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OneTaq DNA Polymerase

OneTaq DNA Polymerase

OneTaq DNA Polymerase is an optimized blend of Taq and Deep Vent DNA polymerases for use with routine and difficult PCR experiments. The 3´→ 5´ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase. The OneTaq Reaction Buffers and High GC Enhancer have been formulated for robust yields with minimal optimization, regardless of a template’s GC content.

OneTaq DNA Polymerase is supplied with two 5X buffers (Standard and GC), as well as a High GC Enhancer solution. For most routine and/or AT-rich amplicons (Lambda, etc.) or complex amplicons with up to ~65% GC content, OneTaq Standard Reaction Buffer provides robust amplification. For GC-rich amplicons, the OneTaq GC Reaction Buffer can improve both performance and yield. For particularly high GC or difficult amplicons, the OneTaq High GC Enhancer can be added at a final concentration of 10–20% to reactions containing OneTaq GC Reaction Buffer.

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New:
OneTaq Quick-Load DNA Polymerase – After PCR directly onto the gel!
Besides the conventional reaction buffer this new variant contains a colored Quick-Load reaction buffer for direct gel loading on agarose gels after PCR. OneTaq Quick-Load DNA Polymerase is therefore particularly well suited for routine PCR like genotyping, colony PCR etc. (up to 6 kb and ca. 55% GC content).
Quick – reliable – favorable!

Whichever option you choose – the OneTaq DNA Polymerase is the unmatched standard for your everyday routine PCR.

Advantages:

  • Ideal for routine, AT- and GC-rich PCR Applications

Applications:

  • High Sensitivity PCR
  • High Throughput PCR
  • Routine PCR
  • GC-rich PCR
  • AT-rich PCR
  • Colony PCR
  • Long PCR (up to ~6 kb genomic)

NEB_ColonyPCR_ApplicationNote-100x130

Application Note:

“Robust Colony PCR from Multiple E. coli Strains using OneTaq® Quick-Load® Master Mixes”

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Amplification of a selection of sequences with varying GC content from human and C. elegansgenomic DNA using OneTaq DNA Polymerase. GC content is indicated above gel. Marker M is the 1 kb DNA Ladder .

OneTaq Hot-Start DNA Polymerase

OneTaq® Hot Start DNA Polymerase is an optimized blend of Taq and Deep VentR™ DNA polymerases combined with an aptamer-based inhibitor.
The inhibitor binds reversibly, blocking polymerase activity at temperatures below 45°C. The polymerase is activated during normal cycling conditions, allowing reactions to be set up at room temperature. OneTaq Hot Start DNA Polymerase does not require a separate high temperature incubation step to activate the enzyme and can be used in typical Taq-based cycling protocols.

Prolonged incubation times at room temperature have no influence on the performance of OneTaq® Hot Start DNA Polymerase

Amplification of a selection of sequences with varying GC content from human and C. elegans genomic DNA using OneTaq Hot Start DNA Polymerase. The presence or absence of an extended room temperature incubation does not affect performance. GC content is indicated below gel. Marker M is the 1 kb DNA Ladder .

Comparison of recommended activation steps of commercially available Hot Start DNA Polymerases

MANUFACTURER ENZYME ACTIVATION STEP* HOT START FORM
Applied BioSystems AmpliTaq Gold® 360 10′, 95°C Modified
Invitrogen Platinum® Taq 30″–2′, 94°C Ab
Promega GoTaq® Hot Start 2′, 94–95°C Ab
Qiagen HotStarTaq 15′, 95°C Modified
Roche FastStart Taq 4′, 95°C Modified
Sigma JumpStart™ Taq 1′, 94°C Ab
Thermo Fisher Thermo-Start Taq 15′, 95°C Modified
NEB OneTaq None Aptamer

Product table

PRODUCT Prod.Nr. SIZE PRICE
OneTaq® DNA Polymerase M0480 S 200 units shop_icon
M0480 L 1000 units shop_icon
M0480 X 5000 units shop_icon
OneTaq® Hot Start DNA Polymerase M0481 S 200 units shop_icon
M0481 L 1000 units shop_icon
M0481 X 5000 units shop_icon
OneTaq® 2X Master Mix with Standard Buffer M0482 S 100 rxns shop_icon
M0482 L 500 rxns shop_icon
OneTaq® 2X Master Mix with GC Buffer M0483 S 100 rxns shop_icon
M0483 L 500 rxns shop_icon
OneTaq® Hot Start 2X Master Mix with Standard Buffer M0484 S 100 rxns shop_icon
M0484 L 500 rxns shop_icon
OneTaq® Hot Start 2X Master Mix with GC Buffer M0485 S 100 rxns shop_icon
M0485 L 500 rxns shop_icon
OneTaq® Quick-Load 2X Master Mix with Standard Buffer M0486 S 100 rxns shop_icon
M0486 L 500 rxns shop_icon
OneTaq® Hot Start Quick-Load 2X Master Mix, Standard Buffer M0488 S 100 rxns shop_icon
M0488 L 500 rxns shop_icon
OneTaq® Hot Start Quick-Load 2X Master Mix with GC Buffer M0489 S 100 rxns shop_icon
M0489 L 500 rxns shop_icon
OneTaq® Quick-Load® DNA Polymerase M0509 S 100 units shop_icon
M0509 L 500 units shop_icon
M0509 X 2500 units shop_icon

Further information can be found in our Technical Resources section or at neb.com