><\/h2>\n<ul>\n<li>WarmStart\/Hot Start technology ensures tight control and prevents spurious amplification<\/li>\n<li>Unique WarmStart technology is not available with other non-NEB commercially-available products<\/li>\n<\/ul>\n<p>[\/vc_column_text][vc_column_text]<strong>WarmStart Luna RT prevents spurious amplification resulting from room-temperature  pre-incubation<\/strong>[\/vc_column_text][vc_single_image image=&#8221;8688&#8243; img_size=&#8221;full&#8221; onclick=&#8221;img_link_large&#8221;][vc_column_text]<span class=\"bildunterschrift\">RT-qPCR targeting human ribosomal protein L32 was performed before and after a 24-hour incubation at room temperature, with triplicate reactions for a 5-log range of input human (Jurkat) total RNA and a non-template control. The Luna Universal One-Step RT-qPCR Kit featuring Luna WarmStart Reverse Transcriptase exhibited robust performance and no detectible non-template amplification, either with or without a 24 hour 25\u00b0C pre-incubation, while the ABI 1-Step Kit, featuring a non-WarmStart reverse transcriptase, exhibited significant non-template amplification. <\/span>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][vc_column_text]<\/p>\n<h2>Greater thermostability means greater versatility<\/h2>\n<ul>\n<li>Increased RT reaction temperature is ideal for difficult targets<\/li>\n<\/ul>\n<p>[\/vc_column_text][vc_column_text]<strong>The increased thermostability of WarmStart Luna Reverse Transcriptase improves performance at increased reaction temperatures <\/strong>[\/vc_column_text][vc_single_image image=&#8221;8687&#8243; img_size=&#8221;full&#8221; onclick=&#8221;img_link_large&#8221;][vc_column_text]<span class=\"bildunterschrift\"> RT-qPCR experiments targeting human ribosomal protein L32 RNA were performed in triplicate over a 5-log range of input human (Jurkat) total RNA (5 pg \u2013 50 ng) using an initial 10 min RT step performed at 50\u00b0C \u2013 60\u00b0C, as indicated. <\/span><\/p>\n<p><span class=\"bildunterschrift\"> A. Luna WarmStart Reverse Transcriptase (recommended incubation temperature: 55\u00b0C) exhibited rapid C<sub>q<\/sub> values (bar graph) and robust RT-qPCR performance (amplification plots) at each temperature, indicating that efficient reverse transcription was not perturbed by reaction temperature alterations. <\/span><\/p>\n<p><span class=\"bildunterschrift\"> B. In contrast, a commercially available MMLV (recommended incubation temperature: 50\u00b0C) exhibited delayed (increased) C<sub>q<\/sub> values, poorer performance, and loss of low-input detection at elevated temperatures, consistent with loss of RT activity. <\/span>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<span style=\"font-size: 10px;\">One or more of these products are covered by patents, trademarks and\/or copyrights owned or controlled by New England Biolabs, Inc. For more information, please email us at <a href=\"mailto:gbd@neb.com\">gbd@neb.com<\/a>. The use of these products may require you to obtain additional third party intellectual property rights for certain applications.<\/span><\/p>\n<p><span style=\"font-size: 10px;\">SYBR<sup>\u00ae<\/sup> is a registered trademark of Molecular Probes, Inc.<br \/>\nARRAYSCRIPT\u2122 is a trademark of Thermo Fisher Scientific,Inc.<\/span>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<\/p>\n<div class=\"wpb_text_column wpb_content_element \">\n<div class=\"wpb_wrapper\">\n<h2>Available products<\/h2>\n<table style=\"width: 683px; height: 1161px;\" border=\"0\" cellpadding=\"5\">\n<tbody>\n<tr style=\"background-color: #f3f3f3; font-size: 16px;\">\n<th style=\"border-bottom: solid #eb7e2d;\" align=\"left\" width=\"300\" height=\"40px\">PRODUCT<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"88\">REF.<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"100\">SIZE<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" colspan=\"1\">PRICE<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" colspan=\"1\">MORE INFO<\/th>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"1\"><span style=\"color: #f58025;\"><strong>NEW <\/strong><\/span>LunaScript\u00ae RT Master Mix Kit (Primer-free)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E3025S\/L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">25\/100 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/neb-online.fr\/en\/art\/E3025@\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter wp-image-4375 size-full\" src=\"https:\/\/www.neb-online.fr\/\/\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"In den Shop wechseln\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/international.neb.com\/products\/e3025-lunascript-rt-master-mix-kit-primer-free#Product%20Information\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter wp-image-4375 size-full\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Information Produit\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"1\"><span style=\"color: #f58025;\"><strong>NEW <\/strong><\/span>Luna SARS-CoV-2 RT-qPCR Multiplex Assay<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E3019S\/L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">96\/480 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/neb-online.fr\/en\/art\/E3019@\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter wp-image-4375 size-full\" src=\"https:\/\/www.neb-online.fr\/\/\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"In den Shop wechseln\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/international.neb.com\/products\/e3019-luna-sars-cov-2-rt-qpcr-multiplex-assay-kit#Product%20Information\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter wp-image-4375 size-full\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Information Produit\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"1\"><span style=\"color: #f58025;\"><strong>NEW <\/strong><\/span>Luna Probe One-Step RT-qPCR 4x Mix with UDG<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M3019S\/L\/X\/E<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">200\/500\/1000\/2000 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/neb-online.fr\/en\/art\/M3019@\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter wp-image-4375 size-full\" src=\"https:\/\/www.neb-online.fr\/\/\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"In den Shop wechseln\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/international.neb.com\/products\/m3019-luna-probe-one-step-rt-qpcr-4x-mix-with-udg#Product%20Information\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter wp-image-4375 size-full\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Information Produit\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"1\">Luna Cell Ready One-Step RT-qPCR Kit<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E3030S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/neb-online.fr\/en\/art\/E3030S\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter wp-image-4375 size-full\" src=\"https:\/\/www.neb-online.fr\/\/\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"In den Shop wechseln\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/products\/e3030-luna-cell-ready-one-step-rt-qpcr-kit#Product%20Information\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter wp-image-4375 size-full\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Information Produit\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"1\">Luna Cell Ready Probe One-Step RT-qPCR Kit<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E3031S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/neb-online.fr\/en\/art\/E3031\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter wp-image-4375 size-full\" src=\"https:\/\/www.neb-online.fr\/\/\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"Boutique en ligne\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/products\/e3031-luna-cell-ready-probe-one-step-rt-qpcr-kit#Product%20Information\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter wp-image-4375 size-full\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Information Produit\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"1\">Luna Cell Ready Lysis Module<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E3032S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/neb-online.fr\/en\/art\/E3032S\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter wp-image-4375 size-full\" src=\"https:\/\/www.neb-online.fr\/\/\/wp-content\/uploads\/2015\/05\/shop_icon1.gif\" alt=\"Boutique en ligne\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/products\/e3032-luna-cell-ready-lysis-module#Product%20Information\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter wp-image-4375 size-full\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Information Produit\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"4\">Luna Universal qPCR Master Mix<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M3003S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">200 rxns (2x1ml)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/neb-online.fr\/en\/art\/M3003S\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4375 size-full aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"http:\/\/international.neb.com\/products\/m3003-luna-universal-qpcr-master-mix\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4375 size-full aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M3003L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">500 rxns (5x1ml)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/neb-online.fr\/en\/art\/M3003L\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4375 size-full aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"http:\/\/international.neb.com\/products\/m3003-luna-universal-qpcr-master-mix\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4375 size-full aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M3003X<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">1000 rxns (10x1ml)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/neb-online.fr\/en\/art\/M3003X\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4375 size-full aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"http:\/\/international.neb.com\/products\/m3003-luna-universal-qpcr-master-mix\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4375 size-full aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M3003E<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">2500 rxns (1x25ml)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/neb-online.fr\/en\/art\/M3003E\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4375 size-full aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"http:\/\/international.neb.com\/products\/m3003-luna-universal-qpcr-master-mix\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4375 size-full aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"4\">Luna Universal Probe qPCR Master Mix<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M3004S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">200 rxns (2x1ml)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/neb-online.fr\/en\/art\/M3004S\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4375 size-full aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"http:\/\/international.neb.com\/products\/m3004-luna-universal-probe-qpcr-master-mix\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4375 size-full aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M3004L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">500 rxns (5x1ml)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/neb-online.fr\/en\/art\/M3004L\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4375 size-full aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"http:\/\/international.neb.com\/products\/m3004-luna-universal-probe-qpcr-master-mix\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4375 size-full aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M3004X<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">1000 rxns (10x1ml)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/neb-online.fr\/en\/art\/M3004X\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4375 size-full aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"http:\/\/international.neb.com\/products\/m3004-luna-universal-probe-qpcr-master-mix\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4375 size-full aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M3004E<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">2500 rxns (1x25ml)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/neb-online.fr\/en\/art\/M3004E\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4375 size-full aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"http:\/\/international.neb.com\/products\/m3004-luna-universal-probe-qpcr-master-mix\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4375 size-full aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"1\">Luna Universal One-Step RT-qPCR Kit<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E3005S\/L\/X\/E<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">200\/500\/1000\/2500 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/neb-online.fr\/en\/art\/E3005S\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4375 size-full aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"http:\/\/international.neb.com\/products\/e3005-luna-universal-one-step-rt-qpcr-kit\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4375 size-full aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"1\">Luna Universal Probe One-Step RT-qPCR Kit<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E3006S\/L\/X\/E<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">200\/500\/1000\/2500 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/neb-online.fr\/en\/art\/E3006S\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4375 size-full aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"http:\/\/international.neb.com\/products\/e3006-luna-universal-probe-one-step-rt-qpcr-kit\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4375 size-full aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"1\">LunaScript RT SuperMix Kit<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E3010S\/L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">25\/100 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/neb-online.fr\/en\/art\/E3010S\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4375 size-full aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/international.neb.com\/products\/e3010-luna-script-rt-supermix-kit#Product%20Information\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4375 size-full aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<div class=\"wpb_column vc_column_container vc_col-sm-12\">\n<div class=\"vc_column-inner \">\n<div class=\"wpb_wrapper\">\n<div class=\"wpb_text_column wpb_content_element \">\n<div class=\"wpb_wrapper\">\n<div class=\"wpb_column vc_column_container vc_col-sm-12\">\n<div class=\"vc_column-inner \">\n<div class=\"wpb_wrapper\">\n<div class=\"wpb_text_column wpb_content_element \">\n<div class=\"wpb_wrapper\"><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<p>[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row][vc_column][vc_column_text]The Luna RT-qPCR kits contain a novel, in silico-designed reverse transcriptase (RT) engineered for improved performance. Both the Luna WarmStart Reverse Transcriptase and Hot Start Taq DNA Polymerase, included in these kits, utilize a temperature-sensitive, reversible aptamer, which inhibits activity below 45\u00b0C. This enables room temperature reaction setup and prevents undesired non-specific activity. Furthermore, the&#8230;  <a class=\"excerpt-read-more\" href=\"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/luna-universal-qpcr-rt-qpcr\/luna-universal-qpcr-rt-qpcr-optimize-rt-qpcr-with-unique-warmstart-technology\/\" title=\"Read Luna\u00ae \u2013 Optimize RT-qPCR with unique WarmStart\u00ae technology\">Read more &raquo;<\/a><\/p>\n","protected":false},"author":15,"featured_media":8664,"parent":8731,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-sidebar-pcr-dna-amplification.php","meta":{"footnotes":""},"class_list":["post-8750","page","type-page","status-publish","has-post-thumbnail","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.0 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Luna\u00ae \u2013 Optimize RT-qPCR with unique WarmStart\u00ae technology - New England Biolabs France<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.neb-online.fr\/en\/pcr-and-dna-amplification\/luna-universal-qpcr-rt-qpcr\/luna-universal-qpcr-rt-qpcr-optimize-rt-qpcr-with-unique-warmstart-technology\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Luna\u00ae \u2013 Optimize RT-qPCR with unique WarmStart\u00ae technology - New England Biolabs France\" \/>\n<meta property=\"og:description\" content=\"[vc_row][vc_column][vc_column_text]The Luna RT-qPCR kits contain a novel, in silico-designed reverse transcriptase (RT) engineered for improved performance. Both the Luna WarmStart Reverse Transcriptase and Hot Start Taq DNA Polymerase, included in these kits, utilize a temperature-sensitive, reversible aptamer, which inhibits activity below 45\u00b0C. This enables room temperature reaction setup and prevents undesired non-specific activity. Furthermore, the... 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