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Luna® – Optimize RT-qPCR with unique WarmStart® technology

The Luna RT-qPCR kits contain a novel, in silico-designed reverse transcriptase (RT) engineered for improved performance. Both the Luna WarmStart Reverse Transcriptase and Hot Start Taq DNA Polymerase, included in these kits, utilize a temperature-sensitive, reversible aptamer, which inhibits activity below 45°C. This enables room temperature reaction setup and prevents undesired non-specific activity. Furthermore, the WarmStart RT has increased thermostability, improving performance at higher reaction temperatures.

Improve performance with our novel, thermostable reverse transcriptase

  • Robust RT delivers improved specificity, sensitivity and efficiency
  • Increased thermostability adds flexibility to reaction setup and improved specificity

Luna WarmStart Reverse Transcriptase exhibits exceptional sensitivity, reproducibility and RT-qPCR performance

RT-qPCR targeting human GAPDH was performed using the Luna Universal One-Step RT-qPCR Kit, featuring Luna WarmStart Reverse Transcriptase, over an 8-log range of input template concentrations (0.1 pg – 1 μg Jurkat total RNA) with 8 replicates at each concentration. NTC = non-template control.

Achieve better control and better results: WarmStart Luna RT paired with Hot Start Taq

  • WarmStart/Hot Start technology ensures tight control and prevents spurious amplification
  • Unique WarmStart technology is not available with other non-NEB commercially-available products

WarmStart Luna RT prevents spurious amplification resulting from room-temperature  pre-incubation

RT-qPCR targeting human ribosomal protein L32 was performed before and after a 24-hour incubation at room temperature, with triplicate reactions for a 5-log range of input human (Jurkat) total RNA and a non-template control. The Luna Universal One-Step RT-qPCR Kit featuring Luna WarmStart Reverse Transcriptase exhibited robust performance and no detectible non-template amplification, either with or without a 24 hour 25°C pre-incubation, while the ABI 1-Step Kit, featuring a non-WarmStart reverse transcriptase, exhibited significant non-template amplification.

Greater thermostability means greater versatility

  • Increased RT reaction temperature is ideal for difficult targets

The increased thermostability of WarmStart Luna Reverse Transcriptase improves performance at increased reaction temperatures

RT-qPCR experiments targeting human ribosomal protein L32 RNA were performed in triplicate over a 5-log range of input human (Jurkat) total RNA (5 pg – 50 ng) using an initial 10 min RT step performed at 50°C – 60°C, as indicated.

A. Luna WarmStart Reverse Transcriptase (recommended incubation temperature: 55°C) exhibited rapid Cq values (bar graph) and robust RT-qPCR performance (amplification plots) at each temperature, indicating that efficient reverse transcription was not perturbed by reaction temperature alterations.

B. In contrast, a commercially available MMLV (recommended incubation temperature: 50°C) exhibited delayed (increased) Cq values, poorer performance, and loss of low-input detection at elevated temperatures, consistent with loss of RT activity.

One or more of these products are covered by patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information, please email us at gbd@neb.com. The use of these products may require you to obtain additional third party intellectual property rights for certain applications.

SYBR® is a registered trademark of Molecular Probes, Inc.
ARRAYSCRIPT™ is a trademark of Thermo Fisher Scientific,Inc.

Available products

PRODUCT REF. SIZE PRICE MORE INFOS
Luna® Universal qPCR Master Mix M3003S 200 rxns (2x1ml) In den Shop wechseln Produktinformationen
M3003L 500 rxns (5x1ml) In den Shop wechseln Produktinformationen
M3003X 1000 rxns (10x1ml) In den Shop wechseln Produktinformationen
M3003E 2500 rxns (1x25ml) In den Shop wechseln Produktinformationen
Luna® Universal Probe qPCR Master Mix M3004S 200 rxns (2x1ml) In den Shop wechseln Produktinformationen
M3004L 500 rxns (5x1ml) In den Shop wechseln Produktinformationen
M3004X 1000 rxns (10x1ml) In den Shop wechseln Produktinformationen
M3004E 2500 rxns (1x25ml) In den Shop wechseln Produktinformationen
Luna® Universal One-Step RT-qPCR Kit E3005S 200 rxns In den Shop wechseln Produktinformationen
E3005L 500 rxns In den Shop wechseln Produktinformationen
E3005X 1000 rxns In den Shop wechseln Produktinformationen
E3005E 2500 rxns In den Shop wechseln Produktinformationen
Luna® Universal Probe One-Step RT-qPCR Kit E3006S 200 rxns In den Shop wechseln Produktinformationen
E3006L 500 rxns In den Shop wechseln Produktinformationen
E3006X 1000 rxns In den Shop wechseln Produktinformationen
E3006E 2500 rxns In den Shop wechseln Produktinformationen
NEW LunaScript RT SuperMix Kit E3010S 25 rxns In den Shop wechseln Produktinformationen
E3010L 100 rxns In den Shop wechseln Produktinformationen

Further information can be found in our Technical Resources section or at neb.com