Not only are proteins a major structural component of living systems, they can also be effector molecules whose states determine downstream activities. Therefore, studying the protein complement within a cell can reveal the mechanisms behind many of the cell’s responses to its environment. Given the vast number of applications for protein analysis, several tools and methods for its study exist; determining the correct method for your application is paramount to success.
NEB offers a wide range of enzymes, like proteases, phosphatases and kinases, for protein analysis.
Phage display technology is an in vitro screening technique for identifying ligands for proteins and other macromolecules. At the crux of phage display technology is the ability to express peptide or protein sequences as fusions to the coat proteins of a bacteriophage. Libraries of phage-displayed peptides or proteins are thereby physically linked to their encoding nucleic acid, allowing selection of binding partners for myriad target types by iterative rounds of in vitro panning and amplification, followed by DNA sequencing. Libraries of over a billion members can be screened in a matter of days, offering an efficient alternative to more traditional methods of epitope mapping, receptor ligand identification, or protein evolution.
Furthermore, NEB offers Protein Markers and Ladders, products for protein labeling and Protein Pull-Down Assays.
NEB offers a selection of highly pure protein markers and ladders available in unstained and prestained formats. Sizes range from 2.3 to 250 kDa which is ideal for accurate molecular weight determination for a wide range of expressed proteins. NEB markers and ladders provide uniform band intensities, convenient band spacing and easy-to-identify reference bands.
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Further information can be found in our Technical Resources section or at neb.com