The new benchmark in NGS Library Prep for Illumina Platforms.
Get even more from less with NEBNext Ultra II!
Are you challenged with trying to obtain higher library yields using ever-decreasing input amounts? Each component in the NEBNext Ultra II DNA Library Prep Kit from NEB has been reformulated, resulting in a several-fold increase in library yield with as little as 500 picograms of input DNA. These advances deliver unprecedented performance, while enabling lower inputs and fewer PCR cycles.
Find all technical information on NEBNext Ultra II Kits here:
- Get more of what you need, with the highest library yields
- Use to generate high quality libraries even when you have only limited amounts of DNA, with inputs as low as 500 pg
- Prepare libraries from ALL of your samples, including GC-rich targets and FFPE DNA samples
- Improve yields for target enrichment applications
- Save time with streamlined workflows, reduced hands-on time, and automation compatibility, and enjoy the flexibility of kit or module format products
NEBNext Ultra II DNA Library Prep Protocol
This video walks you through DNA Library Preparation using the NEBNext Ultra II DNA Library Prep Kit. The video also includes tips for optimization as well as safe stopping points.
12 Quick Tips for NGS Library Preparation
Using beads to clean up your DNA prior to NGS library prep can be quick and easy, if you follow these few, simple tips.
NEBNext Ultra II produces the highest yield libraries from a broad range of input amounts.
Technical Note:
“Improved library preparation with the
NEBNext Ultra II DNA Library Prep Kit for Illumina”
See what others are saying about NEBNext Ultra II:
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„The possibility to use the new NEBNext Ultra II DNA kit has provided us with a critical opportunity to process challenging low-input genomic samples. These samples otherwise didn't yield libraries of adequate complexity, required for exploring their genomes comprehensively. Using the NEBNext Ultra II DNA Kit has alleviated this problem and enabled us to deliver high quality, high content sequencing data that are relevant for our users.“ Dr. Vladimir Benes, Head of GeneCore, EMBL Heidelberg |
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„The protocol is very easy to follow and is a step up from the standard NEB Ultra Kit. Using as little as 500 pg of cell-free DNA input, we were able to generate libraries that were successfully used for subsequent exome capture.“ Dr. Christopher Smith, Post-Doctoral Research Associate at the Cancer Research UK - Cambridge Institute (CRUK-CI) |
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| PRODUCT | Prod.Nr.: | SIZE | PRICE | MORE INFO |
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| NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs Set 2) | E6442S/L | 96/384 rxns |  |
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| NEBNext Magnetic Separation Rack | S1515S | 1 rack | |
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| NEBNext Ultra II FS DNA Library Prep Kit for Illumina | E7780S | 96 rxns | |
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| NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs) | E6440S/L | 96/384 rxns | |
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| NEBNext® Multiplex Oligos for Illumina (Index Primers Set 3) | E7710S/L | 24/96 rxns | |
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| NEBNext® Multiplex Oligos for Illumina (Index Primers Set 4) | E7730S/L | 24/96 rxns | |
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| NEBNext Ultra II FS DNA Library Prep Kit for Illumina | E7805S/L | 24/96 rxns | |
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| NEBNext Ultra II FS DNA Library Prep Kit with Sample Purification Beads | E6177S/L | 24/96 rxns | |
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| NEBNext Ultra II FS DNA Module | E7810S/L | 24/96 rxns | |
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| NEBNext Ultra II DNA Library Prep Kit for Illumina | E7645S/L | 24/96 rxns | |
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| NEW: NEBNext Ultra II DNA Library Prep Kit for Illumina with sample purification beads (SPRIselect Beads) | E7103S/L | 24/96 rxns | |
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| NEBNext Ultra II End Repair/dA-Tailing Module | E7546S/L | 24/96 rxns | |
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| NEBNext Ultra II Ligation Module | E7595S/L | 24/96 rxns | |
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| NEBNext Ultra II Q5 Master Mix | M0544S/L | 50 rxns | |
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Please contact us for a free sample*:
*Samples are limited to 1 piece per working group and as long as stock lasts.
Further information can be found in our Technical Resources section or at neb.com
