{"id":9983,"date":"2017-11-30T15:29:11","date_gmt":"2017-11-30T14:29:11","guid":{"rendered":"http:\/\/www.neb-online.fr\/en\/neb-en\/monarch-nucleic-acid-purification-kits\/kit-monarch-purification-darn\/"},"modified":"2022-07-29T10:03:30","modified_gmt":"2022-07-29T08:03:30","slug":"kit-monarch-purification-darn","status":"publish","type":"page","link":"https:\/\/www.neb-online.fr\/en\/monarch-nucleic-acid-purification-kits\/kit-monarch-purification-darn\/","title":{"rendered":"Monarch Total RNA Miniprep Kit"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row css=&#8221;.vc_custom_1511345090011{margin-top: 30px !important;}&#8221;][vc_column][vc_column_text]<span style=\"font-size: 18px;\"><strong>RNA purification made easy:<\/strong><\/span><br \/>\n<span style=\"font-size: 26px;\">The &#8220;universal&#8221; <span style=\"color: #dd7500;\">Monarch<sup>\u00ae<\/sup> RNA Purification Kit<\/span> for quick and easy purification of total RNA from cells, tissue, blood, bacteria, plants&#8230;<\/span>[\/vc_column_text][\/vc_column][\/vc_row][vc_row css=&#8221;.vc_custom_1511421982993{margin-right: -1px !important;margin-bottom: 20px !important;}&#8221;][vc_column width=&#8221;1\/2&#8243;][vc_column_text]The Monarch Total RNA Miniprep Kit is a comprehensive solution for sample preservation, cell lysis, gDNA removal, and purification of total RNA from a wide variety of biological samples, including cultured cells, blood, and mammalian tissues.<\/p>\n<p>Additionally, tough-to-lyse samples, such as bacteria, yeast, and plant, can be processed with additional steps that enhance lysis. Cleanup of enzymatic reactions or purification of RNA from TRIzol<sup>\u00ae<\/sup> -extracted samples is also possible using this kit. Purified RNA has high quality metrics, including A<sub>260\/280<\/sub> and A<sub>260\/230<\/sub> ratios &gt;1.8, high RIN scores, and minimal residual gDNA. Captured RNA ranges in size from full-length rRNAs down to intact miRNAs.<\/p>\n<p>Additionally, differential binding conditions allow selective capture or exclusion of the sub-200 nucleotide RNA pool that includes miRNA, 5S rRNA, and tRNA. Purified RNA is suitable for downstream applications, such as RT-qPCR, cDNA synthesis, RNA-seq, Northern blot analysis, etc.[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/2&#8243; css=&#8221;.vc_custom_1511345071485{margin-right: -1px !important;border-top-width: 2px !important;border-bottom-width: 2px !important;background-color: #f4f1e7 !important;border-top-color: #eb7e2d !important;border-top-style: solid !important;border-bottom-color: #eb7e2d !important;border-bottom-style: solid !important;}&#8221;][vc_column_text]<span style=\"font-size: 18px;\"><strong>Monarch<sup>\u00ae<\/sup> Total RNA Miniprep Kit \u2013<br \/>\nYour Advantages:<\/strong><\/span><\/p>\n<ul>\n<li>For use with blood, cells and tissues<\/li>\n<li>Also works with tough to lyse samples<br \/>\n(bacteria, yeast, plant)<\/li>\n<li>Effectively purifies total RNA including<br \/>\nsmall RNA &lt;200 nt<\/li>\n<li>Efficient genomic DNA removal<br \/>\n(column and DNase I-based)<\/li>\n<li>Contains Proteinase K for processing of<br \/>\ntissues and blood samples<\/li>\n<li>Includes RNA Protection Reagent for<br \/>\nsample preservation<\/li>\n<li>Excellent value<\/li>\n<li>Kit components available separately<\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][vc_row_inner][vc_column_inner width=&#8221;1\/4&#8243;][vc_single_image image=&#8221;18416&#8243; img_size=&#8221;full&#8221;][\/vc_column_inner][vc_column_inner width=&#8221;3\/4&#8243;][vc_column_text]<\/p>\n<h2>Just like our <a href=\"https:\/\/www.neb-online.fr\/en\/monarch-nucleic-acid-purification-kits\/monarch-nucleic-acid-purification-kits-copy\/\">Monarch DNA purification kits<\/a> this Monarch Total RNA Miniprep Kit has been optimised for maximum performance with minimal environmental impact and all components, columns and buffers are available separately.<\/h2>\n<p>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_separator][\/vc_column][\/vc_row][vc_row][vc_column][vc_row_inner][vc_column_inner width=&#8221;1\/2&#8243;][vc_single_image image=&#8221;18418&#8243; img_size=&#8221;full&#8221; add_caption=&#8221;yes&#8221; alignment=&#8221;center&#8221; onclick=&#8221;link_image&#8221;][\/vc_column_inner][vc_column_inner width=&#8221;1\/2&#8243;][vc_column_text]<span class=\"bildunterschrift\">Total RNA from a broad array of sample types was purified using the Monarch Total RNA Miniprep Kit (NEB #T2010). Aliquots were run on an Agilent\u00ae Bioanalyzer\u00ae 2100 using the Nano 6000 RNA chip (S. cerevisiae RNA was run using a plant Nano assay). RIN values and O.D. ratios confirm the overall integrity and purity of the RNA. To demonstrate compatibility with downstream applications, samples were subsequently used for RT-PCR (+\/\u2013 RT) (A) for detection of 4 different RNA species using Protoscript\u00ae II Reverse Transcriptase (NEB #M0368)\/LongAmp\u00ae Taq DNA Polymerase (NEB #M0323), NGS library prep (B) using NEBNext\u00ae Ultra\u2122 II RNA Library Prep Kit (NEB #E7760) and RT-qPCR (C) using Luna\u00ae One-Step RT-qPCR Reagents (NEB #E3005).<\/span>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_separator][vc_row_inner][vc_column_inner width=&#8221;1\/2&#8243;][vc_single_image image=&#8221;18417&#8243; img_size=&#8221;full&#8221; alignment=&#8221;center&#8221; onclick=&#8221;link_image&#8221;][\/vc_column_inner][vc_column_inner width=&#8221;1\/2&#8243;][vc_column_text]<span class=\"bildunterschrift\">Transcript levels in Universal Human Reference RNA (UHRR, Agilent) are compared before and after re-purification using either Qiagen RNeasy\u00ae or the Monarch Total RNA Miniprep Kit. Strong correlation with untreated UHRR is observed for both methods (Pearson R &gt; 0.99 for both samples). All samples display consistent end-to-end coverage of transcripts indicating an absence of detectable degradation during purification. Poly-A selected RNA was selected from 100 ng of untreated, Qiagen and Monarch samples using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490). RNA-seq libraries were then prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina\u00ae (NEB #E7760) before sequencing on a Miseq\u00ae instrument (2 x150). 1.6M reads were randomly sampled from each library and adapter trimmed (Seqprep v1.1). Levels of all Gencode v26 transcripts were assessed using Salmon (0.4) and plotted above (panel A). Average 5&#8242;-3&#8242; Coverage of Gencode v26 transcripts (assessed by Picard&#8217;s CollectRnaSeqMetrics 1.56 after mapping to the GRCh38 reference genome with Hisat v2.0.3 and marking duplicates with Picard&#8217;s MarkDuplicates 1.56 ) is shown below (panel B).<\/span>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][vc_column_text]<\/p>\n<h2>Available products<\/h2>\n<table style=\"width: 760px;\" border=\"0\" cellpadding=\"5\">\n<tbody>\n<tr style=\"background-color: #f3b281;\">\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"350\" height=\"40px\">PRODUCT<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"80\">REF.<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"140\">COND.<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" colspan=\"1\" width=\"190\">MORE INFOS<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" colspan=\"1\" width=\"190\">PRICE<\/th>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Monarch\u2122 Total RNA Miniprep Kit<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">T2010S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">50 preps<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"26px\"><a href=\"http:\/\/international.neb.com\/products\/t2010-monarch-total-rna-miniprep-kit#Product Information\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter\" src=\"http:\/\/www.neb-online.fr\/enews\/enews_NEB_button_info.png\" alt=\"NEB Shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"26px\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/T2010s\"><img decoding=\"async\" class=\"aligncenter\" src=\"http:\/\/www.neb-online.fr\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"1\">Monarch<sup>\u2122<\/sup> Microfuge Tube EcoRack<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">T5020S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">2 racks<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/international.neb.com\/products\/t5020-monarch-microfuge-tube-ecorack-2-racks#Product%20Information\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/T5020s\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td colspan=\"6\" height=\"20px\"><\/td>\n<\/tr>\n<tr>\n<td style=\"background-color: #d2d2d2;\" colspan=\"6\">Colonnes disponibles s\u00e9par\u00e9ment<\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Monarch\u2122 RNA Purification Columns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">T2007L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 preps<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"http:\/\/international.neb.com\/products\/t2007-monarch-rna-purification-columns#Product%20Information\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter\" src=\"http:\/\/www.neb-online.fr\/enews\/enews_NEB_button_info.png\" alt=\"NEB Shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/T2007l\"><img decoding=\"async\" class=\"aligncenter\" src=\"http:\/\/www.neb-online.fr\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Monarch\u2122 gDNA Removal Columns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">T2017L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 preps<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"http:\/\/international.neb.com\/products\/t2017-monarch-gdna-removal-columns#Product%20Information\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter\" src=\"http:\/\/www.neb-online.fr\/enews\/enews_NEB_button_info.png\" alt=\"NEB Shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/T2017l\"><img decoding=\"async\" class=\"aligncenter\" src=\"http:\/\/www.neb-online.fr\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Monarch\u2122 Collection Tubes II<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">T2018L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 preps<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"http:\/\/international.neb.com\/products\/t2018-monarch-collection-tubes-ii#Product%20Information\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter\" src=\"http:\/\/www.neb-online.fr\/enews\/enews_NEB_button_info.png\" alt=\"NEB Shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/T2018l\"><img decoding=\"async\" class=\"aligncenter\" src=\"http:\/\/www.neb-online.fr\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td colspan=\"6\" height=\"20px\"><\/td>\n<\/tr>\n<tr>\n<td style=\"background-color: #d2d2d2;\" colspan=\"6\">Tampons &amp; r\u00e9actifs disponibles s\u00e9par\u00e9ment<\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Monarch\u2122 DNA\/RNA Protection Reagent<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">T2011L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">56 ml<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"http:\/\/international.neb.com\/products\/t2011-monarch-dna-rna-protection-reagent#Product%20Information\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter\" style=\"float: center;\" src=\"http:\/\/www.neb-online.fr\/enews\/enews_NEB_button_info.png\" alt=\"NEB Shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/T2011l\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter\" style=\"float: center;\" src=\"http:\/\/www.neb-online.fr\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Monarch\u2122 RNA Lysis Buffer<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">T2012L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 ml<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"http:\/\/international.neb.com\/products\/t2012-monarch-rna-lysis-buffer#Product%20Information\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter\" style=\"float: center;\" src=\"http:\/\/www.neb-online.fr\/enews\/enews_NEB_button_info.png\" alt=\"NEB Shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/T2012l\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter\" style=\"float: center;\" src=\"http:\/\/www.neb-online.fr\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Monarch\u2122 Total RNA Miniprep Enzyme Pack<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">T2019L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">1 pack<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"http:\/\/international.neb.com\/products\/t2019-monarch-total-rna-miniprep-enzyme-pack#Product%20Information\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter\" style=\"float: center;\" src=\"http:\/\/www.neb-online.fr\/enews\/enews_NEB_button_info.png\" alt=\"NEB Shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/T2019l\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter\" style=\"float: center;\" src=\"http:\/\/www.neb-online.fr\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Monarch\u2122 RNA Priming Buffer<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">T2013L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">56 ml<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"http:\/\/international.neb.com\/products\/t2013-monarch-rna-priming-buffer#Product%20Information\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter\" style=\"float: center;\" src=\"http:\/\/www.neb-online.fr\/enews\/enews_NEB_button_info.png\" alt=\"NEB Shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/T2013@\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter\" style=\"float: center;\" src=\"http:\/\/www.neb-online.fr\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Monarch\u2122 RNA Wash Buffer<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">T2014L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">50 ml<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"http:\/\/international.neb.com\/products\/t2014-monarch-rna-wash-buffer#Product%20Information\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter\" style=\"float: center;\" src=\"http:\/\/www.neb-online.fr\/enews\/enews_NEB_button_info.png\" alt=\"NEB Shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/T2014l\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter\" style=\"float: center;\" src=\"http:\/\/www.neb-online.fr\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Nuclease-free Water<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">B1500S\/L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">25 ml \/ 4&#215;25 ml<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"http:\/\/international.neb.com\/products\/b1500-nuclease-free-water#Product%20Information\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter\" style=\"float: center;\" src=\"http:\/\/www.neb-online.fr\/enews\/enews_NEB_button_info.png\" alt=\"NEB Shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/shop.neb-online.fr\/en\/art\/B1500@\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter\" style=\"float: center;\" src=\"http:\/\/www.neb-online.fr\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"25\" height=\"25\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row css=&#8221;.vc_custom_1511349251015{margin-top: 20px !important;margin-right: -1px !important;margin-bottom: 50px !important;margin-left: -1px !important;border-top-width: 2px !important;border-bottom-width: 2px !important;background-color: #f4f1e7 !important;border-top-color: #eb7e2d !important;border-top-style: solid !important;border-bottom-color: #eb7e2d !important;border-bottom-style: solid !important;}&#8221;][vc_column][vc_column_text]<\/p>\n<h2>Experience the Monarch<sup>\u00ae <\/sup>advantages and try our free Total RNA Miniprep sample!<\/h2>\n<p>[\/vc_column_text]<a class=\"btn btn-standard form-btn\" target=\"_blank\" href=\"https:\/\/newenglandbiolabs.tfaforms.net\/338514\" style=\"margin-left:auto;margin-right:auto;\">Request your sample here!<\/a>[\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row css=&#8221;.vc_custom_1511345090011{margin-top: 30px !important;}&#8221;][vc_column][vc_column_text]RNA purification made easy: The &#8220;universal&#8221; Monarch\u00ae RNA Purification Kit for quick and easy purification of total RNA from cells, tissue, blood, bacteria, plants&#8230;[\/vc_column_text][\/vc_column][\/vc_row][vc_row css=&#8221;.vc_custom_1511421982993{margin-right: -1px !important;margin-bottom: 20px !important;}&#8221;][vc_column width=&#8221;1\/2&#8243;][vc_column_text]The Monarch Total RNA Miniprep Kit is a comprehensive solution for sample preservation, cell lysis, gDNA removal, and purification of total RNA from&#8230;  <a class=\"excerpt-read-more\" href=\"https:\/\/www.neb-online.fr\/en\/monarch-nucleic-acid-purification-kits\/kit-monarch-purification-darn\/\" title=\"Read Monarch Total RNA Miniprep Kit\">Read more &raquo;<\/a><\/p>\n","protected":false},"author":15,"featured_media":18414,"parent":7186,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-sidebar-nucleic-acid-purification.php","meta":{"footnotes":""},"class_list":["post-9983","page","type-page","status-publish","has-post-thumbnail","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.0 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Monarch Total RNA Miniprep Kit - New England Biolabs France<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.neb-online.fr\/en\/monarch-nucleic-acid-purification-kits\/kit-monarch-purification-darn\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Monarch Total RNA Miniprep Kit - New England Biolabs France\" \/>\n<meta property=\"og:description\" content=\"[vc_row css=&#8221;.vc_custom_1511345090011{margin-top: 30px !important;}&#8221;][vc_column][vc_column_text]RNA purification made easy: The &#8220;universal&#8221; Monarch\u00ae RNA Purification Kit for quick and easy purification of total RNA from cells, tissue, blood, bacteria, plants&#8230;[\/vc_column_text][\/vc_column][\/vc_row][vc_row css=&#8221;.vc_custom_1511421982993{margin-right: -1px !important;margin-bottom: 20px !important;}&#8221;][vc_column width=&#8221;1\/2&#8243;][vc_column_text]The Monarch Total RNA Miniprep Kit is a comprehensive solution for sample preservation, cell lysis, gDNA removal, and purification of total RNA from... Read more &raquo;\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.neb-online.fr\/en\/monarch-nucleic-acid-purification-kits\/kit-monarch-purification-darn\/\" \/>\n<meta property=\"og:site_name\" content=\"New England Biolabs France\" \/>\n<meta property=\"article:modified_time\" content=\"2022-07-29T08:03:30+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.neb-online.fr\/wp-content\/uploads\/banner-Monarch_UGAP_760x220-scaled.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"2560\" \/>\n\t<meta property=\"og:image:height\" content=\"741\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"9 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.neb-online.fr\/en\/monarch-nucleic-acid-purification-kits\/kit-monarch-purification-darn\/\",\"url\":\"https:\/\/www.neb-online.fr\/en\/monarch-nucleic-acid-purification-kits\/kit-monarch-purification-darn\/\",\"name\":\"Monarch Total RNA Miniprep Kit - New England Biolabs France\",\"isPartOf\":{\"@id\":\"https:\/\/www.neb-online.fr\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\/\/www.neb-online.fr\/en\/monarch-nucleic-acid-purification-kits\/kit-monarch-purification-darn\/#primaryimage\"},\"image\":{\"@id\":\"https:\/\/www.neb-online.fr\/en\/monarch-nucleic-acid-purification-kits\/kit-monarch-purification-darn\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/www.neb-online.fr\/wp-content\/uploads\/banner-Monarch_UGAP_760x220-scaled.jpg\",\"datePublished\":\"2017-11-30T14:29:11+00:00\",\"dateModified\":\"2022-07-29T08:03:30+00:00\",\"breadcrumb\":{\"@id\":\"https:\/\/www.neb-online.fr\/en\/monarch-nucleic-acid-purification-kits\/kit-monarch-purification-darn\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/www.neb-online.fr\/en\/monarch-nucleic-acid-purification-kits\/kit-monarch-purification-darn\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/www.neb-online.fr\/en\/monarch-nucleic-acid-purification-kits\/kit-monarch-purification-darn\/#primaryimage\",\"url\":\"https:\/\/www.neb-online.fr\/wp-content\/uploads\/banner-Monarch_UGAP_760x220-scaled.jpg\",\"contentUrl\":\"https:\/\/www.neb-online.fr\/wp-content\/uploads\/banner-Monarch_UGAP_760x220-scaled.jpg\",\"width\":2560,\"height\":741},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/www.neb-online.fr\/en\/monarch-nucleic-acid-purification-kits\/kit-monarch-purification-darn\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Monarch Kits &#8211; Nucleic Acid Purification\",\"item\":\"https:\/\/www.neb-online.fr\/en\/monarch-nucleic-acid-purification-kits\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Monarch Total RNA Miniprep Kit\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/www.neb-online.fr\/#website\",\"url\":\"https:\/\/www.neb-online.fr\/\",\"name\":\"New England Biolabs France\",\"description\":\"be INSPIRED, drive DISCOVERY, stay GENUINE\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/www.neb-online.fr\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Monarch Total RNA Miniprep Kit - New England Biolabs France","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.neb-online.fr\/en\/monarch-nucleic-acid-purification-kits\/kit-monarch-purification-darn\/","og_locale":"en_US","og_type":"article","og_title":"Monarch Total RNA Miniprep Kit - New England Biolabs France","og_description":"[vc_row css=&#8221;.vc_custom_1511345090011{margin-top: 30px !important;}&#8221;][vc_column][vc_column_text]RNA purification made easy: The &#8220;universal&#8221; Monarch\u00ae RNA Purification Kit for quick and easy purification of total RNA from cells, tissue, blood, bacteria, plants&#8230;[\/vc_column_text][\/vc_column][\/vc_row][vc_row css=&#8221;.vc_custom_1511421982993{margin-right: -1px !important;margin-bottom: 20px !important;}&#8221;][vc_column width=&#8221;1\/2&#8243;][vc_column_text]The Monarch Total RNA Miniprep Kit is a comprehensive solution for sample preservation, cell lysis, gDNA removal, and purification of total RNA from... Read more &raquo;","og_url":"https:\/\/www.neb-online.fr\/en\/monarch-nucleic-acid-purification-kits\/kit-monarch-purification-darn\/","og_site_name":"New England Biolabs France","article_modified_time":"2022-07-29T08:03:30+00:00","og_image":[{"width":2560,"height":741,"url":"https:\/\/www.neb-online.fr\/wp-content\/uploads\/banner-Monarch_UGAP_760x220-scaled.jpg","type":"image\/jpeg"}],"twitter_misc":{"Est. reading time":"9 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/www.neb-online.fr\/en\/monarch-nucleic-acid-purification-kits\/kit-monarch-purification-darn\/","url":"https:\/\/www.neb-online.fr\/en\/monarch-nucleic-acid-purification-kits\/kit-monarch-purification-darn\/","name":"Monarch Total RNA Miniprep Kit - New England Biolabs France","isPartOf":{"@id":"https:\/\/www.neb-online.fr\/#website"},"primaryImageOfPage":{"@id":"https:\/\/www.neb-online.fr\/en\/monarch-nucleic-acid-purification-kits\/kit-monarch-purification-darn\/#primaryimage"},"image":{"@id":"https:\/\/www.neb-online.fr\/en\/monarch-nucleic-acid-purification-kits\/kit-monarch-purification-darn\/#primaryimage"},"thumbnailUrl":"https:\/\/www.neb-online.fr\/wp-content\/uploads\/banner-Monarch_UGAP_760x220-scaled.jpg","datePublished":"2017-11-30T14:29:11+00:00","dateModified":"2022-07-29T08:03:30+00:00","breadcrumb":{"@id":"https:\/\/www.neb-online.fr\/en\/monarch-nucleic-acid-purification-kits\/kit-monarch-purification-darn\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/www.neb-online.fr\/en\/monarch-nucleic-acid-purification-kits\/kit-monarch-purification-darn\/"]}]},{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/www.neb-online.fr\/en\/monarch-nucleic-acid-purification-kits\/kit-monarch-purification-darn\/#primaryimage","url":"https:\/\/www.neb-online.fr\/wp-content\/uploads\/banner-Monarch_UGAP_760x220-scaled.jpg","contentUrl":"https:\/\/www.neb-online.fr\/wp-content\/uploads\/banner-Monarch_UGAP_760x220-scaled.jpg","width":2560,"height":741},{"@type":"BreadcrumbList","@id":"https:\/\/www.neb-online.fr\/en\/monarch-nucleic-acid-purification-kits\/kit-monarch-purification-darn\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Monarch Kits &#8211; Nucleic Acid Purification","item":"https:\/\/www.neb-online.fr\/en\/monarch-nucleic-acid-purification-kits\/"},{"@type":"ListItem","position":2,"name":"Monarch Total RNA Miniprep Kit"}]},{"@type":"WebSite","@id":"https:\/\/www.neb-online.fr\/#website","url":"https:\/\/www.neb-online.fr\/","name":"New England Biolabs France","description":"be INSPIRED, drive DISCOVERY, stay GENUINE","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/www.neb-online.fr\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"}]}},"_links":{"self":[{"href":"https:\/\/www.neb-online.fr\/en\/wp-json\/wp\/v2\/pages\/9983","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.neb-online.fr\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.neb-online.fr\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.neb-online.fr\/en\/wp-json\/wp\/v2\/users\/15"}],"replies":[{"embeddable":true,"href":"https:\/\/www.neb-online.fr\/en\/wp-json\/wp\/v2\/comments?post=9983"}],"version-history":[{"count":4,"href":"https:\/\/www.neb-online.fr\/en\/wp-json\/wp\/v2\/pages\/9983\/revisions"}],"predecessor-version":[{"id":24033,"href":"https:\/\/www.neb-online.fr\/en\/wp-json\/wp\/v2\/pages\/9983\/revisions\/24033"}],"up":[{"embeddable":true,"href":"https:\/\/www.neb-online.fr\/en\/wp-json\/wp\/v2\/pages\/7186"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.neb-online.fr\/en\/wp-json\/wp\/v2\/media\/18414"}],"wp:attachment":[{"href":"https:\/\/www.neb-online.fr\/en\/wp-json\/wp\/v2\/media?parent=9983"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}