Cloning / Synthetic Biology
Tools
- NEBCloner
- NEBuilder
- NEB Golden Gate Assembly Tool
- Double Digest Finder
- NEB Cutter
- DNA Sequences and Maps Tool
- Enzyme Finder
Selection Tools
Troubleshooting Guides
- Troubleshooting Guide for Cloning
- Restriction Enzyme Troubleshooting Guide
- Troubleshooting Guide for Ligases
- Troubleshooting Guide for End Modification
Usage Guidelines / Tipps
- Restriction Enzyme Tips
- Optimizing Restriction Endonuclease Reactions
- NEBuffer Activity/Performance Chart with Restriction Enzymes
- Activity of Restriction Enzymes in a Q5®, Taq or Phusion PCR Mix
- Cloning Plasmids and DNAs Overview
- Compatible Cohesive Ends and Generation of New Restriction Sites
- Dam-Dcm and CpG Methylation
- Frequencies of Restriction Sites
- Recleavable Blunt Ends
- Recleavable Filled-in 5′ Overhangs
- Cleavage of Supercoiled DNA
- Activity at 37°C for Restriction Enzymes with Alternate Incubation Temperatures
- Digestion of Agarose-Embedded DNA: Info for Specific Enzymes
- Restriction Endonucleases – Survival in a Reaction
- Traditional Cloning Quick Guide
- Tips for Maximizing Ligation Efficiencies
- End-Modification Tips
FAQs
Application Notes
Video Tutorials
Restriction Enzyme Digest Problem: Too Many DNA Bands
Why is My Restriction Enzyme Not Cutting DNA?
Introduction to NEBuilder HiFi DNA Assembly
Primer Design and Fragment Assembly Using NEBuilder HiFi DNA Assembly or Gibson Assembly
Visit the NEB YouTube Channel for more video tutorials.
PCR and DNA Amplification
Tools
Selection Tools
Troubleshooting Guides
Usage Guidelines / Tipps
- Guidelines for PCR Optimization with Taq DNA Polymerase
- Guidelines for PCR Optimization with OneTaq® and OneTaq® Hot Start DNA Polymerases
- Guidelines for PCR Optimization with Thermophilic DNA Polymerases
FAQs
Application Notes
Video Tutorials
Overview of Traditional Cloning
DNA Blunting Tutorial
DNA Ligation
Visit the NEB YouTube Channel for more video tutorials.
Next Generation Sequencing
Tools
Selection Tools
Usage Guidelines / Tipps
Application Notes
Video Tutorials
Tips for Bead based Clean ups and Size Selection
12 Quick Tips for NGS Library Preparation
Tips for Optimizing DNA Inputs in NGS Library Construction
Tips for Optimizing RNA Inputs in NGS Library Construction
Visit the NEB YouTube Channel for more video tutorials.
RNA Biology
Selection Tools
- RNA Polymerase Selection Chart
- RNA Ligase Selection Chart
- cDNA Synthesis Selection Chart
- Recommended HiScribe RNA Synthesis Kits by Application
Usage Guidelines / Tipps
Video Tutorials
In vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection
Visit the NEB YouTube Channel for more video tutorials.
Competent Cells
Selection Tools
Troubleshooting Guides
Usage Guidelines / Tipps
Video Tutorials
The Mechanism of Transformation with Competent Cells
Visit the NEB YouTube Channel for more video tutorials.
Glycobiology
Tools
Usage Guidelines / Tipps
FAQs
Application Notes
Video Tutorials
Identification and Characterization of Protein Glycosylation
Tools for Glycoprotein Analysis
Visit the NEB YouTube Channel for more video tutorials.
DNA / RNA Markers and Ladders
Selection Tools
Proteinexpression
Video Tutorials
Solutions for the Expression of Difficult Proteins
Visit the NEB YouTube Channel for more video tutorials.
Epigenetics
Video Tutorials
Epigenetics Tutorial
What Is Epigenetics?
Visit the NEB YouTube Channel for more video tutorials.
Cellular Analysis (SNAP-Tag)
Selection Tools
Troubleshooting Guides
FAQs
Application Notes
- Labeling of Escherichia coli Expressed SNAP-tag® Fusion Proteins
- Labeling and Imaging of Cell Surface Receptors Mediated by SNAP-tag®
- Simultaneous Fluorescent Labeling of Proteins in Living Cells
- Simultaneous Labeling of Two Proteins in Live Cells
- SNAPf based pulse labeling for analysis of protein turnover in living cells
Video Tutorials
SNAP-Tag Overview Tutorial
SNAP-tag Tutorial
Visit the NEB YouTube Channel for more video tutorials.
