NEB Synthetic Biology

Cloning 2.0 / DNA Assembly

“Cloning 2.0” – DNA Assembly and cloning products for synthetic biology

The field of synthetic biology is steadily gaining popularity, not only because of the international iGEM competition which was supported by NEB recently.

Due to the high interest in synthetic biology, new cloning and gene editing methods were developed. These methods allow to create large, artificial DNA structures through re-organisation, editing or assembly of genes.

NEB offers a variety of innovative „Cloning 2.0“ products to simplify your daily cloning workflows.

DNA Assembly Header

NEBuilder® HiFi DNA Assembly

NEBuilder HiFi DNA Assembly enables virtually error-free joining of DNA fragments, even those with 5´ and 3´ restriction enzyme mismatches. Available with (NEB #E5520) and without (NEB #E2621) competent E. coli, this flexible kit enables simple and fast Seamless Cloning utilizing a new proprietary high-fidelity polymerase. Find out why NEBuilder HiFi is the next generation of DNA assembly and cloning.

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NEB Golden Gate Assembly

Golden Gate Assembly

The efficient and seamless assembly of DNA fragments, commonly referred to as Golden Gate assembly, has its origins in 1996, when for the first time it was shown that multiple inserts could be assembled into a vector backbone using only the sequential or simultaneous activities of a single type IIS restriction enzyme and T4 DNA ligase.

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Further information can be found in our Technical Resources section or at