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Luna® Universal qPCR & RT-qPCR

Fluorescence-based quantitative real-time PCR (qPCR) is the gold standard for the detection and quantification of nucleic acids due to its sensitivity and specificity. Luna products from NEB are optimized for qPCR or RT-qPCR, and are available for either intercalating dye or probe-based detection methods. All Luna products provide robust performance on diverse sample sources and target types.

Each Hot Start Taq-based Luna qPCR master mix has been formulated with a unique passive reference dye that is compatible across a wide variety of instrument platforms, including those that require a ROX reference signal. This means that no additional components are required to ensure machine compatibility. The mixes also contain dUTP, enabling carryover prevention when reactions are treated with NEB’s Antarctic Thermolabile UDG (NEB #M0372). A blue visible dye assists in tracking the reagents when pipetting into clear, multi-welled PCR plates. The reverse transcriptase, featured in the Luna RT-qPCR products is a novel, engineered WarmStart® enzyme developed for robust performance and increased thermostability. These features, combined with rapid, sensitive and precise real-time qPCR performance, make Luna the universal choice for all your qPCR and RT-qPCR experiments.

LUNA qPCROverview of qPCR
Learn the basics of qPCR in this short animation.

Experience best-in-class performance

  • All Luna products have undergone rigorous testing to optimize specificity, sensitivity, accuracy and reproducibility
  • Products perform consistently across a wide variety of sample sources
  • A comprehensive evaluation of commercially-available qPCR and RT-qPCR reagents demonstrates superior performance of Luna products
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Optimize your RT-qPCR with Luna WarmStart® Reverse Transcriptase

  • Novel, thermostable reverse transcriptase (RT) improves performance
  • WarmStart RT paired with Hot Start Taq increases reaction specificity and robustness
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Make a simpler choice

  • One product per application simplifies selection
  • Convenient master mix formats and user-friendly protocols simplify reaction setup
  • Non-interfering, visible tracking dye helps to eliminate pipetting errors
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Available products

NEW Luna® Cell Ready One-Step RT-qPCR Kit E3030S 100 rxns Webshop Information Produit
NEW Luna® Cell Ready Probe One-Step RT-qPCR Kit E3031S 100 rxns Webshop Information Produit
NEW Luna® Cell Ready Lysis Module E3032S 100 rxns Webshop Information Produit
Luna® Universal qPCR Master Mix M3003S 200 rxns (2x1ml) Webshop Produktinformationen
M3003L 500 rxns (5x1ml) Webshop Produktinformationen
M3003X 1000 rxns (10x1ml) Webshop Produktinformationen
M3003E 2500 rxns (1x25ml) Webshop Produktinformationen
Luna® Universal Probe qPCR Master Mix M3004S 200 rxns (2x1ml) Webshop Produktinformationen
M3004L 500 rxns (5x1ml) Webshop Produktinformationen
M3004X 1000 rxns (10x1ml) Webshop Produktinformationen
M3004E 2500 rxns (1x25ml) Webshop Produktinformationen
Luna® Universal One-Step RT-qPCR Kit E3005S 200 rxns Webshop Produktinformationen
E3005L 500 rxns Webshop Produktinformationen
E3005X 1000 rxns Webshop Produktinformationen
E3005E 2500 rxns Webshop Produktinformationen
Luna® Universal Probe One-Step RT-qPCR Kit E3006S 200 rxns Webshop Produktinformationen
E3006L 500 rxns Webshop Produktinformationen
E3006X 1000 rxns Webshop Produktinformationen
E3006E 2500 rxns Webshop Produktinformationen
NEW LunaScript RT SuperMix Kit E3010S 25 rxns Webshop Produktinformationen
E3010L 100 rxns Webshop Produktinformationen

Further information can be found in our Technical Resources section or at