The NEBNext suite of products supports Illumina sequencing with library preparation tools that streamline workflows, minimize inputs, and improve library yields and diversity. NEBNext library preparation kits are available for DNA, ChIP DNA, RNA and small RNA samples. In addition to the extensive QCs on individual kit components, all NEBNext kits for Illumina are functionally validated by preparation of a library, followed by Illumina sequencing. NEBNext library preparation reagents are available in multiple kit and workflow formats, for maximum convenience and flexibility.
The NEBNext Advantages:
- Broad range of input amounts, from low ng to μg
- Fast workflows with minimal hands-on-time
- Gel-free workflows
- High yields
- High library diversity
- Minimized GC bias
- Convenient formats include kits and modules
- All reagents undergo stringent quality controls, plus sequencing validation
- Value pricing
Available Products
| Accessories | |||
| NEBNext® Magnetic Separation Rack | S1515S |  |
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| DNA for Illumina | |||
| NEW NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina® | E7805 | |
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| NEW NEBNext® Ultra™ II FS DNA Library Prep with Sample Purification Beads® | E6177 | |
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| NEW NEBNext® Ultra™ II FS DNA Module | E7810 | |
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| NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® | E7645 | |
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| NEBNext® Ultra™ II DNA Library Prep with Sample Purification Beads | E7103 | |
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| NEBNext® Ultra™ II Q5® Master Mix | M0544 | |
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| NEBNext® Ultra™ DNA Library Prep Kit for Illumina® | E7370 | |
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| NEBNext® DNA Library Prep Master Mix Set for Illumina® | E6040 | |
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| NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina® | E6240 | |
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| NEBNext® Library Quant kit for Illumina® | E7630 | |
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| RNA for Illumina | |||
| NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina® | E7760 | |
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| NEBNext® Ultra II Directional RNA Library Prep with Sample Purification Beads | E7765 | |
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| NEBNext® Ultra II RNA Library Prep Kit for Illumina® | E7770 | |
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| NEBNext® Ultra II RNA Library Prep with Sample Purification Beads | E7775 | |
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| NEBNext® Multiplex Small RNA Library Kit for Illumina® (Index Primers 1-48) | E7560 | |
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| NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 1) | E7300 | |
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| NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 2) | E7580 | |
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| NEBNext® Ultra™ RNA Library Prep Kit for Illumina® | E7530 | |
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| NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® | E7420 | |
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| NEBNext® Small RNA Library Prep Set for Illumina® (Multiplex Compatible) | E7330 | |
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| Primer & Adaptors | |||
| NEW NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs Set 2) | E6442 | |
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| NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 2) | E7780 | |
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| NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs) | E6440 | |
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| NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 3) | E7710 | |
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| NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 4) | E7730 | |
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| NEBNext® Multiplex Oligos for Illumina® (96 Index Primers) | E6609 | |
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| NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1) | E7335 | |
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| NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 2) | E7500 | |
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| NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 1), 96plex | E7600 | |
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| NEBNext® Singleplex Oligos for Illumina® Discontinued, please click on i to contact us for an alternative product |
E7350 | |
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| NEBNext Modules & Buffer for Illumina | |||
| for DNA | |||
| NEBNext® FFPE DNA Repair Mix | M6630 | |
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| NEBNext® Ultra™ II End Repair/dA-Tailing Module | E7546 | |
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| NEBNext® Ultra™ End Repair/dA-Tailing Module | E7442 | |
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| NEBNext® Ultra™ II Ligation Module | E7595 | |
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| NEBNext® Ultra™ Ligation Module | E7445 | |
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| NEBNext® End Repair Module | E6050 | |
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| NEBNext® Quick Ligation Module | E6056 | |
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| NEBNext® dA-Tailing Module | E6053 | |
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| for RNA | |||
| NEBNext® Ultra™ II RNA First Strand Synthesis Module | E7771 | |
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| NEBNext® Ultra™ Directional RNA Second Strand Synthesis Module | E7550 | |
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| NEBNext® Poly(A) mRNA Magnetic Isolation Module | E7490 | |
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| NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) | E6310 | |
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| NEW NEBNext® Globin & rRNA Depletion Kit (Human/Mouse/Rat) | E7750 | |
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| NEW NEBNext® Globin & rRNA Depletion Kit (Human/Mouse/Rat) with RNA Sample Purification Beads | E7755 | |
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| NEBNext® RNA First Strand Synthesis Module | E7525 | |
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| NEBNext® mRNA Second Strand Synthesis Module | E6111 | |
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| NEBNext® RNase III RNA Fragmentation Module | E6146 | |
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| NEBNext® Magnesium RNA Fragmentation Module | E6150 | |
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Further information can be found in our Technical Resources section or at neb.com
