NOUVEAU : NEBNext® Direct Custom Ready Panel

Employing the unique NEBNext Direct hybridization-based enrichment method, NEBNext Direct Custom Ready Panels allow rapid customization of targeted gene panels for Illumina sequencing. Select from a list of genes for which baits have been carefully designed and optimized to give complete coverage of the full coding regions. High quality panels can be designed by you and rapidly delivered, from any combination of genes. NEBNext Direct Custom Ready Panels provide the content you want with the performance you need.

Click here for more information on the NEBNext Direct Target Enrichment technology

Avantages:
  • Choose from a single gene to hundreds of genes
  • Experience unmatched specificity and coverage uniformity
  • Eliminate synthesis and optimization steps for faster turnaround
  • Improve sensitivity with our Unique Molecule Index (UMI)
  • Generate results in one day with our automation-friendly workflow

NEBNext Direct Custom Ready Panels demonstrate optimum performance across a wide range of panel sizes

Key target enrichment metrics demonstrate consistent performance across a range of panel sizes. 100 ng of DNA was tested against panels of 1, 10, 25, 50 and 100 genes, and sequenced using Illumina paired-end 150 bp sequencing. Larger panels included all genes present in smaller panels.

Sensitivity in detection of variants across panel size and DNA input amount

24 HapMap samples were blended to create a range of variant allele frequencies (VAF) down to 2%. 25, 50, 100, 200 and 500 ng of this blended DNA was enriched using NEBNext Direct Custom Ready Panels of 1, 10, 25, 50, and 100 genes. Larger panels were inclusive of the genes in smaller panels. Resulting libraries were sequenced using 2 x 150 bp Illumina sequencing and variants were called using Mutect and Vardict variant calling algorithms.

NEBNext Direct Custom Ready Panels demonstrate retention of target behavior across panel sizes

IGV image of coverage profile for 4 BRAF exons included in panels of 1, 10, 25, 50 and 100 genes, demonstrate consistent target behavior with the addition of gene targets. 100 ng of DNA was used as input for NEBNext enrichment using the 5 panels, including the BRAF gene. Libraries were sequenced on an Illumina 2 x 150 basepair sequencing.

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Liste Produits

PRODUIT REF. # COND. PRIX
NOUVEAU : NEBNext Direct® Custom Ready Panels E6631S 8 rxns sur demande; dépendant des panels choisis Produktinformationen
E6631L 24 rxns
E6631X 96 rxns
NEBNext Direct® Cancer HotSpot Panel E7000S 8 rxns 1.450 € In den Shop wechseln Produktinformationen
E7000L 24 rxns 4.200 € In den Shop wechseln Produktinformationen
E7000X 96 rxns 15.800 € In den Shop wechseln Produktinformationen
NEBNext Direct® BRCA1/BRCA2 Panel E6627S 8 rxns 660 € In den Shop wechseln Produktinformationen
E6627L 24 rxns 1.900 € In den Shop wechseln Produktinformationen
E6627X 96 rxns 6.950 € In den Shop wechseln Produktinformationen

Au 02.01.2018

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