Phusion® Technology
Phusion DNA Polymerase brings together a novel Pyrococcus-like enzyme with a processivity-enhancing domain and generates PCR products with accuracy and speed previously unattainable with a single enzyme, even on your most difficult templates. Additionally, Phusion DNA Polymerase is capable of amplifying long templates. In the quality control tests of Phusion DNA Polymerase, 7.5 kb genomic DNA and 20 kb λ DNA are routinely amplified. The unique structure and characteristics of Phusion DNA Polymerase make it a superior choice for cloning. The Phusion High-Fidelity DNA Polymerase sets a new standard for PCR performance.
Advantages:
- Extreme Fidelity (50X greater than Taq)
- High Speed – Extension times are dramatically reduced (10X faster than Pfu)
- Robust Reactions – Maximal success with minimal optimization
- High Yield – Increased product yield with minimal enzyme amount
NEB Tm Calculator: Determine your primers’ optimal annealing temperature.
3.8 kb fragment was amplified from 50 ng of Jurkat gDNA using different polymerases. Reactions were carried out according to the manufacter’s recommended conditions (cycling conditions are shown below). Extension times are indicated (in minutes).. Ladder L is a 1 kb DNA Ladder #N3232.
Available Products
| PRODUCT | REF. | SIZE | PRICE | |
|---|---|---|---|---|
| Phusion® High-Fidelity DNA Polymerase | M0530S/L | 100/500 units |  |
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| Phusion® High-Fidelity HF PCR Master Mix | M0531S/L | 100/500 rxn (à 50 µl volume) |  |
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| Phusion® High-Fidelity GC PCR Master Mix | M0532S/L | 100/500 rxn (à 50 µl volume) | |
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| Phusion® High-Fidelity PCR Kit | E0553S/L | 50/200 units | |
|
| Phusion Hot Start Flex Polymerases : | ||||
| Phusion® Hot Start Flex DNA Polymerase | M0535S/L | 100/500 units | |
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| Phusion® Hot Start Flex 2X Master Mix | M0536S/L | 100/500 units | |
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| Companion products : | ||||
| Deoxynucleotide Solutions, Mix | N0447S/L | 8 µmol each/ 40 µmol each | |
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Legal notice
Licenses/Patents/Disclaimers:
Phusion® DNA Polymerase was developed by Finnzymes Oy, now a part of Thermo Fisher Scientific. This product is manufactured by New England Biolabs, Inc. under agreement with, and under the performance specifications of Thermo Fisher Scientific. Phusion® is a registered trademark and property of Thermo Fisher Scientific.
Notice to purchaser: Limited license.
The purchase price of this product includes a limited, non-transferable license under U.S. and foreign patents owned by BIO-RAD Laboratories, Inc., to use this product. No other license under these patents is conveyed expressly or by implication to the purchaser by the purchase of this product.
The purchase price of this product includes a limited, non-transferable license under a patent owned by New England Biolabs, Inc., to use this product. No other license under this patent is conveyed expressly or by implication to the purchaser by the purchase of this product.
Nucleic acid-based aptamers for use with thermophilic DNA polymerases are licensed exclusively by New England Biolabs, Inc. from SomaLogic, Inc. (See Patent Nos. 5,475,096; 5,670,637; 5,696,249; 5,874,557; and 5,693,502). New England Biolabs, Inc. gives the Buyer/User a non-exclusive license to use the aptamer-based Phusion Hot Start Flex DNA Polymerase for RESEARCH PURPOSES ONLY. Commercial use of the aptamer-based Phusion Hot Start Flex DNA Polymerase requires a license from New England Biolabs, Inc. Please contact busdev@neb.com for more information.
Further information can be found in our Technical Resources section or at neb.com
