Taq DNA Polymerase
Taq DNA Polymerase is commonly used for routine PCR applications in many molecular biology labs.
For your “daily” PCR we recommend NEBs reliable Taq DNA Polymerase, which is available in various Buffer- and Enzyme-formulations.
- Isolated from a recombinant source
- Supplied with 10X Reaction Buffer
- Robust and reliable reactions
- Tolerates a wide range of templates
- Incorporates dUTP, dITP and fluorescently-labeled nucleotides
- Exceptional value in terms of cost per unit
Hot Start Taq DNA Polymerase
Hot Start Taq DNA Polymerase is a mixture of Taq DNA Polymerase and an aptamer-based inhibitor. The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 45°C, but releases the enzyme during normal cycling conditions, allowing reactions to be set up at room temperature. This aptamer-based hot start does not require a separate high temperature incubation step to activate the enzyme.
NEB’s Hot Start Taq shows excellent yields while being highly specific
| Manufacturer | DNA Polymerase | Hot Start Inhibitor | Activation time** | Performance* | |
| Specificity | Yield | ||||
| Applied Biosystens | AmpliTaq Gold 360 | Chem | 10 min |  |
|
| Invitrogen | Platinum Taq | Ab | 30 sec |  |
 |
| NEB | Hot Start Taq | Aptamer | None |  |
|
| Promega | GoTaq Hot | Ab | 2 min | |
|
| Qiagen | Hot Star Taq | Chem | 15 min | |
|
| Roche | FastStart Taq | Chem | 4 min | |
|
| Sigma | JumpStart Taq | Ab | 1 min | |
|
| Thermo Scientific | Maxima Hot Start Taq | Chem | 4 min | |
|
*Performance compared to all enzymes tested. Results are from triplicate reactions on a series of human genomic amplicons. Performance was scored for both yield and desired product. **Time required to activate hot start enzymes may include initiation denaturation time, if not specified.
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Further information can be found in our Technical Resources section or at neb.com
