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Glycosidases

New England Biolabs (NEB) offers a selection of endoglycosidases, exoglycosidases, and heparinases for glycobiology research. Many of these reagents are recombinant, and all undergo several quality control assays, enabling us to provide products with lower unit cost, high purity and reduced lot-to-lot variation. All of our glycosidases are tested for contaminants. Since p-nitrophenyl-glycosides are not hydrolyzed by some exoglycosidases, we use only fluorescently-labeled oligosaccharides to screen for contaminating glycosidases.

And, to simplify workflows and digestions with two or more enzymes, the number of glycosidase reaction buffers was recently decreased from 6 to 3.

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Remove-iT™ Endoglycosidases

Remove-iT PNGase F is an amidase which cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides from N-linked glycoproteins. Remove-iT PNGase F is tagged with a chitin binding domain (CBD) for easy removal from a reaction and is supplied glycerol free for optimal performance in HPLC and MS intensive methods.

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Application Note

Remove-iT® PNGase F: Effective Release and Recovery of Neutral and Sialylated N-glycans

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Remove-iT Endo S is an endoglycosidase with a uniquely high specificity for removing N-linked glycans from the chitobiose core of the heavy chain of native IgG. Remove-iT Endo S is tagged with a chitin binding domain (CBD) for easy removal from a reaction and is supplied glycerol free for optimal performance in HPLC and MS intensive methods.specificity_endos

Remove-iT Endo D , also known as Endoglycosidase D, is a recombinant glycosidase, which cleaves within the chitobiose core of paucimannose N-linked glycans, with or without extensions in the antennae. Remove-iT Endo D is tagged with a chitin binding domain (CBD) for easy removal from a reaction, and is supplied glycerol-free for optimal performance in HPLC and MS- intensive methods.

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Protein Deglycosylation Mix

The Protein Deglycosylation Mix kit contains all of the enzymes, reagents, and controls needed to remove almost all N-linked and simple O-linked glycans as well as some complex O-linked glycans. This kit contains enzyme sufficient for 20 reactions or the cleavage of as much as 2 mg of glycoprotein.

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Visit our shop to view our full range of Glycobiology products.

Further information can be found in our Technical Resources section or at neb.com