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Ligase Master Mixes

Blunt/TA Ligase Master Mix

The Blunt/TA Ligase Master Mix (NEB #M0367) is specifically formulated to improve ligation and transformation of both blunt-end and single-base overhang substrates. This master mix format simplifies reaction setup, ensuring robust, rapid ligation with an optimized ratio of enzyme and buffer components. Reactions can be used to directly transform many strains of chemically competent E. coli without dilution.

Blunt/TA Ligase Master Mix Outperforms the Competition:

duplicate_ligation_reactions2

Duplicate ligation reactions of blunt or T/A vector/insert pairs were set upaccording to the master mix vendors’ suggestions. Equal amounts of ligated DNA were used to transform NEB 10-beta Competent E. coli (NEB #C3019) and triplicate plating was performed. Transformation results were averaged and graphed as a percentage of the highest performing reaction, T/A ligation using the Blunt/TA Ligase Master Mix.

Instant Sticky-end Ligase Master Mix

Complete with T4 DNA Ligase (NEB #M0202), proprietary ligation enhancer and optimized reaction buffer, the Instant Sticky-end Ligase Master Mix (NEB #M0370) enables instant, incubation- free ligation. Specifically formulated to rapidly ligate sticky-end (2–4 bp) substrates and improve transformation, this mix simplifies reaction set-up and reduces the time needed for routine ligations. Reactions can be used to directly transform many strains of chemically competent E. coli without dilution.

Instant Sticky-end Ligase Master Mix Offers Robust Ligation with No Incubation Step

Reactions containing 20 ng of pUC19 digested with SacI/SphI, and a 3-foldmolar excess of a 1.6 kb fragment from a different plasmid with compatible ends were set up using the Instant Sticky-end Ligase Master Mix. Without any additional incubation time, 2 µl were immediately withdrawn and used to transform a 50 µl aliquot of NEB 10-beta Competent E. coli (NEB #C3019). A 50 µl aliquot of the 1 ml outgrowth was plated onto a selective plate and incubated overnight at 37°C. Results show that several hundred colonies wereproduced.

P R O D U C T T A B L E

PRODUCT Prod.Nr.: SIZE PRICE
Blunt/TA Ligase Master Mix M0367 S 50 reactions shop_icon
M0367 L 250 reactions shop_icon
Instant Sticky-end Master Mix M0370 S 50 reactions shop_icon
M0370 L 250 reactions shop_icon
ElectroLigase™ M0369 S 50 reactions shop_icon
T4 DNA Ligase M0202 S 20.000 units shop_icon
M0202 L 100.000 units shop_icon
Quick Ligation Kit M2200 S 30 reactions shop_icon
M2200 L 150 reactions shop_icon

 

PRODUCT Prod.Nr.: SIZE PRICE
T3 DNA Ligase M0317 S 100.000 units shop_icon
M0317 L 750.000 units shop_icon
T7 DNA Ligase M0318 S 100.000 units shop_icon
M0318 L 750.000 units shop_icon
E. coli DNA Ligase M0205 S 200 units shop_icon
M0205 L 1.000 units shop_icon
Taq DNA Ligase M0208 S 2.000 units shop_icon
M0208 L 10.000 units shop_icon
9°N™ DNA Ligase M0238 S 2.500 units shop_icon
M0238 L 12.500 units shop_icon

 

The Ligation Module for Library Preparation in Next-Gen-Sequencing

PRODUCT Prod.Nr.: SIZE PRICE
Quick Ligation Module E6056 S 20 reactions shop_icon
E6056 L 100 reactions shop_icon

Further information can be found in our Technical Resources section or at neb.com