{"id":9564,"date":"2017-08-22T17:02:28","date_gmt":"2017-08-22T15:02:28","guid":{"rendered":"http:\/\/www.neb-online.fr\/en\/?page_id=9564"},"modified":"2020-04-16T21:27:38","modified_gmt":"2020-04-16T19:27:38","slug":"ultraiirna","status":"publish","type":"page","link":"https:\/\/www.neb-online.fr\/en\/next-generation-sequencing\/ultraiirna\/","title":{"rendered":"NEBNext Ultra II RNA Library Prep Kit and Modules"},"content":{"rendered":"

[vc_row][vc_column][vc_column_text el_class=”.chapo”]Do you need increased sensitivity and specificity from your RNA-seq experiments? Do you have ever-decreasing amounts of input RNA?[\/vc_column_text][vc_column_text el_class=”.texte_courant”]To address these challenges, our next generation of RNA library prep kits have been reformulated at each step, resulting in several fold higher yields of high quality libraries, enabling use of lower input amounts and fewer PCR cycles.[\/vc_column_text][vc_column_text el_class=”.titre_paragraphe”]Why consider the NEBNext Ultra II (Directional) RNA Library Prep Kit for Illumina?[\/vc_column_text][vc_column_text el_class=”.texte_courant”]Our new NEBNext Ultra II RNA kits have streamlined, automatable workflows and are available for directional (strand-specific, using the \u00ab\u00a0dUTP method\u00a0\u00bb) and non-directional library prep, with the option of SPRIselect\u00ae<\/sup> beads for size-selection and clean-up steps.[\/vc_column_text][vc_accordion active_tab=”false” collapsible=”yes” el_class=”mauricio-accordion”][vc_accordion_tab title=”Advantages of the NEBNext ultra II RNA kits:”][vc_row_inner][vc_column_inner][vc_column_text el_class=”.liste”]Get more of what you need, with the highest library yields<\/p>\n

Generate high quality libraries even when you have only limited amounts of input RNA:[\/vc_column_text][vc_column_text el_class=”.sous-liste” css=”.vc_custom_1503406952799{margin-top: -14px !important;margin-bottom: 21px !important;}”]10 ng \u2013 1 \u00b5g Total RNA (polyA mRNA workflow)<\/p>\n

5 ng \u2013 1 \u00b5g Total RNA (rRNA depletion workflow)[\/vc_column_text][vc_column_text el_class=”.liste”]Minimize bias, with fewer PCR cycles required<\/p>\n

Maximize the flexibility to order reagents for your specific workflow needs:[\/vc_column_text][vc_column_text el_class=”.sous-liste” css=”.vc_custom_1503407332396{margin-top: -14px !important;margin-bottom: 21px !important;}”]Directional (strand-specific, using the \u201cdUTP method\u201d) and non-directional workflow options<\/p>\n

rRNA Depletion and poly(A) mRNA Isolation reagents<\/p>\n

Adaptors and primers for multiplexing (12- and 96-index) formats[\/vc_column_text][vc_column_text el_class=”.liste”]Enjoy the reliability of the gold standard SPRIselect size selection and clean-up beads, supplied in just the amounts you need<\/p>\n

Optimize your time with streamlined workflows, reduced hands-on time, and automation compatibility<\/p>\n

Rely on robust performance, even with low quality RNA[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_accordion_tab][\/vc_accordion][vc_row_inner][vc_column_inner width=”1\/2″][vc_single_image image=”9026″ img_size=”full”][\/vc_column_inner][vc_column_inner width=”1\/2″][vc_column_text el_class=”.texte_courant p”]Poly(A)-containing mRNA was isolated from 10 ng, 100 ng and 1 \u00b5g of Universal Human Reference RNA (Agilent #740000) and libraries were made using the NEBNext Ultra II Directional RNA kit, Kapa Stranded mRNA-Seq kit, Kapa mRNA HyperPrep kit and Illumina TruSeq Stranded mRNA Kit. The input RNA amount and number of PCR cycles are indicated. Library yields from an average of three replicates are shown. Error bars indicate standard deviation. Library yields were assessed using the Agilent\u00ae Bioanalyzer\u00ae.<\/i>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_separator color=”custom” accent_color=”#eb7e2d”][vc_row_inner][vc_column_inner width=”1\/2″][vc_column_text el_class=”.texte_courant”]NEBNext Ultra II Products are easily automatable and have been implemented on diverse platforms.[\/vc_column_text][\/vc_column_inner][vc_column_inner width=”1\/2″]NEBNext Automation<\/a>[\/vc_column_inner][\/vc_row_inner][vc_column_text el_class=”.titre_paragraphe”]Product Table[\/vc_column_text][vc_column_text el_class=”.texte_courant”]<\/p>\n\n\n\n\n\n\n\n\n
PRODUCT<\/th>\nProd.Nr.:<\/th>\nSIZE<\/th>\nPRICE<\/th>\nMORE INFO<\/th>\n<\/tr>\n
NEW<\/strong><\/span> NEBNext Magnetic Separation Rack<\/td>\nS1515S<\/td>\n1 rack<\/td>\n\"NEB<\/a><\/td>\n\"NEB<\/a><\/td>\n<\/tr>\n
NEBNext Ultra II Directional RNA Library Prep Kit<\/td>\nE7760S\/L<\/td>\n24\/96 rxns<\/td>\n\"NEB<\/a><\/td>\n\"NEB<\/a><\/td>\n<\/tr>\n
NEBNext Ultra II Directional RNA Library Prepwith Sample Purification Beads<\/td>\nE7765S\/L<\/td>\n24\/96 rxns<\/td>\n\"NEB<\/a><\/td>\n\"NEB<\/a><\/td>\n<\/tr>\n
NEBNext Ultra II RNA Library Prep Kit<\/td>\nE7770S\/L<\/td>\n24\/96 rxns<\/td>\n\"NEB<\/a><\/td>\n\"NEB<\/a><\/td>\n<\/tr>\n
NEBNext Ultra II RNA Library Prep Kit
\nwith Sample Purification Beads<\/td>\n		E7775S\/L<\/td>\n24\/96 rxns<\/td>\n\"NEB<\/a><\/td>\n\"NEB<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

[\/vc_column_text][vc_column_text el_class=”.texte_courant”]This content is covered by patents, trademarks and\/or copyrights owned or controlled by New England Biolabs, Inc. For more information, please email us at gbd@neb.com<\/a>. The use of these products may require you to obtain additional third party intellectual property rights for certain applications.<\/p>\n

KAPA\u00ae is a registered trademark of Kapa Biosystems<\/p>\n

ILLUMINA\u00ae and TRUSEQ\u00ae are registered trademarks of Illumina, Inc.<\/p>\n

AGILENT\u00ae and BIOANALYZER\u00ae are registered trademarks of Agilent Technologies<\/p>\n

SPRISELECT\u00ae is a registered trademark of Beckman Coulter, Inc.[\/vc_column_text][vc_separator color=”custom” accent_color=”#eb7e2d”][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"

[vc_row][vc_column][vc_column_text el_class=”.chapo”]Do you need increased sensitivity and specificity from your RNA-seq experiments? Do you have ever-decreasing amounts of input RNA?[\/vc_column_text][vc_column_text el_class=”.texte_courant”]To address these challenges, our next generation of RNA library prep kits have been reformulated at each step, resulting in several fold higher yields of high quality libraries, enabling use of lower input amounts and… Read more »<\/a><\/p>\n","protected":false},"author":15,"featured_media":9029,"parent":2170,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-sidebar-next-generation-sequencing.php","meta":{"footnotes":""},"class_list":["post-9564","page","type-page","status-publish","has-post-thumbnail","hentry"],"yoast_head":"\nNEBNext Ultra II RNA Library Prep Kit and Modules - New England Biolabs France<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.neb-online.fr\/en\/next-generation-sequencing\/ultraiirna\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"NEBNext Ultra II RNA Library Prep Kit and Modules - New England Biolabs France\" \/>\n<meta property=\"og:description\" content=\"[vc_row][vc_column][vc_column_text el_class=”.chapo”]Do you need increased sensitivity and specificity from your RNA-seq experiments? 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