Cloning Strains
For cloning experiments choose from several high efficiency competent cell strains. These E. coli strains are T1 phage resistant and are endA deficient for high quality plasmid preparations. Additionally, all competent cells from NEB are free of animal products.
Yeast
NEB offers chemically competent Kluyveromyces lactis cells and variants of this strain that have been tailored for specific protein expression needs. These cells are suitable for transformation with any of our linearized pKLAC series expression vectors.
E. coli for Protein Expression
NEB offers several strains with varying levels of expression control, each with T1 phage resistance and extremely high transformation efficiencies.
Competent E. coli – Competitor Cross-Reference Tool
Use this tool to select another company’s competent cell product and find out which NEB strain is compatible. Choose either the product name or catalog number from the available selection, and this tool will identify the recommended NEB product and its advantages. A link to the product page where you can also order the product is provided.
Competitor Cross-Reference Tool
Properties Table
| Strain Properties |
NEB# | Transform Efficiency (cfu/µg)(1) |
Strain | T1 Phage Resistant |
Library Construc -tion(2) |
Cloning/ Sub- cloning |
Blue/ White Screening |
laclq | lysY | Endo- nucleaseI Deficient(3) |
Protease(4) Deficient |
F´ | RecA¯ | T7 RNA Polyme -rase |
Cytoplasmic disulfide bond formation(5) |
Drug Resistance(6) |
| NEB Turbo | C2984 | 1-3×109 | K12 | √ | √ | √ | √ | √ | – | √ | – | √ | – | – | – | nit |
| NEB 5-alpha | C2987 | 1-3×109 | K12 | √ | √ | √ | √ | – | – | √ | – | – | √ | – | – | none |
| NEB 5-alpha F’Iq |
C2992 | 1-3×109 | K12 | √ | √ | √ | √ | √ | – | √ | – | √ | √ | – | – | tet |
| NEB 10-beta | C3019 | 1-3×109 | K12 | √ | √ | √ | √ | – | – | √ | – | – | √ | – | – | str |
| dam-/dcm- | C2925 | 1-3×106 | K12 | √ | √ | √ | – | – | – | √ | – | – | – | – | – | cam, str, nit |
| NEB Stable | C3040 | 1-3×109 | K12 | √ | √ | √ | √ | √ | – | √ | – | √ | √ | – | – | tet, str |
| Protein Expression Strain Properties | ||||||||||||||||
| NEB Express | C2523 | 0.6-1×109 | B | √ | √ | – | – | – | – | √ | √ | – | – | – | – | nit |
| NEB Express Iq | C3037 | 0.6-1×109 | B | √ | √ | – | – | √ | – | √ | √ | – | – | – | – | cam, nit |
| T7 Express | C2566 | 0.6-1×109 | B | √ | √ | – | – | – | – | √ | √ | – | – | √ | – | nit |
| T7 Express lysY |
C3010 | 0.6-1×109 | B | √ | √ | – | – | – | √ | √ | √ | – | – | √ | – | cam, nit |
| T7 Express lysY/Iq |
C3013 | 0.6-1×109 | B | √ | √ | – | – | √ | √ | √ | √ | – | – | √ | – | cam, nit |
| T7 Express Crystal |
C3022 | 0.6-1×109 | B | √ | √ | – | – | – | – | √ | √ | – | – | √ | – | nit |
| SHuffle T7 | C3026 | 1 x 106 | K12 | √ | – | – | – | √ | – | – | – | √ | – | √ | √ | str, spec |
| SHuffle Express |
C3028 | 1 x 107 | B | √ | – | – | – | √ | – | √ | √ | – | – | – | √ | spec |
| SHuffle T7 Express |
C3029 | 1 x 107 | B | √ | – | – | – | √ | – | √ | √ | – | – | √ | √ | spec(7) |
| SHuffle T7 Express lysY |
C3030 | 1 x 107 | B | √ | – | – | – | √ | √ | √ | √ | – | – | √ | √ | cam, spec(7) |
| BL21 | C2530 | 1-5×107 | B | √ | – | – | – | – | – | – | √ | – | – | – | – | – |
| BL21(DE3) | C2527 | 1-5×107 | B | √ | – | – | – | – | – | – | √ | – | – | √ | – | – |
| NiCo21(DE3) | C2529 | 1-5×107 | B | √ | – | – | – | – | – | – | √ | – | – | √ | – | – |
| Lemo21(DE3) | C2528 | 1-3×107 | B | √ | – | – | – | – | √ | – | √ | – | – | √ | – | cam |
Further information can be found in our Technical Resources section or at neb.com
