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Taq DNA Polymerases

Taq DNA Polymerase

Taq DNA Polymerase is commonly used for routine PCR applications in many molecular biology labs.

For your “daily” PCR we recommend NEBs reliable Taq DNA Polymerase, which is available in various Buffer- and Enzyme-formulations.

Advantages:
  • Isolated from a recombinant source
  • Supplied with 10X Reaction Buffer
  • Robust and reliable reactions
  • Tolerates a wide range of templates
  • Incorporates dUTP, dITP and fluorescently-labeled nucleotides
  • Exceptional value in terms of cost per unit

Hot Start Taq DNA Polymerase

Hot Start Taq DNA Polymerase is a mixture of Taq DNA Polymerase and an aptamer-based inhibitor. The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 45°C, but releases the enzyme during normal cycling conditions, allowing reactions to be set up at room temperature. This aptamer-based hot start does not require a separate high temperature incubation step to activate the enzyme.

NEB’s Hot Start Taq shows excellent yields while being highly specific

Manufacturer DNA Polymerase Hot Start Inhibitor Activation time** Performance*
Specificity Yield
Applied Biosystens AmpliTaq Gold 360 Chem 10 min NEB HSTaq_3 NEB HSTaq_3
Invitrogen Platinum Taq  Ab 30 sec NEB HSTaq_1 NEB HSTaq_2
NEB Hot Start Taq Aptamer None NEB HSTaq_4 NEB HSTaq_3
Promega GoTaq Hot Ab 2 min NEB HSTaq_1 NEB HSTaq_1
Qiagen Hot Star Taq Chem 15 min NEB HSTaq_1 NEB HSTaq_3
Roche FastStart Taq Chem 4 min NEB HSTaq_3 NEB HSTaq_4
Sigma JumpStart Taq Ab 1 min NEB HSTaq_3 NEB HSTaq_4
Thermo Scientific Maxima Hot Start Taq Chem 4 min NEB HSTaq_2 NEB HSTaq_2
NEB_PCR_HSTaq_legend

*Performance compared to all enzymes tested. Results are from triplicate reactions on a series of human genomic amplicons. Performance was scored for both yield and desired product. **Time required to activate hot start enzymes may include initiation denaturation time, if not specified.

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Further information can be found in our Technical Resources section or at neb.com