NEBNext rRNA Depletion Kit
The NEBNext rRNA Depletion Kit (Human/Mouse/Rat) enables efficient removal of ribosomal RNA (rRNA) from total RNA for human, mouse and rat RNA samples. This kit works well for both low-quality, degraded RNA (including FFPE RNA) and high-quality, intact RNA. rRNA depletion with a broad range of input amounts (10 ng to 1 μg) is completed in approximately 2 hours, with less than 10 minutes of hands-on time.
- A single kit that performs reliably well for all of your RNA samples: --> FFPE (degraded) or high-quality (intact) RNA --> 10 ng to 1 μg input amounts
- Remove > 95% of rRNA, and obtain more relevant sequence reads from your sample
- Obtain a more complete transcriptome picture through retention of noncoding & incomplete RNAs that are lost with oligo d(T) poly(A) mRNA enrichment methods
- Suitable for use with human, mouse or rat samples
- Easily integrated upstream of any downstream random-primed cDNA synthesis protocol
- No more need to homebrew: A reliable & convenient NGS-validated kit for the “RNase H protocol” for rRNA depletion
Application Note: Selective depletion of abundant RNAs to enable transcriptome analysis of low-input and highly-degraded RNA from FFPE breast cancer samples
NEBNext rRNA Depletion Kit Workflow
Click to enlarge
New: NEBNext Globin & rRNA Depletion Kit
Globin mRNA and rRNA are highly abundant in blood samples, and their efficient and specific removal is desirable in order to reveal the biological significance of less abundant transcripts.
The NEBNext Globin & rRNA Depletion Kit (Human/Mouse/Rat) employs the NEBNext RNase H-based RNA depletion workflow to deplete globin mRNA, cytoplasmic rRNA and mitochondrial rRNA with human, mouse and rat samples, and is effective with intact and degraded RNA.
- Efficient, specific depletion of globin mRNA and rRNA
- Suitable for low-quality or high-quality RNA
- Compatible with a broad range of input amounts: 10 ng - 1 μg
- Optional integration with poly(A) mRNA isolation workflows for removal of globin RNAs, rRNAs, and noncoding RNAs
- Fast workflow: 2 hours, with less than 10 minutes hands-on time
- Also available with RNA Sample Purification Beads
New: NEBNext rRNA Depletion Kit (Bacteria)
Ribosomal RNA (rRNA) is highly abundant in bacterial samples, and its removal is desirable in order to reveal the biological significance of less abundant transcripts. Specific enrichment of bacterial mRNAs is challenging due to their lack of poly(A) tails, and so the converse – efficient and specific removal of bacterial rRNA – is necessary.
The NEBNext rRNA Depletion Kit (Bacteria) employs the NEBNext RNase H-based RNA depletion workflow to target removal of rRNA from gram-positive and gram-negative organisms. The method is effective with intact and degraded RNA, from monocultures or samples with mixed bacterial species (e.g., metatranscriptomic).
List of bacteria tested with the RNA Depletion Kit
- Efficient, specific depletion of bacterial rRNA (5S, 16S, 23S)
- Compatible with both gram-positive and gram-negative organisms
- Effective with monocultures and mix of bacterial species (e.g., metatranscriptome)
- Compatible with a broad range of input amounts: 10 ng - 1 µg
- Suitable for low-quality or high-quality RNA
- Fast workflow: 2 hours, with less than 10 minutes hands-on time
Research Assistant
Biomedical Research Institution
“The NEB Bacterial Depletion has depleted rRNA equally or better than our previous ribodepletion gold standard across a wide RIN quality range. We have been pleased with the flexibility of Total RNA input ranges and have routinely gotten effective ribodepletion at 100 ng Total RNA Input in both single isolates and metagenomic samples. The protocol is also more ergonomically friendly than bead based ribodepletion protocols. Of all the new bacterial ribodepletion methods we have tested, NEB was by far the best.”
New: NEBNext RNA Depletion Reagent Core Set
In RNA-seq, highly expressed transcripts with minimal biological interest, such as ribosomal RNA (rRNA) can dominate readouts and mask detection of more informative low-abundance transcripts. This challenge is amplified when working with sample types for which pre-designed RNA depletion kits are not available. The NEBNext RNA Depletion Core Reagent set provides a customized approach to deplete unwanted RNA from any organism, using probe sequences designed with the user-friendly NEBNext Custom RNA Depletion Design Tool.
The efficient RNase-H-based workflow, and close tiling of probes designed using the online tool, enables effective depletion from both low- and high-quality RNA, with a broad range of input amounts.
The kit is also available with RNAClean® beads.
- Customizable option to deplete unwanted RNA from any organism, using probe sequences designed with the NEBNext Custom RNA Depletion Design Tool.
- Compatible with a broad range of input amounts: 10 ng - 1 μg
- Suitable for low-quality or high-quality RNA
- Fast workflow: 2 hours, with less than 10 minutes hands-on time
Available products
| PRODUCT | REF. | SIZE | PRICE | |
|---|---|---|---|---|
| New NEBNext® RNA Depletion Core Reagent Set | E7865S | 6 rxns |  |
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| E7865L | 24 rxns | |
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| E7865X | 96 rxns | |
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| New NEBNext® RNA Depletion Core Reagent Set with Beads | E7870S | 6 rxns | |
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| E7870L | 24 rxns | |
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| E7870X | 96 rxns | |
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| New NEBNext rRNA Depletion Kit V2 (Human/Mouse/Rat) with RNA Sample Purification Beads | E7405S | 6 rxns | |
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| E7405L | 24 rxns | |
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| E7405X | 96 rxns | |
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| New NEBNext rRNA Depletion Kit V2 (Human/Mouse/Rat) | E7400S | 6 rxns | |
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| E7400L | 24 rxns | |
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| E7400X | 96 rxns | |
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| NEW NEBNext rRNA Depletion Kit (Bacteria) with RNA Sample Purification Beads | E7860S | 6 rxns | |
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| E7860L | 24 rxns | |
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| E7860X | 96 rxns | |
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| NEW NEBNext rRNA Depletion Kit (Bacteria) | E7850S | 6 rxns | |
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| E7850L | 24 rxns | |
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| E7850X | 96 rxns | |
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| NEBNext rRNA Depletion Kit (Human/Mouse/Rat) | E6310S | 6 rxns | |
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| E6310L | 24 rxns | |
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| E6310X | 96 rxns | |
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| NEW NEBNext Globin & rRNA Depletion Kit (Human/Mouse/Rat) | E7750S | 6 rxns | |
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| E7750L | 24 rxns | |
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| E7750X | 96 rxns | |
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| NEW NEBNext Globin & rRNA Depletion Kit (Human/Mouse/Rat) with RNA Sample Purification Beads | E7755S | 6 rxns | |
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| E7755L | 24 rxns | |
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| E7755X | 96 rxns | |
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| Related products |
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| NEBNext Ultra Directional RNA Library Prep Kit for Illumina | E7420S | 24 rxns | |
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| E7420L | 96 rxns | |
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| NEBNext Ultra RNA Library Prep Kit for Illumina | E7530S | 24 rxns | |
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| E7530L | 96 rxns | |
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| NEBNext Poly(A) mRNA Magnetic Isolation Module | E7490S | 24 rxns | |
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| E7490L | 96 rxns | |
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| NEW NEBNext Magnetic Separation Rack | S1515S | 1 rack for 24 tubes | |
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Further information can be found in our Technical Resources section or at neb.com
