Protein Expression

Protein expression can be a very complex, multi-factorial process. Each protein requires a specific intracellular environment to correctly achieve its secondary and tertiary structures. Proteins may also require post-translational modifications or insertion into a cellular membrane for proper function. Other proteins, once expressed, may be toxic to the host. Thus, no single solution exists for the successful production of all recombinant proteins, and a broad range of expression tools is needed to ensure the successful expression of your target protein.

Our NEBExpress portfolio of products includes solutions for expression and purification, and is supported by access to scientists with over 40 years of experience in developing and using recombinant protein technologies in E. coli. We use these solutions in our own research and manufacturing processes, and know that quality and performance are critical – all of our products are stringently tested so that you can be sure they will work optimally for your solution, just as we rely on them to work in ours.

Video: Expression of difficult Proteins

Protein Expression Systems:

Generate analytical amounts of protein with our cell-free expression systems

Generate and purify high yields of recombinant proteins

  • The NEBExpress MBP Fusion and Purification System utilize a pMAL vector and the malE gene for the expression of MBP-fusion proteins, which can be isolated by a one-step affinity purification
  • The IMPACT Kit utilizes engineered protein splicing elements (inteins) to purify recombinant proteins in a single step
  • For analysis, try our protein standards, which are available unstained, prestained, or with two colors for easy identification

Optimized cells for protein expression

Eukaryotic protein expression

Purify tagged proteins with our magnetic beads, columns and resins

Further information can be found in our Technical Resources section or at